Electroolfactogram responses from organotypic cultures of the olfactory epithelium from postnatal mice.

Abstract:

:Organotypic cultures of the mouse olfactory epithelium connected to the olfactory bulb were obtained with the roller tube technique from postnatal mice aged between 13 and 66 days. To test the functionality of the cultures, we measured electroolfactograms (EOGs) at different days in vitro (DIV), up to 7 DIV, and we compared them with EOGs from identical acute preparations (0 DIV). Average amplitudes of EOG responses to 2 mixtures of various odorants at concentrations of 1 mM or 100 microM decreased in cultures between 2 and 5 DIV compared with 0 DIV. The percentage of responsive cultures was 57%. We also used the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) to trigger the olfactory transduction cascade bypassing odorant receptor activation. Average amplitudes of EOG responses to 500 microM IBMX were not significantly different in cultures up to 6 DIV or 0 DIV, and the average percentage of responsive cultures between 2 and 5 DIV was 72%. The dose-response curve to IBMX measured in cultures up to 7 DIV was similar to that at 0 DIV. Moreover, the percentage of EOG response to IBMX blocked by niflumic acid, a blocker of Ca-activated Cl channels, was not significantly different in cultured or acute preparations.

journal_name

Chem Senses

journal_title

Chemical senses

authors

Pinato G,Rievaj J,Pifferi S,Dibattista M,Masten L,Menini A

doi

10.1093/chemse/bjn007

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

397-404

issue

4

eissn

0379-864X

issn

1464-3553

pii

bjn007

journal_volume

33

pub_type

杂志文章
  • Derivation and evaluation of a labeled hedonic scale.

    abstract::The objective of this study was to develop a semantically labeled hedonic scale (LHS) that would yield ratio-level data on the magnitude of liking/disliking of sensation equivalent to that produced by magnitude estimation (ME). The LHS was constructed by having 49 subjects who were trained in ME rate the semantic magn...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjp054

    authors: Lim J,Wood A,Green BG

    更新日期:2009-11-01 00:00:00

  • Cross-adaptation and bitterness inhibition of L-tryptophan, L-phenylalanine and urea: further support for shared peripheral physiology.

    abstract::A previous study investigating individuals' bitterness sensitivities found a close association among three compounds: L-tryptophan (L-trp), L-phenylalanine (L-phe) and urea (Delwiche et al., 2001, Percept. Psychophys. 63, 761-776). In the present experiment, psychophysical cross-adaptation and bitterness inhibition ex...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/27.2.123

    authors: Keast RS,Breslin PA

    更新日期:2002-02-01 00:00:00

  • Temporal integration in nasal lateralization and nasal detection of carbon dioxide.

    abstract::Two experiments examined time/concentration trading for the detection of carbon dioxide, an irritant with little or no odor. Experiment 1 employed the nasal lateralization method: subjects attempted to determine which nostril received carbon dioxide and which received pure air when presented simultaneously. Experiment...

    journal_title:Chemical senses

    pub_type: 临床试验,杂志文章

    doi:10.1093/chemse/bjh018

    authors: Wise PM,Radil T,Wysocki CJ

    更新日期:2004-02-01 00:00:00

  • A Neural Mechanism of Cue-Outcome Expectancy Generated by the Interaction Between Orbitofrontal Cortex and Amygdala.

    abstract::Taste perception is important for animals to take adequate nutrients and avoid toxins for their survival. Appetitive and aversive behaviors are produced by value evaluation of taste and taste expectation caused by other sensations. The value evaluation, coupled with a cue presentation, produces outcome expectation and...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjz066

    authors: Takei K,Fujita K,Kashimori Y

    更新日期:2020-01-01 00:00:00

  • Automated analysis of breathing waveforms using BreathMetrics: a respiratory signal processing toolbox.

    abstract::Nasal inhalation is the basis of olfactory perception and drives neural activity in olfactory and limbic brain regions. Therefore, our ability to investigate the neural underpinnings of olfaction and respiration can only be as good as our ability to characterize features of respiratory behavior. However, recordings of...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjy045

    authors: Noto T,Zhou G,Schuele S,Templer J,Zelano C

    更新日期:2018-09-22 00:00:00

  • The origin of slow potentials on the tongue surface induced by frog glossopharyngeal efferent fiber stimulation.

