Role of ERK1/2 signaling pathways in 4-aminopyridine-induced rat pulmonary vasoconstriction.

Abstract:

:The aim of the present study was to investigate the contribution of extracellular signal regulated kinase-1/2 (ERK1/2) to pulmonary artery contraction in response to 4-aminopyridione (4-AP), an inhibitor of a voltage-gated K(+) channels that regulate pulmonary vascular tone. Pulmonary artery rings 1-1.5 mm in diameter from male adult Wistar rat were isolated and cut into 3-mm in length. ERK1/2 up-stream kinase (MEK) inhibitors 2'-amino-3'-methoxyflavone (PD98059) and 1,4-diamino-2,3-dicyano-1,4-bis (2-aminophenylthio)-butadiene (U0126), which block the activation of ERK1/2, were used to test the role of ERK1/2 in 4-AP induced pulmonary arterial vasoconstriction and the influences of 4-AP on expressions of phosphorylated ERK1/2 (p-ERK1/2) in cultured rat pulmonary arterial smooth muscle cells (PASMCs) and whole tissues. Our results show that 4-AP elicited concentration-dependent increases in tension of rat pulmonary artery rings, effects that were reduced by pretreatment of the rings with ERK inhibitors, PD98059 (20 microM) and U0126 (10 microM). Moreover, 4-AP increased the expressions of p-ERK1/2 in cultured PASMCs s and whole tissues, which were prevented by pretreatment of the cells or tissues with U0126. These results indicate that ERK1/2 signaling pathway contributes to pulmonary vasoconstriction induced by 4-AP.

journal_name

Eur J Pharmacol

authors

Han W,Tang X,Wu H,Liu Y,Zhu D

doi

10.1016/j.ejphar.2007.04.042

subject

Has Abstract

pub_date

2007-08-13 00:00:00

pages

138-44

issue

1-2

eissn

0014-2999

issn

1879-0712

pii

S0014-2999(07)00520-1

journal_volume

569

pub_type

杂志文章
  • Two populations of neurotensin binding sites in murine brain: discrimination by the antihistamine levocabastine reveals markedly different radioautographic distribution.

    abstract::Monoiodo-[125I-Tyr3]neurotensin (NT) bound to a high affinity, low capacity binding component and a lower affinity, high capacity component in rat brain synaptic membranes. The antihistamine H1 agent levocabastine, which bears no structural relationship to NT, selectively and totally inhibited NT binding to its low af...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(87)90285-8

    authors: Kitabgi P,Rostène W,Dussaillant M,Schotte A,Laduron PM,Vincent JP

    更新日期:1987-08-21 00:00:00

  • Effect of P-glycoprotein modulators on the human extraneuronal monoamine transporter.

    abstract::The aim of this work was to investigate the effect of P-glycoprotein modulators on human extraneuronal monoamine transporter (EMT)-mediated transport. The experiments were performed using a cell line from human embryonic kidney (HEK293 cells) stably transfected with pcDNA3hEMT (293(hEMT)), or with pcDNA3 alone (293(co...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(01)01055-x

    authors: Martel F,Keating E,Azevedo I

    更新日期:2001-06-22 00:00:00

  • Coptisine ameliorates DSS-induced ulcerative colitis via improving intestinal barrier dysfunction and suppressing inflammatory response.

    abstract::Ulcerative colitis (UC), as an autoimmune disease, has been troubling human health for many years. Up to now, the available treatments remain unsatisfactory. Rhizoma Coptidis has been widely applied to treat gastrointestinal diseases in China for a long time, and coptisine (COP) is identified as one of its major activ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2021.173912

    authors: Wang Y,Liu J,Huang Z,Li Y,Liang Y,Luo C,Ni C,Xie J,Su Z,Chen J,Li C

    更新日期:2021-01-25 00:00:00

  • Carbamazepine up-regulates the binding of [3H]PK 11195 to platelets of epileptic patients.

