In vitro culture of peri-gastrulation embryos of a macropodid marsupial.

Abstract:

:Peri-gastrulation stage tammar wallaby embryos were cultured for up to 78 h in either Dulbecco's Modified Eagle's Medium or Medium 199, in air/6% CO(2) or 95% O(2)/5% CO(2), and with added fetal calf or wallaby serum. There was little difference between the two media or sera sources, but development was markedly superior for embryos cultured in 95% O(2)/5% CO(2). Many embryos survived even prolonged culture periods up to and over 70 h, and although development continued throughout the culture period, the embryos as a whole became increasingly abnormal. Embryos explanted at the primitive streak/ regressing node stages performed better in vitro than embryos explanted at earlier or later stages. The embryo that developed the furthest had a newly formed node at the initiation of culture and after 64 h in vitro it had developed forelimb ridges, fused, beating heart tubes and mesonephric ducts. Thus high oxygen appears to be the critical component of the culture system for optimal development of primitive streak stage tammar embryos. These results provide a basis for developing culture conditions for longer term development of marsupial embryos in vitro.

journal_name

J Anat

journal_title

Journal of anatomy

authors

Hickford D,Shaw G,Renfree MB

doi

10.1111/j.1469-7580.2007.00846.x

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

180-91

issue

2

eissn

0021-8782

issn

1469-7580

pii

JOA846

journal_volume

212

pub_type

杂志文章
  • A comparative multi-site and whole-body assessment of fascia in the horse and dog: a detailed histological investigation.

    abstract::Fascia in the veterinary sciences is drawing attention, such that physiotherapists and animal practitioners are now applying techniques based on the concept of fascia studies in humans. A comprehensive study of fascia is therefore needed in animals to understand the arrangement of the fascial layers in an unguligrade ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.13064

    authors: Ahmed W,Kulikowska M,Ahlmann T,Berg LC,Harrison AP,Elbrønd VS

    更新日期:2019-12-01 00:00:00

  • Radiographic features of bone in several strains of laboratory mice and of their tumours induced by bone-seeking radionuclides.

    abstract::The natural radiographic appearance of the various bones of the skeleton are described for several strains of laboratory mice. The Harwell substrains of CBA, A and 101 are generally similar and become osteoporotic on ageing. Harwell C57BL have similar, but more delicately chiseled, bones. Harwell C3H mice have bones w...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Loutit JF,Corp MJ,Ardran GM

    更新日期:1976-11-01 00:00:00

  • Patterns of morphological variation within myelin internodes of normal peripheral nerve: quantitative analysis by confocal microscopy.

    abstract::Knowledge of variations in the morphology of normal myelinated peripheral nerve fibres is fundamental to subsequent interpretation of neuropathology. It would be advantageous for structural analysis of normal variations to be based on entire myelin internodes, but acquisition of such data via the classic approach of n...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Reynolds RJ,Heath JW

    更新日期:1995-10-01 00:00:00

  • Distribution of acetylcholinesterase in the nerve endings of chicken m. retractor phalli cranialis.

    abstract::The distance of the neuromuscular gap was measured in a chicken smooth muscle, m. retractor phalli cranialis (MRPC). For the identification of active neuromuscular contacts, acetylcholinesterase (AChE) was stained according to Tago's modification of the Karnovsky and Roots' method. Thirty two out of 500 muscle cells w...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Ohsawa K,Nishida T,Kurohmaru M,Hayashi Y

    更新日期:1990-06-01 00:00:00

  • Rearrangement of extracellular matrix during cluster formation by human luteinising granulosa cells in culture.

    abstract::Human ovarian granulosa cells were cultured on a basement membrane preparation (Matrigel) to investigate the role of extracellular matrix components in granulosa cell cluster formation. Time-lapse videomicroscopy of these cultures revealed a rapid aggregation of cells which was initiated during the first 2-4 h of cult...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.2000.19620243.x

    authors: Richardson MC,Slack C,Stewart IJ

    更新日期:2000-02-01 00:00:00

  • Physiological in vitro sacroiliac joint motion: a study on three-dimensional posterior pelvic ring kinematics.

    abstract::The sacroiliac joint (SIJ) is a well-known source of low back and pelvic pain, of increasing interest for both conservative and surgical treatment. Alterations in the kinematics of the pelvis have been hypothesized as a major cause of SIJ-related pain. However, definitions of both the range and the extent of physiolog...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12924

    authors: Hammer N,Scholze M,Kibsgård T,Klima S,Schleifenbaum S,Seidel T,Werner M,Grunert R

