Abstract：
:The kinetics of the association and dissociation of the tritium-labeled selective and potent dopamine transporter inhibitor N-(3-iodoprop-2E-enyl)-2beta-carbo-[3H]methoxy-3beta-(4'-methylphenyl)nortropane ([3H]PE2I) with the transporter of mouse striatal membranes was studied. The analysis revealed that the specific binding of [3H]PE2I occurs within a homogeneous population of binding sites in these membranes. The relatively slow binding process was characterized by the pseudo-first-order rate constant kobs. The plot of these rate constants versus free radioligand concentration was hyperbolic, demonstrating that at least two kinetically distinguishable steps can be identified in the interaction of dopamine transporter with this inhibitor. The fast and reversible binding step, characterized by dissociation constant KA = 51 +/- 23 nM, is followed by a slow but also reversible isomerization step of the complex, characterized by the isomerization rate constant ki = (7 +/- 2)10(-2) s(-1) and by the rate constant k(-i) = (3.9 +/- 0.5)10(-3) s(-1) for the reverse process. This isomerization step increases the apparent affinity of the ligand and probably consists of a conformational transition of the transporter protein, induced by the inhibitor molecule.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Stepanov V,Järv Jdoi
10.1016/j.neulet.2006.10.007subject
Has Abstractpub_date
2006-12-27 00:00:00pages
218-21issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(06)01057-3journal_volume
410pub_type
杂志文章abstract：:Preterm infants undergo several painful procedures during their stay in neonatal intensive care units. Previous studies suggest that early painful experiences may have an impact on brain development. Here, we used an animal model to investigate the effect of neonatal pain on the generation of new neurons in the dentat...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.06.047
更新日期：2011-08-26 00:00:00
abstract：:Single-site variants in the calmodulin-binding domain of RC3/neurogranin were heterologously expressed in Xenopus oocytes to examine their effects on serotonin-evoked currents. RC3 variants serine36 -->alanine (Ser36-->Ala), serine36-->glycine (Ser36-->Gly), and phenylalanine37-->tryptophan (Phe37-->Trp), which bind c...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(96)13203-1
更新日期：1996-11-29 00:00:00
abstract：:In the present study, we demonstrate that the histopathologic features of myelin oligodendrocyte glycoprotein (MOG) peptide 35-55-induced experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice closely mirror the hallmarks of MS pathology. On the one hand, we depict a time-dependent transition from acute infla...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.03.021
更新日期：2011-05-02 00:00:00
abstract：:Brain derived neurotrophic factor (BDNF), known to promote survival of neurons, has recently been involved in the regulation of endothelial cells and was shown to possess immunoregulatory properties. We therefore asked whether BDNF has immunoregulating effects in human cerebral endothelial cells (HCEC). Adult HCEC exp...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00778-4
更新日期：2002-09-20 00:00:00
abstract：:The present study investigates the effect of cognitive reappraisal on emotion regulation in socially anxious individuals. Twenty-eight female subjects were divided into high-socially anxious (HSA) and low-socially anxious (LSA) groups. All subjects viewed threatening faces under cognitive reappraisal and passive viewi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.06.006
更新日期：2014-08-08 00:00:00
abstract：:Outcome following traumatic brain injury (TBI) is in large part determined by the combined action of multiple processes. In order to better understand the response of the central nervous system to injury, we utilized an antibody array to simultaneously screen 507 proteins for altered expression in the injured hippocam...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.11.060
更新日期：2007-02-08 00:00:00
abstract：:The opioid peptide dynorphin A(1-8) (1 micron) increased acetylcholine release from the Torpedo electric organ by approximately twofold. This effect was reversed by the opiate antagonist naloxone. The effect of Dyn A(1-8) on acetylcholine release was found to vary in magnitude with the seasons of the year, with maxima...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90036-s
更新日期：1991-04-29 00:00:00
abstract：:Convulsions were induced in rats by a single injection of pentylenetetrazol (PTZ) and [3H]L-2-amino-4-phosphonobutyrate ([3H]L-AP4) in vitro receptor autoradiography was used to evaluate the effects on the expression of group III metabotropic glutamate receptors. Significant increases by 60-80% in [3H]L-AP4 binding wa...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00235-9
