Heterogeneity of peripheral blood reticulocytes: a flow cytometric analysis with monoclonal antibody HAE9 and thiazole orange.

Abstract:

:The expression of a human erythroid cell surface antigen recognized by monoclonal antibody (mAB) HAE9 has been studied on peripheral blood reticulocytes by one- and two-color flow cytometry. Total reticulocyte count was determined using Thiazole Orange (TO) and flow cytometry. In normal individuals, 4.56% of reticulocytes were stained by FITC-labeled mAB HAE9. The correlation between reticulocyte percentage by TO and HAE9 staining was 0.828 (P less than 0.0001) in patients with hematocrits less than 0.25. A HAE9-positive reticulocyte percentage of 6-44% was observed when analyzed by two-color flow cytometry with TO and mAB HAE9. These findings, in conjunction with previous studies, suggest that mAB HAE9 recognizes an early, less differentiated population of peripheral blood reticulocytes. Enumeration of immature reticulocytes may be of clinical utility.

journal_name

Am J Hematol

authors

Mechetner EB,Sedmak DD,Barth RF

doi

10.1002/ajh.2830380110

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

61-3

issue

1

eissn

0361-8609

issn

1096-8652

journal_volume

38

pub_type

杂志文章
  • High-dose cytosine arabinoside for acute nonlymphocytic leukemia.

    abstract::Eighteen patients with acute nonlymphocytic leukemia (ANLL), aged 17-73 years, were treated with high-dose cytosine arabinoside (HD-Ara-C) using 3 g/m2 IV q 12 hours X 12 doses. Seven patients were treated for relapse and four (57%) obtained a complete remission with a median duration of 19.5 weeks. In nine patients, ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830160108

    authors: Cantin G,Brennan JK

    更新日期:1984-01-01 00:00:00

  • Association of single nucleotide polymorphisms in the thrombopoietin-receptor gene, but not the thrombopoietin gene, with differences in platelet count.

    abstract::Little is known about the mechanisms explaining the wide variation in platelet counts (PLT) and other hematologic parameters in humans. We previously showed that the sex-based difference in hematocrit was associated with nucleotide variation in the erythropoietin receptor gene (EPOR). We sought to identify new polymor...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.20095

    authors: Zeng SM,Murray JC,Widness JA,Strauss RG,Yankowitz J

    更新日期:2004-09-01 00:00:00

  • A case of chronic aneosinocytosis.

    abstract::A 59-year-old woman, after complete recovery from an episode of drug-induced agranulocytosis, was found to sustain a chronic absence of recognizable mature and immature eosinophils in blood and bone marrow during a follow-up period of 8 years. Her bone marrow and peripheral blood cells cultured in vitro were able to p...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830120211

    authors: Telerman A,Amson RB,Delforge A,Collard E,Stryckmans PA

    更新日期:1982-04-01 00:00:00

  • Acquired factor XI inhibitors in congenitally deficient patients.

    abstract::Four factor XI (F XI)-deficient patients are described, all of whom formed circulating anticoagulants against F X1. In the three most severely affected patients (F XI 0%-6% activity), the anticoagulant appeared to have been stimulated by plasma infusion. However, in the milder case (25% F XI activity), no infusion had...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830260405

    authors: Schnall SF,Duffy TP,Clyne LP

    更新日期:1987-12-01 00:00:00

  • Studies of marmoset megakaryocytopoiesis in vitro.

    abstract::Liquid culture of the mononuclear fraction of the blood of marmosets without any added stimulator resulted in the appearance of megakaryocytes. These cells could not be identified by light microscopy or by indirect immunofluorescence using antiplatelet antiserum. Ultrastructural identification was made using platelet ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830080108

    authors: Burstein SA,Breton-Gorius J,Harker LA

    更新日期:1980-01-01 00:00:00

  • Juvenile features in adult-type chronic granulocytic leukemia.

    abstract::Philadelphia chromosome (Ph1) positive, or "adult-type," chronic granulocytic leukemia (CGL) differs in many clinical and laboratory aspects from PH1-negative, or "juvenile," CGL. Because of these differences, the disorders have been considered two distinct disease entities. We present a child with Ph1-positive CGL wh...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830160114

    authors: Gay JC,Dessypris EN,Roloff JS,Lukens JN

    更新日期:1984-01-01 00:00:00

  • Autoimmune hemolytic anemia.

    abstract::Red blood cell (RBC) autoantibodies are a relatively uncommon cause of anemia. However, autoimmune hemolytic anemia (AIHA) must be considered in the differential diagnosis of hemolytic anemias, especially if the patient has a concomitant lymphoproliferative disorder, autoimmune disease, or viral or mycoplasmal infecti...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.10062

    authors: Gehrs BC,Friedberg RC

    更新日期:2002-04-01 00:00:00

  • Lymphocyte phenotype and function in chronically transfused children with sickle cell disease.

