Rational design of a selective antagonist of epsilon protein kinase C derived from the selective allosteric agonist, pseudo-RACK peptide.

abstract：:The effect of alpha1-adrenergic stimulation on L-type Ca2+ current (ICa,L) in adult rat ventricular myocytes was investigated using three different methods of current recording. During conventional whole-cell recordings with 5 mm-BAPTA included in the pipette solution, phenylephrine (20 microM) did not increase ICa,L ...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang S,Hiraoka M,Hirano Y

更新日期：1998-10-01 00:00:00

abstract：:Understanding the process of Ca(2+)/Mg(2+)exchange during muscle excitation and relaxation is fundamental to elucidating the mechanism of Ca(2+)-regulated muscle contraction. During the resting phase, the C-domain of cardiac troponin C may be occupied by either Ca(2+)or Mg(2+). Here, complexes of recombinant cardiac t...

journal_title：Journal of molecular and cellular cardiology

authors： Finley N,Dvoretsky A,Rosevear PR

更新日期：2000-08-01 00:00:00

abstract：:Preconditioning of hearts with the α(1)-adrenoceptor agonist phenylephrine decreases infarct size and increases the functional recovery of the heart following ischaemia-reperfusion. However, the cellular mechanisms responsible for this protection are not known. We investigated the role of protein kinase C ε and δ (PKC...

journal_title：Journal of molecular and cellular cardiology

authors： Turrell HE,Rodrigo GC,Norman RI,Dickens M,Standen NB

更新日期：2011-09-01 00:00:00

abstract：:Defective sarcoplasmic reticulum (SR) Ca2+ handling is evident in cardiomyopathy and may be mediated by selective dysregulation of SR Ca2+ handling proteins. To assess whether regulation of SR Ca2+ release may vary regionally within the normal and diseased heart, left ventricular transmural expression and activity of ...

journal_title：Journal of molecular and cellular cardiology

authors： Currie S,Quinn FR,Sayeed RA,Duncan AM,Kettlewell S,Smith GL

更新日期：2005-08-01 00:00:00

abstract：:To determine whether high free fatty acids (FFA) could affect the anti-contractile properties of perivascular adipose tissue (PVAT) in rat aortas. Wistar rats were divided into normal, obesity and fenofibrate groups and fed a normal, high-fat, and high-fat plus fenofibrate diet, respectively. Thoracic aortas with or w...

journal_title：Journal of molecular and cellular cardiology

authors： Sun X,Hou N,Han F,Guo Y,Hui Z,Du G,Zhang Y

更新日期：2013-10-01 00:00:00

abstract：:We measured mitochondrial NADH autofluorescence or Ca(2+) using Rhod-2, simultaneously with cell shortening in isolated guinea-pig ventricular myocytes. When both frequency and amplitude of twitch shortening (work intensity) were increased by raising stimulus frequency in incremental steps from 0.1 to 3.3 Hz, the stea...

journal_title：Journal of molecular and cellular cardiology

authors： Jo H,Noma A,Matsuoka S

更新日期：2006-03-01 00:00:00

abstract：:Thoracic aortic aneurysm/dissection (TAAD) is characterized by excessive smooth muscle cell (SMC) loss, extracellular matrix (ECM) degradation and inflammation. However, the mechanism whereby signaling leads to SMC loss is unclear. We used senescence-associated (SA)-β-gal staining and analysis of expression of senesce...

journal_title：Journal of molecular and cellular cardiology

authors： Zhang WM,Liu Y,Li TT,Piao CM,Liu O,Liu JL,Qi YF,Jia LX,Du J

更新日期：2016-10-01 00:00:00

abstract：:Mutations causing familial hypertrophic cardiomyopathy (HCM) have been described in at least 11 genes encoding cardiac sarcomeric proteins. In this study, three previously unknown deletions have been identified in the human cardiac genes coding for beta-myosin heavy chain (MYH7 on chromosome 14) and myosin-binding pro...

