Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.

Abstract:

BACKGROUND:The house dust mite (HDM) Dermatophagoides pteronyssinus is a major allergen source eliciting allergic asthma. The aim of the study was to identify new important HDM allergens associated with allergic asthma. METHODS:A cDNA coding for a new mite allergen, designated Der p 21, was isolated using immunoglobulin E (IgE) antibodies from patients with allergic asthma out of a D. pteronyssinus expression cDNA library and expressed in Escherichia coli. RESULTS:Circular dichroism analysis of the purified allergen showed that rDer p 21 (14 726 Da) is one of the few mite allergens with an alpha-helical secondary structure. The protein exhibited high thermal stability and refolding capacity, and, as determined by small angle X-ray scattering, formed a dimer consisting of two flat triangles. rDer p 21 bound high levels of patients' IgE antibodies and showed high allergenic activity in basophil activation experiments. Rabbit anti-Der p 21 IgG antibodies inhibited mite-allergic patients' IgE binding and allowed the ultrastructural localization of the allergen in the midgut (epithelium, lumen and faeces) of D. pteronyssinus by immunogold electron microscopy. Der p 21 revealed sequence homology with group 5 mite allergens, but IgE and IgG reactivity data and cross-inhibition studies identified it as a new mite allergen. CONCLUSIONS:Der p 21 is a new important mite allergen which is liberated into the environment via faecal particles and hence may be associated with allergic asthma.

journal_name

Allergy

journal_title

Allergy

authors

Weghofer M,Dall'Antonia Y,Grote M,Stöcklinger A,Kneidinger M,Balic N,Krauth MT,Fernández-Caldas E,Thomas WR,van Hage M,Vieths S,Spitzauer S,Horak F,Svergun DI,Konarev PV,Valent P,Thalhamer J,Keller W,Valenta R,Vrtal

doi

10.1111/j.1398-9995.2008.01647.x

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

758-67

issue

6

eissn

0105-4538

issn

1398-9995

pii

ALL1647

journal_volume

63

pub_type

杂志文章

相关文献

ALLERGY文献大全
  • The role of diesel exhaust particles and their associated polyaromatic hydrocarbons in the induction of allergic airway disease.

    abstract::The increase in allergic airway disease has paralleled the increase in the use of fossil fuels. Studies were undertaken to examine whether extracts of polyaromatic hydrocarbons (PAH) from diesel exhaust particles (DEP) (PAH-DEP) acted as mucosal adjuvants to help initiate or enhance immunoglobulin E (IgE) production i...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/j.1398-9995.1997.tb04871.x

    authors: Diaz-Sanchez D

    更新日期:1997-01-01 00:00:00

  • Dual cutaneous reactions to anti-human IgE; no change in plasma level of the split product complement C3d or effect of heparin.

    abstract::The possible dependence of the dual cutaneous reaction (DCR) on activation of the complement and coagulation systems was further elucidated. Heterologous whole anti-human IgE was injected intradermally (i.d.) on the volar aspect of the forearms of 17 drug-free healthy volunteers and venous samples for the selected lab...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1985.tb00883.x

    authors: Grönneberg R

    更新日期:1985-11-01 00:00:00

  • Immune dysregulation increases the incidence of delayed-type drug hypersensitivity reactions.

    abstract::Delayed-type, T cell-mediated, drug hypersensitivity reactions are a serious unwanted manifestation of drug exposure that develops in a small percentage of the human population. Drugs and drug metabolites are known to interact directly and indirectly (through irreversible protein binding and processing to the derived ...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/all.14127

    authors: Naisbitt DJ,Olsson-Brown A,Gibson A,Meng X,Ogese MO,Tailor A,Thomson P

    更新日期:2020-04-01 00:00:00

  • Results of biological standardization with standardized allergen preparations.