    abstract::The maximal number of binding sites (Bmax) and the equilibrium dissociation constant (KD) values for [3H]PK 11195 binding were determined in the platelet membranes of epileptic patients before and after 4 weeks of carbamazepine (CBZ) treatment, as well as in healthy controls. There was a decrease of 17.1% in the Bmax ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(87)90567-x

    authors: Weizman A,Tanne Z,Karp L,Martfeld Y,Tyano S,Gavish M

    更新日期:1987-09-23 00:00:00

  • Mechanistic characterization of S 38093, a novel inverse agonist at histamine H3 receptors.

    abstract::Histaminergic H3 inverse agonists, by stimulating central histamine release, represent attractive drug candidates to treat cognitive disorders. The present studies aimed to describe the mechanistic profile of S 38093 a novel H3 receptors inverse agonist. S 38093 displays a moderate affinity for rat, mouse and human H3...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2017.03.013

    authors: Sors A,Panayi F,Bert L,Favale D,Nosjean O,Audinot V,Arrang JM,Buisson B,Steidl E,Delbos JM,Huhtala T,Kontkanen O,Chollet AM,Casara P,Lestage P

    更新日期:2017-05-15 00:00:00

  • Characterization of 5-HT1A receptor-mediated [35S]GTPgammaS binding in rat hippocampal membranes.

    abstract::Stimulation of [35S]GTPgammaS binding by serotonin (5-hydroxytryptamine, 5-HT) receptor ligands was characterized in rat hippocampal membranes. The optimized assay contained 30-50 microg protein, 300 microM GDP and 0.1 nM [35S]GTPgammaS, incubated at 37 degrees C for 20 min. At 10 microM, the 5-HT1A receptor agonist R...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(97)01547-1

    authors: Alper RH,Nelson DL

    更新日期:1998-02-19 00:00:00

  • Dopamine receptor plasticity following MPTP-induced nigrostriatal lesions in the mouse.

    abstract::MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) destroys dopamine-containing nigrostriatal neurons and increases the apparent Bmax of both D1 and D2 binding sites in the striatum. However, the changes of Bmax occur at different intervals after the lesion. Up-regulation of D2 sites becomes evident about 3 weeks aft...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(90)90324-y

    authors: Weihmuller FB,Bruno JP,Neff NH,Hadjiconstantinou M

    更新日期:1990-05-16 00:00:00

  • Block of the transient inward current by R56865 in guinea-pig ventricular myocytes.

    abstract::Single myocytes isolated from the guinea-pig ventricle were used under voltage-clamp conditions to study the activity of a newly developed drug, R56865, a benzothiazolamine derivative, against digitalis toxicity. Multicellular Purkinje preparations were used in some experiments. The drug blocked the transient inward c...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(91)90407-h

    authors: Leyssens A,Carmeliet E

    更新日期:1991-04-10 00:00:00

  • Electrophysiological and neurochemical investigations on the action of carbamazepine on the rat hippocampus.

    abstract::Carbamazepine moderately depressed the input fiber volley resulting in attenuation of the dendritic epsp and the population spike in CA1 of rat hippocampal slices with a threshold concentration of 20 microM. The depressant effect on the population spike was not antagonized by the adenosine receptor blocker caffeine. P...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(85)90030-5

    authors: Olpe HR,Baudry M,Jones RS

    更新日期:1985-03-26 00:00:00

  • Molecular cloning, developmental expression and pharmacological characterization of the CCKB/gastrin receptor in the calf pancreas.

    abstract::We have cloned the calf predominant pancreatic cholecystokinin B (CCKB)/gastrin receptor cDNA. It encodes a 454 amino acid protein with 90% identity with the CCKB/gastrin receptor cloned in other species and tissues. However, the calf pancreatic CCKB/gastrin receptor contains a pentapeptide cassette within the third i...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(95)00737-7

    authors: Dufresne M,Escrieut C,Clerc P,Le Huerou-Luron I,Prats H,Bertrand V,Le Meuth V,Guilloteau P,Vaysse N,Fourmy D