    abstract::When the glossopharyngeal (GP) nerve of the frog was stimulated electrically, electropositive slow potentials were recorded from the tongue surface and depolarizing slow potentials from taste cells in the fungiform papillae. The amplitude of the slow potentials was stimulus strength- and the frequency-dependent. Gener...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/25.5.583

    authors: Sato T,Toda K,Miyamoto T,Okada Y,Fujiyama R

    更新日期:2000-10-01 00:00:00

  • Human responses to propionic acid. II. Quantification of breathing responses and their relationship to perception.

    abstract::In 20 normal and four anosmic participants, instantaneous inhalation and exhalation flow rates were recorded in response to 15 s stimulations with clean air or propionic acid concentrations (0.16, 1.14, 8.22 and 59.15 p.p.m., v/v) that ranged from peri-threshold for normals to clearly supra-threshold for anosmics. Eac...

    journal_title:Chemical senses

    pub_type: 临床试验,杂志文章

    doi:10.1093/chemse/26.4.351

    authors: Walker JC,Kendal-Reed M,Hall SB,Morgan WT,Polyakov VV,Lutz RW

    更新日期:2001-05-01 00:00:00

  • Alarm pheromone that aggravates stress-induced hyperthermia is soluble in water.

    abstract::We previously reported that stressed male Wistar rats released alarm pheromone from the perianal region, which aggravated stress-induced hyperthermia and increased Fos expression in the mitral/tufted cell layer of the accessory olfactory bulb in recipient rats. In this study, we attempted to obtain this pheromone in w...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bji044

    authors: Kiyokawa Y,Kikusui T,Takeuchi Y,Mori Y

    更新日期:2005-07-01 00:00:00

  • Measurement of sensitivity to olfactory flavor: application in a study of aging and dentures.

    abstract::Olfaction involves a dual sensory process for perceiving odors orthonasally (through the nostrils) and retronasally (through the mouth). This investigation entailed developing a measure of sensitivity to an odor delivered in an orally sampled food (orange flavoring in a sucrose-sweetened gelatin) and examining sensiti...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/24.6.671

    authors: Duffy VB,Cain WS,Ferris AM

    更新日期:1999-12-01 00:00:00

  • Using Errors by Guard Honeybees (Apis mellifera) to Gain New Insights into Nestmate Recognition Signals.

    abstract::Although the honeybee (Apis mellifera) is one of the world most studied insects, the chemical compounds used in nestmate recognition, remains an open question. By exploiting the error prone recognition system of the honeybee, coupled with genotyping, we studied the correlation between cuticular hydrocarbon (CHC) profi...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjv053

    authors: Pradella D,Martin SJ,Dani FR

    更新日期:2015-11-01 00:00:00

  • A sexually dimorphic group of atypical glomeruli in the mouse olfactory bulb.

    abstract::Atypical glomeruli (AtG) are clearly distinguishable from typical ones because of their strong cholinergic innervation. AtG are located in defined positions in the caudal half of the main olfactory bulb of rodents. The AtG partially overlap with other specialized olfactory subsystems, such as the modified glomerular c...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/26.1.7

    authors: Weruaga E,Briñón JG,Porteros A,Arévalo R,Aijón J,Alonso JR

    更新日期:2001-01-01 00:00:00

  • Do tastants have a smell?

    abstract::The stimuli used in taste research are usually considered to be odourless. This was tested in two experiments with aqueous solutions of two representative compounds for each of the five taste qualities including umami. In the first experiment elderly and young subjects rated the intensity and pleasantness of three con...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjh251

    authors: Mojet J,Köster EP,Prinz JF

    更新日期:2005-01-01 00:00:00

  • Psychometrics of odor quality discrimination: method for threshold determination.

    abstract::There is no natural physical continuum for odor quality along which an odor quality discrimination (OQD) threshold can be measured. In an attempt to overcome this problem, the substitution-reciprocity (SURE) method defines a framework for the measurement of an OQD threshold. More specifically, it (i) defines a thresho...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/25.5.493

    authors: Olsson MJ,Cain WS

    更新日期:2000-10-01 00:00:00

  • Decreased perception of bourgeonal may be linked to male idiopathic infertility.