    abstract::The aim of biological standardization (BS) is to equilibrate the activity (potency) of allergen extracts from different source materials. This was done by performing skin prick tests (SPT) on patients who were sensitive to one of the following 10 allergens: Birch, alder, hazel, timothy, rye grass, velvet grass, cultiv...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1398-9995.1987.tb02368.x

    authors: Dreborg S,Belin L,Eriksson NE,Grimmer O,Kunkel G,Malling HJ,Nilsson G,Sjögren I,Zetterström O

    更新日期:1987-02-01 00:00:00

  • The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma.

    abstract::A family of widely expressed ORM-like (ORMDL) proteins has been recently linked to asthma in genomewide association studies in humans and extensively explored in in vivo studies in mice. ORMDL proteins are key regulators of serine palmitoyltransferase, an enzyme catalyzing the initial step of sphingolipid biosynthesis...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/all.12877

    authors: Paulenda T,Draber P

    更新日期:2016-07-01 00:00:00

  • Mold-sensitivity in children with moderate-severe asthma is associated with HLA-DR and HLA-DQ.

    abstract:BACKGROUND:Several epidemiologic studies in the United States and Europe have linked Alternaria sensitivity to both persistence and severity of asthma. In this study, we examined T cell responses and HLA class II alleles in children with moderate-severe asthma. METHODS:Ninety-six children with moderate-severe asthma w...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2010.02382.x

    authors: Knutsen AP,Vijay HM,Kumar V,Kariuki B,Santiago LA,Graff R,Wofford JD,Shah MR

    更新日期:2010-11-01 00:00:00

  • Airborne pollen levels and drug consumption for seasonal allergic rhinoconjunctivitis: a 10-year study in France.

    abstract:BACKGROUND:Few time-series studies, and none lasting longer than 4 years, have investigated the etiology of treated seasonal allergic rhinoconjunctivitis (SAR) on the basis of anti-allergic medication prescriptions. The aim of this article was to study the short-term relationship between pollen exposure and drug-treate...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12522

    authors: Caillaud DM,Martin S,Ségala C,Vidal P,Lecadet J,Pellier S,Rouzaire P,Tridon A,Evrard B

    更新日期:2015-01-01 00:00:00

  • Eicosanoid mediator profiles in different phenotypes of nonsteroidal anti-inflammatory drug-induced urticaria.

    abstract:BACKGROUND:The role of arachidonic acid metabolites in NSAID-induced hypersensitivity has been studied in depth for NSAID-exacerbated respiratory disease (NERD) and NSAID-exacerbated cutaneous disease (NECD). However, no information is available for NSAID-induced urticarial/angioedema (NIUA), despite it being the most ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.13725

    authors: Doña I,Jurado-Escobar R,Perkins JR,Ayuso P,Plaza-Serón MC,Pérez-Sánchez N,Campo P,Bogas-Herrera G,Bartra J,Torres MJ,Sanak M,Cornejo-García JA

    更新日期:2019-06-01 00:00:00

  • Treatment of hay fever with sodium cromoglycate, hyposensitization, or a combination.

    abstract::In 77 consecutive patients with grass pollen rhinitis as the only allergic disease, treatment with sodium cromoglycate (SCG) nasal spray, hyposensitization with aluminium hydroxide adsorbed extract of grass pollen as perennial treatment, and a combination of the two was compared. Specific serum-IgE, IgG and IgG4 were ...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1987.tb02220.x

    authors: Andersen NH,Jeppesen F,Schiøler T,Osterballe O

    更新日期:1987-07-01 00:00:00

  • Effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL-2H3) cells: the structure-inhibitory activity relationship.

    abstract::We studied the effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL-2H3) cells. Among tea polyphenols, (-)- epigallocatechin gallate (EGCG) most strongly and dose-dependently inhibited histamine release from cells stimulated with a calcium ionophore, A23187. (-)-Epigallocatechin (EGC) and (...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1997.tb02546.x

    authors: Matsuo N,Yamada K,Shoji K,Mori M,Sugano M

    更新日期:1997-01-01 00:00:00

  • Seasonal allergic rhinitis and depot injection of a corticosteroid. Evaluation of the efficacy of medication early and late in the season based on detailed symptom recording.

