Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method.

Abstract:

BACKGROUND:Flow cytometry allows specific assessment of the expression of ZAP-70, a promising new prognostic factor in B-cell chronic lymphocytic leukemia (B-CLL), but suffers from a lack of multicenter standardization. DESIGN AND METHODS:An optimized method for direct detection of ZAP-70 in flow cytometry was tested in a multicenter fashion. Adapted for frozen cells, this method includes a normalization step by addition of B cells from a pool of peripheral blood mononuclear cells collected from normal donors. ZAP-70 expression levels were assessed for 153 patients with typical B-cell chronic lymphocytic leukemia chronic lymphocytic leukemia. Results were expressed as the ratio of ZAP-70 mean fluorescence intensity between B-CLL cells and normal B cells. RESULTS:The statistically optimized cut-off of ZAP-70 positivity was a ratio of 1.4. Concordance between ZAP-70 and CD38 expression was 67%. Concordance between the mutational status of IgVH genes and ZAP-70 or CD38 expression was 87% and 65%, respectively. ZAP-70 was significantly expressed in 28%, 54% and 61% of patients with Binet stages A, B and C B-cell chronic lymphocytic leukemia, respectively (p=0.008). The absence of ZAP-70 expression was associated with isolated del(13q14), a cytogenetic abnormality with a good prognosis, while most patients with the del(17p13) poor prognosis cytogenetic marker expressed ZAP-70 (p<10(-5)). ZAP-70 expression was not related to the other poor prognosis cytogenetic abnormality del(11q22.3) nor to trisomy 12. CONCLUSIONS:This new technique provides highly reliable results well correlated with the mutational status of IgVH genes, CD38 expression, Binet stage and cytogenetic abnormalities. This robust discriminative technique appears of particular interest for routine diagnosis and assessment of ZAP-70 expression in large, prospective, multicenter therapeutic trials.

journal_name

Haematologica

journal_title

Haematologica

authors

Gachard N,Salviat A,Boutet C,Arnoulet C,Durrieu F,Lenormand B,Leprêtre S,Olschwang S,Jardin F,Lafage-Pochitaloff M,Penther D,Sainty D,Reminieras L,Feuillard J,Béné MC,GEIL.

doi

10.3324/haematol.11622

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

215-23

issue

2

eissn

0390-6078

issn

1592-8721

pii

haematol.11622

journal_volume

93

pub_type

杂志文章,多中心研究
  • Stat5 is critical for the development and maintenance of myeloproliferative neoplasm initiated by Nf1 deficiency.

    abstract::Juvenile myelomonocytic leukemia is a rare myeloproliferative neoplasm characterized by hyperactive RAS signaling. Neurofibromin1 (encoded by the NF1 gene) is a negative regulator of RAS activation. Patients with neurofibromatosis type 1 harbor loss-of-function mutations in NF1 and have a 200- to 500-fold increased ri...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2015.136002

    authors: Sachs Z,Been RA,DeCoursin KJ,Nguyen HT,Mohd Hassan NA,Noble-Orcutt KE,Eckfeldt CE,Pomeroy EJ,Diaz-Flores E,Geurts JL,Diers MD,Hasz DE,Morgan KJ,MacMillan ML,Shannon KM,Largaespada DA,Wiesner SM

    更新日期:2016-10-01 00:00:00

  • From transplant to novel cellular therapies in multiple myeloma: European Myeloma Network guidelines and future perspectives.

    abstract::Survival of myeloma patients has greatly improved with the use of autologous stem cell transplantation and novel agents, such as proteasome inhibitors, immunomodulatory drugs and monoclonal antibodies. Compared to bortezomib- and lenalidomide-based regimens alone, the addition of high-dose melphalan followed by autolo...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:10.3324/haematol.2017.174573

    authors: Gay F,Engelhardt M,Terpos E,Wäsch R,Giaccone L,Auner HW,Caers J,Gramatzki M,van de Donk N,Oliva S,Zamagni E,Garderet L,Straka C,Hajek R,Ludwig H,Einsele H,Dimopoulos M,Boccadoro M,Kröger N,Cavo M,Goldschmidt H,B

    更新日期:2018-02-01 00:00:00

  • STAT3-mediated activation of microRNA cluster 17~92 promotes proliferation and survival of ALK-positive anaplastic large cell lymphoma.