    更新日期:1996-02-15 00:00:00

  • Kainic acid stimulates GABA release from a subpopulation of cerebellar astrocytes.

    abstract::The effect of acute exposure to micromolar concentrations of kainic acid was studied in rat cerebellar astroglial cultures consisting of two antigenically and functionally distinct subpopulations of astrocytes. Kainate (20-100 microM) released [3H]GABA preaccumulated by A2B5-positive stellate astrocytes in a concentra...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(86)90624-2

    authors: Gallo V,Suergiu R,Levi G

    更新日期:1986-12-16 00:00:00

  • Involvement of the strychnine-sensitive glycine receptor in the anxiolytic effects of GlyT1 inhibitors on maternal separation-induced ultrasonic vocalization in rat pups.

    abstract::Several studies have shown that glycine transporter 1 (GlyT1) inhibitors have anxiolytic actions. There are two types of glycine receptor: the strychnine-sensitive glycine receptor (GlyA) and the strychnine-insensitive glycine receptor (GlyB); however, which receptor is the main contributor to the anxiolytic actions o...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2014.11.024

    authors: Komatsu H,Furuya Y,Sawada K,Asada T

    更新日期:2015-01-05 00:00:00

  • Mangiferin alleviates lipopolysaccharide and D-galactosamine-induced acute liver injury by activating the Nrf2 pathway and inhibiting NLRP3 inflammasome activation.

    abstract::Mangiferin, a glucosylxanthone from Mangifera indica, has been reported to have anti-inflammatory effects. However, the protective effects and mechanisms of mangiferin on liver injury remain unclear. This study aimed to determine the protective effects and mechanisms of mangiferin on lipopolysaccharide (LPS) and D-gal...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2015.12.006

    authors: Pan CW,Pan ZZ,Hu JJ,Chen WL,Zhou GY,Lin W,Jin LX,Xu CL

    更新日期:2016-01-05 00:00:00

  • Big endothelin-1 and big endothelin-3 are constrictor agents in the microvasculature: evidence for the local phosphoramidon-sensitive conversion of big endothelin-1.

    abstract::The effect of local administration of big endothelin-1 (1-38, big ET-1) has been investigated on blood flow in the skin of anaesthetised rats, measured by 133Xe clearance. The local effects of big ET-1 and big endothelin-3 (1-41, big ET-3) have also been determined on microvessel diameter by intravital microscopy of t...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(93)90056-n

    authors: Lawrence E,Brain SD

    更新日期:1993-03-23 00:00:00

  • Characterisation using microphysiometry of CRF receptor pharmacology.

    abstract::We have assessed the utility of the Cytosensor microphysiometer for studying the pharmacology of recombinant CRF receptors. Chinese hamster ovary cells stably expressing the human CRF1 or CRF2 receptor were perfused in the Cytosensor with bicarbonate-free Hams F12 (pH 7.4) containing 0.2% bovine serum albumin. The ran...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(99)00506-3

    authors: Smart D,Coppell A,Rossant C,Hall M,McKnight AT

    更新日期:1999-08-27 00:00:00

  • The inflammatory reaction in an experimental model of open wounds in the rat. The effect of arachidonic acid metabolites.

    abstract::The study concerned the effect of arachidonic acid metabolites on the inflammatory reaction in granulation tissue of open wounds in rats. Metabolites or inhibitors were applied in a wound chamber attached to circular, open, full-thickness skin wounds 5 days post-wounding. The adjacent wound served as control. Blood fl...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(84)90454-0

    authors: Lundberg C,Gerdin B

    更新日期:1984-01-27 00:00:00

  • Differential effects of tyrosine kinase inhibitors on contraction and relaxation of the aortas of normotensive and hypertensive rats.

    abstract::The contribution of tyrosine kinase activity to vasoreactivity in normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats was investigated on isolated aortic preparations by the use of two tyrosine kinase inhibitors: methyl-2,5-dihydroxycinnamate (30 microM) and genistein (30 microM). The pretreatmen...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(99)00304-0

    authors: Zerrouk A,Auguet M,Dabiré H,Brisac AM,Safar M,Chabrier PE

    更新日期:1999-06-11 00:00:00

  • Mitomycin C induces fibroblasts apoptosis and reduces epidural fibrosis by regulating miR-200b and its targeting of RhoE.

