Abstract:
BACKGROUND:The worldwide increasing incidence of allergic diseases requires the development of new, efficient vaccination strategies, the only curative treatment with a long-lasting effect. Current allergen-specific immunotherapy protocols suffer from limited efficacy and a long treatment time. METHODS:We engineered modular antigen translocating (MAT) molecules for intracellular targeting of allergens to the major histocompatibility class-II (MHC-II) presentation pathway to enhance antigen presentation. MAT-fusions were evaluated for their ability to localize intracellularly, to induce proliferation, and for their influence on cytokine patterns in peripheral blood mononuclear cells (PBMCs) cultures. RESULTS:We show that MAT-allergen fusions are able to rapidly translocate into the cytoplasm of PBMCs, whereas naked recombinant allergens are only marginally taken up. MAT vaccines accumulate intracellularly and induce strong proliferation of PBMC cultures at concentrations 10-100 times lower than the corresponding naked allergens, indicating an enhanced presentation through the MHC-II presentation pathway. In PBMC cultures of allergic donors, MAT vaccines induce a cytokine shift from a T(H)2 to a T(H)1 profile, resulting in a stronger and earlier secretion of INF-gamma and Interleukin (IL)-10, and a decreased secretion of IL-4, IL-5 and IL-2, compared with those induced by the corresponding recombinant allergens. CONCLUSION:Modular antigen translocation vaccines induce strong proliferation responses in PBMC cultures at low concentration and induce a T(H)1/T(H)2 shift in the cytokine profile, reflecting those reported to occur in successfully desensitized allergic patients. Therefore, MAT molecules represent promising lead compounds for the development of potent allergy vaccines.
journal_name
Allergyjournal_title
Allergyauthors
Crameri R,Flückiger S,Daigle I,Kündig T,Rhyner Cdoi
10.1111/j.1398-9995.2006.01292.xsubject
Has Abstractpub_date
2007-02-01 00:00:00pages
197-206issue
2eissn
0105-4538issn
1398-9995pii
ALL1292journal_volume
62pub_type
杂志文章相关文献
ALLERGY文献大全abstract:BACKGROUND:Allergy diagnosis by determination of allergen-specific IgE is complicated by clinically irrelevant IgE, of which the most prominent example is IgE against cross-reactive carbohydrate determinants (CCDs) that occur on allergens from plants and insects. Therefore, CCDs cause numerous false-positive results. I...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12229
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND:It is commonly acknowledged that asthma is exacerbated by viral infections. On the other hand, basophil infiltration of lung tissues has been evidenced postmortem in cases of fatal disease, raising the question of a possible link between these two observations. OBJECTIVES:Herein, we addressed the relationsh...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12151
更新日期:2013-06-01 00:00:00
abstract::Food allergies have become a significant heath burden as prevalence continues to rise, affecting 6%-13% of the global population. In the absence of drugs approved by regulatory agencies, the current standard of care remains avoidance of allergenic foods and management of acute allergic reactions with antihistamines an...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/all.14116
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Bakery workers may develop IgE-mediated allergy to liquid and aerosolized hen's egg proteins that are commonly used in the baking and confectionery industries. METHODS:We studied four bakery workers who had work-related allergic respiratory symptoms upon exposure to egg aerosols. The causative role of egg p...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2003.00201.x
更新日期:2003-07-01 00:00:00
abstract::Using monoclonal anti-human IgE antibodies recognizing the D epsilon 1 or D epsilon 2 epitope, we have developed a sandwich radioimmunoassay (RIA) to determine IgE in human cell cultures. With the help of this assay, various methods of measuring an actual de novo IgE synthesis were compared. It was necessary to subtra...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1986.tb00327.x
更新日期:1986-08-01 00:00:00
abstract::The increase in prevalence and burden of atopic diseases, i.e. eczema, rhinitis, and asthma over the past decades was paralleled by a worldwide increase in attention-deficit/hyperactivity disorder (ADHD) diagnoses. We systematically reviewed epidemiologic studies investigating the relationship between atopic diseases ...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2010.02449.x
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Dupilumab, a fully human monoclonal antibody that binds IL-4Rα and inhibits signaling of both IL-4 and IL-13, has shown efficacy across multiple diseases with underlying type 2 signatures and is approved for treatment of asthma, atopic dermatitis, and chronic sinusitis with nasal polyposis. We sought to prov...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14151
更新日期:2020-05-01 00:00:00
abstract::Cat flea sensitivity is considered one of the most important skin diseases in cats and dogs. Cat fleas, however, are also a growing allergen problem for humans. Cat flea-specific IgE antibodies were studied in serum samples from 70 patients with suspected cat allergy, using RAST-based techniques and the nitrocellulose...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1987.tb02196.x
更新日期:1987-04-01 00:00:00
abstract:BACKGROUND:Itch is a major symptom of allergic skin disease. Acupuncture has been shown to exhibit a significant effect on histamine-induced itch in healthy volunteers. We investigated the effect of acupuncture on type I hypersensitivity itch and skin reaction in a double-blind, randomized, placebo-controlled, crossove...