    abstract::Systemic anaplastic large cell lymphoma is a category of T-cell non-Hodgkin's lymphoma which can be further subdivided into two distinct entities (ALK(+) and ALK(-)) based on the presence or absence of ALK gene rearrangements. Among several pathways triggered by ALK signaling, constitutive activation of STAT3 is stric...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2013.088286

    authors: Spaccarotella E,Pellegrino E,Ferracin M,Ferreri C,Cuccuru G,Liu C,Iqbal J,Cantarella D,Taulli R,Provero P,Di Cunto F,Medico E,Negrini M,Chan WC,Inghirami G,Piva R

    更新日期:2014-01-01 00:00:00

  • Heme controls ferroportin1 (FPN1) transcription involving Bach1, Nrf2 and a MARE/ARE sequence motif at position -7007 of the FPN1 promoter.

    abstract:BACKGROUND:Macrophages of the reticuloendothelial system play a key role in recycling iron from hemoglobin of senescent or damaged erythrocytes. Heme oxygenase 1 degrades the heme moiety and releases inorganic iron that is stored in ferritin or exported to the plasma via the iron export protein ferroportin. In the plas...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2009.020123

    authors: Marro S,Chiabrando D,Messana E,Stolte J,Turco E,Tolosano E,Muckenthaler MU

    更新日期:2010-08-01 00:00:00

  • The fusion proteins TEL-PDGFRbeta and FIP1L1-PDGFRalpha escape ubiquitination and degradation.

    abstract:BACKGROUND:Chimeric oncogenes encoding constitutively active protein tyrosine kinases are associated with chronic myeloid neoplasms. TEL-PDGFRbeta (TPbeta, also called ETV6-PDGFRB) is a hybrid protein produced by the t(5;12) translocation, FIP1L1-PDGFRalpha (FPalpha) results from a deletion on chromosome 4q12 and ZNF19...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2008.001149

    authors: Toffalini F,Kallin A,Vandenberghe P,Pierre P,Michaux L,Cools J,Demoulin JB

    更新日期:2009-08-01 00:00:00

  • Alcohol-induced pain in tuberculous adenitis.

    abstract::Since its first description in Hodgkin's disease, alcohol-induced pain has subsequently been described both in other neoplasms and in various non-malignant conditions. There seems little doubt that ethyl alcohol itself is responsible, although the mechanism for producing pain remains elusive. We report a case of alcoh...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Custodi P,Cerutti A,Bagnato R,Cassani P

    更新日期:1993-11-01 00:00:00

  • The role of the JAK2 GGCC haplotype and the TET2 gene in familial myeloproliferative neoplasms.

    abstract:BACKGROUND:Myeloproliferative neoplasms constitute a group of diverse chronic myeloid malignancies that share pathogenic features such as acquired mutations in the JAK2, TET2, CBL and MPL genes. There are recent reports that a JAK2 gene haplotype (GGCC or 46/1) confers susceptibility to JAK2 mutation-positive myeloprol...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2010.034488

    authors: Olcaydu D,Rumi E,Harutyunyan A,Passamonti F,Pietra D,Pascutto C,Berg T,Jäger R,Hammond E,Cazzola M,Kralovics R

    更新日期:2011-03-01 00:00:00

  • Somatic hypermutation signature in B-cell low-grade lymphomas.

    abstract:BACKGROUND:Immunoglobulin gene somatic hypermutation is a biologically relevant and clinically useful prognostic factor in different types of low-grade B-cell lymphomas, including chronic lymphocytic leukemia, mantle cell lymphoma and splenic marginal zone lymphoma. DESIGN AND METHODS:With the aim of identifying surro...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.12999

    authors: Tracey L,Aggarwal M,García-Cosio M,Villuendas R,Algara P,Sánchez-Beato M,Sánchez-Aguilera A,García JF,Rodríguez A,Camacho FI,Martínez N,Ruiz-Ballesteros E,Mollejo M,Piris MA

    更新日期:2008-08-01 00:00:00

  • Update on chronic hepatitis C in hemophiliacs.

    abstract:BACKGROUND AND OBJECTIVES:Hepatitis C virus (HCV) infection is an important cause of morbidity and mortality in patients affected by hereditary bleeding disorders and treated with non-virus inactivated clotting factor concentrates during the 1970s. INFORMATION SOURCES:In this review, we briefly report the present know...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:

    authors: Franchini M,Capra F,Tagliaferri A,Rossetti G,De Gironcoli M,Rocca P,Aprili G,Gandini G

    更新日期:2002-05-01 00:00:00

  • Fludarabine and cytarabine as continuous sequential infusion for elderly patients with acute myeloid leukemia.

