A microwell array system for stem cell culture.

Abstract:

:Directed embryonic stem (ES) cell differentiation is a potentially powerful approach for generating a renewable source of cells for regenerative medicine. Typical in vitro ES cell differentiation protocols involve the formation of ES cell aggregate intermediates called embryoid bodies (EBs). Recently, we demonstrated the use of poly(ethylene glycol) (PEG) microwells as templates for directing the formation of these aggregates, offering control over parameters such as size, shape, and homogeneity. Despite these promising results, the previously developed technology was limited as it was difficult to reproducibly obtain cultures of homogeneous EBs with high efficiency and retrievability. In this study, we improve the platform by optimizing a number of features: material composition of the microwells, cell seeding procedures, and aggregate retrieval methods. Adopting these modifications, we demonstrate an improved degree of homogeneity of the resulting aggregate populations and establish a robust protocol for eliciting high EB formation efficiencies. The optimized microwell array system is a potentially versatile tool for ES cell differentiation studies and high-throughput stem cell experimentation.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Moeller HC,Mian MK,Shrivastava S,Chung BG,Khademhosseini A

doi

10.1016/j.biomaterials.2007.10.030

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

752-63

issue

6

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(07)00832-0

journal_volume

29

pub_type

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