A novel role for Twist-1 in pulp homeostasis.

Abstract:

:The molecular mechanisms that maintain the equilibrium of odontoblast progenitor cells in dental pulp are unknown. Here we tested whether homeostasis in dental pulp is modulated by Twist-1, a nuclear protein that partners with Runx2 during osteoblast differentiation. Our analysis of Twist-1(+/-) mice revealed phenotypic changes that involved an earlier onset of dentin matrix formation, increased alkaline phosphatase activity, and pulp stones within the pulp. RT-PCR analyses revealed Twist-1 expression in several adult organs, including pulp. Decreased levels of Twist-1 led to higher levels of type I collagen and Dspp gene expression in perivascular cells associated with the pulp stones. In mice heterozygous for both Twist-1 and Runx2 inactivation, the phenotype of pulp stones appeared completely rescued. These findings suggest that Twist-1 plays a key role in restraining odontoblast differentiation, thus maintaining homeostasis in dental pulp. Furthermore, Twist-1 functions in dental pulp are dependent on its interaction with Runx2.

journal_name

J Dent Res

authors

Galler KM,Yasue A,Cavender AC,Bialek P,Karsenty G,D'Souza RN

doi

10.1177/154405910708601007

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

951-5

issue

10

eissn

0022-0345

issn

1544-0591

pii

86/10/951

journal_volume

86

pub_type

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