    abstract::Regarding the chemotaxis of sperms, new insights have been gained during the last 20 years. Olfactory receptors are expressed on the flagellar midpiece of human spermatogenic cells. One of them, OR1D2, is also expressed in the olfactory epithelium. This receptor has been suggested to play a role in sperm chemotaxis an...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjt009

    authors: Sinding C,Kemper E,Spornraft-Ragaller P,Hummel T

    更新日期:2013-06-01 00:00:00

  • Calcium signaling in taste cells: regulation required.

    abstract::Peripheral taste receptor cells depend on distinct calcium signals to generate appropriate cellular responses that relay taste information to the central nervous system. Some taste cells have conventional chemical synapses and rely on calcium influx through voltage-gated calcium channels. Other taste cells lack these ...

    journal_title:Chemical senses

    pub_type: 杂志文章,评审

    doi:10.1093/chemse/bjq082

    authors: Medler KF

    更新日期:2010-11-01 00:00:00

  • Histochemical identification of carbohydrate moieties in the accessory olfactory bulb of the mouse using a panel of lectins.

    abstract::Lectin binding patterns in the olfactory bulb of the mouse were investigated using 12 biotinylated lectins. Three, with specificities for galactose, N-acetylgalactosamine and L-fucose, stained only the nervous and glomerular layers of the accessory olfactory bulb; four, with specificities for galactose or N-acetylgluc...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/26.6.645

    authors: Salazar I,Sánchez Quinteiro P,Lombardero M,Cifuentes JM

    更新日期:2001-07-01 00:00:00

  • Emotional Body Odors as Context: Effects on Cardiac and Subjective Responses.

    abstract::Many studies have indicated that the chemical cues from body odors (BOs) of donors experiencing negative emotions can influence the psychophysiological and behavioral response of the observers. However, these olfactory cues have been used mainly as contextual information for processing visual stimuli. Here, for the fi...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjy021

    authors: Ferreira J,Parma V,Alho L,Silva CF,Soares SC

    更新日期:2018-05-23 00:00:00

  • Taste solution preferences of C57BL/6J and 129X1/SvJ mice: influence of age, sex, and diet.

    abstract::To examine whether age influences taste solution preferences, we measured taste preferences of C57BL/6J and 129X1/SvJ mice given a series of 48-h 2-bottle tests with a choice between water and one of the following taste solutions: 2 mM saccharin, 5 mM citric acid, 30 microM quinine hydrochloride, 75 mM sodium chloride...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm034

    authors: Tordoff MG

    更新日期:2007-09-01 00:00:00

  • Oral Digestion and Perception of Starch: Effects of Cooking, Tasting Time, and Salivary α-Amylase Activity.

    abstract::Since starch is a significant part of human diet, its oral detection would be highly beneficial. This study was designed to determine whether starch or its degradation products can be tasted and what factors influence its perception. Subjects were asked 1) to taste 8% raw and cooked starch samples for 5, 15, and 35 s ...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjx042

    authors: Lapis TJ,Penner MH,Balto AS,Lim J

    更新日期:2017-10-01 00:00:00

  • Olfactory functions in Parkinson's disease and Alzheimer's disease.

    abstract::The aim of this investigation was to compare olfactory functions of patients suffering from Parkinson's disease (PD) and Alzheimer's disease (AD). Olfactory threshold, odor identification ability and odor memory performance were assessed in 21 non-demented PD patients and in 22 AD patients. Both patient groups were im...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/22.1.105

    authors: Lehrner JP,Brücke T,Dal-Bianco P,Gatterer G,Kryspin-Exner I

    更新日期:1997-02-01 00:00:00

  • Expression of trace amine-associated receptors in the Grueneberg ganglion.

    abstract::The Grueneberg ganglion (GG) in the vestibule of the anterior nasal cavity is considered as an olfactory subcompartment based on expression of the olfactory marker protein (OMP) and axonal projection to the olfactory bulb. Searching for olfactory receptors present in the GG, it has been observed recently that V2r83, a...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm032

    authors: Fleischer J,Schwarzenbacher K,Breer H

    更新日期:2007-07-01 00:00:00

  • The influence of sodium salts on binary mixtures of bitter-tasting compounds.

    abstract::In order to study potential mixture interactions among bitter compounds, selected sodium salts were added to five compounds presented either alone or as binary bitter-compound mixtures. Each compound was tested at a concentration that elicited 'weak' perceived bitterness. The bitter compounds were mixed at these conce...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjh045

    authors: Keast RS,Canty TM,Breslin PA

    更新日期:2004-06-01 00:00:00

  • Female snake sex pheromone induces membrane responses in vomeronasal sensory neurons of male snakes.

