In vivo confocal fluorescence imaging of the intratumor distribution of the photosensitizer mono-L-aspartylchlorin-e6.

Abstract:

:We present an in vivo fluorescence microscopic evaluation of intratumor distribution of the photosensitizer mono-L-aspartylchlorin-e6 (NPe6) in an intradermal mouse EMT6 tumor model. Although the identification of favorable photophysical and pharmacological properties has led to the development of new photosensitizers in photodynamic therapy, their intratumor distribution kinetics have remained relatively understudied. In this study, we used confocal fluorescence microscopy to follow the transport of NPe6 in vivo after systemic administration through the tail vein. Labeling of vasculature using fluorophore-conjugated anti-CD31 antibodies allows visualization of the uptake of NPe6 in tumor and normal vessels and its partitioning kinetics into the adjacent parenchyma for 3 hours after injection. During the initial 60 minutes after injection, the drug is predominantly confined to the vasculature. Subsequently, it significantly redistributes throughout the extravascular regions with no discernable difference in its extravasation rate between tumor and normal tissues. Further, we investigate the sensitizer's altered intratumor distribution in response to photodynamic therapy irradiation and observe that treatment-induced changes in vessel permeability caused enhanced accumulation of NPe6 in the extravascular space. Our findings are of immediate clinical relevance and demonstrate the importance of an in vivo imaging approach to examine the dynamic process of intratumor drug distribution.

journal_name

Neoplasia

authors

Mitra S,Foster TH

doi

10.1593/neo.08104

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

429-38

issue

5

eissn

1522-8002

issn

1476-5586

journal_volume

10

pub_type

杂志文章
  • Fibrin Deposit on the Peritoneal Surface Serves as a Niche for Cancer Expansion in Carcinomatosis Patients.

    abstract::Peritoneal metastasis (PM) is a very serious complication of gastrointestinal and gynecological malignancies which is poorly documented. Modified mesothelial cell layer and their microenvironments can favor fibrin deposition for cancer cell adhesion. Scanning and transmission electron microscopy of peritoneal surface ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1016/j.neo.2019.08.006

    authors: Shahid S,Iman A,Matti U,Rachid K,Assaf A,Eveno C,Marc P,Massoud M

    更新日期:2019-11-01 00:00:00

  • Epstein-Barr virus infection alters cellular signal cascades in human nasopharyngeal epithelial cells.

    abstract::Epstein-Barr virus (EBV) latent infection is a critical event in nasopharyngeal carcinoma (NPC) tumorigenesis. EBV-encoded genes have been shown to be involved in immune evasion and in the regulation of various cellular signaling cascades. To elucidate the roles of EBV in NPC development, stable infection of EBV in na...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.05625

    authors: Lo AK,Lo KW,Tsao SW,Wong HL,Hui JW,To KF,Hayward DS,Chui YL,Lau YL,Takada K,Huang DP

    更新日期:2006-03-01 00:00:00

  • An alternate splicing variant of the human telomerase catalytic subunit inhibits telomerase activity.

    abstract::Telomerase, a cellular reverse transcriptase, adds telomeric repeats to chromosome ends. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to proliferative senescence. Introduction of the telomerase (hTERT) cDNA is sufficient to produce telomerase activity and immortal...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1038/sj.neo.7900113

    authors: Yi X,White DM,Aisner DL,Baur JA,Wright WE,Shay JW

    更新日期:2000-09-01 00:00:00

  • Distinct patterns of structural and numerical chromosomal instability characterize sporadic ovarian cancer.

    abstract::Sporadic ovarian cancer is a particularly aggressive tumor characterized by highly abnormal karyotypes exhibiting many features of genomic instability. More complex genomic changes in tumors arise as a consequence of chromosomal instability (CIN), which can generate both numerical [(N)-CIN] and structural chromosomal ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.08584

    authors: Bayani J,Paderova J,Murphy J,Rosen B,Zielenska M,Squire JA

    更新日期:2008-10-01 00:00:00

  • Merlin-deficient human tumors show loss of contact inhibition and activation of Wnt/β-catenin signaling linked to the PDGFR/Src and Rac/PAK pathways.

