Abstract：
:Cytosine DNA methylation is important in regulating gene expression and in silencing transposons and other repetitive sequences. Recent genomic studies in Arabidopsis thaliana have revealed that many endogenous genes are methylated either within their promoters or within their transcribed regions, and that gene methylation is highly correlated with transcription levels. However, plants have different types of methylation controlled by different genetic pathways, and detailed information on the methylation status of each cytosine in any given genome is lacking. To this end, we generated a map at single-base-pair resolution of methylated cytosines for Arabidopsis, by combining bisulphite treatment of genomic DNA with ultra-high-throughput sequencing using the Illumina 1G Genome Analyser and Solexa sequencing technology. This approach, termed BS-Seq, unlike previous microarray-based methods, allows one to sensitively measure cytosine methylation on a genome-wide scale within specific sequence contexts. Here we describe methylation on previously inaccessible components of the genome and analyse the DNA methylation sequence composition and distribution. We also describe the effect of various DNA methylation mutants on genome-wide methylation patterns, and demonstrate that our newly developed library construction and computational methods can be applied to large genomes such as that of mouse.
journal_name
Naturejournal_title
Natureauthors
Cokus SJ,Feng S,Zhang X,Chen Z,Merriman B,Haudenschild CD,Pradhan S,Nelson SF,Pellegrini M,Jacobsen SEdoi
10.1038/nature06745subject
Has Abstractpub_date
2008-03-13 00:00:00pages
215-9issue
7184eissn
0028-0836issn
1476-4687pii
nature06745journal_volume
452pub_type
杂志文章相关文献
NATURE文献大全abstract：:Hepatocellularcarcinoma (HCC) that occur in woodchucks chronically infected with woodchuck hepatitis virus (WHV) were screened for activation of cellular oncogenes. Enhanced expression and allelic alterations of the c-myc oncogene were found in three HCC out of nine. Variations in the size of the c-myc transcripts, ra...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/324276a0
更新日期：1986-11-20 00:00:00
abstract：:Mice of the C57BL/6 strain are resistant to the development of skin squamous carcinomas (SCCs) induced by an activated Ras oncogene, whereas FVB/N mice are highly susceptible. The genetic basis of this difference in phenotype is unknown. Here we show that susceptibility to SCC is under the control of a carboxy-termina...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature05489
更新日期：2007-02-15 00:00:00
abstract：:The genetic relationships of human populations have been studied by comparing gene frequency data for protein and blood-group loci of different populations. DNA analysis now promises to be more informative since not only do the DNA coding sequences have more variation than their corresponding proteins but, in addition...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/319491a0
更新日期：1986-02-06 00:00:00
abstract：:Inositol 1,4,5-trisphosphate (InsP3) can initiate calcium release into the cytoplasm in a variety of cells. From experiments using permeabilized cells, membrane vesicles, and patch-clamp techniques, it has been suggested that InsP3 acts by directly opening calcium channels. Here, we show that InsP3 induced openings of...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/336583a0
更新日期：1988-12-08 00:00:00
abstract：:Early observations indicated that the Earth's Van Allen radiation belts could be separated into an inner zone dominated by high-energy protons and an outer zone dominated by high-energy electrons. Subsequent studies showed that electrons of moderate energy (less than about one megaelectronvolt) often populate both zon...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13956
更新日期：2014-11-27 00:00:00
abstract：:Interleukin (IL)-2 is a pleiotropic cytokine that is necessary to prevent chronic inflammation in the gastrointestinal tract1-4. The protective effects of IL-2 involve the generation, maintenance and function of regulatory T (Treg) cells4-8, and the use of low doses of IL-2 has emerged as a potential therapeutic strat...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1082-x
更新日期：2019-04-01 00:00:00
abstract：:The amplitude and frequency of laser light can be routinely measured and controlled on a femtosecond (10(-15) s) timescale. However, in pulses comprising just a few wave cycles, the amplitude envelope and carrier frequency are not sufficient to characterize and control laser radiation, because evolution of the light f...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01414
更新日期：2003-02-06 00:00:00
