Abstract:
BACKGROUND:It has recently become apparent that the lymph pump is an electrical entity that rivals the heart in complexity. Many interesting currents have been demonstrated by voltage clamping isolated lymphatic smooth muscle cells, but until now the role of these currents in the intact syncitium has not been studied. METHODS AND RESULTS:Intracellular microelectrode recordings were made from smooth muscle of sheep mesenteric lymphatics to investigate the electrophysiological basis of lymphatic pumping. Approximately 50% of the vessels exhibited spontaneous electrical activity, varying from regular oscillations in membrane potential to spike complexes. Spike complexes generally consisted of one or more action potentials superimposed on a slower depolarization or 'plateau' phase and were often preceded by a slow diastolic depolarization or 'pre-potential'. Norepinephrine (5 microM) induced depolarizing events in quiescent preparations. Both agonist-induced oscillations and spike complexes were attenuated or completely abolished by 2-aminoethoxydiphenyl borate (2-APB); 10-100 microM). Cesium (1 mM) reduced the frequency of spontaneous firing by approximately 30% by flattening the pre-potential phase. In addition to having a negative inotropic effect, 10 mM Cs(+) also caused gradual membrane depolarization and prolonged the plateau. 1 microM nifedipine abolished spontaneous events while tetrodotoxin (TTX; 0.5-1 muM) decreased the amplitude and maximum dV/dt of the spike upstroke or stopped activity completely. Spontaneously active segments of lymphatic vessel were inhibited by the chloride channel blocker, anthracene-9-carboxylic acid (9-AC; 250 microM - 1 mM) suggesting that I(Cl(Ca)) plays a significant role in the generation of spontaneous activity in this tissue. Penitrem-A (0.1 microM) did not affect resting membrane potential but increased action potential amplitude and prolonged the plateau, suggesting that calcium-activated potassium current does not make a significant contribution to resting membrane conductance but is important in membrane repolarization following calcium influx during the action potential. In contrast 4-aminopyridine (4-AP; 5 microM) caused significant membrane depolarization, suggesting the existence of an active 4-AP-sensitive current at rest. CONCLUSIONS:These results demonstrate that the currents found in isolated voltage-clamped cells from sheep mesenteric lymphatics do play a significant role in the shaping of spontaneous electrical activity of the intact syncitium.
journal_name
Lymphat Res Bioljournal_title
Lymphatic research and biologyauthors
Beckett EA,Hollywood MA,Thornbury KD,McHale NGdoi
10.1089/lrb.2007.5104subject
Has Abstractpub_date
2007-01-01 00:00:00pages
29-43issue
1eissn
1539-6851issn
1557-8585journal_volume
5pub_type
杂志文章abstract:BACKGROUND:Lymphedema of the arm and/or hand is a well-established complication of breast cancer therapy. The objective of the study was to determine the interest of auto-adjustable MOBIDERM® Autofit night arm sleeve in controlling edema rebound effect in breast cancer-related lymphedema patients just after a successfu...
journal_title:Lymphatic research and biology
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Lymphatic research and biology
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更新日期:2006-04-01 00:00:00
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journal_title:Lymphatic research and biology
pub_type: 杂志文章
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abstract:BACKGROUND:Analysis of the innervation pattern of the thoracic duct in young and elderly human subjects has been performed. The subdivision of the vessels in cervical and lumbar region were taken in consideration. METHODS AND RESULTS:Immunostaining for general nerve fibers with a PGP 9.5 marker disclosed a diffuse inn...
journal_title:Lymphatic research and biology
pub_type: 杂志文章
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abstract:BACKGROUND:Lymphedema of limbs affects a large mass of tissues. Pathological changes develop in skin and subcutaneous tissue. Bacterial retention in edema fluid is followed by chronic inflammatory reaction. The question arises whether the chronic processes affecting a large mass of limb tissues are reflected in the ser...
journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
pub_type: 杂志文章,评审
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doi:10.1089/lrb.2018.0065
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journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
pub_type: 杂志文章
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journal_title:Lymphatic research and biology
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更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Lymphedema is the abnormal accumulation of protein-rich fluid in the interstitial space. Primary lymphedema is a rare genetic condition with both autosomal dominant and autosomal recessive modes of inheritance. Three genes, FLT4 (VEGFR3), FOXC2, and SOX18 cause varying forms of primary lymphedema. In industr...
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journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
pub_type: 杂志文章
doi:10.1089/lrb.2011.0015
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journal_title:Lymphatic research and biology
pub_type: 杂志文章
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abstract::Hemangiomas of infancy are very common tumors, but they are heterogeneous in their behavior. A small, but significant, subset causes medical complications or permanent disfigurement, but due to their heterogeneity, there is no appropriate "one size fits all" approach to management. In addition, the rapid evolution of ...
journal_title:Lymphatic research and biology
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pub_type: 杂志文章,多中心研究
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journal_title:Lymphatic research and biology
pub_type: 杂志文章,评审
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journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
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journal_title:Lymphatic research and biology
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