更新日期：1999-04-30 00:00:00
abstract：:Nogo-A has been identified as an inhibitory molecule to neurite outgrowth after injury in adult mammalian central nervous system (CNS). The C-terminal fragment of Nogo-A, Nogo-66, inhibits axonal regrowth through NgR1 signaling. Residues 1-32 of Nogo-66 cover two regions that contribute most affinity of Nogo-66 to NgR...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.01.009
更新日期：2013-03-01 00:00:00
abstract：:The localization of serotonin and non-serotonin-containing cell bodies in the interpeduncular nucleus of the rat that project to the hippocampal formation was studied using the technique of retrograde tracing of Granular Blue and immunohistochemistry on the same sections. The results indicate that the caudal magnocell...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(84)90256-8
更新日期：1984-09-28 00:00:00
abstract：:It is well-established that 17beta-estradiol (17beta-E(2)) confers neuroprotection to male and female rats exposed to focal cerebral ischemia, while less is known about the effects of the hormone under conditions of transient global ischemia. Since translocation of cytochrome c from the mitochondria to the cytosol is ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.06.062
更新日期：2004-09-16 00:00:00
abstract：:Lucifer Yellow (LY) selectively labels embryonic chick amacrine cells from days 11 until 14 in vivo. Its usefulness as an in vitro marker was investigated. In vivo labeling and subsequent culturing was not possible due to dye leakage. Neurons, however, could be labeled at various times in vitro. The number of neurons ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(86)90341-1
更新日期：1986-03-14 00:00:00
abstract：:During hyperalgesia there is an enhancement of wind-up and the appearance of a novel wind-up of the A-fibre-mediated responses. We have examined if these phenomena are influenced by supraspinal mechanisms by analysing single motor unit activity in control and arthritic rats, either intact or acutely spinalised. Enhanc...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12588-x
更新日期：1996-05-03 00:00:00
abstract：:In this study we evaluated oxidative stress (lipid peroxidation and protein oxidation) and thiol redox state [TRS: glutathione (GSH), glutathione disulfide (GSSG), cysteine (CSH), protein (P) thiols (PSH) and protein and non-protein (NP) mixed/symmetric disulfides (PSSR, NPSSR, NPSSC, PSSP)] in hippocampus after penty...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.10.080
更新日期：2004-03-04 00:00:00
abstract：:Occurring at rates up to 6-7 syllables per second, speech perception and understanding involves rapid identification of speech sounds and pre-activation of morphemes and words. Using event-related potentials (ERPs) and functional magnetic resonance imaging (fMRI), we investigated the time-course and neural sources of ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.08.030
更新日期：2017-09-29 00:00:00
abstract：:The effect of pineal indoles on fast axoplasmic transport of proteins in the sciatic nerve was examined in rats injected with [3H]leucine in the sixth lumbar dorsal root ganglion. Melatonin (350-1100 nmol) applied locally in the sciatic nerve impaired significantly axonal transport. At a 350-nmol dose all other indole...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(79)90070-3
更新日期：1979-06-01 00:00:00
abstract：:A study was carried out to determine at what stage of development the adult pattern of primary afferent projections to the substantia gelatinosa (SG) is first established. Horseradish peroxidase conjugated wheat-germ agglutinin (WGA-HRP) was applied to cut ends of sciatic nerves at mid-thigh level in adult and neonata...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(83)90179-9
更新日期：1983-12-30 00:00:00
abstract：:'Reverspectives' (by artist Patrick Hughes) consist of truncated pyramids with their small faces closer to the viewer, allowing realistic scenes to be painted on them. Because their pictorial perspective reverses the physical depth arrangement, reverspectives provide a bistable paradigm of two radically different, com...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.10.093
更新日期：2009-01-09 00:00:00
abstract：:The objective of the present study was to characterize the trkB receptor immunoreactive (-ir) cells in the intermediolateral cell column (IML) of the upper thoracic spinal cord. Small trkB-ir cells (area=56.1+/-4.4 microm(2)) observed in the IML showed characteristics of oligodendrocytes and were frequently observed i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.05.057
