UV irradiation induces a postreplication DNA damage checkpoint.

abstract：:Cellular membranes are a heterogeneous mix of lipids, proteins and small molecules. Special groupings enriched in saturated lipids and cholesterol form liquid-ordered domains, known as "lipid rafts," thought to serve as platforms for signaling, trafficking and material transport throughout the secretory pathway. Quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ursell TS,Klug WS,Phillips R

更新日期：2009-08-11 00:00:00

abstract：:Tissue- and cell-specific deletion of the Aqp4 gene is required to differentiate between the numerous pools of aquaporin-4 (AQP4) water channels. A glial-conditional Aqp4 knockout mouse line was generated to resolve whether astroglial AQP4 controls water exchange across the blood-brain interface. The conditional knock...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haj-Yasein NN,Vindedal GF,Eilert-Olsen M,Gundersen GA,Skare Ø,Laake P,Klungland A,Thorén AE,Burkhardt JM,Ottersen OP,Nagelhus EA

更新日期：2011-10-25 00:00:00

abstract：:Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woloski BM,Fuller GM

更新日期：1985-03-01 00:00:00

abstract：:Previous studies have shown that DNA methyltransferase (Dnmt) 1 is required for maintenance of bulk DNA methylation and is essential for mouse development. However, somatic disruption of DNMT1 in the human cancer cell line HCT116 was not lethal and caused only minor decreases in methylation. Here, we report the identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egger G,Jeong S,Escobar SG,Cortez CC,Li TW,Saito Y,Yoo CB,Jones PA,Liang G

更新日期：2006-09-19 00:00:00

abstract：:To optimize the use of modified adenoviruses as vectors for gene delivery to the myocardium, we have characterized infection of cultured fetal and adult rat cardiac myocytes in vitro and of adult cardiac myocytes in vivo by using a replication-defective adenovirus carrying the chloramphenicol acetyltransferase (CAT) r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kass-Eisler A,Falck-Pedersen E,Alvira M,Rivera J,Buttrick PM,Wittenberg BA,Cipriani L,Leinwand LA

更新日期：1993-12-15 00:00:00

abstract：:The delta-subspecies of protein kinase C (delta PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose fast flow, phenyl 5PW, heparin 5PW, hydroxyapatite, and Mono Q columns. The purified enzyme was a doublet with molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogita K,Miyamoto S,Yamaguchi K,Koide H,Fujisawa N,Kikkawa U,Sahara S,Fukami Y,Nishizuka Y

更新日期：1992-03-01 00:00:00

abstract：:We performed the first genome-wide expression analysis directly comparing the expression profile of highly enriched normal human hematopoietic stem cells (HSC) and leukemic stem cells (LSC) from patients with acute myeloid leukemia (AML). Comparing the expression signature of normal HSC to that of LSC, we identified 3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majeti R,Becker MW,Tian Q,Lee TL,Yan X,Liu R,Chiang JH,Hood L,Clarke MF,Weissman IL

更新日期：2009-03-03 00:00:00

abstract：:An enhancer specific to erythroid cells was identified previously in the 3' flanking sequence of the chicken adult beta-globin gene and shown to act on the beta-globin promoter. This enhancer lies between the adult beta-globin gene and the embryonic epsilon-globin gene, about equidistant from the two promoters. To det...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nickol JM,Felsenfeld G

更新日期：1988-04-01 00:00:00

abstract：:The effect of 12-O-tetradecanoylphorbol 13-acetate (TPA), a potent tumor promoter, on the synthesis of proteins in BALB/c 3T3 mouse fibroblasts has been studied. When [35S]methionine-labeled proteins synthesized after the addition of TPA were analyzed by one- or two-dimensional polyacrylamide gel electrophoresis, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hiwasa T,Fujimura S,Sakiyama S

更新日期：1982-03-01 00:00:00

abstract：:The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fu YH,Marzluf GA

更新日期：1990-07-01 00:00:00

abstract：:Sexual arousal is a dynamical, highly coordinated neurophysiological process that is often induced by visual stimuli. Numerous studies have proposed that the cognitive processing stage of responding to sexual stimuli is the first stage, in which sex differences occur, and the divergence between men and women has been ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitricheva E,Kimura R,Logothetis NK,Noori HR

