Abstract:
OBJECTIVES:Circulating levels of the proinflammatory mediator High Mobility Group Box Protein 1 (HMGB1) are increased in septic patients and may contribute to sepsis-induced organ dysfunction. Although HMGB1 has been shown to activate neutrophils from healthy volunteers, the responses of neutrophils from septic patients to HMGB1 have not been reported. In the present study we evaluated gene expression and activation of major intracellular signaling pathways in peripheral blood neutrophils obtained from patients with sepsis-induced acute lung injury after culture with HMGB1 or LPS. DESIGN:Ex-vivo study performed in neutrophils from patients with sepsis-induced acute lung injury. SETTING:Immunology and genetics laboratory at an academic medical center. PATIENTS AND PARTICIPANTS:Twenty-two adult patients with sepsis-induced acute lung injury. MEASUREMENTS AND RESULTS:Using gene arrays, distinct patterns of gene expression were found in neutrophils from septic patients after stimulation with HMGB1 or LPS. While more than three-quarters of the genes upregulated by HMGB1 in neutrophils from septic patients also demonstrated increased expression after culture with LPS, the majority of genes affected by LPS did not show altered expression in neutrophils stimulated with HMGB1. Culture of neutrophils with HMGB1 induced downregulation of its own expression, a finding not present after exposure to LPS. Although HMGB1 and LPS both increased nuclear translocation of NF-kappaB, the magnitude of this effect was greater in LPS stimulated neutrophils from patients with sepsis-induced acute lung injury. CONCLUSION:These findings demonstrate that the patterns of gene expression differ between neutrophils from septic patients stimulated with HMGB1 or LPS, and also that neutrophils from septic patients are not anergic but instead demonstrate intact activation of NF-kappaB after exposure to LPS or HMGB1.
journal_name
Intensive Care Medjournal_title
Intensive care medicineauthors
Silva E,Arcaroli J,He Q,Svetkauskaite D,Coldren C,Nick JA,Poch K,Park JS,Banerjee A,Abraham Edoi
10.1007/s00134-007-0748-2subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1829-39issue
10eissn
0342-4642issn
1432-1238journal_volume
33pub_type
杂志文章abstract:OBJECTIVE:Sepsis-evoked myocardial dysfunction is possibly due to decreased myofilament calcium sensitivity, and a calcium sensitizer may thus specifically improve contractility in sepsis by enhancing myofilament calcium sensitivity. We examined whether the calcium sensitizer levosimendan mitigates myocardial dysfuncti...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-003-1879-8
更新日期:2003-10-01 00:00:00
abstract::Placement of a Swan-Ganz catheter can cause many complications. This case report presents a new complication of Swan-Ganz catheter insertion--placement of the Swan-Ganz catheter tip in the coronary sinus. ...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF00254707
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:To determine the presence of tricuspid regurgitation (TR) in patients affected by acute lung injury (ALI) and the adult respiratory distress syndrome (ARDS) during mechanical ventilation with positive end-expiratory pressure (PEEP). DESIGN:A prospective clinical study. SETTING:10-bed general intensive care ...
journal_title:Intensive care medicine
pub_type: 临床试验,杂志文章
doi:10.1007/s001340050419
更新日期:1997-08-01 00:00:00
abstract:PURPOSE:To provide evidence-based guidelines for early enteral nutrition (EEN) during critical illness. METHODS:We aimed to compare EEN vs. early parenteral nutrition (PN) and vs. delayed EN. We defined "early" EN as EN started within 48 h independent of type or amount. We listed, a priori, conditions in which EN is o...
journal_title:Intensive care medicine
pub_type: 杂志文章,meta分析,实务指引,评审
doi:10.1007/s00134-016-4665-0
更新日期:2017-03-01 00:00:00
abstract:: ...