    abstract:BACKGROUND AND OBJECTIVES:A phase II study was conducted to investigate the effects of a therapeutic program based on the combination of fludarabine and cytarabine (ARA-C) administered as a sequential continuous infusion in untreated elderly patients with acute myeloid leukemia (AML). DESIGN AND METHODS:Sixty-three pa...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Ferrara F,D'Arco AM,De Simone M,Mele G,Califano C,Pocali B,Danise P,Palmieri S

    更新日期:2005-06-01 00:00:00

  • Clinical features and laboratory patterns in a cohort of consecutive Argentinian patients with von Willebrand's disease.

    abstract:BACKGROUND AND OBJECTIVES:von Willebrand's disease (vWD) is a bleeding disorder with variable clinical expression. Our aim was to classify patients with vWD and to determine the phenotype in their relatives. DESIGN AND METHODS:The types and subtypes, blood group frequency and its relevance, bleeding sites, response to...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Woods AI,Meschengieser SS,Blanco AN,Salviú MJ,Farías CE,Kempfer AC,Lazzari MA

    更新日期:2001-04-01 00:00:00

  • Cryoprecipitate versus commercial fibrinogen concentrate in patients who occasionally require a therapeutic supply of fibrinogen: risk comparison in the case of an emerging transfusion-transmitted infection.

    abstract::A probabilistic model was used to compare cryoprecipitate to viral inactivated, commercial fibrinogen concentrate to evaluate with regard to the recipient's risk of exposure to an emergent AIDS-like epidemic. In patients who occasionally need a therapeutic dose of fibrinogen, commercial fibrinogen would be marginally ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.11072

    authors: Pereira A

    更新日期:2007-06-01 00:00:00

  • Allogeneic hematopoietic stem cells from sources other than bone marrow: biological and technical aspects.

    abstract:BACKGROUND AND OBJECTIVE:Identification and characterization of hematopoietic stem cells in peripheral blood (PB) and cord blood (CB) have suggested feasible alternatives to conventional allogeneic bone marrow (BM) transplantation. The growing interest in this use of allogeneic stem cells has prompted the Working Group...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:

    authors: Bertolini F,de Vincentiis A,Lanata L,Lemoli RM,Maccario R,Majolino I,Ponchio L,Rondelli D,Tabilio A,Zanon P,Tura S

    更新日期:1997-03-01 00:00:00

  • Low leukotriene B4 receptor 1 leads to ALOX5 downregulation at diagnosis of chronic myeloid leukemia.

    abstract::ALOX5 is implicated in chronic myeloid leukemia development in mouse leukemic stem cells, but its importance in human chronic myeloid leukemia is unknown. Functional ALOX5 was assessed using an LTB4 ELISA and ALOX5, and LTB4R1 mRNA expression was determined via a TaqMan gene expression assay. LTB4R1 and 5-LOX protein ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2013.101972

    authors: Lucas CM,Harris RJ,Giannoudis A,McDonald E,Clark RE

    更新日期:2014-11-01 00:00:00

  • Peripheral blood or bone marrow cells in reduced-intensity or myeloablative conditioning allogeneic HLA identical sibling donor transplantation for multiple myeloma.

    abstract:BACKGROUND AND OBJECTIVES:Peripheral blood stem cells (PBSC) following reduced intensity conditioning (RIC) are being increasingly used for allogeneic transplantation in multiple myeloma. The purpose of this study was to compare outcome of patients transplanted with either PBSC or bone marrow (BM) following RIC or myel...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.11353

    authors: Gahrton G,Iacobelli S,Bandini G,Björkstrand B,Corradini P,Crawley C,Hegenbart U,Morgan G,Kröger N,Schattenberg A,Schönland SO,Verdonck LF,Volin L,de Witte T,Niederwieser D,Myeloma Subcommittee of the EBMT.

    更新日期:2007-11-01 00:00:00

  • HIV-related thrombotic thrombocytopenic purpura (TTP) as first clinical manifestation of infection.

    abstract::A case of thrombotic thrombocytopenic purpura (TTP) in a 40-year-old bisexual man unaware of being HIV positive is reported. The hematologic syndrome represented the first clinical manifestation of this viral infection. The clinical picture, characterized by severe hemolytic microangiopathic anemia, thrombocytopenia, ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Cruccu V,Parisio E,Pedretti D,Villa A,Confalonieri F

    更新日期:1994-05-01 00:00:00

  • Efficacy of anti-CD20 monoclonal antibodies (Mabthera) in patients with progressed hairy cell leukemia.

