A gene delivery approach for antimicrobials: expression of defensins.

Abstract:

INTRODUCTION:Peptide antibiotics as new therapeutic agents are becoming a popular option to investigate due to their broad bacterial target selectivity and limited resistance problems. Although attractive, these new drug candidates have several limitations including low potency and delivery issues which face all peptides/proteins. METHODS:In this study, we designed a plasmid expression system for human beta defensin 3. This sequence was cloned from a human epithelial lung cell into a CMV driven expression cassette. This expression plasmid was then evaluated for its ability to produce human-beta defensin 3 with the use of the non-viral transfection agent, polyethylenimine (PEI). RESULTS:The results indicate the expression cassette was transcriptionally active in HEK 293 cells, as measured by RT-PCR and that a beta defensin peptide was produced by the cells as confirmed by Western blot. The biological activity of the peptide was confirmed against both gram negative E. coli and gram positive Bacillus species using in vitro screening. CONCLUSION:Both the cultured media as well as the transfected cell lysate demonstrated biological activity demonstrating the peptide is also secreted.

journal_name

J Drug Target

authors

Zhang C,Yadava P,Sun J,Hughes JA

doi

10.1080/10611860600907767

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

646-51

issue

9

eissn

1061-186X

issn

1029-2330

pii

UQU28G08315T1816

journal_volume

14

pub_type

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