    更新日期:2019-03-01 00:00:00

  • Elastic fibres in the vesicourethral junction and urethra of the guinea pig: quantification with computerised image analysis.

    abstract::Elastic fibres, which are intimately associated with collagen, a major component of the urethra, have been assumed to contribute to the resting urethral closure pressure. The Miller stain for elastin was used to demonstrate elastic fibres in cryostat sections of guinea pig bladder base, vesicourethral junction (VUJ) a...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.1999.19530447.x

    authors: Dass N,McMurray G,Brading AF

    更新日期:1999-10-01 00:00:00

  • The claustrum of the sheep and its connections to the visual cortex.

    abstract::The present study analyses the organization and selected neurochemical features of the claustrum and visual cortex of the sheep, based on the patterns of calcium-binding proteins expression. Connections of the claustrum with the visual cortex have been studied by tractography. Parvalbumin-immunoreactive (PV-ir) and Ca...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.13302

    authors: Pirone A,Graïc JM,Grisan E,Cozzi B

    更新日期:2021-01-01 00:00:00

  • The influence of age and particle number on absorption of polystyrene particles from the rat gut.

    abstract::The bioavailability of polystyrene particles (1 microns) labelled with FITC (3M Company, Düsseldorf) was tested in rats after enteral administration. Since macromolecules and particles are preferentially transported in the lymph, the number of particles was counted in the lymph of the thoracic duct over a 6 h period. ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Seifert J,Haraszti B,Sass W

    更新日期:1996-12-01 00:00:00

  • Digital development and morphogenesis.

    abstract::Signalling interactions between the polarizing region, which produces SHH, and the apical ectodermal ridge, which produces FGFs, are essential for outgrowth and patterning of vertebrate limbs. However, mechanisms that mediate translation of early positional information of cells into anatomy remain largely unknown. In ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.2003.00134.x

    authors: Sanz-Ezquerro JJ,Tickle C

    更新日期:2003-01-01 00:00:00

  • Structure of the pelvic and penile urethra--relationship with the ducts of the sex accessory glands of the Mongolian gerbil (Meriones unguiculatus).

    abstract::The urethra is the main place of entry for sexually transmitted pathogens. However, there is little literature on the morphology of the urogenital system, principally the urethra and ducts of the sex accessory glands. The Mongolian gerbil is an insectivorous, herbivorous and monogamous rodent with nocturnal habits; it...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.2003.00181.x

    authors: Pinheiro PF,Almeida CC,Segatelli TM,Martinez M,Padovani CR,Martinez FE

    更新日期:2003-05-01 00:00:00

  • Evidence of a hypermineralised calcified fibrocartilage on the human femoral neck and lesser trochanter.

    abstract::Femoral neck fractures are a major cause of morbidity and mortality in elderly humans. In addition to the age-related loss of cancellous bone, changes to the microstructure and morphology of the metaphyseal cortex may be a contributing factor in osteoporotic hip fractures. Recent investigations have identified a hyper...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.2001.19820153.x

    authors: Shea JE,Vajda EG,Bloebaum RD

    更新日期:2001-02-01 00:00:00

  • Vimentin, cytokeratin 8 and cytokeratin 18 are not specific markers for M-cells in human palatine tonsils.

    abstract::Standard immunohistochemical methods were used to detect the presence of vimentin, cytokeratin 8, cytokeratin 18, macrophages and Langerhans cells in the human tonsillar epithelium in formalin-fixed and frozen tissue specimens. Vimentin detection was restricted to infiltrating cells of the lymphoid series, dendritic a...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.2001.19960663.x

    authors: Koshi R,Mustafa Y,Perry ME

    更新日期:2001-12-01 00:00:00

  • Revisiting the infracardiac bursa using multimodal methods: topographic anatomy for surgery of the esophagogastric junction.

    abstract::In embryology, the infracardiac bursa (ICB) is a well-known derivative separated from the omental bursa. During surgeries around the esophagogastric junction (EGJ), surgeons often encounter a closed space considered to be equivalent to the ICB, but the macroscopic anatomy in adults is hardly known. This study aimed to...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12989

    authors: Nakamura T,Shinohara H,Okada T,Hisamori S,Tsunoda S,Obama K,Kurahashi Y,Takai A,Shimokawa T,Matsuda S,Makishima H,Takakuwa T,Yamada S,Sakai Y

