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Complementary recognition in condensed DNA: accelerated DNA renaturation.

Abstract：

:The functional consequences of DNA condensation are investigated. The recognition of complementary strands is profoundly modified by this critical phenomenon. (1) Condensation of denatured DNA greatly accelerates the kinetics of DNA renaturation. We propose a unifying explanation for the effects of several accelerating solvents studied here including polymers, di- and multivalent cations, as well as effects seen with the phenol emulsions and single-stranded nucleic acid binding proteins. Optimal conditions for renaturation at or above the calculated three dimensional diffusion limit are theoretically consistent with a limited search space in the condensed phases. (2) In addition to these effects on association of two single strands, similar condensation acceleration effects can be seen in strand exchange experiments with double stranded DNA without proteins. These may model a mechanism of recombinational protein function.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Sikorav JL,Church GM

doi

10.1016/0022-2836(91)90595-w

subject

Has Abstract

pub_date

1991-12-20 00:00:00

pages

1085-108

issue

4

eissn

0022-2836

issn

1089-8638

pii

0022-2836(91)90595-W

journal_volume

222

pub_type

杂志文章

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