Abstract:
AIM OF THE STUDY:To evaluate in vitro the antiprotozoal and cytotoxic activities of 80% methanol extract from 45 medicinal plants collected in Sankuru (Democratic Republic of Congo) against Trypanosoma brucei brucei, Trypanosoma cruzi and the chloroquine-sensitive Ghanaian strain of Plasmodium falciparum, and MRC-5 cell lines respectively. MATERIAL AND METHODS:Different extracts were obtained by maceration of each plant part used with 80% methanol for 24h. The mixture was filtered and evaporated in vacuo to give corresponding dried extract. The activity against Trypanosoma brucei brucei and Trypanosoma cruzi were performed in 96 well tissue plates each containing 10 microl aqueous plant extract dilutions (100 to 0.01 microg/ml) with 10 microl of the parasite suspension cultured in Hirumi medium supplemented with 10% foetal calf serum, a solution of 2% penicillin/streptomycin (2% P/S) After 4 days incubation with Almar blueâ solution, fluorescence was measured at 500 nm emission and 530 nm excitation and results expressed as percentage reduction in parasite compared to control wells. The antiplasmodial activity of was assessed in vitro against the chloroquine-sensitive Ghanaian strain of Plasmodium falciparum cultured in RPMI-1640 medium by the lactate deshydrogenase assay in the presence of plant extracts (50 to 0.01 microg/ml). Cell-lines MRC-5 were cultured in MEM medium supplemented with 20mM l-glutamine, 16.5mM NaHCO(3), 5% foetal calf serum and 2% P/S solution. After 4h incubation, cell proliferation/viability was spectrophotomecally assessed at 540 nm after addition of MTT. In each assay, the IC50 value for each sample was derived by the drug concentration-response curves. RESULTS:The extracts from Alcornea cordifolia leaves, Momordica charantia whole plant, Omphalocarpum glomerata, root bark and Piptadia africanum stem bark showed good antiprotozoal activity against Trypanosoma brucei brucei with IC50 values from 0.7 to 7 microg/ml. Only Piptadenia africanum extract showed a pronounced antiprotozoal activity against Trypanosoma cruzi (IC50=4.0+/-06 microg/ml). The extracts from Alchornea cordifolia, Polyathia swaveleons stem bark, Sapium cornutum stem bark and Triclisia giletii stem bark exhibited a pronounced antiplasmodial activity against P. falciparum Ghanaian strain with IC50 values ranging from 0.5 to 3.0 microg/ml. Piptadenia africanum extract was the most cytotoxic sample (CC50=0.25 microg/ml) with poor selectivity against all selected protozoa (SI<10) while other active extracts did not show a significant cytotoxic effect against MCR-5 cell-lines with good selectivity according to the case. CONCLUSION:These active plant extracts are selected for extensive studies leading to the isolation of active constituents.
journal_name
J Ethnopharmacoljournal_title
Journal of ethnopharmacologyauthors
Mesia GK,Tona GL,Nanga TH,Cimanga RK,Apers S,Cos P,Maes L,Pieters L,Vlietinck AJdoi
10.1016/j.jep.2007.10.028subject
Has Abstractpub_date
2008-02-12 00:00:00pages
409-15issue
3eissn
0378-8741issn
1872-7573pii
S0378-8741(07)00555-7journal_volume
115pub_type
杂志文章abstract:ETHNOPHARMACOLOGICAL RELEVANCE:ANNAO tablets derive from Chinese classical prescriptions of Angong Niuhuang Pills with modified compositions, which have been singly or combined used for stoke associated neurological disorders. However the underlying mechanism is not yet well-defined, the present study investigated its ...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112212
更新日期:2020-01-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Celosia argentea L. (Amaranthaceae), popularly known as "crista de galo", is used in folk medicine due to its diuretic and hypotensive effects. However, there are no reports in the literature regarding its pharmacological effects on the cardiovascular system as well as no data proving the...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.09.027
更新日期:2019-01-30 00:00:00
abstract:ETHNOPHARMOCOLOGICAL RELEVANCE:Terminalia catappa Linn (Combretaceae) is a medicinal plant with anti-inflammatory, anti-diarrhoeal and antioxidant properties, frequently found in tropical regions. Considering its characteristics, it could be useful for the treatment of inflammatory bowel disease, which is associated wi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.07.056
更新日期:2016-11-04 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ficus religiosa (Moraceae) is reported to have numerous therapeutic utility in folk medicine. Among different biological activities on central nervous system, it has been reported to be used in ethnomedical treatment of epilepsy, which led us to further explore its anticonvulsant activity...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2009.02.042
更新日期:2009-06-22 00:00:00
abstract::This review explores medieval, ancient and modern sources for ethnopharmacological uses of Ficus (fig) species, specifically for employment against malignant disease and inflammation. The close connection between inflammatory/infectious and cancerous diseases is apparent both from the medieval/ancient merging of these...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.jep.2008.06.025
更新日期:2008-09-26 00:00:00
