Abstract:
:Protein nitration occurs as a result of oxidative stress induced by reactive oxygen (ROS) and reactive nitrogen species (RNS). Therefore, protein nitration serves as a hallmark for protein oxidation in vivo. We have previously reported on age dependent protein nitration in cardiac tissue of Fisher 344 BN-F1 rats analyzed by two-dimensional gel electrophoresis; however, only one specific nitration site was identified [Kanski, J., Behring, A., Pelling, J., Schöneich, C., 2005a. Proteomic identification of 3-nitrotyrosine-containing rat cardiac proteins: effects of biological aging. Am. J. Physiol. Heart Circ. Physiol. 288, H371-381]. In the present report, we used solution phase isoelectric focusing (IEF) followed by nanoHPLC-ESI-MS/MS that allowed us to obtain good MS/MS data to identify specific sites of protein nitration in cardiac tissue. As expected, more nitrated proteins were detected in cardiac tissue of old rats, including myosin heavy chain, neurofibromin, tropomyosin and nebulin-related anchoring protein. The post-translational modification of these cytoskeletal proteins may provide some rationale for the age-dependent functional decline of the heart.
journal_name
Exp Gerontoljournal_title
Experimental gerontologyauthors
Hong SJ,Gokulrangan G,Schöneich Cdoi
10.1016/j.exger.2007.03.005subject
Has Abstractpub_date
2007-07-01 00:00:00pages
639-51issue
7eissn
0531-5565issn
1873-6815pii
S0531-5565(07)00059-9journal_volume
42pub_type
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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journal_title:Experimental gerontology
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2016.03.021
更新日期:2016-07-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
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journal_title:Experimental gerontology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
doi:10.1016/j.exger.2010.01.003
更新日期:2010-08-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2006.01.011
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pub_type: 杂志文章
doi:10.1016/j.exger.2010.06.004
更新日期:2011-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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更新日期:1998-11-01 00:00:00
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journal_title:Experimental gerontology
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doi:10.1016/j.exger.2009.06.008
更新日期:2009-09-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2004.12.002
更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0531-5565(96)00031-9
更新日期:1996-09-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,多中心研究
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pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Experimental gerontology
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更新日期:2020-04-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(90)90059-b
更新日期:1990-01-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.01.001
更新日期:2017-03-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
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更新日期:2011-02-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2017.08.021
更新日期:2018-02-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/j.exger.2015.04.012
更新日期:2015-07-01 00:00:00
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journal_title:Experimental gerontology
pub_type: 杂志文章,评审
doi:10.1016/j.exger.2012.07.010
更新日期:2013-07-01 00:00:00
abstract::Effects of glucagon and epinephrine stimulation on urea synthesis from lactate, pyruvate, NH4Cl, and ornithine in isolated rat hepatocytes from young and old rats were compared. It was found that the production of urea from those substrates was almost the same in both age groups. The concentrations of hormones that ca...
journal_title:Experimental gerontology
pub_type: 杂志文章
doi:10.1016/0531-5565(85)90052-x
更新日期:1985-01-01 00:00:00