Abstract：
:MSH5, a member of the MutS homolog DNA mismatch repair protein family, has been shown to be required for proper homologous chromosome recombination in diverse organisms such as mouse, budding yeast and Caenorhabditis elegans. In this paper, we show that a mutant Arabidopsis plant carrying the putative disrupted AtMSH5 gene exhibits defects during meiotic division, producing a proportion of nonviable pollen grains and abnormal embryo sacs, and thereby leading to a decrease in fertility. AtMSH5 expression is confined to meiotic floral buds, which is consistent with a possible role during meiosis. Cytological analysis of male meiosis revealed the presence of numerous univalents from diplotene to metaphase I, which were associated with a great reduction in chiasma frequencies. The average number of residual chiasmata in the mutant is reduced to 2.54 per meiocyte, which accounts for approximately 25% of the amount in the wild type. Here, quantitative cytogenetical analysis reveals that the residual chiasmata in Atmsh5 mutants are randomly distributed among meiocytes, suggesting that AtMSH5 has an essential role during interference-sensitive chiasma formation. Taken together, the evidence indicates that AtMSH5 promotes homologous recombination through facilitating chiasma formation during prophase I in Arabidopsis.
journal_name
Cell Resjournal_title
Cell researchauthors
Lu X,Liu X,An L,Zhang W,Sun J,Pei H,Meng H,Fan Y,Zhang Cdoi
10.1038/cr.2008.44subject
Has Abstractpub_date
2008-05-01 00:00:00pages
589-99issue
5eissn
1001-0602issn
1748-7838pii
cr200844journal_volume
18pub_type
杂志文章相关文献
CELL RESEARCH文献大全abstract：:Recent studies on enzymes and reader proteins for histone crotonylation support a function of histone crotonylation in transcription. However, the enzyme(s) responsible for histone decrotonylation (HDCR) remains poorly defined. Moreover, it remains to be determined if histone crotonylation is physiologically significa...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2017.68
更新日期：2017-07-01 00:00:00
abstract：:The pituitary adenylate cyclase-activating polypeptide type I receptor (PAC1R) belongs to the secretin receptor family and is widely distributed in the central neural system and peripheral organs. Abnormal activation of the receptor mediates trigeminovascular activation and sensitization, which is highly related to mi...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/s41422-020-0280-2
更新日期：2020-05-01 00:00:00
abstract：:Kinesins are microtubule-based motors involved in various intracellular transports. Neurons, flagellated cells, and pigment cells have been traditionally used as model systems to study the cellular functions of kinesins. Here, we report silkworm posterior silkgland (PSG), specialized cells with an extensive endomembra...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2010.47
更新日期：2010-06-01 00:00:00
abstract：:DNA methylation is a fundamental epigenetic mechanism governing regulation of gene expression during mammalian development. A recent study published in Nature shows a novel long noncoding RNA (lncRNA) arising from the CEBPA gene locus (termed ecCEBPA) that is critical for regulation of DNA methylation at this site thr...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2014.13
更新日期：2014-03-01 00:00:00
abstract：:Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report genome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-geno...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2017.25
更新日期：2017-04-01 00:00:00
abstract：:Transforming growth factor beta (TGF-beta) carries out tumor suppressor activity in epithelial and lymphoid cells, whereas telomerase is required for most cancers. Although the molecular mechanisms by which TGF-beta acts as a tumor suppressor are yet to be fully established, a link between TGFb and its tumor suppresso...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/sj.cr.7310023
更新日期：2006-02-01 00:00:00
abstract：:The endoplasmic reticulum (ER) is composed of the nuclear envelope, perinuclear sheets and a peripheral tubular network. The peripheral ER and mitochondria form tight contacts at specific subdomains, which coordinate the functions of the two organelles and are required for multiple cellular processes such as Ca2+ tran...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/s41422-018-0065-z
更新日期：2018-08-01 00:00:00
abstract：:A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290182
更新日期：2003-10-01 00:00:00
abstract：:Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy with a complex tumor ecosystem. How the interplay between tumor cells, EBV, and the microenvironment contributes to NPC progression and immune evasion remains unclear. Here we performed single-cell RNA sequencing on ~104,000 cells from...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/s41422-020-00402-8
更新日期：2020-11-01 00:00:00
abstract：:Various systems by using electric pulse, calcium, or polyethylene glycol have been developed in the past decade for the in vitro fusion of plant gametes. These in vitro systems provide a new way to study the fertilization mechanisms of plants. In this study, we developed a bovine serum albumin (BSA)-mediated fusion sy...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290343
更新日期：2005-09-01 00:00:00
abstract：:Macrophages are important innate immune cells with functions in tissue repair and remodeling, induction and resolution of inflammation, as well as elimination of invading pathogens. In a recent study, Schmidt and colleagues describe the open epigenetic landscape of the human inflammatory macrophages, and the transcrip...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2016.5
更新日期：2016-02-01 00:00:00
abstract：:Leptin is the protein product encoded by the obese (ob) gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic, and diabetic. Since the cloning of leptin in 1994, our knowledge in body ...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/sj.cr.7290038
更新日期：2000-06-01 00:00:00
abstract：:We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspension-cultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system ...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290008
更新日期：1999-06-01 00:00:00
abstract：:In mammals, the inheritance of mitochondrion and its DNA (mtDNA) is strictly maternal, despite the fact that a sperm can inject up to 100 functional mitochondria into the oocyte during fertilization. The mechanisms responsible for the elimination of the paternal mitochondria remain largely unknown. We report here that...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.182