    abstract::Immunologic studies were performed on mononuclear cells from ten chronically transfused children with sickle cell disease, and the results were compared with those from five other groups: 21 sickle cell patients who were not receiving regular transfusions, 6 chronically transfused children with other forms of refracto...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830200106

    authors: Wang W,Herrod H,Presbury G,Wilimas J

    更新日期:1985-09-01 00:00:00

  • Serum-free culture of human hemopoietic progenitors in attenuated culture media.

    abstract::To elucidate the precise mechanisms of molecular and cellular regulation of hemopoiesis, it is necessary to develop a chemically defined culture assay for purified hemopoietic progenitors. To approach this long-term goal, we attempted to develop a serum-free culture system for enriched human progenitors that permits e...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830280404

    authors: Sonoda Y,Ogawa M

    更新日期:1988-08-01 00:00:00

  • Intensive management of high-utilizing adults with sickle cell disease lowers admissions.

    abstract::A minority of super-utilizing adults with sickle cell disease (SCD) account for a disproportionate number of emergency department (ED) and hospital admissions. We performed a retrospective cohort study comparing the rate of admission before and after the opening of a clinic for adults with SCD. Unique to this clinic w...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23912

    authors: Koch KL,Karafin MS,Simpson P,Field JJ

    更新日期:2015-03-01 00:00:00

  • Leg ulcers in sickle cell disease.

    abstract::Sickle cell disease is a single amino acid molecular disorder of hemoglobin leading to its pathological polymerization, red cell rigidity that causes poor microvascular blood flow, with consequent tissue ischemia and infarction. The manifestations of this disease are protean.Among them, leg ulcers represent a particul...

    journal_title:American journal of hematology

    pub_type:

    doi:10.1002/ajh.21838

    authors: Minniti CP,Eckman J,Sebastiani P,Steinberg MH,Ballas SK

    更新日期:2010-10-01 00:00:00

  • IDH mutations are closely associated with mutations of DNMT3A, ASXL1 and SRSF2 in patients with myelodysplastic syndromes and are stable during disease evolution.

    abstract::Current information about clinical significance of IDH mutations in myelodysplastic syndromes (MDS), their association with other genetic alterations and the stability during disease progression is limited. In this study, IDH mutations were identified in 4.6% of 477 patients with MDS based on the FAB classification an...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23596

    authors: Lin CC,Hou HA,Chou WC,Kuo YY,Liu CY,Chen CY,Lai YJ,Tseng MH,Huang CF,Chiang YC,Lee FY,Liu MC,Liu CW,Tang JL,Yao M,Huang SY,Ko BS,Wu SJ,Tsay W,Chen YC,Tien HF

    更新日期:2014-02-01 00:00:00

  • Ultrasound-guided fine needle biopsy of the spleen: high clinical efficacy and low risk in a multicenter Italian study.

    abstract::The aim of this study was to evaluate the clinical efficacy and safety of the ultrasound-guided fine needle biopsy (UG-FNB) of the spleen in a large population of patients. We collected retrospectively the findings concerning the application of UG-FNB of the spleen from eight Italian clinical centers that utilized thi...

    journal_title:American journal of hematology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ajh.1085

    authors: Civardi G,Vallisa D,Bertè R,Giorgio A,Filice C,Caremani M,Caturelli E,Pompili M,De Sio I,Buscarini E,Cavanna L

    更新日期:2001-06-01 00:00:00

  • Sickle red cell calcium metabolism: studies on Ca2+-Mg2+ATPase and Ca-binding properties of sickle red cell membranes.

    abstract::Sickle (Hb SS) red cells, preloaded with 45Ca by reversal of hemolysis, exhibit an incomplete 45Ca extrusion, retaining approximately four times more 45Ca than normal cells. Studies indicated that neither the reduction in Hb SS cell Ca2+-Mg2+ ATPase activity (84% of normal) nor the activation of Ca2+-Mg2+ ATPase by ca...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830080406

    authors: Litosch I,Lee KS

    更新日期:1980-01-01 00:00:00

  • Prognostic factors in patients with HIV-associated peripheral T-cell lymphoma: a multicenter study.

    abstract::HIV infection has been associated with an increased risk of developing several types of malignancies, including aggressive peripheral T-cell lymphomas (PTCL). However, this is a rare occurrence with no more than a hundred cases reported in the literature. The purpose of this multicenter study is to describe the charac...

