当前位置： SCI文献检索 > NEUROSCIENCE期刊下所有文献 > Targeted silencing of TrkA expression in rat forebrain neurons via the p75 receptor.

Targeted silencing of TrkA expression in rat forebrain neurons via the p75 receptor.

Abstract：

:Basal forebrain neurons express the neurotrophin receptors, p75NTR and tyrosine kinase receptor A (TrkA). We tested the hypothesis that impairment of memory in rats could be achieved by RNA interference (RNAi) -induced silencing of TrkA specifically within these neurons. A novel fusogenic, karyophilic immunoporter (fkAb(p75)-ipr) was constructed from the antibody, MC192 (monoclonal antibody to the rat neurotrophin receptor p75NTR, Ab(p75)), poly-l-lysine together with the hemagglutinin 2 and VP1 nuclear localization peptides of influenza and SV40 virus, respectively. Plasmid DNA constructs containing short hairpin sequences inhibitory to tyrosine kinase receptor A expression (TrkAi) and the gene encoding cGFP (green fluorescent protein from coral fish) was produced. These TrkAi plasmids were mixed with the immunoporter, forming the immunogene, TrkAi-fkAb(p75). A control TrkAsc complexed with fkAb(p75) (TrkAsc-fkAb(p75)) immunogene was constructed from a scrambled sequence (TrkAsc) and fkAb(p75)-ipr. Rats were infused using an osmotic mini-pump into the third ventricle with either TrkAi-fkAb(p75) or TrkAsc-fkAb(p75). Naive rats were also included as additional controls. After 7 days, examination of gene expression on forebrain sections of some rats revealed cGFP expression in TrkA neurons. Fifteen to 19 days after infusion, rats were tested in a Morris water maze apparatus. Animals that received TrkAi-fkAb(p75) showed significantly impaired spatial memory learning ability compared with naive or TrkAsc-fkAb(p75)-treated rats. Western blot and immunofluorescence analysis showed that TrkA protein levels and numbers of TrkA positive neurons were reduced by 60% and 55% respectively in TrkAi-fkAb(p75)-infused rats compared with infused controls or naive animals. We conclude that p75-receptor-mediated RNAi-induced silencing of genes offers a novel and powerful way to study the function of specific endogenous genes within distinct neuronal subpopulations of the brain.

journal_name

Neuroscience

journal_title

Neuroscience

authors

Berhanu DA,Rush RA

doi

10.1016/j.neuroscience.2008.03.025

subject

Has Abstract

pub_date

2008-06-02 00:00:00

pages

1115-25

issue

4

eissn

0306-4522

issn

1873-7544

pii

S0306-4522(08)00366-7

journal_volume

153

pub_type

杂志文章

相关文献

NEUROSCIENCE文献大全
  • Preconception paternal stress in rats alters dendritic morphology and connectivity in the brain of developing male and female offspring.

    abstract：:The goal of this research was to examine the effect of preconception paternal stress (PPS) on the subsequent neurodevelopment and behavior of male and female offspring. Prenatal (gestational) stress has been shown to alter brain morphology in the developing brain, and is presumed to be a factor in the development of s...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2015.06.058

    authors： Harker A,Raza S,Williamson K,Kolb B,Gibb R

    更新日期：2015-09-10 00:00:00

  • Extrinsic origins of the somatostatin and neuropeptide Y innervation of the rat basolateral amygdala.

    abstract：:The amygdalar basolateral nuclear complex (BLC) is a cortex-like structure that receives inputs from many cortical areas. It has long been assumed that cortico-amygdalar projections, as well as inter-areal intracortical connections, arise from cortical pyramidal cells. However, recent studies have shown that GABAergic...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2015.03.004

    authors： McDonald AJ,Zaric V

    更新日期：2015-05-21 00:00:00

  • Excitatory amino acid receptors in the periaqueductal gray mediate the cardiovascular response evoked by activation of dorsomedial hypothalamic neurons.