    abstract::It was the aim of this investigation to study the efficacy of a corticosteroid given as a depot injection in seasonal allergic rhinitis, the efficacy profile with regard to specific symptoms, and whether the injection is best given early in the season or later at the peak of the pollen count. An injection of 80 mg met...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1398-9995.1987.tb02183.x

    authors: Borum P,Grønborg H,Mygind N

    更新日期:1987-01-01 00:00:00

  • Safety, efficacy and repeatability of a novel house dust mite allergen challenge technique in the Fraunhofer allergen challenge chamber.

    abstract:BACKGROUND:Efficacy testing of immunotherapy in field studies is often hampered by variation of airborne allergens. Standardized allergen exposure in challenge chamber settings might be an alternative. Therefore, we developed a universal technique to create an atmosphere loaded with allergen particles of adjustable siz...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12947

    authors: Lueer K,Biller H,Casper A,Windt H,Mueller M,Badorrek P,Haefner D,Framke T,Koch A,Ziehr H,Krug N,Koch W,Hohlfeld JM

    更新日期:2016-12-01 00:00:00

  • The effect of fluticasone propionate aqueous nasal spray on nasal mucosal inflammation in perennial allergic rhinitis.

    abstract::Mast cell degranulation, and the subsequent recruitment of infiltrating inflammatory cells, such as eosinophils, into the nasal mucosa has long been considered the most important model to explain allergic rhinitis. Several studies show a decrease in the number of eosinophils and possibly also mast cells during local c...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1995.tb02737.x

    authors: Godthelp T,Holm AF,Blom H,Klein-Jan A,Rijntjes E,Fokkens WJ

    更新日期:1995-01-01 00:00:00

  • Full automation in allergy testing: measurement of specific IgE by the ENEA System.

    abstract:UNLABELLED:We evaluated the ENEA System, a fully automated instrument for the measurement of specific IgE antibodies. The instrument dispenses sera and reagents, incubates, washes, and reads and prints results automatically. The "core" of the instrument is the reactive unit called ACE (Allergy Chamber Enzymatic), which...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1995.tb01139.x

    authors: Plebani M,Faggian D,Borghesan F

    更新日期:1995-03-01 00:00:00

  • Influence of food on the absorption rate and bioavailability of a sustained release theophylline preparation.

    abstract::Six children and six adults were given a single dose of a sustained release theophylline preparation (Nuelin Retard) after an overnight fast and later after a standardized breakfast. Food significantly reduced the absorption rate of theophylline whereas the extent of absorption of theophylline was unaffected by food. ...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1982.tb02336.x

    authors: Pedersen S,Moeller-Petersen J

    更新日期:1982-10-01 00:00:00

  • Undernotification of anaphylaxis deaths in Brazil due to difficult coding under the ICD-10.

    abstract:BACKGROUND:Undernotification is well recognized as a key challenge to the study of anaphylaxis mortality, but it is seldom mentioned that one of its reasons is the difficult coding of the condition under the tenth revision of the international classification of diseases (ICD-10), given that there are no anaphylaxis-spe...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2012.02829.x

    authors: Tanno LK,Ganem F,Demoly P,Toscano CM,Bierrenbach AL

    更新日期:2012-06-01 00:00:00

  • Markers for eosinophils and T-lymphocytes as predictors of late asthmatic response.

    abstract::Bronchial allergen challenge was performed on 12 allergic asthmatics during a stable phase of their disease. After resolution of the immediate bronchial response, fractional lung lavage was performed twice, two and 24 h post-challenge. The recovery of eosinophils, eosinophil cationic protein (ECP), eosinophil chemotac...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1993.tb04708.x

    authors: Schmekel B,Venge P

    更新日期:1993-01-01 00:00:00

  • Comparison of FEV1 and specific airway conductance in assessing airway response to occupational agents.