journal_title:Intensive care medicine
pub_type: 杂志文章,评审
doi:10.1007/s00134-018-5107-y
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:Antithrombin exerts direct effects on neutrophils by inhibiting chemokine-induced migration. This study examined the potency of different pharmaceutical antithrombin preparations in inhibiting neutrophil chemotaxis toward interleukin 8. METHODS:Cell migration was tested by the leading front assay in modified...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-002-1432-1
更新日期:2002-10-01 00:00:00
abstract:UNLABELLED:Admission of cancer patients with serious medical complications to the ICU remains controversial primarily because of the high short-term mortality rates in these patients. However, the cancer patient population is heterogeneous regarding age, underlying conditions, and curability of their disease, suggestin...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s001340051350
更新日期:2000-12-01 00:00:00
abstract:PURPOSE:During neurally adjusted ventilatory assist (NAVA) the ventilator is driven by the patients electrical activation of the diaphragm (EAdi), detected by a special esophageal catheter. A reliable positioning of the EAdi-catheter is mandatory to trace a representative EAdi signal. We aimed to determine whether a fo...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-009-1587-0
更新日期:2009-10-01 00:00:00
abstract:PURPOSE:Inflammatory markers may have a role in predicting severity of illness of intensive care unit (ICU) patients. The aim of this study is to determine whether low eosinophil count can predict 28-day mortality in medical ICU. METHODS:A prospective study over a 4-month period. To evaluate the prognosis information ...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-011-2170-z
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:Meconium aspiration syndrome (MAS) is a life-threatening neonatal lung injury, whose pathophysiology has been mainly studied in animal models. In such models, pancreatic secretory phospholipase A2 (sPLA2-IB) and proinflammatory cytokines present in meconium challenge the lungs, catabolising surfactant and harmi...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-011-2243-z
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To document the costs and outcomes of the various forms of the septic syndromes [systemic inflammatory response syndrome (SIRS), sepsis, severe sepsis, septic shock), particularly those associated with infection acquired in an intensive care unit (ICU). DESIGN:Prospective data collection for all septic patie...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-003-1877-x
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVE:To investigate plasma high-mobility group box 1 protein (HMGB1) concentration and its relationship with organ dysfunction and outcome in septic shock patients. DESIGN AND SETTING:Prospective, noninterventional study. Medical adult intensive care unit at a university hospital in France. PATIENTS:42 criticall...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-007-0691-2
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence, risk factors, and outcomes of critical illness neuromuscular abnormalities (CINMA). DESIGN:Systematic review. DATA SOURCES AND STUDY SELECTION:MEDLINE, EMBASE, CINAHL, and the Cochrane Library were searched for reports on adult ICU patients who were evaluated for CINMA clinicall...
journal_title:Intensive care medicine
pub_type: 杂志文章,评审
doi:10.1007/s00134-007-0772-2
更新日期:2007-11-01 00:00:00
abstract::Since the introduction of synchronized intermittent mandatory ventilation (SIMV) several advantages have been attributed to this ventilatory mode, one of them being a more homogeneous distribution of ventilation and perfusion than during controlled mechanical ventilation (CMV). Up to now no data are available to confi...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF01704716
更新日期:1991-01-01 00:00:00
abstract:INTRODUCTION:Extracorporeal carbon dioxide removal (ECCO2R) has been proposed for hypercapnic respiratory failure in chronic obstructive pulmonary disease (COPD) exacerbations, to avoid intubation or reduce length of invasive ventilation. Balance of risks, efficacy, and benefits of ECCO2R in patients with COPD is uncle...
journal_title:Intensive care medicine
pub_type: 杂志文章,评审
doi:10.1007/s00134-015-3921-z
更新日期:2015-10-01 00:00:00
abstract:PURPOSE:Reporting neurodevelopmental outcome of 8-year-old children treated with neonatal extracorporeal membrane oxygenation (ECMO). METHODS:In a follow-up study in 135 8-year-old children who received neonatal ECMO between 1996 and 2001 we assessed intelligence (Revised Amsterdam Intelligence Test), concentration (B...
journal_title:Intensive care medicine
pub_type: 杂志文章,多中心研究
doi:10.1007/s00134-013-2973-1
更新日期:2013-09-01 00:00:00
abstract::Results of the relationships between oxygen supply, demand, and uptake can be used to interpret cardiac output values, identify types of acute circulatory failure, guide attempts to improve cellular function, and thus prevent the development of multiple organ failure and death. Five steps in the interpretation of card...
journal_title:Intensive care medicine
pub_type: 杂志文章,评审
doi:10.1007/BF01785244
更新日期:1990-01-01 00:00:00
abstract::Membranous laryngotracheobronchitis is a very serious infection which affects the larynx, trachea and bronchi, requiring aggressive therapeutic measures. It has been recently rediscovered as a cause of disease in children. However, it is a very unusual complication of measles. Two infants with measles and membranous l...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF00273547
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:It remains uncertain why immunocompetent patients with bacterial community-acquired pneumonia (CAP) die, in spite of adequate antibiotics. METHODS:This is a secondary analysis of the CAPUCI database which was a prospective observational multicentre study. Two hundred and twelve immunocompetent patients admi...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-008-1363-6
更新日期:2009-03-01 00:00:00
abstract:PURPOSE:Little evidence of increased thrombotic risk is available in COVID-19 patients. Our purpose was to assess thrombotic risk in severe forms of SARS-CoV-2 infection. METHODS:All patients referred to 4 intensive care units (ICUs) from two centers of a French tertiary hospital for acute respiratory distress syndrom...