    abstract：:Neurons in the region of dorsomedial hypothalamus are involved in the organization of the physiological responses to emotional stress. We have recently shown that the cardiovascular response evoked by activation of dorsomedial hypothalamus neurons is largely dependent on a synaptic relay with the lateral/dorsolateral ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2005.12.041

    authors： da Silva LG Jr,Menezes RC,Villela DC,Fontes MA

    更新日期：2006-01-01 00:00:00

  • Differential sensitivity of the caudal and rostral nucleus accumbens to the rewarding effects of a H1-histaminergic receptor blocker as measured with place-preference and self-stimulation behavior.

    abstract：:A recent series of studies in rats has demonstrated positively reinforcing and memory enhancing effects following lesions of the nucleus tuberomammillaris, which is the only known source of neuronal histamine. The aim of the present experiments was to assess whether inhibition of histaminergic neurotransmission in the...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(99)00309-7

    authors： Zimmermann P,Privou C,Huston JP

    更新日期：1999-01-01 00:00:00

  • Caffeine-induced calcium release from internal stores in cultured rat sensory neurons.

    abstract：:Free intracellular calcium concentration ([Ca2+]in) was recorded at 22 degrees C by means of Indo-1 or Fura-2 single-cell microfluorometry in cultured dorsal root ganglion neurons obtained from neonatal rats. The resting [Ca2+]in in dorsal root ganglion neurons was 73 +/- 21 nM (mean +/- S.D., n = 94). Fast applicatio...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(93)90029-f

    authors： Usachev Y,Shmigol A,Pronchuk N,Kostyuk P,Verkhratsky A

    更新日期：1993-12-01 00:00:00

  • Acute homocysteine administration impairs memory consolidation on inhibitory avoidance task and decreases hippocampal brain-derived neurotrophic factor immunocontent: prevention by folic acid treatment.

    abstract：:In the present study, we first investigated the effect of single homocysteine administration on consolidation of short- and long-term memories of inhibitory avoidance task in Wistar rats. We also measured brain-derived neurotrophic factor levels in the hippocampus and parietal cortex of rats. The influence of pretreat...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2009.07.023

    authors： Matté C,Pereira LO,Dos Santos TM,Mackedanz V,Cunha AA,Netto CA,Wyse AT

    更新日期：2009-11-10 00:00:00

  • Raphe magnus/pallidus neurons regulate tail but not mesenteric arterial blood flow in rats.

    abstract：:In urethane-anesthetized rats with body temperature maintained at 39-40 degrees C, electrical stimulation of raphe magnus/pallidus/parapyramidal region within 0.5 mm of the ventral medullary surface reduced arterial blood flow to the tail cutaneous bed (measured with a chronically implanted Doppler ultrasonic flowmete...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(01)00251-2

    authors： Blessing WW,Nalivaiko E

    更新日期：2001-01-01 00:00:00

  • Brain diffusivity pattern is individual-specific information.

    abstract：:The human brain is composed of complex networks of 100 billion neurons that underlie its higher functions. The set of neural connections in the brain has recently attracted growing interest from the scientific community. It is important to identify individual differences in these neural connections to study the backgr...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2015.06.032

    authors： Takao H,Hayashi N,Ohtomo K

    更新日期：2015-08-20 00:00:00

  • The role of Efr3a in age-related hearing loss.

    abstract：:Efr3a has been found to be involved in the functional maintenance and structural degeneration of sensory and motor nervous tissues. Our previous data have suggested that Efr3a may be associated with the initiation of the degeneration of spiral ganglion neurons (SGNs). In this study, we used Efr3a knockdown (Efr3a KD) ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2016.11.013

    authors： Hu H,Ma Y,Ye B,Wang Q,Yang T,Lv J,Shi J,Wu H,Xiang M

    更新日期：2017-01-26 00:00:00

  • Impaired release of beta-endorphin in response to serotonin in a rat model of depression.