    abstract:BACKGROUND:There is theoretical evidence that specific airway conductance (SGaw) could be more reliable than forced expiratory volume in 1 s (FEV1) for assessing changes in airway calibre. We investigated the changes in FEV1 and SGaw when assessing bronchial responses to occupational agents. METHODS:SGaw and FEV1 were...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1046/j.1398-9995.2003.00313.x

    authors: Larbanois A,Delwiche JP,Jamart J,Vandenplas O

    更新日期:2003-12-01 00:00:00

  • Multiple atopy phenotypes and their associations with asthma: similar findings from two birth cohorts.

    abstract:BACKGROUND:Although atopic sensitization is one of the strongest risk factors for asthma, its relationship with asthma is poorly understood. We hypothesize that 'atopy' encompasses multiple sub-phenotypes that relate to asthma in different ways. METHODS:In two population-based birth cohorts (Manchester and Isle of Wig...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12134

    authors: Lazic N,Roberts G,Custovic A,Belgrave D,Bishop CM,Winn J,Curtin JA,Hasan Arshad S,Simpson A

    更新日期:2013-06-01 00:00:00

  • IgE-binding components of cultured human keratinocytes in atopic eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur.

    abstract:BACKGROUND:Atopic eczema/dermatitis syndrome (AEDS) patients display immunoglobulin E (IgE) reactivity to several antigens, e.g. saprophytic yeasts as Malassezia furfur. AEDS patients also show IgE autoreactivity towards cells of their own tissue including epidermis. PURPOSE OF THE STUDY:The aim of this study was to i...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1046/j.1398-9995.2003.00330.x

    authors: Kortekangas-Savolainen O,Peltonen S,Pummi K,Kalimo K,Savolainen J

    更新日期:2004-02-01 00:00:00

  • Multiplexed immunoglobulin E sensitization in relation to exhaled nitric oxide in a population sample of children.

    abstract::This study investigated the relationship between the specific immunoglobulin E (IgE) profile for 40 allergens using a novel microarray technique (BioIC) and fraction of exhaled nitric oxide (FeNO) in a population sample of 1321 children. Significant positive associations were found between FeNO and sensitization to mi...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12378

    authors: Yao TC,Tsai HJ,Tu YL,Chang SW,Hua MC,Liao SL,Tsai MH,Chiu CY,Lai SH,Yeh KW,Huang JL,PATCH study group.

    更新日期:2014-05-01 00:00:00

  • Allergen-induced mediator release tests.

    abstract::The diagnosis of allergic reactions in clinical practice is based on both clinical history and the determination of specific immunoglobulin E (IgE), either in the serum or on skin mast cells. However, for various reasons, identification of the causative factors is not possible in all the cases. Moreover, not all aller...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1034/j.1398-9995.2003.00253.x

    authors: Demoly P,Lebel B,Arnoux B

    更新日期:2003-07-01 00:00:00

  • Four methods of sampling for dust mite allergen: differences in 'dust'.

    abstract:BACKGROUND:Measurement of exposure to the dust mite allergen Der p 1 is important in asthma research and is potentially useful in managing asthma. As no single measure can capture all characteristics of an exposure, it is important to recognize differences in the available methods of measuring exposure to Der p 1. MET...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2003.00228.x

    authors: Tovey ER,Mitakakis TZ,Sercombe JK,Vanlaar CH,Marks GB

    更新日期:2003-08-01 00:00:00

  • Frequency and intensity of responses to mite patch tests are lower in nonatopic subjects with respect to patients with atopic dermatitis.

    abstract:BACKGROUND:So far the issue of patch tests with mite allergens in subjects not affected by atopic dermatitis (AD) has been poorly investigated. The aim of this study was to assess the frequency and intensity of responses to atopy patch tests with Dermatophagoides in non-AD subjects, and to compare them to the ones obse...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1034/j.1398-9995.2003.00142.x

    authors: Seidenari S,Giusti F,Pellacani G,Bertoni L

    更新日期:2003-05-01 00:00:00

  • A double-blind controlled trial of elemental diet in severe, perennial asthma.