    abstract::Mitomycin C (MMC) is known to reduce epidural fibrosis, but the underlying mechanisms have not yet been elucidated. Aberrant miR-200b expressions have been reported in multiple types of fibrotic tissues from many diseases. The aim of this study was to clarify the mechanism by which MMC induces fibroblasts apoptosis an...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2015.08.002

    authors: Sun Y,Ge Y,Fu Y,Yan L,Cai J,Shi K,Cao X,Lu C

    更新日期:2015-10-15 00:00:00

  • Putrescine modulation of acute activation of the beta-adrenergic system in the left atrium of rat.

    abstract::Endogenous polyamines mediate acute metabolic effects and cardiac hypertrophy associated to beta-adrenoceptor stimulation. The aim of this study is to characterize the role of polyamines on beta-adrenoceptor system mediated responses. To this end, the functional interaction of polyamine modifying drugs on isoprotereno...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2008.07.069

    authors: Bordallo C,Cantabrana B,Velasco L,Secades L,Meana C,Méndez M,Bordallo J,Sánchez M

    更新日期:2008-11-19 00:00:00

  • Increased resting Ca2+ maintains the myogenic tone and activates K+ channels in arteries from young spontaneously hypertensive rats.

    abstract::We examined whether the Ca2+ channel function in the resting state alters the resting tone in femoral and carotid arteries from spontaneously hypertensive rats (SHR) at early hypertensive stages (6 and 4 weeks of age), and data were compared with findings in age-matched normotensive Wistar-Kyoto rats (WKY). Strips of ...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0922-4106(93)90198-i

    authors: Asano M,Matsuda T,Hayakawa M,Ito KM,Ito K

    更新日期:1993-11-15 00:00:00

  • Intranasal cocaine functions as reinforcer on a progressive ratio schedule in humans.

    abstract::Cocaine dependence continues to be a worldwide public health concern. Although the majority of individuals reporting cocaine use do so via the intranasal route, relatively few laboratory experiments have examined the reinforcing effects of cocaine administered intranasally. The purpose of this experiment was to measur...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2010.06.055

    authors: Stoops WW,Lile JA,Glaser PE,Hays LR,Rush CR

    更新日期:2010-10-10 00:00:00

  • Antihyperalgesic effects of spinal cannabinoids.

    abstract::Cannabinoids have been widely reported to produce antinociception in models such as tail flick and hot plate. However, their role in modulating thermal hyperalgesia is unknown. The potency of some drugs, such as the opioids, increases during hyperalgesia. Thus, we evaluated whether there is a change in the effectivene...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(97)01621-x

    authors: Richardson JD,Aanonsen L,Hargreaves KM

    更新日期:1998-03-19 00:00:00

  • Pharmacological inhibition of GPR4 remediates intestinal inflammation in a mouse colitis model.

    abstract::Inflammatory bowel disease (IBD) is characterized by chronic, recurring inflammation of the digestive tract. Current therapeutic approaches are limited and include biologics and steroids such as anti-TNFα monoclonal antibodies and corticosteroids, respectively. Significant adverse drug effects can occur for chronic us...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2019.03.038

    authors: Sanderlin EJ,Marie M,Velcicky J,Loetscher P,Yang LV

    更新日期:2019-06-05 00:00:00

  • In vivo evidence that EMD 57033 restores myocardial responsiveness to intracoronary Ca(2+) in stunned myocardium.

