Abstract:
:Cryogenic temperature transmission electron microscopy (cryo-TEM) makes it possible to study the nanostructure of a wide range of fluid phases with a high degree of preservation. Most studies based on scanning electron microscopy or TEM employ specimen preparation techniques that give extraordinary results for tissues, but alter the native structure of complex fluid substances such as lung surfactant. In this paper, we evaluated direct-imaging cryo-TEM as a method to study the morphology of the aqueous form of lung surfactant. We compared the morphology of samples obtained from different species, and cryo-TEM data to data obtained by staining-and-drying. We demonstrate that cryo-TEM preserves and images much better sample morphology and fine details of the surfactant structures. We show that cryo-TEM, a method based on physical fixation, which avoids chemical changes and aggregate rearrangement, is a most useful tool to further our understanding of lung surfactant and its function.
journal_name
Clin Physiol Funct Imagingjournal_title
Clinical physiology and functional imagingauthors
Waisman D,Danino D,Weintraub Z,Schmidt J,Talmon Ydoi
10.1111/j.1475-097X.2007.00763.xsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
375-80issue
6eissn
1475-0961issn
1475-097Xpii
CPF763journal_volume
27pub_type
杂志文章abstract:OBJECTIVE:Levels of glucose and lactate were measured in the brain by means of microdialysis in order to evaluate the effects of ventilator-induced hypocapnia and hypercapnia on brain metabolism in healthy non-brain-traumatized animals. DESIGN AND SETTING:Prospective animal study in a university laboratory. SUBJECTS:...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2004.00557.x
更新日期:2004-07-01 00:00:00
abstract::New advances in computer processing and imaging have allowed the development of innovative techniques to assess lung function. A promising methodology is optoelectronic plethysmography (OEP). OEP evaluates ventilatory kinematics through the use of infrared imaging. Markers are placed, and images read on the chest, bac...
journal_title:Clinical physiology and functional imaging
pub_type: 历史文章,杂志文章,评审
doi:10.1111/j.1475-097X.2011.01028.x
更新日期:2011-09-01 00:00:00
abstract:AIM:Obstructive sleep apnoeas generate an intense afferent traffic leading to arousal and apnoea termination. Yet a decrease in the sensitivity of the afferents has been described in patients with obstructive sleep apnoea, and could be a determinant of disease severity. How mechanical changes within the respiratory sys...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2008.00830.x
更新日期:2009-01-01 00:00:00
abstract::The aim of the study was to collect new reference values for the clinical ramp exercise test on bicycle, because in our experience, the commonly used values were too low. A group of healthy men (n = 81, 20-80 years) was randomly selected from the local municipal register to achieve an even distribution in age. Data we...
journal_title:Clinical physiology and functional imaging
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1475-097x.2003.00491.x
更新日期:2003-05-01 00:00:00
abstract:INTRODUCTION:Notwithstanding recent progress on molecular mechanisms underlying heart failure with preserved ejection fraction (HFpEF), multiple pathophysiological aspects of this condition including the basis of anaemia and other haematological disorders remain unresolved. In this study, we sought to determine the rel...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12614
更新日期:2020-03-01 00:00:00
abstract::This study examined the effects of different periods of hypoxic training on glucose metabolism. Sedentary subjects underwent hypoxic training (FiO2 = 15.0%) for either 2 weeks (2-week group; n = 11) or 4 weeks (4-week group; n = 10). The 2-week group conducted training sessions on 6 days week(-1) for 2 weeks, whereas ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章,随机对照试验
doi:10.1111/cpf.12133
更新日期:2015-03-01 00:00:00
abstract:PURPOSE:The purpose of this study was to analyse gender differences in neuromuscular behaviour of the gastrocnemius and vastus lateralis during the take-off phase of a countermovement jump (CMJ), using direct measures (ground reaction forces, muscle activity and dynamic ultrasound). METHODS:Sixty-four young adults (ag...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12369
更新日期:2017-11-01 00:00:00
abstract:OBJECTIVE AND METHODS:We compared InBody720 segmental multifrequency bioimpedance analysis (SMF-BIA) with Lunar Prodigy Advance dual-energy X-ray absorptiometry (DXA) in assessment of body composition among 178 predominantly prepubertal children. Segmental agreement analysis of body compartments was carried out, and in...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12118