    abstract::Neurofibromatosis type 2 (NF2) is an inherited predisposition cancer syndrome characterized by the development of multiple benign tumors in the nervous system including schwannomas, meningiomas, and ependymomas. Using a disease model comprising primary human schwannoma cells, we previously demonstrated that adherens j...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.111060

    authors: Zhou L,Ercolano E,Ammoun S,Schmid MC,Barczyk MA,Hanemann CO

    更新日期:2011-12-01 00:00:00

  • Glutamate receptor GRIA3--target of CUX1 and mediator of tumor progression in pancreatic cancer.

    abstract::Previously, we identified the transcription factor CUX1 as an important modulator of invasion and resistance to apoptosis. Expression profiles suggested that CUX1 regulates a complex transcriptional program mediating tumor progression. We aimed to identify functionally relevant targets of CUX1 by using RNA interferenc...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.10486

    authors: Ripka S,Riedel J,Neesse A,Griesmann H,Buchholz M,Ellenrieder V,Moeller F,Barth P,Gress TM,Michl P

    更新日期:2010-08-01 00:00:00

  • Telomere length on chromosome 17q shortens more than global telomere length in the development of breast cancer.

    abstract::It is known that total telomere length is shorter in invasive breast cancer than in normal breast tissue but the status of individual telomere lengths has not been studied. Part of the difficulty is that usually telomere length in interphase cells is measured on all chromosomes together. In this study we compared norm...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.07106

    authors: Rashid-Kolvear F,Pintilie M,Done SJ

    更新日期:2007-04-01 00:00:00

  • Association of the actin-binding protein transgelin with lymph node metastasis in human colorectal cancer.

    abstract::Metastatic dissemination of primary tumors is responsible for 90% of colorectal cancer (CRC) deaths. The presence of positive lymph nodes, which separates stage I/II from stage III CRC, is a particularly key factor in patient management. Here, we describe results of a quantitative proteomic survey to identify molecula...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.09542

    authors: Lin Y,Buckhaults PJ,Lee JR,Xiong H,Farrell C,Podolsky RH,Schade RR,Dynan WS

    更新日期:2009-09-01 00:00:00

  • Insulin-like growth factor-1 inscribes a gene expression profile for angiogenic factors and cancer progression in breast epithelial cells.

    abstract::Activation of the insulin-like growth factor-1 receptor (IGF-1R) by IGF-1 is associated with the risk and progression of many types of cancer, although despite this it remains unclear how activated IGF-1R contributes to cancer progression. In this study, gene expression changes elicited by IGF-1 were profiled in breas...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1038/sj.neo.7900229

    authors: Oh JS,Kucab JE,Bushel PR,Martin K,Bennett L,Collins J,DiAugustine RP,Barrett JC,Afshari CA,Dunn SE

    更新日期:2002-05-01 00:00:00

  • Deregulated expression of the human tumor marker CEA and CEA family member CEACAM6 disrupts tissue architecture and blocks colonocyte differentiation.

    abstract::Human carcinoembryonic antigen (CEA) and the CEA family member CEACAM6 (formerly nonspecific cross-reacting antigen [NCA]) function in vitro, at least, as homotypic intercellular adhesion molecules and, in model systems, can block the terminal differentiation and anoikis of several different cell types. We have recent...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1038/sj.neo.7900201

    authors: Ilantzis C,DeMarte L,Screaton RA,Stanners CP

    更新日期:2002-03-01 00:00:00

  • Clinical implications of promoter hypermethylation in RASSF1A and MGMT in retinoblastoma.

    abstract::We investigated the epigenetic silencing and genetic changes of the RAS-associated domain family 1A (RASSF1A) gene and the O6-methylguanine-DNA methyltransferase (MGMT) gene in retinoblastoma. We extracted DNA from microdissected tumor and normal retina tissues of the same patient in 68 retinoblastoma cases. Promoter ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.04565

    authors: Choy KW,Lee TC,Cheung KF,Fan DS,Lo KW,Beaverson KL,Abramson DH,Lam DS,Yu CB,Pang CP