    abstract:BACKGROUND AND OBJECTIVES:Recently, a chimeric monoclonal antibody (MoAb) directed against the CD20 antigen (rituximab) has been successfully introduced in the treatment of several CD20-positive B-cell neoplasias and particularly of follicular lymphomas. Based on these premises we evaluated the efficacy and the toxicit...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:

    authors: Lauria F,Lenoci M,Annino L,Raspadori D,Marotta G,Bocchia M,Forconi F,Gentili S,La Manda M,Marconcini S,Tozzi M,Baldini L,Zinzani PL,Foà R

    更新日期:2001-10-01 00:00:00

  • Molecular response assessment by quantitative real-time polymerase chain reaction after induction therapy in NPM1-mutated patients identifies those at high risk of relapse.

    abstract::Monitoring minimal residual disease is an important way to identify patients with acute myeloid leukemia at high risk of relapse. In this study we investigated the prognostic potential of minimal residual disease monitoring by quantitative real-time polymerase chain reaction analysis of NPM1 mutations in patients trea...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:10.3324/haematol.2014.104133

    authors: Hubmann M,Köhnke T,Hoster E,Schneider S,Dufour A,Zellmeier E,Fiegl M,Braess J,Bohlander SK,Subklewe M,Sauerland MC,Berdel WE,Büchner T,Wörmann B,Hiddemann W,Spiekermann K

    更新日期:2014-08-01 00:00:00

  • The status of high-dose chemotherapy with hematopoietic stem cell transplantation in germ cell tumor patients.

    abstract:BACKGROUND AND OBJECTIVES:Germ cell tumors (GCTs) are very chemosensitive cancers, in which high-dose chemotherapy (HDCT) has been investigated as salvage therapy or as first-line treatment in poor prognosis patients. This paper presents an update of available information in order to define the status of HDCT in GCT pa...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:

    authors: De Giorgi U,Rosti G,Papiani G,Marangolo M

    更新日期:2002-01-01 00:00:00

  • Detection of heterozygous large deletions in the antithrombin gene using multiplex polymerase chain reaction and denatured high performance liquid chromatography.

    abstract::The present study reports a method for the easy, rapid and cost effective detection of heterozygous large deletions. As a model gene all exons of the antithrombin gene were amplified in a one tube multiplex polymerase chain reaction (PCR) and the products separated according to their size by reverse-phase ion-pair hig...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Pavlova A,El-Maarri O,Luxembourg B,Lindhoff-Last E,Kochhan L,Bruhn HD,Delev D,Watzka M,Seifried E,Oldenburg J

    更新日期:2006-09-01 00:00:00

  • Relationship between pharmacokinetic profile of subcutaneously administered alemtuzumab and clinical response in patients with chronic lymphocytic leukemia.

    abstract::Alemtuzumab serum levels and clinical response after subcutaneous administration (10 mg 3 times/week for six weeks) have been explored in 29 chronic lymphocytic leukemia patients receiving the monoclonal antibody as consolidation. Serum concentrations after each administration gradually increased during the first week...

    journal_title:Haematologica

    pub_type: 临床试验,杂志文章

    doi:10.3324/haematol.2010.033159

    authors: Montagna M,Montillo M,Avanzini MA,Tinelli C,Tedeschi A,Visai L,Ricci F,Vismara E,Morra E,Regazzi M

    更新日期:2011-06-01 00:00:00

  • The prevalence of extramedullary acute myeloid leukemia detected by 18FDG-PET/CT: final results from the prospective PETAML trial.

    abstract::Extramedullary (EM) disease in patients with acute myeloid leukemia (AML) is a known phenomenon. Since the prevalence of EM AML has so far only been clinically determined on examination, we performed a prospective study in patients with AML. The aim of the study was to determine the prevalence of metabolically active ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2019.223032

    authors: Stölzel F,Lüer T,Löck S,Parmentier S,Kuithan F,Kramer M,Alakel NS,Sockel K,Taube F,Middeke JM,Schetelig J,Röllig C,Paulus T,Kotzerke J,Ehninger G,Bornhäuser M,Schaich M,Zoephel K

    更新日期:2020-06-01 00:00:00

  • Clinical phenotypes and molecular characterization of Hb H-Paksé disease.

    abstract:BACKGROUND AND OBJECTIVES:Hemoglobin Constant Spring (Hb CS), caused by a termination codon mutation (TAA-->CAA) in the a2 gene, is the most common non-deletional type of a thalassemia in Southeast Asia. This mutation can most easily be detected by loss of an MseI-restriction site (T/TAA) spanning the termination codon...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Viprakasit V,Tanphaichitr VS,Pung-Amritt P,Petrarat S,Suwantol L,Fisher C,Higgs DR