abstract::The purpose of this study was to characterize the putative anxiolytic-like activity of an ethanolic extract prepared from the leaves of Apocynum venetum (AV) using the elevated plus maze (EPM) in mice. Male C75BL/6 mice were either treated orally with the AV extract or the positive controls diazepam and buspirone, res...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.09.035
更新日期:2007-04-04 00:00:00
abstract::Salvia miltiorrhiza Bunge (Tanshen), a traditional Chinese herbal medicine, is popularly used to treat cardiovascular disorders. In the present study, effects of tanshinlactone A (C(16)H(12)O(4); M.W. 268), newly discovered from Salvia miltiorrhiza, on phytohemagglutinin (PHA)-stimulated cell proliferation were invest...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2007.05.026
更新日期:2007-09-05 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:The development of selective inhibitors of monoamine oxidase B (MAO-B) has been essential in treating Parkinson's disease. However, the apparent hepatotoxicity and drug-drug interactions of current inhibitors accentuate the need for the development of novel pharmacotherapies. Crossyne gut...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.112305
更新日期:2020-02-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:H. longipes S.F. Blake (Asteraceae) is a Mexican plant, whose roots are traditionally used as a condiment, as a mouth anesthetic, and as an antiparasitic. Affinin is the alkamide present in higher amounts in the roots of H. longipes. AIM OF THE STUDY:To date, there are no published studi...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.04.037
更新日期:2010-07-20 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Hertia cheirifolia L. is used traditionally to treat rheumatic pains and known as a medicinal plant having several pharmaceutical and biological activities. The present study evaluated in vivo the anti-inflammatory and gastroprotective effects of the methanolic extract from H. cheirifolia...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2018.02.010
更新日期:2018-05-10 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Plants from genus Lavandula have been used as anti-inflammatory drugs in Mediterranean traditional medicine. Nowadays, there is a growing interest for complementary medicine, including herbal remedies, to treat inflammatory bowel disease (IBD). AIM OF THE STUDY:To test the anti-inflammat...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.05.063
更新日期:2016-08-22 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Tongnao Decoction (TND) is a Chinese decoction approved and used in Jiangsu Province Hospital for the treatment of ischemic stroke. It shows conclusive efficiency in the improvement of neurologic impairment and activities of daily living of the patients. AIM OF THE STUDY:Recently, angiog...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112737
更新日期:2020-05-23 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Hericium is a genus of mushrooms (fungus) in the Hericiaceae family. Hericium erinaceus (HE) has been used for the treatment of digestive diseases for over 2000 years in China. HE possesses many beneficial functions such as anticancer, antiulcer, antiinflammation and antimicrobial effects...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.03.003
更新日期:2014-04-28 00:00:00
abstract:AIM OF THE STUDY:We sought to determine the efficacy as antioxidant and safety profile of the polyherbal formulation in geriatric patients of eastern India. MATERIALS AND METHODS:The study was double-blind, randomized including placebo controlled and was approved by the ethical committee of SSKM hospital. Geriatric pa...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jep.2010.12.044
更新日期:2011-03-24 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:[corrected] The seeds of Syzygium cumini, Skeels (Jamun) are extensively used in India for treatment of diabetes and other ailments. AIM OF THE STUDY:The aim of this work was to assess the role of Jamun seed extract (JSE) as a chemoprotective agent against in vivo oxidative stress and ge...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.12.014
更新日期:2011-03-24 00:00:00
abstract::The phytochemical, pharmacological and toxicological feature of plant Rhaphidophora pertusa (Roxb.) was done. Phytosteroids, flavonoids, tannins and glucosides were detected in the plant extracts. In cross-bred (Zebu X Holstein-Friesian or Jersey) dairy cows, subsequent to prostaglandin (PG) induced oestrus, to each g...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2006.03.036
更新日期:2006-10-11 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Jatropha curcas L. is a plant with high cultural significance for quilombola communities of Oriximiná (Pará State, Brazil). Although the plant is highly toxic, its seeds are used in these communities to treat tuberculosis and related diseases and symptoms. AIM OF THE STUDY:This study was...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2019.111970
