Abstract:
:Although the human telomerase reverse transcriptase (hTERT) promoter can regulate cancer-specific genes, it is generally too weak to be effective. We therefore attempted to improve the potency of synthetic hTERT promoters by fusing the core element (E) of the hTERT promoter (H) and the tripartite leader sequence (T) from human adenovirus 5 in a combinatorial manner. To determine the potential as cancer-specific promoters, we measured luciferase activity driven by the chimeric hTERT promoters in human cancer cells. Among various constructs, the E3-H-T promoter induced the strongest luciferase activity in all the tested cancer cells. SK-Hep1 and Hela cells experienced 1000- and 11-fold higher expression than the basic hTERT promoter, respectively. Relative to the SV40 universal promoter, the E3-H-T promoter led to higher levels of gene expression. Using EMSA, we found that the hTERT enhancer region was specifically bound to c-Myc and Sp1. Thus, the data suggest that the E3-H-T promoter with up-regulated cancer-specific gene expression could be useful in cancer gene therapy.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Kim SJ,Lee HS,Shin JH,Kim CG,Jeong S,Park K,Choe H,Lee Hsubject
Has Abstractpub_date
2006-11-01 00:00:00pages
975-9issue
5eissn
1021-335Xissn
1791-2431journal_volume
16pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Survivin, a member of the inhibitor-of-apoptosis family is an essential protein for regular mitosis and is involved in an anti-apoptotic pathway. In some studies, an association between survivin expression and radiosensitivity has been described for tumor cells, but the relationship between p53 and survivin regarding ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The aim of this study was to define the clinical implications of semi-quantitative telomerase activity in gynecological tumors by comparing the telomerase activity of cancerous lesion and the adjacent non-cancerous lesion. In 118 cases of gynecologic tumors, including 41 uterine cervical tumors, 43 uterine body tumors...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.7.5.1003
更新日期:2000-09-01 00:00:00
abstract::The abilities of 5,6-benzoflavone (5,6-BF, a synthetic flavonoid), indole-3-carbinol (I3C, a plant derived product) or diindolylmethane (DIM, a condensation product of I3C) to alter the induction of mammary cancers induced by the carcinogens 7,12-dimethylbenzanthracene (DMBA) or N-methyl-N-nitrosourea (MNU) were evalu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1316
更新日期:2011-09-01 00:00:00
abstract::The interface between malignant melanoma and patient immunity has long been recognised and efforts to treat this most lethal form of skin cancer by activating immune responses with cytokine, vaccine and also antibody immunotherapies have demonstrated promise in limited subsets of patients. In the present study, we dis...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2014.3275
更新日期:2014-09-01 00:00:00
abstract::The aim of the present study was to discover the signaling pathways associated with 2-(3-hydroxy-5-methoxy-phenyl)-6,7-methylenedioxyquinolin-4-one (YYK1)-induced apoptosis in HL-60 human leukemia cells. YYK1 induced cytotoxic effects, cell morphological changes, decreased the cell ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1923
更新日期:2012-10-01 00:00:00
abstract::Somatic inactivation of the von Hippel-Lindau (VHL) gene is the most frequent genetic event observed in clear cell renal cell carcinoma (CC-RCC). However, the prognostic relevance of somatic VHL alteration and its target, hypoxia inducible factor (HIF)-1alpha has not been defined. We investigated the genetic changes i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::We examined pathomorphologically small nodular lesions in hepatitis C virus-related cirrhosis (type C cirrhosis). Small nodular lesions seen in the non-cancerous areas of 128 consecutively resected hepatocellular carcinomas (HCCs) associated with type C cirrhosis were selected for the study. From these, 18 large regen...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Fecal microRNAs (miRNAs) are increasingly explored as non-invasive markers of colorectal cancer (CRC). However, its holistic profile in Asian CRC patients remains elusive. In the present study, the global human fecal miRNAs in Asian Chinese CRC patients was assayed to elucidate novel diagnostic fecal markers. Addition...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3193
更新日期:2014-07-01 00:00:00
abstract::Pancreatic cancer is the fourth most common cause of cancer-related mortality. In the present study, we employed 2 types of therapeutic stem cells expressing cytosine deaminase (CD) with or without human interferon-β (IFN‑β), HB1.F3.CD and HB1.F3.CD.IFN-β cells, respectively, to selectively treat pancreatic cancer. Th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2564
更新日期:2013-09-01 00:00:00
abstract::Dysregulated metabolism is an emerging hallmark of cancer development, and upregulated lipid synthesis is one of the important tumor metabolic features. However, lipolysis may also contribute to cancer pathogenesis by altering free fatty acid (FFA) metabolism. In the present study, we investigated the importance of th...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3155
更新日期:2014-06-01 00:00:00
abstract::PTEN and the PI3K/Akt pathway are involved in the development and/or progression of endometrial carcinoma. To clarify the impact of the pathway-related molecules on prognosis, we analyzed PTEN, phosphorylated-Akt (p-Akt), and Ki-67 expression by immunohistochemistry in 99 patients with advanced endometrial carcinoma. ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::The t(8;21) translocation is one of the most frequent chromosome abnormalities associated with acute myeloid leukaemia (AML). This abberation deregulates numerous molecular pathways including the ERK signalling pathway among others. Therefore, the aim of the present study was to investigate the gene expression pattern...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6926
更新日期:2019-03-01 00:00:00
abstract::Diallyl disulfide (DADS) has shown potential as a therapeutic agent in various cancers. Previously, we found that myeloid cell leukemia sequence 1 (Mcl1) was downregulated in DADS-induced cell cycle arrest in HL-60 human leukemia cells. Here, we investigated the role of this protein...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1704
更新日期:2012-06-01 00:00:00