更新日期：2019-07-30 00:00:00

abstract：:India's northward flight and collision with Asia was a major driver of global tectonics in the Cenozoic and, we argue, of atmospheric CO(2) concentration (pCO(2)) and thus global climate. Subduction of Tethyan oceanic crust with a carpet of carbonate-rich pelagic sediments deposited during transit beneath the high-pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kent DV,Muttoni G

更新日期：2008-10-21 00:00:00

abstract：:Progress in ecosystem service science has been rapid, and there is now a healthy appetite among key public and private sector decision makers for this science. However, changing policy and management is a long-term project, one that raises a number of specific practical challenges. One impediment to broad adoption of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Polasky S,Tallis H,Reyers B

更新日期：2015-06-16 00:00:00

abstract：:Pre-mRNA splicing requires the function of a number of RNA-dependent ATPases/helicases, yet no three-dimensional structure of any spliceosomal ATPases/helicases is known. The highly conserved DECD-box protein UAP56/Sub2 is an essential splicing factor that is also important for mRNA export. The expected ATPase/helicas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi H,Cordin O,Minder CM,Linder P,Xu RM

更新日期：2004-12-21 00:00:00

abstract：:There is significant variation in the reported magnitude and even the sign of [Formula: see text] shifts in thin polymer films with nominally the same chemistry, film thickness, and supporting substrate. The implicit assumption is that methods used to estimate [Formula: see text] in bulk materials are relevant for inf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang W,Douglas JF,Starr FW

更新日期：2018-05-29 00:00:00

abstract：:In diabetes, the death of insulin-producing beta-cells by apoptosis leads to insulin deficiency. The lower prevalence of diabetes in females suggests that female sex steroids protect from beta-cell injury. Consistent with this hypothesis, 17beta-estradiol (estradiol) manifests antidiabetic actions in humans and rodent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le May C,Chu K,Hu M,Ortega CS,Simpson ER,Korach KS,Tsai MJ,Mauvais-Jarvis F

更新日期：2006-06-13 00:00:00

abstract：:The ability to infect macrophages is a common characteristic shared among many mycobacterial species. Mycobacterium avium, Mycobacterium tuberculosis, and Mycobacterium kansasii enter macrophages, using the complement receptors CR1, CR3, CR4, and the mannose receptor. To identify M. avium genes and host cell pathways ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Danelishvili L,Wu M,Stang B,Harriff M,Cirillo SL,Cirillo JD,Bildfell R,Arbogast B,Bermudez LE

更新日期：2007-06-26 00:00:00

abstract：:Tropical birds are relatively long-lived and produce few offspring, which develop slowly and mature relatively late in life, the slow end of the life-history axis, whereas temperate birds lie at the opposite end of this continuum. We tested the hypothesis that tropical birds have evolved a reduced basal metabolic rate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiersma P,Muñoz-Garcia A,Walker A,Williams JB

更新日期：2007-05-29 00:00:00

abstract：:HIV-1 envelope glycoprotein is the primary target for HIV-1-specific antibodies. The native HIV-1 envelope spike on the virion surface is a trimer, but trimeric gp140 and monomeric gp120 currently are believed to induce comparable immune responses. Indeed, most studies on the immunogenicity of HIV-1 envelope oligomers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovacs JM,Nkolola JP,Peng H,Cheung A,Perry J,Miller CA,Seaman MS,Barouch DH,Chen B

更新日期：2012-07-24 00:00:00

abstract：:The mitogenic neuropeptides bombesin and vasopressin markedly increased tyrosine and serine phosphorylation of multiple substrates in quiescent Swiss 3T3 fibroblasts, including two major bands of Mr 90,000 and 115,000. Tyrosine phosphorylation of these proteins was increased as judged by immunoprecipitation of 32Pi-la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zachary I,Gil J,Lehmann W,Sinnett-Smith J,Rozengurt E