更新日期：2008-08-01 00:00:00
abstract：:Nociceptin/orphanin FQ (NC) and its receptor (OP(4)) have been implicated in the regulation of various functions including nociception. [Nphe(1)]NC(1-13)NH(2) (Nphe) is a selective OP(4) antagonist which prevents the pronociceptive effects of supraspinal NC and causes per se a naloxone-insensitive antinociceptive effe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02352-7
更新日期：2001-12-04 00:00:00
abstract：:Parkinson's disease (PD), caused by the decreased number of dopaminergic neurons in the substantia nigra, is identified as the second most familiar age-dependent neurodegenerative disease to the public. Long non-coding RNAs (lncRNAs) have been reported to participate in the development of PD. In our research, the expr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135602
更新日期：2021-01-06 00:00:00
abstract：:The magnocellular neurones of the supraoptic nucleus (SON) and paraventricular nucleus release neuropeptide from their axon terminals and also from their dendrites. In the axon terminals, swellings known as Herring bodies are responsible for the degradation of aged, unreleased large dense-cored vesicles (LDCVs) by lys...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.11.060
更新日期：2011-02-01 00:00:00
abstract：:The content of the neurosteroids, dehydroepiandrosterone (DHEA) in the brain decreases with aging. Also the oxidative energy metabolism is known to decrease with aging. Hence we examined the effects of treatment with DHEA (0.2 or 1.0 mg/kg body weight for 7 days) on oxidative energy metabolism in brain mitochondria fr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.03.057
更新日期：2006-07-10 00:00:00
abstract：:In the superoxide dismutase 1 (SOD1)(G93A G1H) transgenic mouse, the primary pathology and disease signs are associated with the degeneration of motor neurons in the lumbar spinal cord. It is unclear if the descending motor pathways from the cortex and brainstem are also compromised. The retrograde tracer Fluorogold w...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00944-8
更新日期：2002-10-31 00:00:00
abstract：:Approximately 30% of all epilepsy cases are acquired. At present there is no effective strategy to stop epilepsy development after the precipitating insult. Recent data from experimental models pointed to the mTOR pathway, which can be potently inhibited by rapamycin. However, data on the antiepileptic and antiepilept...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.12.051
更新日期：2012-02-16 00:00:00
abstract：:Reactive oxygen species and inflammatory signaling have been identified as pivotal pathophysiological factors contributing to epileptogenesis. Considering the development of combined anti-inflammatory and antioxidant treatment strategies with antiepileptogenic potential, a characterization of the time course of microg...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.05.041
更新日期：2015-07-10 00:00:00
abstract：:Motor and sensory neuropathies with the clinical features of HMSN III (Dejerine-Sottas syndrome, DSS) are etiologically related to heterozygous mutations in either peripheral myelin protein-22 (PMP22) or myelin protein zero (MPZ). Heterozygous mutations in either of these two genes are also responsible for other hered...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)00887-2
更新日期：1998-01-02 00:00:00
abstract：:Effects of substance P and extracellular [K(+)](o) on respiratory motor activity in the ventrolateral medulla in neonatal rat (0-4 days old) brainstem-spinal cord preparation were studied. In addition to fictive eupneic rhythm (8-13 bursts/minute), the respiratory motor output was composed of biphasic bursts which mig...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00645-1
更新日期：2003-08-14 00:00:00
abstract：:Cerebral perfusion is important in older adults as it is linked to cognitive declines. Physical activity can improve blood flow in the body but little is known about the relationship between physical activity and cerebral perfusion in older adults. In particular, no study has investigated the relation between strength...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.12.011
更新日期：2014-02-07 00:00:00
abstract：:Inattention and impulsivity are the two most important indices for evaluations of ADHD. Currently, inattention and impulsivity were evaluated by clinical scales. The intelligent evaluation of the two indices using machine learning remains largely unexplored. This paper aimed to build regression modes for inattention a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.134874
更新日期：2020-04-17 00:00:00