    abstract::In a double-blind controlled trial 41 hospitalized adults with severe, perennial asthma of unknown aetiology were allocated to either an antigen-free elemental diet (Vivasorb) or control diet, i.e. blended ordinary hospital food, for 2 weeks. At the time of entry into the trial all patients were in an active but stabl...

    journal_title:Allergy

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1398-9995.1981.tb01572.x

    authors: Høj L,Osterballe O,Bundgaard A,Weeke B,Weiss M

    更新日期:1981-05-01 00:00:00

  • Ingestive and inhalative allergy to the mushroom Boletus edulis.

    abstract::This is the first report on inhalative and ingestive allergy to the common edible mushroom Boletus edulis (Be) (English, edible boletus; German, Steinpilz; French, bolet; Italian, porcino or boleto) belonging to the class Basidiomycetes. Four cases observed in our allergy unit are presented, showing different clinical...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.1997.tb01232.x

    authors: Torricelli R,Johansson SG,Wüthrich B

    更新日期:1997-07-01 00:00:00

  • Breastfeeding and allergic disease: a multidisciplinary review of the literature (1966-2001) on the mode of early feeding in infancy and its impact on later atopic manifestations.

    abstract:BACKGROUND:Strategies to prevent children from developing allergy have been elaborated on the basis of state-of-the-art reviews of the scientific literature regarding pets and allergies, building dampness and health, and building ventilation and health. A similar multidisciplinary review of infant feeding mode in relat...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1034/j.1398-9995.2003.00264.x

    authors: van Odijk J,Kull I,Borres MP,Brandtzaeg P,Edberg U,Hanson LA,Høst A,Kuitunen M,Olsen SF,Skerfving S,Sundell J,Wille S

    更新日期:2003-09-01 00:00:00

  • Real-life study showing uncontrolled rhinosinusitis after sinus surgery in a tertiary referral centre.

    abstract:RATIONALE:The European Position Paper on Sinusitis (EPOS) guidelines provide composite criteria to evaluate chronic rhinosinusitis (CRS) control, taking into consideration the severity of patients' symptoms, aspect of nasal mucosa and medical intake as parameters of CRS control. OBJECTIVES:To study the degree of CRS c...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/all.12983

    authors: van der Veen J,Seys SF,Timmermans M,Levie P,Jorissen M,Fokkens WJ,Hellings PW

    更新日期:2017-02-01 00:00:00

  • Under-report and underdiagnosis of chronic respiratory diseases in an African country.

    abstract:BACKGROUND:Chronic respiratory diseases (CRD) are greatly underestimated. The aim of this study was to assess the burden associated with reported CRD and chronic obstructive pulmonary disease, as defined on the basis of various standardized criteria, by estimating their point prevalence in a sample of individuals atten...

    journal_title:Allergy

    pub_type: 杂志文章

    doi:10.1111/j.1398-9995.2009.01956.x

    authors: Martins P,Rosado-Pinto J,do Céu Teixeira M,Neuparth N,Silva O,Tavares H,Spencer JL,Mascarenhas D,Papoila AL,Khaltaev N,Annesi-Maesano I

    更新日期:2009-07-01 00:00:00

  • The axis of the receptor for advanced glycation endproducts in asthma and allergic airway disease.

    abstract::Asthma is a generalized term that describes a scope of distinct pathologic phenotypes of variable severity, which share a common complication of reversible airflow obstruction. Asthma is estimated to affect almost 400 million people worldwide, and nearly ten percent of asthmatics have what is considered "severe" disea...

    journal_title:Allergy

    pub_type: 杂志文章,评审

    doi:10.1111/all.14600

    authors: Perkins TN,Donnell ML,Oury TD

    更新日期:2020-09-25 00:00:00