更新日期：2011-12-01 00:00:00
abstract：:The MyD88-independent pathway, one of the two crucial TLR signaling routes, is thought to be a vertebrate innovation. However, a novel Toll/interleukin-1 receptor (TIR) adaptor, designated bbtTICAM, which was identified in the basal chordate amphioxus, links this pathway to invertebrates. The protein architecture of b...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.156
更新日期：2011-10-01 00:00:00
abstract：:In mice treated with antibiotics to deplete commensal microbiota, there is a significant overhaul of host cellular disposition and function with CX3CR1+ mononuclear phagocytic cells carrying pathogenic and non-pathogenic administered bacteria to the (messenteric lymph node) MLN, resulting in T cell stimulation and IgA...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2013.41
更新日期：2013-05-01 00:00:00
abstract：:The Beclin 1 gene is a haplo-insufficient tumor suppressor and plays an essential role in autophagy. However, the molecular mechanism by which Beclin 1 functions remains largely unknown. Here we report the crystal structure of the evolutionarily conserved domain (ECD) of Beclin 1 at 1.6 Å resolution. Beclin 1 ECD exhi...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2012.24
更新日期：2012-03-01 00:00:00
abstract：:Members of the basic helix-loop-helix (bHLH) gene family play important roles in vertebrate neurogenesis. In this study, confocal microscopy-based fluorescence resonance energy transfer (FRET) is used to monitor bHLH protein-protein interactions under various physiological conditions. Tissue-specific bHLH activators, ...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7310076
更新日期：2006-06-01 00:00:00
abstract：:The full-length cDNA of Pinellia ternata agglutinin (PTA) was cloned from inflorescences using RACE-PCR. Through comparative analysis of PTA gene (pta) and its deduced amino acid sequence with those of other Araceae species, pta was found to encode a precursor lectin with signal peptide and to have extensive homology ...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290175
更新日期：2003-08-01 00:00:00
abstract：:Stem cells, including both pluripotent stem cells and multipotent somatic stem cells, hold great potential for interrogating the mechanisms of tissue development, homeostasis and pathology, and for treating numerous devastating diseases. Establishment of in vitro platforms to faithfully maintain and precisely manipula...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/cr.2012.182
更新日期：2013-01-01 00:00:00
abstract：:Multipotent neural stem/progenitor cells hold great promise for cell therapy. The reprogramming of fibroblasts to induced pluripotent stem cells as well as mature neurons suggests a possibility to convert a terminally differentiated somatic cell into a multipotent state without first establishing pluripotency. Here, w...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.175
更新日期：2012-01-01 00:00:00
abstract：:Apoptosis is a form of genetically programmed cell death, which plays a key role in regulation of cellularity in a variety of tissue and cell types including the cardiovascular tissues. Under both physiological and pathophysiological conditions, various biophysiological and biochemical factors, including mechanical fo...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/sj.cr.7290094
更新日期：2001-12-01 00:00:00
abstract：:Transcriptional activator-like effectors (TALEs) have emerged as powerful tools for genome editing. A recent study published by Cell Research reports that fusion of thioredoxin to TALEs unlocks their full potential in live-cell imaging to accurately analyze genome instability, telomere attrition and epigenetic alterat...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2017.33
更新日期：2017-04-01 00:00:00
abstract：:Platelets play an essential role in hemostasis and atherothrombosis. Owing to their short storage time, there is constant demand for this life-saving blood component. In this study, we report that it is feasible to generate functional megakaryocytes and platelets from human embryonic stem cells (hESCs) on a large scal...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.8
更新日期：2011-03-01 00:00:00
abstract：:Cytosine methylation of genomic DNA controls gene expression and maintains genome stability. How a specific DNA sequence is targeted for methylation by a methyltransferase is largely unknown. Here, we show that histone H3 tails lacking lysine 4 (K4) methylation function as an allosteric activator for methyltransferase...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2011.92
更新日期：2011-08-01 00:00:00
abstract：:Elimination of misfolded proteins of the endoplasmic reticulum (ER) requires their retrotranslocation from the ER to the cytosol via membrane-bound ubiquitin ligase complexes. Baldridge and Rapoport now reconstitute a key step of retrotranslocation, demonstrating a protein conduit gated by ubiquitination. ...
journal_title：Cell research
pub_type： 评论,杂志文章
doi：10.1038/cr.2016.92
更新日期：2016-10-01 00:00:00
abstract：:The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. For obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investi...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.1997.4
更新日期：1997-06-01 00:00:00
abstract：:Mitogen-activated protein kinase (MAPK) cascades play important roles in regulating plant innate immune responses. In a genetic screen to search for mutants with constitutive defense responses, we identified multiple alleles of mpk4 and mekk1 that exhibit cell death and constitutive defense responses. Bimolecular fluo...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/cr.2008.300
更新日期：2008-12-01 00:00:00
abstract：:Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray...
journal_title：Cell research
pub_type： 杂志文章
doi：10.1038/sj.cr.7290151
更新日期：2003-02-01 00:00:00
abstract：:The family members of the mitogen-activated protein (MAP) kinases mediate a wide variety of cellular behaviors in response to extracellular stimuli. One of the four main sub-groups, the p38 group of MAP kinases, serve as a nexus for signal transduction and play a vital role in numerous biological processes. In this re...
journal_title：Cell research
pub_type： 杂志文章,评审
doi：10.1038/sj.cr.7290257
更新日期：2005-01-01 00:00:00