journal_title:Allergy
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1398-9995.2009.02284.x
更新日期:2010-07-01 00:00:00
abstract::In a double-blind double-placebo study 10 asthmatics exercised on a treadmill after treatment for 1 week with placebo, or ketotifen 1 mg twice daily, or SCG 10 mg four times daily. The post-exercise percentage fall in FEV1 after SCG but not after ketotifen was significantly smaller than after placebo. A 1-week treatme...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1398-9995.1987.tb02215.x
更新日期:1987-05-01 00:00:00
abstract:BACKGROUND:Asthma is a complex genetic disorder characterized by chronic inflammation in the airways. Identification of genetic risk factors for asthma has been complicated due to genetic heterogeneity and influence from environmental risk factors. Despite the fact that multiple genetic linkage studies have been carrie...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2008.01699.x
更新日期:2008-09-01 00:00:00
abstract::All eight production workers from a small trout-processing factory were studied because of respiratory or rhinitis symptoms when working next to the automatic gutting machine. Seven of the eight had positive histamine provocation tests. Four patients had elevated total IgE levels while one had a slightly elevated eosi...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1989.tb00455.x
更新日期:1989-07-01 00:00:00
abstract::Two methods of relating daily pollen counts to patients' symptom scores in clinical trials of hyposensitisation in hay fever are described. The problems involved in comparing results from different centres and years of a trial are discussed with particular reference to symptoms which are not pollen related. The Normal...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1984.tb01969.x
更新日期:1984-08-01 00:00:00
abstract:BACKGROUND:IgE-independent (pseudoallergic) reactions to food and food ingredients are common in a subgroup of adult patients with chronic urticaria, who have daily spontaneous occurrence of wheals. However, for children with chronic urticaria (duration longer than 6 weeks, no physical influence), no data on the import...
journal_title:Allergy
pub_type: 临床试验,杂志文章
doi:10.1111/j.1398-9995.1998.tb03817.x
更新日期:1998-11-01 00:00:00
abstract::The efficacy and tolerability of intranasal azelastine (0.14 mg/nostril twice daily) and oral terfenadine (60 mg twice daily) were compared under double-blind conditions in two 6-week, multicenter, parallel-group studies, including 167 patients suffering from seasonal and 52 patients suffering from perennial allergic ...
journal_title:Allergy
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1398-9995.1994.tb00818.x
更新日期:1994-03-01 00:00:00
abstract::We describe a case of contact allergic sensitization to budesonide in a nasal spray used in the treatment of allergic rhinitis. No cross-sensitivity to other topical corticosteroids was found. ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1992.tb02392.x
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:Monocyte (Mo) subsets exhibiting distinct phenotypic and functional properties identified in peripheral blood are assumed to be under the control of soluble factors from their surrounding micromilieu. Atopic eczema/dermatitis syndrome (AEDS) is accompanied by humoral and cellular alterations among which an i...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2002.23737.x
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Patch testing is the standard clinical procedure to prove contact sensitization. It is a common practice to attach multiple patch tests at the same time. However, synchronous reactions to unrelated allergens may not be completely unassociated. If so, the reaction in a given test field might be influenced by ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2006.01212.x
更新日期:2006-12-01 00:00:00
abstract:BACKGROUND:Budesonide/formoterol (Symbicort) Maintenance and Reliever Therapy (SMART) is an effective asthma-management approach that treats symptoms with rapid increases in budesonide/formoterol. The cost-effectiveness of SMART vs higher fixed-dose budesonide/formoterol or salmeterol/fluticasone is unknown. METHODS:T...