    更新日期:2019-07-01 00:00:00

  • The effects of sex steroids on thyroid C cells and trabecular bone structure in the rat model of male osteoporosis.

    abstract::Androgen deficiency is one of the major factors leading to the development of osteoporosis in men. Since calcitonin (CT) is a potent antiresorptive agent, in the present study we investigated the effects of androgen deficiency and subsequent testosterone and estradiol treatment on CT-producing thyroid C cells, skeleta...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12013

    authors: Filipović B,Sošić-Jurjević B,Ajdžanović V,Pantelić J,Nestorović N,Milošević V,Sekulić M

    更新日期:2013-03-01 00:00:00

  • Observations on the development of the aortico-pulmonary spiral septum in the mouse.

    abstract::The differentiation of the bulbar region and arterial outflow tract of the developing mouse heart was investigated by analysing serial transverse sections through the heart region of mouse embryos isolated between the eleventh and fifteenth day of gestation. Over this period of time, we observed the configurational an...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Fananapazir K,Kaufman MH

    更新日期:1988-06-01 00:00:00

  • Morphological changes in the myenteric plexus of rat ileum after transection and end-to-end anastomosis.

    abstract::Surgical interruption of the gastrointestinal tract is widely used for investigating the structure of the enteric nervous system and in the treatment of certain pathological conditions of the gastrointestinal tract. The effects of transection and end-to-end anastomosis and myotomy on nerve cells of the myenteric plexu...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Karaosmanoğlu T,Müftüoğlu S,Dağdeviren A,Durgun B,Aygün B,Ors U

    更新日期:1996-04-01 00:00:00

  • Anatomy of the nerves and ganglia of the aortic plexus in males.

    abstract::It is well accepted that the aortic plexus is a network of pre- and post-ganglionic nerves overlying the abdominal aorta, which is primarily involved with the sympathetic innervation to the mesenteric, pelvic and urogenital organs. Because a comprehensive anatomical description of the aortic plexus and its connections...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12251

    authors: Beveridge TS,Johnson M,Power A,Power NE,Allman BL

    更新日期:2015-01-01 00:00:00

  • Contrast-enhanced micro-computed tomography of articular cartilage morphology with ioversol and iomeprol.

    abstract::Non-ionic, low-osmolar contrast agents (CAs) used for computed tomography, such as Optiray (ioversol) and Iomeron (iomeprol), are associated with the reduced risk of adverse reactions and toxicity in comparison with ionic CAs, such as Hexabrix. Hexabrix has previously been used for imaging articular cartilage but has ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.13271

    authors: Ter Voert CEM,Kour RYN,van Teeffelen BCJ,Ansari N,Stok KS

    更新日期:2020-12-01 00:00:00

  • Collateral arterial blood supply to the pregnant uterus in the sheep.

    abstract::The common internal iliac artery in the sheep gives rise to the arteries which are the primary source of blood to the uterus. We have used a vascular occluder placed on the common internal iliac artery of the pregnant sheep to induce and study the effects of chronic fetal hypoxaemia. We have noted that uterine blood f...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Mounts KO,Worrell MB,Boyle DW

    更新日期:1995-08-01 00:00:00

  • Ultrastructural and developmental features of the tessellated endoskeleton of elasmobranchs (sharks and rays).

    abstract::The endoskeleton of elasmobranchs (sharks and rays) is comprised largely of unmineralized cartilage, differing fundamentally from the bony skeletons of other vertebrates. Elasmobranch skeletons are further distinguished by a tessellated surface mineralization, a layer of minute, polygonal, mineralized tiles called tes...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12508

    authors: Seidel R,Lyons K,Blumer M,Zaslansky P,Fratzl P,Weaver JC,Dean MN

    更新日期:2016-11-01 00:00:00

  • Myofibroblasts in healing palatal wounds of the beagle dog.

    abstract::Excisional wounds were inflicted on the palatal mucoperiosteum of beagle pups at intervals from 3 to 31 days before the animals were killed, when the animals were perfused and the tissue examined by electron, microscopy. Fibroblasts with the morphological characteristics of myofibroblasts, including bundles of intrace...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Squier CA,Kremenak CR

