Abstract:
:We describe the synthesis of 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2'-O-modified compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2'-O-methyl and 2'-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1-10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cotten M,Oberhauser B,Brunar H,Holzner A,Issakides G,Noe CR,Schaffner G,Wagner E,Birnstiel MLdoi
10.1093/nar/19.10.2629subject
Has Abstractpub_date
1991-05-25 00:00:00pages
2629-35issue
10eissn
0305-1048issn
1362-4962journal_volume
19pub_type
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