abstract：:Establishment and maintenance of proper architecture is essential for endoplasmic reticulum (ER) function. Homotypic membrane fusion is required for ER biogenesis and maintenance, and has been shown to depend on GTP hydrolysis. Here we demonstrate that Drosophila Atlastin--the fly homologue of the mammalian GTPase atl...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature08280
更新日期：2009-08-20 00:00:00
abstract：:p97 is a 97,000 molecular weight cell-surface glycoprotein, which is present in human melanomas but in only trace amounts in normal adult tissues. Amino acid sequence and iron binding studies have shown that p97 is structurally and functionally related to transferrin. Reports that the genes for the transferrin recepto...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/303070a0
更新日期：1983-05-05 00:00:00
abstract：:The recent discoveries of RNA interference and related RNA silencing pathways have revolutionized our understanding of gene regulation. RNA interference has been used as a research tool to control the expression of specific genes in numerous experimental organisms and has potential as a therapeutic strategy to reduce ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature02872
更新日期：2004-09-16 00:00:00
abstract：:During puberty, the mouse mammary gland develops into a highly branched epithelial network. Owing to the absence of exclusive stem cell markers, the location, multiplicity, dynamics and fate of mammary stem cells (MaSCs), which drive branching morphogenesis, are unknown. Here we show that morphogenesis is driven by pr...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature21046
更新日期：2017-02-16 00:00:00
abstract：:Electrons in a single sheet of graphene behave quite differently from those in traditional two-dimensional electron systems. Like massless relativistic particles, they have linear dispersion and chiral eigenstates. Furthermore, two sets of electrons centred at different points in reciprocal space ('valleys') have this...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature09330
更新日期：2010-09-09 00:00:00
abstract：:Common genetic contributions to autism spectrum disorder (ASD) reside in risk gene variants that individually have minimal effect sizes. As environmental factors that perturb neurodevelopment also underlie idiopathic ASD, it is crucial to identify altered regulators that can orchestrate multiple ASD risk genes during ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-018-0423-5
更新日期：2018-08-01 00:00:00
abstract：:Although it is well established that family members resemble each other in intelligence , the extent to which this results from either shared genes or a shared environment remains controversial, perhaps especially since the relevant evidence presented by Burt has been shown probably to have been fabricated. The influe...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/309620a0
更新日期：1984-06-14 00:00:00
abstract：:The retina in most mammals contains two types of macroglial cells--Müller cells, which span the entire thickness of the retina, and astrocytes, which are mainly confined to the nerve fibre layer. Whereas Müller cells are diffusely distributed in all vertebrate retinae, the presence and distribution of retinal astrocyt...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/332834a0
更新日期：1988-04-28 00:00:00
abstract：:Measurement of the free calcium concentration within a photo-receptor outer segment has been considered an important aim since the proposal by Hagins and Yoshikami that the primary event in phototransduction is a release of Ca (2+) inside the cell. More recent evidence has cast doubt on the calcium hypothesis, and the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/322261a0
更新日期：1986-07-17 00:00:00
abstract：:Hunter-Schreger bands (HSB) are seen in teeth that are composed of crossed sets of enamel prisms. They are present in the teeth of man and many other mammals, but absent in most insectivores and multituberculates. It has been suggested that the presence of HSB makes the tooth enamel less likely to split and is associa...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/328150a0
更新日期：1987-07-09 00:00:00
abstract：:Most of the progress in human culture has required the exploitation of energy resources. About 100 years ago, the major source of energy shifted from recent solar to fossil hydrocarbons, including liquid and gaseous petroleum. Technology has generally led to a greater use of hydrocarbon fuels for most human activities...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02130
更新日期：2003-11-20 00:00:00
abstract：:The import of nuclear proteins proceeds through the nuclear pore complex and requires nuclear localization signals (NLSs), energy and soluble factors, namely importin-alpha (M(r) 60K), importin-beta (90K) and Ran. Importin-alpha is primarily responsible for NLS recognition and is a member of a protein family that incl...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/377246a0