    更新日期:2005-03-01 00:00:00

  • CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling.

    abstract::Gastrointestinal stromal tumors (GISTs) are the major gastrointestinal mesenchymal tumors with a variable malignancy ranging from a curable disorder to highly malignant sarcomas. Metastasis and recurrence are the main causes of death in GIST patients. To further explore the mechanism of metastasis and to more accurate...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1016/j.neo.2014.03.001

    authors: Ma MZ,Zhuang C,Yang XM,Zhang ZZ,Ma H,Zhang WM,You H,Qin W,Gu J,Yang S,Cao H,Zhang ZG

    更新日期:2014-03-01 00:00:00

  • The acetyltransferase p300/CBP-associated factor is a p53 target gene in breast tumor cells.

    abstract::p300/CBP-associated factor (PCAF) is a coactivator of the tumor suppressor, p53. PCAF participates in p53's transactivation of target genes through acetylation of both bound p53 and histones within p53 target promoters. Using microarrays, we discovered that PCAF itself is induced by p53 in a panel of breast tumor cell...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.3292

    authors: Watts GS,Oshiro MM,Junk DJ,Wozniak RJ,Watterson S,Domann FE,Futscher BW

    更新日期:2004-05-01 00:00:00

  • Alternative splicing of CHEK2 and codeletion with NF2 promote chromosomal instability in meningioma.

    abstract::Mutations of the NF2 gene on chromosome 22q are thought to initiate tumorigenesis in nearly 50% of meningiomas, and 22q deletion is the earliest and most frequent large-scale chromosomal abnormality observed in these tumors. In aggressive meningiomas, 22q deletions are generally accompanied by the presence of large-sc...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.111574

    authors: Yang HW,Kim TM,Song SS,Shrinath N,Park R,Kalamarides M,Park PJ,Black PM,Carroll RS,Johnson MD

    更新日期:2012-01-01 00:00:00

  • Noninvasive monitoring of breast cancer during neoadjuvant chemotherapy using optical tomography with ultrasound localization.

    abstract::The purposes of this study were 1) to investigate the feasibility of using optical tomography in the near-infrared (NIR) spectrum combined with ultrasound (US) localization (NIR/US) in monitoring tumor vascular changes and assessing tumor pathological response during chemotherapy and 2) to compare the accuracy of NIR/...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 临床试验,杂志文章

    doi:10.1593/neo.08602

    authors: Zhu Q,Tannenbaum S,Hegde P,Kane M,Xu C,Kurtzman SH

    更新日期:2008-10-01 00:00:00

  • Tracking the migration of dendritic cells by in vivo optical imaging.

    abstract::We report herein a method to track the migration of dendritic cells (DCs) using optical imaging. With the assistance of the delivery module, fluorescein isothiocyanate (FITC) could internalize inside DCs within 15 minutes of incubation. The fluorescent signal was mostly cytoplasmic and could be detected using in vivo ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.07586

    authors: Pham W,Xie J,Gore JC

    更新日期:2007-12-01 00:00:00

  • Two-mechanism peak concentration model for cellular pharmacodynamics of Doxorubicin.

    abstract::A mathematical model is presented for the cellular uptake and cytotoxicity of the anticancer drug doxorubicin. The model assumes sigmoidal, Hill-type dependence of cell survival on drug-induced damage. Experimental evidence indicates distinct intracellular and extracellular mechanisms of doxorubicin cytotoxicity. Drug...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.05118

    authors: El-Kareh AW,Secomb TW

    更新日期:2005-07-01 00:00:00

  • Bcl-2 and M-Myc coexpression increases IGF-IR and features of malignant growth in neuroblastoma cell lines.