journal_title：Journal of molecular and cellular cardiology

authors： Waldmüller S,Sakthivel S,Saadi AV,Selignow C,Rakesh PG,Golubenko M,Joseph PK,Padmakumar R,Richard P,Schwartz K,Tharakan JM,Rajamanickam C,Vosberg HP

更新日期：2003-06-01 00:00:00

abstract：:The myocardial extracellular matrix (ECM) is composed of three important constituents: (1) fibrillar collagen, (2) a basement membrane, and (3) proteoglycans. Structural or compositional changes in these ECM components may affect left ventricular (LV) function as well as influence overall LV geometry. Accordingly, thi...

journal_title：Journal of molecular and cellular cardiology

authors： Spinale FG,Zellner JL,Johnson WS,Eble DM,Munyer PD

更新日期：1996-08-01 00:00:00

abstract：:The purposes of this study were to: (1) assess myocardial pyruvate dehydrogenase (PDH) activity and substrate exchange under well-perfused and ischemic conditions; (2) determine the metabolic effects of an intra-coronary infusion of the PDH activator, dichloroacetate (DCA); and (3) measure the effects of ischemia and ...

journal_title：Journal of molecular and cellular cardiology

authors： Stanley WC,Hernandez LA,Spires D,Bringas J,Wallace S,McCormack JG

更新日期：1996-05-01 00:00:00

abstract：:To understand better the pathophysiological roles of the vagal efferent system in ischemic heart diseases, we examined endogenous acetylcholine (ACh) release in the myocardium in vivo. Acute myocardial ischemia was induced in anesthetized cats by a 60-min occlusion of the left anterior descending coronary artery (LAD)...

journal_title：Journal of molecular and cellular cardiology

authors： Kawada T,Yamazaki T,Akiyama T,Sato T,Shishido T,Inagaki M,Takaki H,Sugimachi M,Sunagawa K

更新日期：2000-03-01 00:00:00

abstract：:Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined muri...

journal_title：Journal of molecular and cellular cardiology

authors： Lenzo JC,Fairweather D,Cull V,Shellam GR,James Lawson CM

更新日期：2002-06-01 00:00:00

abstract：:Our previous studies showed that homocysteine (Hcy) reduces endothelial progenitor cell (EPC) numbers and impairs functional activity. However, the mechanisms by which Hcy reduces EPCs numbers and activity remain to be determined. Recent studies have demonstrated that reduced EPCs numbers and activity was associated w...

journal_title：Journal of molecular and cellular cardiology

authors： Zhu JH,Chen JZ,Wang XX,Xie XD,Sun J,Zhang FR

更新日期：2006-05-01 00:00:00

abstract：:We investigated the role of neuropeptide Y (NPY), abundant in the myocardial sympathetic nervous system and endothelial cells, in angiogenesis during chronic myocardial ischemia. Adult male Yorkshire swine underwent ameroid constrictor placement on the proximal left circumflex coronary artery. After 3 weeks, an osmoti...

journal_title：Journal of molecular and cellular cardiology

authors： Robich MP,Matyal R,Chu LM,Feng J,Xu SH,Laham RJ,Hess PE,Bianchi C,Sellke FW

更新日期：2010-12-01 00:00:00

abstract：:In cardiomyocytes, Ca(2+) plays a central role in governing both contraction and signaling events that regulate gene expression. Current evidence indicates that discrimination between these two critical functions is achieved by segregating Ca(2+) within subcellular microdomains: transcription is regulated by Ca(2+) re...

journal_title：Journal of molecular and cellular cardiology

authors： Ibarra C,Vicencio JM,Varas-Godoy M,Jaimovich E,Rothermel BA,Uhlén P,Hill JA,Lavandero S

更新日期：2014-10-01 00:00:00

abstract：:The modulating effects of Ca2+ on single K+ channel currents in canine heart sarcoplasmic reticulum were studied using a planar lipid bilayer technique. The open-state probability and the unitary open-state current both decreased gradually as the Ca2+ concentration was reduced from pCa 3 to pCa 7.5. Each single-channe...

