Abstract:
:Double immunofluorescence studies using antibodies against NF-L and peripherin revealed three distinct subpopulations of neurons in rat dorsal root ganglia (DRG). In the adult rat, 46% of the DRG neurons were small and peripherin-positive (NF-L-negative), and 48% were large and NF-L-positive (peripherin-negative). About 6% were both peripherin- and NF-L-positive. All of the DRG neurons reacted with antibodies to NF-M and nonphosphorylation-dependent or phosphorylation-independent antibodies to NF-H. The neuropeptides were predominantly found in the peripherin-positive small cell population. Eighty-seven percent of the peripherin-positive small cell population contained substance P immunoreactivity, while 43% of this cell population contained CGRP. In contrast, only 18-24% of the NF-L-positive large-cell population contained neuropeptides, and these were primarily in a smaller sized subpopulation. Similar patterns of antigen representation were observed in neonatal (PN2) DRG cell populations. Tissue cultures of sensory ganglion cells from PN2 DRG, in serum-free medium, stably maintained exclusively peripherin-positive neurons, with about 5% of these containing coexistent NF-L immunoreactivity. Very high levels of neuropeptide gene expression were exhibited by these postnatal neurons in culture.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Goldstein ME,House SB,Gainer Hdoi
10.1002/jnr.490300111subject
Has Abstractpub_date
1991-09-01 00:00:00pages
92-104issue
1eissn
0360-4012issn
1097-4547journal_volume
30pub_type
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2004-11-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490160109
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journal_title:Journal of neuroscience research
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更新日期:1988-10-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290409
更新日期:1991-08-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20372
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490340602
更新日期:1993-04-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200306
更新日期:1988-07-01 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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