    abstract：:Involvement of both the serotonergic and the endogenous opioid systems in the onset of depressive behavior has been suggested. Previously we showed that serotonin (5-hydroxytryptamine) facilitates beta-endorphin release in the nucleus accumbens (NAcc). Herein, the microdialysis method was used to assess in vivo the ef...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(01)00612-1

    authors： Zangen A,Nakash R,Roth-Deri I,Overstreet DH,Yadid G

    更新日期：2002-01-01 00:00:00

  • The light chain of tetanus toxin inhibits calcium-dependent vasopressin release from permeabilized nerve endings.

    abstract：:The effects of tetanus toxin and its light and heavy chain subunits on vasopressin release were investigated in digitonin-permeabilized neurosecretory nerve terminals isolated from the neural lobe of the rat pituitary gland. Exocytosis was induced by challenging the permeabilized nerve endings with micromolar calcium ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(92)90068-d

    authors： Dayanithi G,Weller U,Ahnert-Hilger G,Link H,Nordmann JJ,Gratzl M

    更新日期：1992-01-01 00:00:00

  • Effect of lidocaine on dynamic changes in cortical reduced nicotinamide adenine dinucleotide fluorescence during transient focal cerebral ischemia in rats.

    abstract：:Rats were subjected to 90min of focal ischemia by occluding the left middle cerebral and both common carotid arteries. The dynamic changes in the formation of brain ischemic areas were analyzed by measuring the direct current (DC) potential and reduced nicotinamide adenine dinucleotide (NADH) fluorescence with ultravi...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2013.01.010

    authors： Naito H,Takeda Y,Danura T,Kass IS,Morita K

    更新日期：2013-04-03 00:00:00

  • Reciprocal changes in dopamine responsiveness in the nucleus accumbens shell and core and in the dorsal caudate-putamen in rats sensitized to morphine.

    abstract：:In this study, we describe a model of opiate sensitization characterized by a brief schedule of treatment with repeated morphine administrations. In this model, we investigated the changes produced by repeated morphine treatment on dopamine transmission at the level of the two major terminal dopaminergic areas, the do...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(98)00466-7

    authors： Cadoni C,Di Chiara G

    更新日期：1999-05-01 00:00:00

  • Regulation of ischemic cell death by glucocorticoids and adrenocorticotropic hormone.

    abstract：:Transient global ischemia results in delayed selective neuronal death of hippocampal CA1 pyramidal cells. Glucocorticoids increase and adrenalectomy decreases the rate of neuronal death; however, they also produce changes in brain temperature, serum glucose and adrenocorticotropic hormone levels. In order to understan...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(98)00213-9

    authors： Antonawich FJ,Miller G,Rigsby DC,Davis JN

    更新日期：1999-01-01 00:00:00

  • Arginine vasopressin regulated ASCT1 expression in astrocytes from stroke-prone spontaneously hypertensive rats and congenic SHRpch1_18 rats.

    abstract：:In stroke-prone spontaneously hypertensive rats (SHRSP/Izm), ischemia induces swelling of astrocytes, a process that subsequently leads to neuronal death. Following ischemic insult, arginine vasopressin (AVP) can induce edema and l-serine released by astrocytes supports the survival of neuronal cells. The purpose of t...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2014.02.039

    authors： Yamagata K,Yamamoto M,Kawakami K,Ohara H,Nabika T

    更新日期：2014-05-16 00:00:00

  • Central or peripheral delivery of an adenosine A1 receptor agonist improves mechanical allodynia in a mouse model of painful diabetic neuropathy.

    abstract：:Diabetic peripheral neuropathy is a common complication of diabetes mellitus, and a significant proportion of individuals suffer debilitating pain that significantly affects their quality of life. Unfortunately, symptomatic treatment options have limited efficacy, and often carry significant risk of systemic adverse e...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2014.10.065

    authors： Katz NK,Ryals JM,Wright DE

    更新日期：2015-01-29 00:00:00

  • Inhibitory Effects of Cyclopiazonic Acid on the Pacemaker Current in Sinoatrial Nodal Cells.