    journal_title:American journal of hematology

    pub_type: 杂志文章,多中心研究

    doi:10.1002/ajh.21947

    authors: Castillo JJ,Beltran BE,Bibas M,Bower M,Collins JA,Cwynarski K,Diez-Martin JL,Hernandez-Ilizaliturri F,Horwitz SM,Montoto S,Pantanowitz L,Ribera JM,Vose JM

    更新日期:2011-03-01 00:00:00

  • Management of cancer-associated thrombotic microangiopathy: what is the right approach?

    abstract::A 49-year-old Caucasian woman presented with features suggestive of thrombotic microangiopathy (TMA). She did not respond to treatment with repeated plasma exchange and corticosteroids. A bone marrow biopsy revealed presence of metastatic carcinoma. A limited autopsy revealed presence of breast cancer with rib metasta...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.20783

    authors: Werner TL,Agarwal N,Carney HM,Rodgers GM

    更新日期:2007-04-01 00:00:00

  • B-cell chronic lymphocytic leukaemia: prognostic value of the immunophenotype and the clinico-haematological features.

    abstract::Sixty-two previously untreated patients with B-cell chronic lymphocytic leukaemia were analysed to study the prognostic value of both the immunologic phenotype and the clinicobiologic characteristics. Univariate studies showed that none of the immunological markers analysed, sheep-rosette, mouse-rosette, slg, and HLA/...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830310105

    authors: Orfao A,Gonzalez M,San Miguel JF,Rios A,Canizo MC,Hernandez J,Maricato ML,Lopez Borrasca A

    更新日期:1989-05-01 00:00:00

  • Community experience with bortezomib in patients with multiple myeloma.

    abstract::Community practice experience allows a nonselective care of patient using information derived from a more controlled clinical trial environment. We present our community experience with multiple myeloma patients with advanced age, long disease duration since diagnosis, advanced stage, multiple prior therapies includin...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.20883

    authors: Onitilo AA,Engel J,Olatosi B,Fagbemi S

    更新日期:2007-07-01 00:00:00

  • High Ia (HLA-DR) and low CD11b (Mo1) expression may predict early conversion to leukemia in myelodysplastic syndromes.

    abstract::The FAB classification of myelodysplastic syndromes (MDS) has been useful in predicting prognosis; however, additional methods are required to detect patients at high risk for early conversion to acute nonlymphoblastic leukemia (ANLL). Using a panel of monoclonal antibodies to myelomonocytic surface antigens (MMSA) an...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.2830430302

    authors: Mittelman M,Karcher DS,Kammerman LA,Lessin LS

    更新日期:1993-07-01 00:00:00

  • Prognostic value of bone marrow angiogenesis in multiple myeloma: use of light microscopy as well as computerized image analyzer in the assessment of microvessel density and total vascular area in multiple myeloma and its correlation with various clinical

    abstract::We studied the prognostic value of parameters of angiogenesis on bone marrow biopsies in newly diagnosed multiple myeloma (MM) patients. Angiogenesis parameters studied were the microvessel count done manually on light microscopy (MVD-A), microvessel count done by using computerized image analyzer (MVD-B), and total v...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.20639

    authors: Bhatti SS,Kumar L,Dinda AK,Dawar R

    更新日期:2006-09-01 00:00:00

  • Geographic variability of hemophilia-associated AIDS in the United States: effect of population characteristics. Hemophilia-Associated AIDS Study Group.

    abstract::National AIDS surveillance suggests that approximately 3.5% of all hemophilic patients in the United States have developed AIDS as of February 1988; however, the cumulative incidence of AIDS among seropositive patients at individual hemophilia treatment centers (HTCs) has been reported to be as high as 12%. The purpos...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830320304

    authors: Stehr-Green JK,Jason JM,Evatt BL

    更新日期:1989-11-01 00:00:00

  • On the trail of blood doping-microRNA fingerprints to monitor autologous blood transfusions in vivo.

    abstract::Autologous blood doping refers to the illegal re-transfusion of any quantities of blood or blood components with blood donor and recipient being the same person. The re-transfusion of stored erythrocyte concentrates is particularly attractive to high-performance athletes as this practice improves their oxygen capacity...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.26078

    authors: Mussack V,Wittmann G,Pfaffl MW

    更新日期:2020-12-16 00:00:00

  • Endothelial cells injured by oxidized low density lipoprotein.

    abstract::Cultured endothelial cells from bovine aorta were exposed to oxidized low density lipoprotein and examined by electron microscopy. The endothelial cells contracted slightly and the intercellular junctions became unclear. Some osmiophilic material increased in the cytoplasm. The oxidized low density lipoprotein appears...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830490315

    authors: Zhao B,Zhang Y,Liu B,Nawroth P,Dierichs R

    更新日期:1995-07-01 00:00:00

  • Increased numbers of marrow basophils may be associated with a t(6;9) in ANLL.