journal_title：Journal of molecular and cellular cardiology

authors： Uehara A,Yasukochi M,Imanaga I

更新日期：1994-02-01 00:00:00

abstract：:In the mouse, genetic reduction in the Na(+), K(+)-ATPase alpha1 or alpha2 isoforms results in different functional phenotypes: heterozygous alpha2 isolated hearts are hypercontractile, whereas heterozygous alpha1 hearts are hypocontractile. We examined Na(+)/Ca(2+) exchange (NCX) currents in voltage clamped myocytes ...

journal_title：Journal of molecular and cellular cardiology

authors： Yamamoto T,Su Z,Moseley AE,Kadono T,Zhang J,Cougnon M,Li F,Lingrel JB,Barry WH

更新日期：2005-07-01 00:00:00

abstract：:PICOT (PKC-interacting cousin of thioredoxin) was previously shown to inhibit the development of cardiac hypertrophy, concomitant with an increase in cardiomyocyte contractility. To explore the physiological function of PICOT in the hearts, we generated a PICOT-deficient mouse line by using a gene trap approach. PICOT...

journal_title：Journal of molecular and cellular cardiology

authors： Cha H,Kim JM,Oh JG,Jeong MH,Park CS,Park J,Jeong HJ,Park BK,Lee YH,Jeong D,Yang DK,Bernecker OY,Kim DH,Hajjar RJ,Park WJ

更新日期：2008-12-01 00:00:00

abstract：:(±)-Sodium5-bromo-2-(α-hydroxypentyl) benzoate (brand name: brozopine, BZP, 1a), derived from L-3-n-butylphthalide (L-NBP), has been reported to protect the brain from stoke and has been approved by CFDA in Phase I-II clinical trials. However, it remains to be investigated whether 1a may exhibit any cardioprotective e...

journal_title：Journal of molecular and cellular cardiology

authors： Xiao Y,Song C,Lin Q,Shi X,Yu W,Huang X,Wang H,Chen Y,Wang R,Geng X,Qin M,Hu K,Fan Y,Qiao Y,Gao E,Zhao W,Chang J

更新日期：2019-10-01 00:00:00

abstract：:Oxygen deprivation for prolonged periods leads to cardiac cell death and ventricular dysfunction. The ability to prevent myocardial cell death would be of significant therapeutic value in maintaining cardiac function after injury. While caspases have been suggested to play a critical role in apoptosis, their involveme...

journal_title：Journal of molecular and cellular cardiology

authors： de Moissac D,Gurevich RM,Zheng H,Singal PK,Kirshenbaum LA

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Rodent cardiomyocytes (CM) undergo mitotic arrest and decline of mononucleated-diploid population post-birth, which are implicated in neonatal loss of heart regenerative potential. However, the dynamics of postnatal CM maturation are largely unknown in swine, despite a similar neonatal cardiac regenerative c...

journal_title：Journal of molecular and cellular cardiology

authors： Velayutham N,Alfieri CM,Agnew EJ,Riggs KW,Baker RS,Ponny SR,Zafar F,Yutzey KE

更新日期：2020-09-01 00:00:00

abstract：:To explore whether CaMKII-dependent phosphorylation events mediate reperfusion arrhythmias, Langendorff perfused hearts were submitted to global ischemia/reperfusion. Epicardial monophasic or transmembrane action potentials and contractility were recorded. In rat hearts, reperfusion significantly increased the number ...

journal_title：Journal of molecular and cellular cardiology

authors： Said M,Becerra R,Valverde CA,Kaetzel MA,Dedman JR,Mundiña-Weilenmann C,Wehrens XH,Vittone L,Mattiazzi A

更新日期：2011-12-01 00:00:00

abstract：:Cardiac fibrosis is implicated in numerous physiologic and pathologic conditions, including scar formation, heart failure and cardiac arrhythmias. However the specific cells and signaling pathways mediating this process are poorly understood. Lysine acetylation of nucleosomal histone tails is an important mechanism fo...