journal_title:Allergy
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1398-9995.2007.01466.x
更新日期:2007-10-01 00:00:00
abstract::This study is an extended follow-up for 24 months of a 12-week trial to study the long-term clinical efficacy of low-dose inhaled budesonide (BUD) once or twice daily in children with mild asthma. A total of 122 children (mean age 9.7 years, girls/boys; 42/80) with mild asthma (FEV1 103.7% of predicted, reversibility ...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1034/j.1398-9995.2000.00661.x
更新日期:2000-08-01 00:00:00
abstract:BACKGROUND:The cysteinyl leukotrienes (cysLTs) play a key role in the pathophysiology of asthma. In addition to functioning as potent bronchoconstrictors, cysLTs contribute to airway inflammation through eosinophil and neutrophil chemotaxis, plasma exudation, and mucus secretion. We tested the activity of the dual cysL...
journal_title:Allergy
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/all.12987
更新日期:2016-12-01 00:00:00
abstract::CD4+ T effector lymphocytes are distinguished in different subsets on the basis of their patterns of cytokine secretion. Th1 cells, thank to IFN-γ production, are responsible for cell-mediated immunity against intracellular pathogens, Th2 cells, through the production of IL-4, provide some degree of protection against...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2011.02576.x
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Omalizumab, a recombinant monoclonal anti-immunoglobulin E (IgE) antibody, shows proven efficacy in the treatment of allergic diseases. A little is known about the immunological pathways affected by the decrease of circulating free IgE during omalizumab treatment. AIM OF THE STUDY:To investigate the immunol...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2006.01180.x
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND:Sensitization to atopens is an early phenomenon that overlaps with the onset of atopic dermatitis (AD) in infancy. Early epidermal barrier impairment may facilitate the epicutaneous penetration of atopens. OBJECTIVE:To correlate transepidermal water loss (TEWL) and aeroallergen sensitization in infants with...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2007.01556.x
更新日期:2008-02-01 00:00:00
abstract::Basophils are primarily associated with immunomodulatory functions in allergic diseases and parasitic infections. Recently, it has been demonstrated that both activated human and mouse basophils can form extracellular DNA traps (BETs) containing mitochondrial DNA and granule proteins. In this report, we provide eviden...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12662
更新日期:2015-09-01 00:00:00
abstract::The hygiene hypothesis states that a reduced exposure to allergens in early life is solely implicated in the growing propensity for allergy sensitization. Important elements of the hypothesis include helminth infection, exposure to endotoxins, exposure to pets and growing up on a farm. However, the hygiene hypothesis ...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2005.00854.x
更新日期:2005-01-01 00:00:00
abstract::In a study of 770 patients with allergic rhinitis, anamnestic data were collected by means of a questionnaire. 484 (63%) of the patients were women and 286 (37%) men. 45.5% of the patients had perennial symptoms, 33.5% had seasonal symptoms only, and 21% had predominantly seasonal but also some perennial symptoms. Mor...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1982.tb02317.x
更新日期:1982-08-01 00:00:00
abstract::Histamine is the primary mediator involved the pathophysiology of allergic rhinitis and chronic urticaria, and this explains the prominent role that histamine H(1)-receptor antagonists have in the treatment of these disorders. However, histamine is clearly not the only mediator involved in the inflammatory cascade. Th...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2008.01640.x
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Epicutaneous sensitization is an important route of immunization for allergens in atopic diseases; however, studies have also shown that application with protein on the intact skin induces antigen-specific tolerance. Langerhans cells (LCs) play an immunosuppressive role in several inflammatory skin diseases ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13813
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Dysphagia is the main symptom of adult eosinophilic esophagitis (EoE). We describe a novel syndrome, referred to as "food-induced immediate response of the esophagus" (FIRE), observed in EoE patients. METHODS:Food-induced immediate response of the esophagus is an unpleasant/painful sensation, unrelated to d...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14495
更新日期:2021-01-01 00:00:00