journal_title:Intensive care medicine
pub_type: 杂志文章,多中心研究
doi:10.1007/s00134-020-06062-x
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:To evaluate the development of demographics and outcome of very old (>80 years) critically ill patients admitted to intensive care units. SETTING:All consecutive patients admitted to 41 Austrian intensive care units (ICUs) over an 11-year period. METHODS:We performed a retrospective cohort study of prospectiv...
journal_title:Intensive care medicine
pub_type: 杂志文章,多中心研究
doi:10.1007/s00134-012-2474-7
更新日期:2012-04-01 00:00:00
abstract::The purpose of this study was noninvasive assessment of respiratory compliance and resistance in mechanically ventilated patients with acute respiratory failure (ARF). To this end, flow, change in lung volume, and airway pressure were measured at the proximal tip of the endotracheal tubes in twenty nine critically ill...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF00263528
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:To compare haemodynamic and gas exchange effects after either atracurium or pancuronium given to ventilated neonates with respiratory failure. DESIGN:In this prospective study neonates were alternately assigned to receive either atracurium or pancuronium, intravenously. SETTING:Paediatric and neonatal ICU i...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF01724880
更新日期:1993-01-01 00:00:00
abstract::Total extracorporeal lung assist (ECLA) requires a bypass flow approaching cardiac output. Recirculation of venous blood through the oxygenator is minimized with a veno-right ventricular cannulation technique which separates venous drainage from returned oxygenated blood. A case of posttraumatic ARDS was treated with ...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF01691426
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:The efficacy of interferon gamma therapy in reducing infection and improving outcome from infection in patients sustaining major injury was examined. DESIGN:Randomized double-blind placebo control trial SETTING:Nine level one university affiliated trauma centers in the United States. PATIENTS AND PARTICIPA...
journal_title:Intensive care medicine
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1007/BF01743725
更新日期:1996-10-01 00:00:00
abstract:OBJECTIVE:To measure the effects of rapid permissive hypercapnia on hemodynamics and gas exchange in patients with acute respiratory distress syndrome (ARDS). DESIGN:Prospective study. SETTING:18-bed, medical intensive care unit, university hospital. PATIENTS:11 mechanically ventilated ARDS patients. INTERVENTION:P...
journal_title:Intensive care medicine
pub_type: 临床试验,杂志文章
doi:10.1007/BF01712235
更新日期:1996-03-01 00:00:00
abstract::Arrhythmias are extremely common early after AMI. An arrhythmia is defined by exclusion, either because the sequence of myocardial depolarisation is other than normal or because certain arbitrary limits are exceeded. It follows that the term "arrhythmia" encompasses a complex heterogenous group. Although arrhythmias a...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/BF01683131
更新日期:1978-01-01 00:00:00
abstract:OBJECTIVE:To evaluate whether soluble triggering receptor expressed on myeloid cells 1 (sTREM-1) in CSF can serve as a biomarker for the presence of bacterial meningitis and outcome in patients with this disease. DESIGN:Retrospective study of diagnostic accuracy. SETTING AND PATIENTS:CSF was collected from 92 adults ...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-006-0240-4
更新日期:2006-08-01 00:00:00
abstract:PURPOSE:We evaluated whether oscillatory compliance (C(X5)) measured by forced oscillation technique (FOT) at 5 Hz may be useful for positive end-expiratory pressure (PEEP) optimisation. METHODS:We studied seven pigs in which lung injury was induced by broncho-alveolar lavage. The animals were ventilated in volume con...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-011-2211-7
更新日期:2011-06-01 00:00:00
abstract:PURPOSE:Extracorporeal life support (ECLS) is used for patients with refractory heart failure with or without respiratory failure. This temporary support is provided by blood pumps which are connected to large vessels. Bleeding episodes are a typical complication in patients with ECLS. Recently, several studies illustr...
journal_title:Intensive care medicine
pub_type: 杂志文章
doi:10.1007/s00134-011-2370-6
更新日期:2012-01-01 00:00:00