更新日期:2015-01-01 00:00:00
abstract::Phase-shift time curves following a bolus injection of gadolinium contrast agent were registered for grey-matter regions and large vessels in 14 subjects. Deconvolving a tissue phase-shift curve with a phase-based arterial input function resulted in a tissue residue function R(t). The peak value of R(t) provided a rel...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2010.00947.x
更新日期:2010-09-01 00:00:00
abstract::In the present study, hand-to-hand bioelectrical impedance analysis (BIA), skinfold (SKF) thickness and height-weight (body mass index, BMI)-based equations and dual-energy X-ray absorptiometry (DEXA), as a criterion method, were compared with each other in the assessment of body fat percentage (BF%) in 17-18-year-old...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2004.00534.x
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:The use of radioactive compounds for sentinel node biopsy is now a generally accepted part of the surgical treatment of breast cancer and melanoma, with the risk of radiation exposure to the operating team. The aim of this investigation was to study the levels of this exposure in relation to the permissible ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2005.00611.x
更新日期:2005-07-01 00:00:00
abstract::The relationships between electromyographic (EMG) activity and force as well as muscle blood flow and work have been well established. However, the association between muscle blood flow and EMG activity remains unsolved. Thus, to test the hypothesis that muscle EMG activity relates to muscle perfusion in different com...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2006.00656.x
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:In children, exercise-induced dyspnea is a common symptom that can be due to dysfunctional breathing. EVH test has bee used especially in elite athletes as bronchoprovocation test. Currently, there are only few studies on the EVH test. New research methods are required alongside the traditionally used tests ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12458
更新日期:2018-07-01 00:00:00
abstract::The purpose of this study was to determine whether exercise modulates the responses of middle cerebral artery blood velocity (MCA V(mean)) and cerebrovascular conductance to sympathetic stimulation (i.e. cold pressor test--CPT). To accomplish this, MCA V(mean) responses were assessed during CPT, static handgrip exerci...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1475-097X.2011.01055.x
更新日期:2012-01-01 00:00:00
abstract::We questioned whether electromyographic (EMG) signs of neuromuscular fatigue accompany the changes in respiratory variables measured at the ventilatory threshold (VTh) during exercise on a cycloergometer. This was based on the assumption that the activation of muscle afferents sensitive to accumulation of lactate and ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1046/j.1475-097x.2003.00497.x
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:To validate ironload T2* by automatic inline Maximum Likelihood Estimate (MLE) with k-space Rician noise correction, against the manual and automated truncation, as well as offset methods, in phantoms and in heart and liver in patients. METHODS:Twenty-five patients and an iron-oxide phantom were scanned at 1.5...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12303
更新日期:2017-05-01 00:00:00
abstract:UNLABELLED:The aim of the study was to validate the accuracy of the EXINI heart software (EXINI) package in assessing left ventricular end-diastolic/systolic volumes (EDV, ESV) and ejection fraction (LVEF) from gated (99m)Tc-MIBI single-photon emission tomography (SPECT). Cardiac magnetic resonance imaging (cMRI) was u...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2008.00840.x
更新日期:2009-04-01 00:00:00
abstract::Patients with nemaline myopathy, a muscle disorder primarily affecting the thin filaments, suffer from weakness which is poorly understood. As disturbed excitation-contraction coupling has been suggested as a possible mechanism, the present study was designed to investigate whether the contractile properties of the kn...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2007.00740.x
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Abdominal aortic aneurysm (AAA), a localized dilatation of the abdominal aorta, has a prevalence of about 1.5%-3% among 65- to 70-year-old males in Europe. AAA confers an increased risk of developing major cardiovascular events in addition to the risk of aneurysm rupture. The aim of this study was to evaluat...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12667
更新日期:2021-01-01 00:00:00