    更新日期:2002-02-01 00:00:00

  • Clinical impact of human Jurkat T-cell-line-derived antithymocyte globulin in multiple myeloma patients undergoing allogeneic stem cell transplantation.

    abstract:BACKGROUND:Antithymocyte globulin or human Jurkat T-cell-line-derived antilymphocyte globulin is used in allogeneic stem cell transplantation to induce in vivo T-cell depletion to facilitate engraftment and lower graft-versus-host disease. In vitro studies suggest that antithymocyte globulin, besides causing T-cell dep...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.12665

    authors: Ayuk F,Perez-Simon JA,Shimoni A,Sureda A,Zabelina T,Schwerdtfeger R,Martino R,Sayer HG,Alegre A,Lahuerta JJ,Atanackovic D,Wolschke C,Nagler A,Zander AR,San Miguel JF,Kröger N

    更新日期:2008-09-01 00:00:00

  • Diagnostic approach to lymph node enlargement.

    abstract:BACKGROUND AND OBJECTIVE:How to reach the correct diagnosis of a lymph node enlargement is still a problem which strongly challenges the knowledge and experience of the clinician. Organized and specifically oriented literature on the right sequential steps and the logical criteria that should guide this diagnostic appr...

    journal_title:Haematologica

    pub_type: 杂志文章,评审

    doi:

    authors: Ghirardelli ML,Jemos V,Gobbi PG

    更新日期:1999-03-01 00:00:00

  • Protein expression analysis of chronic lymphocytic leukemia defines the effect of genetic aberrations and uncovers a correlation of CDK4, P27 and P53 with hierarchical risk.

    abstract:BACKGROUND:Chronic lymphocytic leukemia has a variable clinical course. Genomic aberrations identify prognostic subgroups, pointing towards distinct underlying biological mechanisms that are poorly understood. In particular it remains unclear whether the prognostic subgroups of chronic lymphocytic leukemia are characte...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2010.025734

    authors: Winkler D,Schneider C,Zucknick M,Bögelein D,Schulze K,Zenz T,Mohr J,Philippen A,Huber H,Bühler A,Habermann A,Benner A,Döhner H,Stilgenbauer S,Mertens D

    更新日期:2010-11-01 00:00:00

  • Treatment of acquired factor VIII inhibitor with recombinant activated factor VIIa: data from the Italian registry of acquired hemophilia.

    abstract::We report on the data collected in the Italian Registry of acquired haemophilia (AH) in 2001. Recombinant activated factor VII (rFVIIa) was selected as first-line therapy in 19 bleeding episodes because of their severity and as salvage in one case. Bleeding was controlled in 90% of the episodes, indicating the efficac...

    journal_title:Haematologica

    pub_type: 信件

    doi:

    authors: Baudo F,de Cataldo F,Gaidano G,Italian registry of acquired hemophilia.

    更新日期:2004-06-01 00:00:00

  • Aerobic glycolysis fuels platelet activation: small-molecule modulators of platelet metabolism as anti-thrombotic agents.

    abstract::Platelets are critical to arterial thrombosis, which underlies myocardial infarction and stroke. Activated platelets, regardless of the nature of their stimulus, initiate energy-intensive processes that sustain thrombus, while adapting to potential adversities of hypoxia and nutrient deprivation within the densely pac...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:10.3324/haematol.2018.205724

    authors: Kulkarni PP,Tiwari A,Singh N,Gautam D,Sonkar VK,Agarwal V,Dash D

    更新日期:2019-04-01 00:00:00

  • Efficient gene transfer into primitive hematopoietic progenitors using a bone marrow microenvironment cell line engineered to produce retroviral vectors.

    abstract:BACKGROUND AND OBJECTIVES:Effective gene transfer into human hematopoietic stem/progenitor cells is a compromise between achieving high transduction efficiency and maintaining the desired biological characteristics of the target cell. The aim of our work was to exploit the stromal microenvironment to increase gene tran...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: Dando JS,Ficara F,Deola S,Roncarolo MG,Bordignon C,Aiuti A

    更新日期:2004-04-01 00:00:00

  • Reticulocyte transferrin receptor (TfR) expression and contribution to soluble TfR levels.

    abstract:BACKGROUND AND OBJECTIVES:Transferrin receptor (TfR) expression in erythroid cells is regulated by a number of factors, including iron status and erythropoietin (Epo) stimulation. However, the impact of these factors on reticulocyte TfR expression in vivo has never been studied. A soluble form of TfR (sTfR) is present ...

    journal_title:Haematologica

    pub_type: 杂志文章

    doi:

    authors: R'zik S,Loo M,Beguin Y

    更新日期:2001-03-01 00:00:00