更新日期：1991-06-01 00:00:00

abstract：:Magnetic field fluctuations arising from fundamental spins are ubiquitous in nanoscale biology, and are a rich source of information about the processes that generate them. However, the ability to detect the few spins involved without averaging over large ensembles has remained elusive. Here, we demonstrate the detect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann S,Simpson DA,Hall LT,Perunicic V,Senn P,Steinert S,McGuinness LP,Johnson BC,Ohshima T,Caruso F,Wrachtrup J,Scholten RE,Mulvaney P,Hollenberg L

更新日期：2013-07-02 00:00:00

abstract：:The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme. The complete coding regions, the 5'- and 3'-flanking regions, and the exon-intron...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyazaki H,Fukamizu A,Hirose S,Hayashi T,Hori H,Ohkubo H,Nakanishi S,Murakami K

更新日期：1984-10-01 00:00:00

abstract：:An isoform of the mammalian renal type II Na/Pi-cotransporter is described. Homology of this isoform to described mammalian and nonmammalian type II cotransporters is between 57 and 75%. Based on major diversities at the C terminus, the new isoform is designed as type IIb Na/Pi-cotransporter. Na/Pi-cotransport mediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hilfiker H,Hattenhauer O,Traebert M,Forster I,Murer H,Biber J

更新日期：1998-11-24 00:00:00

abstract：:We show that bromoacetaldehyde, which reacts selectively at the N-1 and N-6 positions of unpaired adenine and at the N-3 and N-4 positions of unpaired cytosine residues reacts with chromosomal DNA in intact cells at probable regulatory sequences near active genes. A region of about 200 base pairs 5' to the chicken bet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohwi-Shigematsu T,Gelinas R,Weintraub H

更新日期：1983-07-01 00:00:00

abstract：:Organic agriculture promotes sustainability compared to conventional agriculture. However, the multifunctional sustainability benefits of organic farms might be mediated by landscape context. Assessing how landscape context affects sustainability may aid in targeting organic production to landscapes that promote high ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith OM,Cohen AL,Reganold JP,Jones MS,Orpet RJ,Taylor JM,Thurman JH,Cornell KA,Olsson RL,Ge Y,Kennedy CM,Crowder DW

更新日期：2020-02-11 00:00:00

abstract：:Antibodies were raised against two synthetic peptides whose sequences correspond respectively to the COOH-terminal end (residues 501-516) of the protein encoded by the gene for the delta chain and to a proposed cytoplasmic region (residues 350-358) of the beta chain of the acetylcholine receptor from Torpedo californi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Young EF,Ralston E,Blake J,Ramachandran J,Hall ZW,Stroud RM

更新日期：1985-01-01 00:00:00

abstract：:Mounting effective T cell responses is critical for eliciting long-lasting immunity following viral infection and vaccination. A multitude of inhibitory and stimulatory factors are induced following infection, and it is the compilation of these signals that quantitatively and qualitatively program the ensuing effector...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brooks DG,Walsh KB,Elsaesser H,Oldstone MB

更新日期：2010-02-16 00:00:00

abstract：:No microorganism capable of anaerobic growth on methane as the sole carbon source has yet been cultivated. Consequently, information about these microbes has been inferred from geochemical and microbiological observations of field samples. Stable isotope analysis of lipid biomarkers and rRNA gene surveys have implicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orphan VJ,House CH,Hinrichs KU,McKeegan KD,DeLong EF

更新日期：2002-05-28 00:00:00

abstract：:The liver plays a critical role in inducing systemic immune tolerance, for example, during limiting hypersensitivity to food allergy and in rendering acceptance of allotransplant or even hepatotropic pathogens. We investigated the unknown mechanisms of liver tolerance by using an established hepatitis B virus (HBV)-ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu L,Yin W,Sun R,Wei H,Tian Z

更新日期：2013-10-15 00:00:00

abstract：:The extracellular matrix (ECM) is an intricate network composed of an array of macromolecules capable of regulating the functional responsiveness of cells. Its composition greatly varies among different types of tissue, and dysregulation of its metabolism may contribute to vascular remodeling during the pathogenesis o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roth M,Eickelberg O,Kohler E,Erne P,Block LH

更新日期：1996-05-28 00:00:00