    abstract：OBJECTIVE:The spontaneous action potential of isolated sinoatrial node (SAN) cells is regulated by a coupled-clock system of two clocks: the calcium clock and membrane clock. However, it remains unclear whether calcium clock inhibitors have a direct effect on the membrane clock. The purpose of this study was to investi...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2020.01.018

    authors： Chen Q,Chen JQ,Zhu PL,Wu HL,Xie XT,Dong Y,Xiang GJ,Chen MY,Li Y,Zhang JC

    更新日期：2020-05-01 00:00:00

  • A new approach with less damage: intranasal delivery of tetracycline-inducible replication-defective herpes simplex virus type-1 vector to brain.

    abstract：:Gene therapy holds great potential for treating neurological disorders. However, delivering gene vectors to the brain has been either invasive or inefficacious in most studies to date. The aim of this study was to develop a safe and efficacious strategy for delivering gene vectors to the brain. A tetracycline-inducibl...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2011.10.043

    authors： Jiang Y,Wei N,Zhu J,Zhai D,Wu L,Chen M,Xu G,Liu X

    更新日期：2012-01-10 00:00:00

  • The visceral sector of the thalamic reticular nucleus in the rat.

    abstract：:Visceral sensory perception is subjected to modulation by attention or distraction, like other sensory systems. The thalamic reticular nucleus is a key region in selective attention, effecting a change in the mode of thalamocortical transmission. Each major thalamocortical system is connected with a particular sector ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/s0306-4522(01)00316-5

    authors： Stehberg J,Acuña-Goycolea C,Ceric F,Torrealba F

    更新日期：2001-01-01 00:00:00

  • Developmental changes in the expression of calbindin and potassium-channel subunits Kv3.1b and Kv3.2 in mouse Renshaw cells.

    abstract：:One class of spinal interneurons, the Renshaw cells, is able to discharge at very high frequencies in adult mammals. Neuronal firing at such high frequencies requires voltage-gated potassium channels to rapidly repolarize the membrane potential after each action potential. We sought to establish the pattern of express...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2005.12.048

    authors： Song ZM,Hu J,Rudy B,Redman SJ

    更新日期：2006-05-12 00:00:00

  • Loss of Sodium-Activated Potassium Channel Slack and FMRP Differentially Affect Social Behavior in Mice.

    abstract：:The sodium-activated potassium channel Slack (Slo2.2) is widely expressed in central and peripheral neurons where it is supposed to shape firing properties important for neuronal excitability. Slack activity is enhanced by interaction with the Fragile-X-Mental-Retardation-Protein (FMRP) and loss of FMRP leads to decre...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2018.05.040

    authors： Bausch AE,Ehinger R,Straubinger J,Zerfass P,Nann Y,Lukowski R

    更新日期：2018-08-01 00:00:00

  • Abnormal proprioceptive-motor integration contributes to hypometric postural responses of subjects with Parkinson's disease.

    abstract：:Subjects with Parkinson's disease exhibit abnormally short compensatory steps in response to external postural perturbations. We examined whether: (1) Parkinson's disease subjects exhibit short compensatory steps due to abnormal central proprioceptive-motor integration, (2) this proprioceptive-motor deficit can be ove...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2006.04.014

    authors： Jacobs JV,Horak FB

    更新日期：2006-08-25 00:00:00

  • Modulation of septo-hippocampal Theta activity by GABAA receptors: an experimental and computational approach.