abstract::Melanoma is the most aggressive cutaneous cancer due to its propensity to metastasise and proliferate. Melanoma accounts for 80‑90% of skin‑cancer related deaths worldwide. Alhough numerous published studies have attempted to define the markers of diagnosis and prognosis of melanoma, a sensitive and specific biomarker...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6356
更新日期:2018-06-01 00:00:00
abstract::The MTA1 gene has been identified as metastasis-associated gene and has been seen to correlate with the degree of invasion and lymphatic metastasis in gastric, colorectal, and esophageal carcinomas. We investigated the possible role of MTA1 gene expression in hepatocellular carcinoma (HCC). The mRNA expression level o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::Antineoplastons are naturally occurring peptides and amino acid derivatives found in human blood and urine. antineoplastons have been shown to control neoplastic growth. In the present study, we investigated demethylation effect of the antineoplaston AS2-1 (a mixture of phenylacetylglutamine and phenylacetate in the r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2839
更新日期:2014-01-01 00:00:00
abstract::The characteristics of ovarian cancers that showed low activation of glycolysis were investigated. Using medical records of patients with ovarian cancers who had undergone fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) prior to their primary surgery at the University of Tokyo Hospital...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5398
更新日期:2017-03-01 00:00:00
abstract::Given the important role of CXCR4 in cancer metastasis, microenvironmental factors that modulate CXCR4 may have an impact on the process of tumor expansion. Hypoxia is a common feature of solid tumors and a significant microenvironmental factor that drives aggressive behavior. CXCR4...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2063
更新日期:2012-12-01 00:00:00
abstract::The S100A2 gene has been reported to be a putative tumor‑suppressor gene. Nevertheless, overexpression of S100A2 has been found in certain types of cancer. This study investigated S100A2 expression in tissue specimens of gastritis, intestinal metaplasia, adenomatous dysplasia and ga...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2236
更新日期:2013-04-01 00:00:00
abstract::Mammary small cell carcinoma (SmCC) is a very rare neoplasm with a poor prognosis compared with other invasive carcinomas. We studied the histological and immunohistochemical profiles of two cases of mammary SmCC, and compared them with those of five cases of carcinoma with endocrine features (CEF) and five cases of i...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00
abstract::Patients with autosomal recessive primary microcephaly have a small but architecturally normal brain containing a reduced number of neurons. Microcephalin and ASPM are two of the genes causing this disease. Both are centrosomal proteins involved in cell cycle regulation. Whereas microcephalin is a component of the DNA...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::Berbamine is an herbal compound derived from Berberis amurensis, which is used in Chinese traditional medicine. However, few studies have investigated this anti-tumor effect or the underlying mechanisms of berbamine on lymphoma cells. We investigate the effect, as well as the mechanism of action, of 4-chlorobenzoyl be...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000688
更新日期:2010-03-01 00:00:00
abstract::Cisplatin (CDDP)‑based combination chemotherapy is the standard for muscle‑invasive bladder cancer (MIBC). However, nearly all patients undergoing CDDP chemotherapy become refractory due to the development of CDDP resistance. Therefore, clarification of the mechanisms of CDDP resistance is urgently needed. The transcr...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7039
更新日期:2019-05-01 00:00:00
abstract::We present a case of resected hepatocellular carcinoma (HCC) which invaded the gallbladder with a metastasis to a lymph node. It was extremely difficult to make a differential diagnosis between HCC and gallbladder cancer preoperatively. A 68-year old man was admitted to hospital with complaint of a fever. Ultrasonogra...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.8.2.331
更新日期:2001-03-01 00:00:00
abstract::The present study was designed to retrospectively examine the efficacy of postoperative adjuvant chemotherapy in 107 patients with stage II primary colorectal cancer who underwent curative resection. The chemotherapy regimen was intravenous 5FU/LV in 30 patients (FL-IV group) and oral UFT/PSK in 77 patients (oral grou...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Cervical cancer is the second most common gynecological malignancy. The mechanisms of the genesis and progression of cervical cancer are complicated and not thoroughly understood. DEK is reported as an oncogene in various cancers, such as acute myeloid leukemia, bladder cancer, breast cancer and hepatocellular cancer....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5721
更新日期:2017-08-01 00:00:00
abstract::Oral lichen planus (OLP) is a chronic inflammatory disease that may transform to oral squamous cell carcinoma (OSCC), while its carcinogenesis mechanisms are not entirely clear. This study was designed to identify the important genes involved in the malignant transformation of OLP to OSCC. After RNA-sequencing, the di...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5487
更新日期:2017-04-01 00:00:00
abstract::Glioblastoma (GBM) is the most common type of primary central nervous system tumor in adults, which has high mortality and morbidity rates, and short survival time, namely <15 months after the diagnosis and application of standard therapy, which includes surgery, radiation therapy and chemotherapy; thus, novel therape...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7472
更新日期:2020-03-01 00:00:00
abstract::The p53 tumor suppressor is a transcription factor that regulates cell cycle and apoptosis in response to a variety of environmental stress conditions. Recent studies indicated that the apoptotic protease activating factor-1 (Apaf-1) is a direct transcriptional target of p53 in DNA damage-induced apoptosis. However, t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::The presence of hypoxic regions within solid tumors is caused by an imbalance between cell proliferation and angiogenesis. Such regions may facilitate the onset of recurrence after radiation therapy and chemotherapy, as hypoxic cells show resistance to these treatments. We found that tempol, a nitroxide, strongly indu...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4020
更新日期:2015-08-01 00:00:00