    更新日期:1980-05-01 00:00:00

  • Quantitative description of the spatial arrangement of organelles in a polarised secretory epithelial cell: the salivary gland acinar cell.

    abstract::Previous quantitative descriptions of cellular ultrastructure have focused on spatial content (volume, surface area and number of organelles and membrane domains). It is possible to complement such descriptions by also quantifying spatial arrangements. Hitherto, applications of stereological methods for achieving this...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.1999.19420279.x

    authors: Mayhew TM

    更新日期:1999-02-01 00:00:00

  • The penile bone and anterior process of the rat in scanning electron microscopy.

    abstract::The penile bone and anterior process were made anorganic for scanning electron microscopy. The mature bone has a basal bulb and a long, cylindrical shaft bevelled at its tip to fit under a matching surface on the anterior process, giving rise to a bevelled 'suture'. A rough, resting, Sharpey-fibre bone surface covers ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Beresford WA,Burkart S

    更新日期:1977-12-01 00:00:00

  • Ontogeny of the cranial skeleton in a Darwin's finch (Geospiza fortis).

    abstract::Darwin's finches are a model system in ecological and evolutionary research, but surprisingly little is known about their skull morphology and development. Indeed, only the early beak development and external variation in adult beak shape has been studied. Understanding the development of the skull from embryo up to t...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/j.1469-7580.2011.01388.x

    authors: Genbrugge A,Heyde AS,Adriaens D,Boone M,Van Hoorebeke L,Dirckx J,Aerts P,Podos J,Herrel A

    更新日期:2011-08-01 00:00:00

  • Anatomy and function of cholinergic C bouton inputs to motor neurons.

    abstract::Motor control circuitry of the central nervous system must be flexible so that motor behaviours can be adapted to suit the varying demands of different states, developmental stages, and environments. Flexibility in motor control is largely provided by neuromodulatory systems which can adjust the output of motor circui...

    journal_title:Journal of anatomy

    pub_type: 杂志文章,评审

    doi:10.1111/joa.12063

    authors: Witts EC,Zagoraiou L,Miles GB

    更新日期:2014-01-01 00:00:00

  • The effects of pre- and postnatal exposure to the nonsteroidal antiandrogen flutamide on testis descent and morphology in the Albino Swiss rat.

    abstract::Exposure of male Albino Swiss rats to the nonsteroidal antiandrogen flutamide during the period from gestational day (d) 10 to birth resulted in feminisation of the external genitalia and the suppression of growth of the male reproductive tract. In adulthood, testes were found to be located in diverse positions. True ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1046/j.1469-7580.1997.19040577.x

    authors: Kassim NM,McDonald SW,Reid O,Bennett NK,Gilmore DP,Payne AP

    更新日期:1997-05-01 00:00:00

  • Bone histology in extant and fossil penguins (Aves: Sphenisciformes).

    abstract::Substantial changes in bone histology accompany the secondary adaptation to life in the water. This transition is well documented in several lineages of mammals and non-avian reptiles, but has received relatively little attention in birds. This study presents new observations on the long bone microstructure of penguin...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/joa.12367

    authors: Ksepka DT,Werning S,Sclafani M,Boles ZM

    更新日期:2015-11-01 00:00:00

  • Cell kinetics of growth cartilage in spondylo-metaphyseal chondrodysplasia (smc) mice.

    abstract::The gene for spondylo-metaphyseal chondrodysplasia (smc) in the mouse disrupts the formation of growth plate cartilage. No cartilage columns are found in the head of the tibia and secondary centres of ossification appear very late. The number of cells labelled with tritiated thymidine is sharply reduced at 16, 18 and ...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:

    authors: Thurston MN,Johnson DR,Kember NF

    更新日期:1985-05-01 00:00:00

  • Compliance in the neck structures of the guinea pig spermatozoon, as indicated by rapid freezing and electron microscopy.

    abstract::Electron microscopy has been used to investigate whether the transversely striated columns of the connecting piece in the neck region of guinea pig spermatozoa, undergo lengthening and shortening as a result of the forces generated during motility. Motile spermatozoa were subjected to near-instantaneous rapid freezing...

    journal_title:Journal of anatomy

    pub_type: 杂志文章

    doi:10.1111/j.1469-7580.2008.00919.x

    authors: Woolley DM,Carter DA,Tilly GN

    更新日期:2008-09-01 00:00:00