abstract::Literature does not agree with the usefulness of exercise blood pressure (BP) in predicting hypertension or target organ damage. In this prospective 10 years of follow-up, we evaluated if exaggerated BP responses to tests may improve the prediction of left ventricular mass index (LVMI). At baseline, BP was recorded by...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1046/j.1475-097x.2002.00412.x
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:Duplex ultrasound (DUS) has shown a >90% accuracy compared to angiography, concerning the degree of internal carotid artery (ICA) stenosis. However, uncertainty may occur in a severe stenosis, in which peak systolic velocity (PSV) may decrease owing to high flow resistance or high backward pressure. We inves...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2011.01118.x
更新日期:2012-05-01 00:00:00
abstract::Blood flow (BF) in exercising muscles is an important factor for exercise capacity. Recently, a non-invasive method to estimate capillary BF (Qcap ) was introduced. Using this method, the Fick principle is re-arranged by using relative differences in deoxygenated haemoglobin (ΔHHb) as a surrogate for arteriovenous O2 ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12434
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND AND AIMS:Our objective was to study relationships between the new biomarker of vascular health, carotid artery longitudinal wall motion (CALM) and metabolic syndrome (MetS). METHODS:Carotid ultrasound and assessment of MetS and its components were performed with 281 subjects aged 30-45 years. In the longitu...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/cpf.12687
更新日期:2020-12-19 00:00:00
abstract:BACKGROUND:Tissue dielectric constant (TDC) measurement may become an important tool in the clinical evaluation of chronic lower extremity swelling in women; however, several factors are known to influence TDC measurements, and comparative data on healthy lower extremities are few. METHODS:Thirty-four healthy women vo...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2012.01129.x
更新日期:2012-07-01 00:00:00
abstract::Volumetric capnography provides a breath-by-breath analysis of ventilation-perfusion imbalances and deadspace volumes. The technique has been best described in intubated and ventilated patients, but promising clinical applications also concern spontaneously breathing patients. The objective of the study was to verify ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2005.00620.x
更新日期:2005-09-01 00:00:00
abstract::Atherosclerosis is a growing health challenge globally, and despite our knowledge of the disease has increased over the last couple of decades, many unanswered questions remain. As molecular imaging can be used to visualize, characterize and measure biological processes at the molecular and cellular levels in living s...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章,评审
doi:10.1111/cpf.12017
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:Bronchial hyperresponsiveness (BHR) is common in bakery workers. The relation between bronchial responsiveness measured with a tidal breathing method and smoking, airway symptoms, IgE-sensitization, nasal indices of inflammation and flour dust exposure have been studied with bronchial responsiveness expresse...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1475-097X.2007.00755.x
更新日期:2007-09-01 00:00:00
abstract::The purpose was to investigate the acute effects of blood flow restriction (BFR) on arterial and venous hemodynamic parameters. Nine participants completed a 10-min time control (resting condition) and then a lower body BFR protocol. The protocol was five, 5-min bouts of restrictive cuff inflation with 3-min of deflat...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2012.01157.x
更新日期:2013-01-01 00:00:00
abstract:AIMS:To investigate the association between glucose abnormalities and left ventricular (LV) function assessed by tissue Doppler imaging (TDI) in patients with previous history of myocardial infarction and without known diabetes mellitus. PATIENTS AND METHODS:In a cross-sectional study, 123 patients, aged 31-80 years, ...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2007.00717.x
更新日期:2007-01-01 00:00:00
abstract::The primary aim of this study was to compare the maximal oxygen uptake as evaluated from a submaximal exercise test (EVO2peak) to direct measurements of VO2peak during a maximal exercise test as means of monitoring the aerobic endurance capacity in women with type 2 diabetes (T2D). Twenty-seven women with T2D particip...
journal_title:Clinical physiology and functional imaging
pub_type: 杂志文章
doi:10.1111/j.1475-097X.2008.00808.x
更新日期:2008-09-01 00:00:00