    abstract：:Theta frequency oscillation of the septo-hippocampal system has been considered as a prominent activity associated with cognitive function and affective processes. It is well documented that anxiolytic drugs diminish septo-hippocampal oscillatory Theta activity contributing to their either therapeutic or unwanted side...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.03.043

    authors： Hajós M,Hoffmann WE,Orbán G,Kiss T,Erdi P

    更新日期：2004-01-01 00:00:00

  • Estradiol influences oxytocin-immunoreactive brain systems.

    abstract：:The rat brain was examined immunocytochemically for estrogen-dependent changes of oxytocin immunoreactivity at the light microscopical level. Ovariectomized rats were treated with subcutaneous silastic implants with estradiol, or empty implants as controls for 2 days (short term treatment). Another group of rats was i...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/0306-4522(88)90022-x

    authors： Jirikowski GF,Caldwell JD,Pedersen CA,Stumpf WE

    更新日期：1988-04-01 00:00:00

  • Hippocampal neuroplasticity in major depressive disorder.

    abstract：:One of the most replicated findings has been that hippocampus volume is decreased in patients with major depressive disorder (MDD). Recent volumetric magnetic resonance imaging (MRI) studies suggest that localized differences in hippocampal volume may be more prominent than global differences. Preclinical and post-mor...

    journal_title：Neuroscience

    pub_type： 杂志文章,评审

    doi：10.1016/j.neuroscience.2015.04.047

    authors： Malykhin NV,Coupland NJ

    更新日期：2015-11-19 00:00:00

  • Central projections of sensory innervation of the rat superior sagittal sinus.

    abstract：:The central projections of the rat superior sagittal sinus (SSS) sensory innervation were studied by transganglionic tract tracing techniques. Cholera toxin subunit b (CTb) or wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) was applied on the overlying dura of the SSS and labeled terminations in the b...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2004.07.045

    authors： Liu Y,Broman J,Edvinsson L

    更新日期：2004-01-01 00:00:00

  • Simultaneous estimation of global background synaptic inhibition and excitation from membrane potential fluctuations in layer III neurons of the rat entorhinal cortex in vitro.

    abstract：:It is becoming clear that the detection and integration of synaptic input and its conversion into an output signal in cortical neurons are strongly influenced by background synaptic activity or "noise." The majority of this noise results from the spontaneous release of synaptic transmitters, interacting with ligand-ga...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2007.05.016

    authors： Greenhill SD,Jones RS

    更新日期：2007-07-29 00:00:00

  • Age-related audiovisual interactions in the superior colliculus of the rat.

    abstract：:It is well established that multisensory integration is a functional characteristic of the superior colliculus that disambiguates external stimuli and therefore reduces the reaction times toward simple audiovisual targets in space. However, in a condition where a complex audiovisual stimulus is used, such as the optic...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2016.01.058

    authors： Costa M,Piché M,Lepore F,Guillemot JP

    更新日期：2016-04-21 00:00:00

  • Effect of fatigue on the precision of a whole-body pointing task.

    abstract：:We addressed the issue of the possible degradation of the aiming precision of a whole-body pointing task, when movement coordination is deranged by selective fatigue of the postural task component. The protocol involved continuous repetition (0.1 Hz frequency) of rapid whole-body pointing trials toward a target locate...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2005.12.060

    authors： Schmid M,Schieppati M,Pozzo T

    更新日期：2006-01-01 00:00:00

  • Phenylbutyric acid prevents rats from electroconvulsion-induced memory deficit with alterations of memory-related proteins and tau hyperphosphorylation.

    abstract：:Electroconvulsive therapy has been commonly applied in the treatment of refractory depression, but its cognitive side effects are noticed and restrict its application. The molecular mechanisms underlying the side effects remain elusive, and there is no efficient prevention. By employing a recognized electroconvulsive ...

    journal_title：Neuroscience

    pub_type： 杂志文章

    doi：10.1016/j.neuroscience.2010.03.060

    authors： Yao Z,Guo Z,Yang C,Tian Q,Gong CX,Liu G,Wang JZ

    更新日期：2010-06-30 00:00:00