    abstract::The bcl-2 and c-myc oncogenes cooperate to transform multiple cell types. In the pediatric malignancy NB(2), Bcl-2 is highly expressed. In tumors with a poor prognosis, N-Myc, a protein homologous to c-Myc, is overexpressed as a result of gene amplification. The present study was designed to determine whether Bcl-2 co...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1038/sj.neo.7900171

    authors: Jasty R,van Golen C,Lin HJ,Solomon G,Heidelberger K,Polverini P,Opipari A,Feldman E,Castle VP

    更新日期:2001-07-01 00:00:00

  • Changes in water mobility measured by diffusion MRI predict response of metastatic breast cancer to chemotherapy.

    abstract::The goal of oncology is the individualization of patient care to optimize therapeutic responses and minimize toxicities. Achieving this will require noninvasive, quantifiable, and early markers of tumor response. Preclinical data from xenografted tumors using a variety of antitumor therapies have shown that magnetic r...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.03343

    authors: Theilmann RJ,Borders R,Trouard TP,Xia G,Outwater E,Ranger-Moore J,Gillies RJ,Stopeck A

    更新日期:2004-11-01 00:00:00

  • Synergism of BARF1 with Ras induces malignant transformation in primary primate epithelial cells and human nasopharyngeal epithelial cells.

    abstract::Although it is well known that nasopharyngeal carcinoma (NPC) is closely related with Epstein-Barr virus (EBV), few data are available about which and how EBV-expressed gene is involved in the carcinogenesis of human nasopharyngeal epithelial cells. EBV-encoded BARF1 (BamH I-A right frame 1) gene has been shown to be ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.09706

    authors: Jiang R,Cabras G,Sheng W,Zeng Y,Ooka T

    更新日期:2009-09-01 00:00:00

  • Isolation of a novel human gene, MARKL1, homologous to MARK3 and its involvement in hepatocellular carcinogenesis.

    abstract::Activation of the Wnt-signaling pathway is known to play a crucial role in carcinogenesis of various human organs including the colon, liver, prostate, and endometrium. To investigate the mechanisms underlying hepatocellular carcinogenesis, we attempted to identify genes regulated by beta-catenin/Tcf complex in a huma...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1038/sj.neo.7900132

    authors: Kato T,Satoh S,Okabe H,Kitahara O,Ono K,Kihara C,Tanaka T,Tsunoda T,Yamaoka Y,Nakamura Y,Furukawa Y

    更新日期:2001-01-01 00:00:00

  • Valproic acid upregulates NKG2D ligand expression through an ERK-dependent mechanism and potentially enhances NK cell-mediated lysis of myeloma.

    abstract::Modulation of the antitumor immune response through the engagement of NKG2D receptors with their ligands (L) on targets represents a promising therapeutic approach against cancer. In this study, we tested the effect of valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, on the expression of NKG2D ligands in m...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.121236

    authors: Wu X,Tao Y,Hou J,Meng X,Shi J

    更新日期:2012-12-01 00:00:00

  • 15-lipoxygenase-1 production is lost in pancreatic cancer and overexpression of the gene inhibits tumor cell growth.

    abstract::Pancreatic cancer patients have an abysmal prognosis because of late diagnosis and lack of therapeutic options. Pancreatic intraepithelial neoplasias (PanINs), the precursor lesions, are a potential target for chemoprevention. Targeting eicosanoid pathways is an obvious choice because 5-lipoxygenase (5-LOX) has been s...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.07565

    authors: Hennig R,Kehl T,Noor S,Ding XZ,Rao SM,Bergmann F,Fürstenberger G,Büchler MW,Friess H,Krieg P,Adrian TE

    更新日期:2007-11-01 00:00:00

  • Improved clearance during treatment of HPV-positive head and neck cancer through mTOR inhibition.

    abstract::Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) incidence is increasing at a near epidemic rate. We investigated whether the mammalian (or mechanistic) target of rapamycin (mTOR) inhibitor, rapamycin, can be used as a concurrent agent to standard-of-care cisplatin/radiation therapy (CR...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.13432

    authors: Coppock JD,Wieking BG,Molinolo AA,Gutkind JS,Miskimins WK,Lee JH

    更新日期:2013-06-01 00:00:00

  • Impact of metronomic UFT/cyclophosphamide chemotherapy and antiangiogenic drug assessed in a new preclinical model of locally advanced orthotopic hepatocellular carcinoma.

