Abstract:
AIM:To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. METHODS:Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation. RESULTS:Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture. CONCLUSION:The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Nyamath P,Alvi A,Habeeb A,Khosla S,Khan AA,Habibullah CMdoi
10.3748/wjg.v13.i16.2319subject
Has Abstractpub_date
2007-04-28 00:00:00pages
2319-23issue
16eissn
1007-9327issn
2219-2840journal_volume
13pub_type
杂志文章abstract:AIM:To assess the incidence of infantile colic and its association with variable predictors in infants born in a community maternity hospital, Tehran, Iran. METHODS:In this prospective cohort study, mothers who gave birth to live newborns between February 21 and March 20, 2003 at the hospital were invited to join to t...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.4662
更新日期:2008-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i5.445
更新日期:2012-02-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 社论,评审
doi:10.3748/wjg.v12.i25.3953
更新日期:2006-07-07 00:00:00
abstract:AIM:To examine the accuracy of the aspartate aminotransferase (AST)/Platelet Ratio Index (APRI) and FIB-4, in predicting longitudinal changes in liver histology in hepatitis C virus (HCV) patients. METHODS:Patients that underwent repeat liver biopsies at least 1 year apart from 1999 to 2007 were identified. Liver fibr...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i45.5710
更新日期:2010-12-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i42.6656
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pub_type: 杂志文章,评审
doi:10.3748/wjg.v23.i45.7945
更新日期:2017-12-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.45.6104
更新日期:2007-12-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v12.i30.4794
更新日期:2006-08-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i7.1060
更新日期:2007-02-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i34.10045
更新日期:2015-09-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v17.i36.4067
更新日期:2011-09-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v21.i41.11748
更新日期:2015-11-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i38.14076
更新日期:2014-10-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i36.5592
更新日期:2005-09-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
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更新日期:2012-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3748/wjg.v20.i16.4681
更新日期:2014-04-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v18.i46.6729
更新日期:2012-12-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v26.i22.2931
更新日期:2020-06-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i34.5412
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
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更新日期:2014-05-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i21.2623
更新日期:2011-06-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v8.i2.294
更新日期:2002-04-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i38.6031
更新日期:2005-10-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v19.i13.2019
更新日期:2013-04-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v25.i38.5732
更新日期:2019-10-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v9.i9.2078
更新日期:2003-09-01 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i1.326
更新日期:2014-01-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v12.i20.3265
更新日期:2006-05-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v17.i41.4554
更新日期:2011-11-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v10.i12.1699
更新日期:2004-06-15 00:00:00