    abstract::Despite ample in vitro evidence that myofilament Ca(2+)-responsiveness of stunned myocardium is decreased, in vivo data are inconclusive. Conversely, while Ca(2+)-sensitizing agents increase myofilament Ca(2+)-responsiveness in vitro, it has been questioned whether this also occurs in vivo. We therefore tested in open...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(00)00513-6

    authors: de Zeeuw S,Trines SA,Krams R,Duncker DJ,Verdouw PD

    更新日期:2000-09-01 00:00:00

  • SR 48968 specifically depresses neurokinin A- vs. substance P-induced hyperalgesia in a nociceptive withdrawal reflex.

    abstract::To determine the role of neurokinin A and tachykinin NK2 receptors in processing of nociceptive information at the spinal level, the selective NK2 receptor antagonist, SR 48968 (S)-N-methyl-N [4-(4-acetylamino-4-[phenyl piperidino)-2-(3,4-dichlorophenyl)-butyl] benzamide, was tested for its effects on the hyperalgesia...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(96)00262-2

    authors: Yashpal K,Hui-Chan CW,Henry JL

    更新日期:1996-07-11 00:00:00

  • Psychological co-morbidities in COPD: Targeting systemic inflammation, a benefit for both?

    abstract::COPD is a chronic lung disease characterized by persistent respiratory symptoms and airflow limitation due to airway and/or alveolar abnormalities. Furthermore, COPD is often characterized by extrapulmonary manifestations and comorbidities worsening COPD progression and quality of life. A neglected comorbidity in COPD...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejphar.2018.10.001

    authors: Pelgrim CE,Peterson JD,Gosker HR,Schols AMWJ,van Helvoort A,Garssen J,Folkerts G,Kraneveld AD

    更新日期:2019-01-05 00:00:00

  • Modulatory effect of imetit, a histamine H3 receptor agonist, on C-fibers, cholinergic fibers and mast cells in rabbit lungs in vitro.

    abstract::The pharmacological mechanisms involved in the interactions between C-fibers, cholinergic fibers and mast cells were investigated in tracheally perfused rabbit lungs by measuring the simultaneous release of substance P and histamine in lung effluents. The amounts of substance P and histamine released in lung superfusa...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/s0014-2999(99)00176-4

    authors: Nemmar A,Delaunois A,Beckers JF,Sulon J,Bloden S,Gustin P

    更新日期:1999-04-23 00:00:00

  • Aspirin and its pleiotropic application.

    abstract::Aspirin (acetylsalicylic acid), the oldest synthetic drug, was originally used as an anti-inflammatory medication. Being an irreversible inhibitor of COX (prostaglandin-endoperoxide synthase) enzymes that produce precursors for prostaglandins and thromboxanes, it has gradually found several other applications. Sometim...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章,评审

    doi:10.1016/j.ejphar.2019.172762

    authors: Hybiak J,Broniarek I,Kiryczyński G,Los LD,Rosik J,Machaj F,Sławiński H,Jankowska K,Urasińska E

    更新日期:2020-01-05 00:00:00

  • Gαi and Gβγ subunits have opposing effects on dexmedetomidine-induced sedation.

    abstract::Dexmedetomidine (DMED) is a potent and highly selective α2-adrenergic receptor agonist and is widely used for short-term sedation. However, the mechanism of DMED-induced sedation has not been deciphered. In the present study, we investigated the mechanism of Gαi and Gβγ subunits on DMED-induced sedation. An ED50 of DM...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/j.ejphar.2018.05.002

    authors: Liu M,Yang Y,Tan B,Li Y,Zhou P,Su R

    更新日期:2018-07-15 00:00:00

  • Receptor affinity and pharmacological potency of a series of narcotic analgesic, anti-diarrheal and neuroleptic drugs.

    abstract::A series of 26 drugs was tested for in vitro binding to opiate receptors in the presence and absence of 0.1 M NaCl. The results were correlated with assays for in vivo pharmacological potency. Highly significant correlation was found between binding in the presence and absence of sodium ions and analgesic potency. For...

    journal_title:European journal of pharmacology

    pub_type: 杂志文章

    doi:10.1016/0014-2999(77)90334-x

    authors: Stahl KD,van Bever W,Janssen P,Simon EJ

    更新日期:1977-12-01 00:00:00