更新日期:2019-09-15 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ocimum species are traditionally used for the treatment of anxiety, nerve pain, convulsions and a variety of neurodegenerative disorders. The present study was undertaken to evaluate the anti-amnesic effect of O. basilicum L., O. sanctum L. and O. gratissimum L. extracts using in-vitro an...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2016.10.026
更新日期:2016-12-04 00:00:00
abstract:AIM OF THE STUDY:To investigate the hypoglycemic effects of Vaccinium bracteatum Thunb. leaves (VBTL) extract in streptozotocin-induced diabetic mice. MATERIALS AND METHODS:After administration of VBTL extract for 4 weeks, the body weight, organ weight, blood glucose (BG), insulin and plasma lipid levels of streptozot...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2010.05.031
更新日期:2010-08-09 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Magnesium lithospermate B (MLB), an active polyphenol acid of Radix Salviae Miltiorrhizae (Danshen), showed a wide range of pharmacological activities in cardiovascular diseases. However, its role in protection against ischemia/reperfusion injury in H9c2 cardiomyocytes has not been elucid...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2013.11.036
更新日期:2014-01-01 00:00:00
abstract::The neuropharmacological activities of the aqueous extract of Diospyros mespiliformis stem bark were screened in mice. The extracts effect on pentobarbital-induced sleeping time, pentylenetetrazole induced seizure, spontaneous motor activity (SMA), exploratory behaviour, and rota-rod performance (motor coordination) w...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(02)00249-0
更新日期:2002-11-01 00:00:00
abstract::Viscum album L. has been used in the indigenous system of medicine for treatment of various diseases such as atherosclerosis and hypertension. In the literature, phenylpropan and flavonoid derivatives were suggested to play a role in the inhibition of cyclic adenosine monophosphate (cAMP)-phosphodiesterase (PDE) and a...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(00)00251-8
更新日期:2000-09-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Panax ginseng C.A. Meyer has been the most precious and renowned Chinese herb used in Asian countries for the treatment of various medical disorders. AIM OF THE STUDY:The aim of this work was to investigate the activation effect on murine peritoneal macrophages of oligosaccharide from th...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2012.09.004
更新日期:2012-12-18 00:00:00
abstract::Antitumor and immunomodulatory activities of an aqueous extract (GF100) of Acanthopanax senticosus were examined. In experimental lung metastasis of colon26-M3.1 carcinoma cells, intravenous (i.v.) administration of GF100 2 days before tumor inoculation significantly inhibited lung metastasis in a dose-dependent manne...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2004.03.052
更新日期:2004-08-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Qingwen Baidu Decoction (QBD), a famous traditional Chinese medicine prescription with heat-clearing and detoxifying efficacies, is widely used in the treatment of inflammatory diseases. However, due to lack of holistic quality evaluation research, the further study on the detailed molecu...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.113145
更新日期:2020-12-05 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:In African traditional medicine, Ageratum conyzoides has been used as purgative, febrifuge, anti-ulcer and wound dressing. To date there is no safety information about long term use of Ageratum conyzoides which contains pyrrolizidine alkaloids, a class of hepatotoxic and carcinogenic phyt...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2014.07.005
更新日期:2014-09-11 00:00:00
abstract::Seventy-seven crude extracts from leaves and stem barks of 15 Gabonese plants used in traditional medicine were evaluated for their cytotoxic, antileishmanial and antifungal activities. Most of the extracts exhibited cytotoxic activities toward human monocytes, and most particularly the hydromethanolic 50% (v/v) fract...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2005.06.011
更新日期:2005-11-14 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Ethnoveterinary medicine is often assumed to be a subset of human medicinal knowledge. Here we investigate the possibility that some ethnoveterinary medicine rather originates from observations of animal self-medication. We document and analyze the ethnoveterinary medicine used by Karen m...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2020.112823
更新日期:2020-09-15 00:00:00
abstract::Curculigo orchioides Gaerten belongs to the family Amaryllidaceae. The rhizomes of the plants are used for the treatment of decline in strength, jaundice and asthma. Its methanolic extract has been shown to enhance phagocytic activity of macrophages. Present studies have led to the isolation of two phenolic glycosides...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/s0378-8741(03)00160-0
更新日期:2003-12-01 00:00:00
abstract:ETHNOPHARMACOLOGICAL RELEVANCE:Hyptis martiusii (Lamiaceae), popularly known as "cidreira-do-mato" or "cidreira-brava", grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to charact...
journal_title:Journal of ethnopharmacology
pub_type: 杂志文章
doi:10.1016/j.jep.2011.07.005
更新日期:2011-09-01 00:00:00