更新日期：1995-09-21 00:00:00
abstract：:Recurring outbursts associated with matter flowing onto compact stellar remnants (such as black holes, neutron stars and white dwarfs) in close binary systems provide a way of constraining the poorly understood accretion process. The light curves of these outbursts are shaped by the efficiency of angular-momentum (and...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature25159
更新日期：2018-02-01 00:00:00
abstract：:Wild-type p53 protein has many properties consistent with its being the product of a tumour suppressor gene. Although the normal roles of tumour suppressor genes are still largely unknown, it seems that they could be involved in promoting cell differentiation as well as in mediating growth arrest by growth-inhibitory ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/352345a0
更新日期：1991-07-25 00:00:00
abstract：:Truncation of the tumour suppressor adenomatous polyposis coli (Apc) constitutively activates the Wnt/beta-catenin signalling pathway. Apc has a role in development: for example, embryos of mice with truncated Apc do not complete gastrulation. To understand this role more fully, we examined the effect of truncated Apc...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02028
更新日期：2003-10-09 00:00:00
abstract：:Mutations in the Schizosaccharomyces pombe cdc8 gene impair cytokinesis. Here we clone cdc8+ and find that it encodes a novel tropomyosin. Gene disruption results in lethal arrest of the cell cycle, but spore germination, cell growth, DNA replication and mitosis are all unaffected. Haploid cdc8 gene disruptants are re...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/360084a0
更新日期：1992-11-05 00:00:00
abstract：:Lipid-bilayer membranes supported on solid substrates are widely used as cell-surface models that connect biological and artificial materials. They can be placed either directly on solids or on ultrathin polymer supports that mimic the generic role of the extracellular matrix. The tools of modern genetic engineering a...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature04164
更新日期：2005-09-29 00:00:00
abstract：:Migration of cells in higher organisms is mediated by adhesion receptors, such as integrins, that link the cell to extracellular-matrix ligands, transmitting forces and signals necessary for locomotion. Whether cells will migrate or not on a given substratum, and also their speed, depends on several variables related ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/385537a0
更新日期：1997-02-06 00:00:00
abstract：:The tyrosine phosphatase SHP2, encoded by PTPN11, is required for the survival, proliferation and differentiation of various cell types. Germline activating mutations in PTPN11 cause Noonan syndrome, whereas somatic PTPN11 mutations cause childhood myeloproliferative disease and contribute to some solid tumours. Recen...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12396
更新日期：2013-07-25 00:00:00
abstract：:Modification of tyrosine residues of proteins has attracted great interest. Although phosphorylation has received most attention, tyrosine-O-sulphation is also common and has been closely investigated in the hormones gastrin and cholecystokinin (CCK). CCK regulates pancreatic enzyme secretion. It is always sulphated a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/309456a0
更新日期：1984-05-31 00:00:00
abstract：:A putative growth hormone receptor from rabbit liver and the growth hormone binding protein from rabbit serum have the same amino-terminal amino-acid sequence, indicating that the binding protein corresponds to the extracellular hormone-binding domain of the liver receptor. The complete amino-acid sequences derived fr...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/330537a0
更新日期：1987-12-10 00:00:00
abstract：:The specification of both the germ line and abdomen in Drosophila depends on the localization of oskar messenger RNA to the posterior of the oocyte. This localization requires several trans-acting factors, including Barentsz and the Mago-Y14 heterodimer, which assemble with oskar mRNA into ribonucleoprotein particles ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature02351
更新日期：2004-02-19 00:00:00
abstract：:The most recent Ebola virus outbreak in West Africa, which was unprecedented in the number of cases and fatalities, geographic distribution, and number of nations affected, highlights the need for safe, effective, and readily available antiviral agents for treatment and prevention of acute Ebola virus (EBOV) disease (...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature17180
更新日期：2016-03-17 00:00:00
