Abstract:
:To study the influence of the non alpha-helical tail domain of keratins in filament formation, we prepared a truncated keratin 8 mutant, K8/tailless. Using site-directed in vitro mutagenesis we introduced a stop codon in the position coding for amino acid number 417 of the K8/wild-type sequence, thereby deleting 86 amino acids of the non alpha-helical tail domain but leaving the consensus sequence at the end of the rod domain intact. Expression of the truncated keratin 8 in Escherichia coli allowed us to purify the protein by a two-step procedure. The filament-forming capacity of the truncated K8 with wild-type K18 and K19 was analyzed using in vitro reconstitution. The in vitro assembly studies with K8/tailless and K18 wild-type indicate that the C-terminal tail domain of a type II keratin, including the homologous subdomain H2, is not required for filament formation. Moreover, reconstitution experiments with K8/tailless and K19, a naturally occurring tailless keratin I, show that the tail domains of type I as well as type II keratins are not an essential requirement for in vitro filament formation. Our results suggest that in vitro filament elongation does not depend on interactions between head and tail domains, although the tail domain might have a role in stabilization of intermediate filaments arising from certain keratin pairs.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Hatzfeld M,Weber Ksubject
Has Abstractpub_date
1990-10-01 00:00:00pages
317-24eissn
0021-9533issn
1477-9137journal_volume
97 ( Pt 2)pub_type
杂志文章abstract::The 5T4 oncofoetal antigen is a 72 kDa glycoprotein defined by a monoclonal antibody raised against human placental trophoblast and is expressed in many different carcinomas but detected only at low levels in some normal epithelia. Immunohistochemical analysis of the patterns of expression in colorectal carcinomas has...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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doi:
更新日期:1985-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1986-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-07-30 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-12-21 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.189068
更新日期:2017-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.133538
更新日期:2013-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.235390
更新日期:2019-12-13 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2007-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2004-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02609
更新日期:2005-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1978-04-01 00:00:00