    abstract::The vomeronasal organ (VNO) is important for activating accessory olfactory pathways that are involved in sexually dimorphic mating behavior. The VNO of male garter snakes is critically important for detection of, and response to, female sex pheromones. In the present study, under voltage-clamp conditions, male snake ...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjj056

    authors: Huang GZ,Zhang JJ,Wang D,Mason RT,Halpern M

    更新日期:2006-07-01 00:00:00

  • Response accuracy and odor sampling time in mice trained to discriminate between enantiomers of carvone and those of terpinen-4-ol.

    abstract::Response accuracy and odor-sampling times were used to compare the ability of mice to detect (+)-carvone and (+)-terpinen-4-ol and to discriminate between enantiomers of carvone and of terpinen-4-ol. Except for increased odor sampling when mice were first exposed to the (+)-carvone odor, there was no difference in odo...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm039

    authors: Slotnick B

    更新日期:2007-09-01 00:00:00

  • Sex-specific responses to urinary chemicals by the mouse vomeronasal organ.

    abstract::Social behaviors of most mammals are affected by chemical signals, pheromones, exchanged between conspecifics. Previous experiments have shown that behavioral responses to the same pheromone differ depending on the sex and endocrine status of the respondent. Although the exact mechanism of this dimorphism is not known...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjh076

    authors: Thompson RN,Robertson BK,Napier A,Wekesa KS

    更新日期:2004-11-01 00:00:00

  • Ca2+ stabilizes the membrane potential of moth olfactory receptor neurons at rest and is essential for their fast repolarization.

    abstract::The role of Ca(2+) in insect olfactory transduction was studied in the moth Spodoptera littoralis. Single sensillum recordings were made to investigate in vivo the role of sensillar Ca(2+) on the electrophysiological properties of sex pheromone responsive olfactory receptor neurons (ORNs). Lowering the sensillar Ca(2+...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjl059

    authors: Pézier A,Acquistapace A,Renou M,Rospars JP,Lucas P

    更新日期:2007-05-01 00:00:00

  • Depression of gustatory receptor potential in frog taste cell by parasympathetic nerve-induced slow hyperpolarizing potential.

    abstract::Parasympathetic nerve (PSN) innervates taste cells of the frog taste disk, and electrical stimulation of PSN elicited a slow hyperpolarizing potential (HP) in taste cells. Here we report that gustatory receptor potentials in frog taste cells are depressed by PSN-induced slow HPs. When PSN was stimulated at 30 Hz durin...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjl028

    authors: Sato T,Nishishita K,Mineda T,Okada Y,Toda K

    更新日期:2007-01-01 00:00:00

  • Food perception with age and its relationship to pleasantness.

    abstract::Differences between elderly subjects (n = 46, 61-86 years) and young subjects (n = 36, 18-25 years) in food perception and food liking were investigated. Intensity and liking ratings were assessed for custard dessert, in which flavor enrichment, textural change, and irritant addition were incorporated as strategies to...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjm028

    authors: Kremer S,Bult JH,Mojet J,Kroeze JH

    更新日期:2007-07-01 00:00:00

  • Expression patterns of odorant receptors and response properties of olfactory sensory neurons in aged mice.

    abstract::The sense of smell deteriorates in normal aging, but the underling mechanisms are still elusive. Here we investigated age-related alterations in expression patterns of odorant receptor (OR) genes and functional properties of olfactory sensory neurons (OSNs)-2 critical factors that define the odor detection threshold i...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/bjp056

    authors: Lee AC,Tian H,Grosmaitre X,Ma M

    更新日期:2009-10-01 00:00:00

  • Electrophysiological properties of frog olfactory supporting cells.

    abstract::Cells, identified as supporting cells by Lucifer Yellow injection, were recorded from slices of frog olfactory epithelium using patch-clamp recordings. Cell-attached single-channel recordings indicated that the intracellular potential (IP) was -68 +/- 7 mV (n = 22) with 4 mM K+ in the bath ([K+]o). IP was -67 +/- 4 mV...

    journal_title:Chemical senses

    pub_type: 杂志文章

    doi:10.1093/chemse/23.3.363

    authors: Trotier D

    更新日期:1998-06-01 00:00:00