journal_title：Journal of molecular and cellular cardiology

authors： Schuetze KB,McKinsey TA,Long CS

更新日期：2014-05-01 00:00:00

abstract：:The key information processing units within gene regulatory networks are enhancers. Enhancer activity is associated with the production of tissue-specific noncoding RNAs, yet the existence of such transcripts during cardiac development has not been established. Using an integrated genomic approach, we demonstrate that...

journal_title：Journal of molecular and cellular cardiology

authors： Ounzain S,Pezzuto I,Micheletti R,Burdet F,Sheta R,Nemir M,Gonzales C,Sarre A,Alexanian M,Blow MJ,May D,Johnson R,Dauvillier J,Pennacchio LA,Pedrazzini T

更新日期：2014-11-01 00:00:00

abstract：:The AT1 receptor (AT1R) mediates the manifold actions of angiotensin II in the cardiovascular system. This study probed the molecular mechanisms that link altered redox status to AT1R expression in cardiac fibroblasts. Real-time PCR and western blot analysis showed that H2O2 enhances AT1R mRNA and protein expression v...

journal_title：Journal of molecular and cellular cardiology

authors： Anupama V,George M,Dhanesh SB,Chandran A,James J,Shivakumar K

更新日期：2016-08-01 00:00:00

abstract：:During pressure overload hypertrophy, selective changes in cardiac gene expression occur that regulate growth and modify the structural and functional properties of the myocardium. To determine the role of translational mechanisms, a murine model of transverse aortic constriction was used to screen a set of specified ...

journal_title：Journal of molecular and cellular cardiology

authors： Spruill LS,Baicu CF,Zile MR,McDermott PJ

更新日期：2008-01-01 00:00:00

abstract：:Pathological cardiac remodeling, characterized by excessive deposition of extracellular matrix proteins and cardiac hypertrophy, leads to the development of heart failure. Meprin α (Mep1a), a zinc metalloprotease, previously reported to participate in the regulation of inflammatory response and fibrosis, may also cont...

journal_title：Journal of molecular and cellular cardiology

authors： Ge W,Hou C,Zhang W,Guo X,Gao P,Song X,Gao R,Liu Y,Guo W,Li B,Zhao H,Wang J

更新日期：2020-12-08 00:00:00

abstract：:The concept of metabolic protection of the ischemic myocardium is in constant evolution and has recently been supported by clinical studies. Historically, enhanced glucose metabolism and glycolysis were proposed as anti-ischemic cardioprotection. This hypothesis is supported by the sub-cellular linkage between key gly...

journal_title：Journal of molecular and cellular cardiology

authors： Opie LH,Sack MN

更新日期：2002-09-01 00:00:00

abstract：:We have recently shown that norepinephrine (NE) pretreatment attenuates Ca2+ overloading in cardiac rat trabeculae during metabolic inhibition (MI) with NaCN (2 mmol/l), and improves contractile recovery during a subsequent recovery period (RP). In the present study, we investigated the effects of the continuous prese...

journal_title：Journal of molecular and cellular cardiology

authors： Musters RJ,van der Meulen ET,van der Laarse WJ,van Hardeveld C

更新日期：1998-02-01 00:00:00

abstract：:The sympathetic nervous system is the main stimulator of cardiac function. While acute activation of the β-adrenoceptors exerts positive inotropic and lusitropic effects by increasing cAMP and Ca2+, chronically enhanced sympathetic tone with changed β-adrenergic signaling leads to alterations of gene expression and re...

journal_title：Journal of molecular and cellular cardiology

authors： Morhenn K,Quentin T,Wichmann H,Steinmetz M,Prondzynski M,Söhren KD,Christ T,Geertz B,Schröder S,Schöndube FA,Hasenfuss G,Schlossarek S,Zimmermann WH,Carrier L,Eschenhagen T,Cardinaux JR,Lutz S,Oetjen E

更新日期：2019-02-01 00:00:00