    abstract::We have characterized another subset of acute nonlymphocytic leukemia (ANLL) based on the cytogenetic and morphologic findings in a group of nine patients. Five patients had chromosomal analyses performed at the University of Chicago, two patients were studied at the All-Union Cancer Research Center in Moscow, and one...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.2830180409

    authors: Pearson MG,Vardiman JW,Le Beau MM,Rowley JD,Schwartz S,Kerman SL,Cohen MM,Fleischman EW,Prigogina EL

    更新日期:1985-04-01 00:00:00

  • Genetic polymorphisms in oxidative stress-related genes are associated with outcomes following treatment for aggressive B-cell non-Hodgkin lymphoma.

    abstract::Variable survival outcomes are seen following treatment for aggressive non-Hodgkin lymphoma (NHL). This study examined whether outcomes for aggressive B-cell NHL are associated with single nucleotide polymorphisms (SNPs) in oxidative stress-related genes, which can alter drug metabolism and immune responses. Genotypes...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23709

    authors: Gustafson HL,Yao S,Goldman BH,Lee K,Spier CM,LeBlanc ML,Rimsza LM,Cerhan JR,Habermann TM,Link BK,Maurer MJ,Slager SL,Persky DO,Miller TP,Fisher RI,Ambrosone CB,Briehl MM

    更新日期:2014-06-01 00:00:00

  • Lenalidomide in multiple myeloma: Current status and future potential.

    abstract::The clinical development of lenalidomide (Revlimid™), then pomalidomide (Actimid™) as members of immunomodulatory drugs (IMiDs) for the treatment of multiple myeloma (MM), exemplifies how insight into disease biology can lead to design of effective therapeutic agents. Increased experience and understanding of IMiD's d...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.23234

    authors: Quach H,Kalff A,Spencer A

    更新日期:2012-12-01 00:00:00

  • The "Hageman" connection: interrelationships of blood coagulation, fibrino(geno)lysis, kinin generation, and complement activation.

    abstract::The activation pathways for the generation of enzymes involved in blood clotting, clot lysis, complement activation, and kinin generation are briefly reviewed. The interrelationship of the four systems is illustrated by the multiple functions of four key enzymes: Factor XIIa, kallikrein, plasmin, and C1 esterase. The ...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审

    doi:10.1002/ajh.2830040412

    authors: Murano G

    更新日期:1978-01-01 00:00:00

  • Chronic granulomatous disease: Clinical, functional, molecular, and genetic studies. The Israeli experience with 84 patients.

    abstract::Chronic granulomatous disease (CGD) is an innate immunodeficiency with a genetic defect of the nicotinamide adenosine dinucleotide phosphate, reduced, oxidase components. This leads to decreased reactive oxygen species (ROS) production, which renders patients susceptible to life-threatening infections. Over the course...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.24573

    authors: Wolach B,Gavrieli R,de Boer M,van Leeuwen K,Berger-Achituv S,Stauber T,Ben Ari J,Rottem M,Schlesinger Y,Grisaru-Soen G,Abuzaitoun O,Marcus N,Zion Garty B,Broides A,Levy J,Stepansky P,Etzioni A,Somech R,Roos D

    更新日期:2017-01-01 00:00:00

  • Altered cytokine profiles in patients with Chuvash polycythemia.

    abstract::Chuvash polycythemia results from a homozygous 598C>T mutation in exon 3 of the von Hippel-Lindau (VHL) gene. This disrupts the normoxia pathway for degrading hypoxia inducible factor (HIF)-1alpha and HIF-2alpha causing altered expression of HIF-1 and HIF-2 inducible genes. As hypoxia induces expression of pro-inflamm...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.21327

    authors: Niu X,Miasnikova GY,Sergueeva AI,Polyakova LA,Okhotin DJ,Tuktanov NV,Nouraie M,Ammosova T,Nekhai S,Gordeuk VR

    更新日期:2009-02-01 00:00:00

  • Does iron overload really matter in stem cell transplantation?

    abstract::A growing body of evidence suggests that iron overload is associated with inferior outcomes after myeloablative allogeneic hematopoietic stem cell transplantation (HSCT). However, all of those studies used surrogate markers of iron overload, especially serum ferritin, and most had a retrospective design. We conducted ...

    journal_title:American journal of hematology

    pub_type: 杂志文章

    doi:10.1002/ajh.23188

    authors: Armand P,Sainvil MM,Kim HT,Rhodes J,Cutler C,Ho VT,Koreth J,Alyea EP,Neufeld EJ,Kwong RY,Soiffer RJ,Antin JH

    更新日期:2012-06-01 00:00:00