    abstract::Hepatocellular carcinoma (HCC) is an intrinsically chemotherapy refractory malignancy. Development of effective therapeutic regimens would be facilitated by improved preclinical HCC models. Currently, most models consist of subcutaneous human tumor transplants in immunodeficient mice; however, these do not reproduce t...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.91872

    authors: Tang TC,Man S,Lee CR,Xu P,Kerbel RS

    更新日期:2010-03-01 00:00:00

  • Longitudinal magnetic resonance imaging-based assessment of vascular changes and radiation response in androgen-sensitive prostate carcinoma xenografts under androgen-exposed and androgen-deprived conditions.

    abstract::Prostate cancer (PCa) patients receive androgen-deprivation therapy (ADT) to reduce tumor burden. However, complete eradication of PCa is unusual, and recurrent disease is evident within approximately 2 years in high-risk patients. Clinical evidence suggests that combining ADT with radiotherapy improves local control ...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.10484

    authors: Røe K,Seierstad T,Kristian A,Mikalsen LT,Mælandsmo GM,van der Kogel AJ,Ree AH,Olsen DR

    更新日期:2010-10-01 00:00:00

  • Cancer-associated fibroblasts and their putative role in potentiating the initiation and development of epithelial ovarian cancer.

    abstract::The progression of ovarian cancer, from cell transformation through invasion of normal tissue, relies on communication between tumor cells and their adjacent stromal microenvironment. Through a natural selection process, an autocrine-paracrine communication loop establishes reciprocal reinforcement of growth and migra...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章,评审

    doi:10.1593/neo.101720

    authors: Schauer IG,Sood AK,Mok S,Liu J

    更新日期:2011-05-01 00:00:00

  • Twist modulates breast cancer stem cells by transcriptional regulation of CD24 expression.

    abstract::The cancer stem cell paradigm postulates that dysregulated tissue-specific stem cells or progenitor cells are precursors for cancer biogenesis. Consequently, identifying cancer stem cells is crucial to our understanding of cancer progression and for the development of novel therapeutic agents. In this study, we demons...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.91084

    authors: Vesuna F,Lisok A,Kimble B,Raman V

    更新日期:2009-12-01 00:00:00

  • Synthesis, Characterization, and In Vitro and In Vivo Evaluations of 4-(N)-Docosahexaenoyl 2', 2'-Difluorodeoxycytidine with Potent and Broad-Spectrum Antitumor Activity.

    abstract::In this study, a new compound, 4-(N)-docosahexaenoyl 2', 2'-difluorodeoxycytidine (DHA-dFdC), was synthesized and characterized. Its antitumor activity was evaluated in cell culture and in mouse models of pancreatic cancer. DHA-dFdC is a poorly soluble, pale yellow waxy solid, with a molecular mass of 573.3Da and a me...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1016/j.neo.2015.11.012

    authors: Naguib YW,Lansakara-P D,Lashinger LM,Rodriguez BL,Valdes S,Niu M,Aldayel AM,Peng L,Hursting SD,Cui Z

    更新日期:2016-01-01 00:00:00

  • CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.

    abstract::Recent studies have demonstrated that the chemokine receptor CXCR4 plays a crucial role in organ-specific metastasis formation. Although a variety of studies showed the expression of chemokine receptors, in particular, CXCR4, by gastrointestinal tumors, the precise mechanisms of chemokine receptor-mediated homing of c...

    journal_title:Neoplasia (New York, N.Y.)

    pub_type: 杂志文章

    doi:10.1593/neo.09272

    authors: Gassmann P,Haier J,Schlüter K,Domikowsky B,Wendel C,Wiesner U,Kubitza R,Engers R,Schneider SW,Homey B,Müller A

    更新日期:2009-07-01 00:00:00