Abstract:
OBJECTIVE:Chondrocytes are highly sensitive to variations in extracellular glucose and oxygen levels in the extracellular matrix. As such, they must possess a number of mechanisms to detect and respond to alterations in the metabolic state of cartilage. In other organs such as the pancreas, heart and brain, such detection is partly mediated by a family of potassium channels known as K(ATP) (adenosine 5'-triphosphate-sensitive potassium) channels. Here we investigate whether chondrocytes too express functional K(ATP) channels, which might, potentially, serve to couple metabolic state with cell activity. METHODS:Immunohistochemistry was used to explore K(ATP) channel expression in equine and human chondrocytes. Biophysical properties of equine chondrocyte K(ATP) channels were investigated with patch-clamp electrophysiology. RESULTS:Polyclonal antibodies directed against the K(ATP) Kir6.1 subunit revealed high levels of expression in human and equine chondrocytes mainly in superficial and middle zones of normal cartilage. Kir6.1 was also detected in superficial chondrocytes in osteoarthritic (OA) cartilage. In single-channel electrophysiological studies of equine chondrocytes, we found K(ATP) channels to have a maximum unitary conductance of 47 +/- 9 pS (n=5) and a density of expression comparable to that seen in excitable cells. CONCLUSION:We have shown, for the first time, functional K(ATP) channels in chondrocytes. This suggests that K(ATP) channels are involved in coupling metabolic and electrical activities in chondrocytes through sensing of extracellular glucose and intracellular adenosine triphosphate (ATP) levels. Altered K(ATP) channel expression in OA chondrocytes may result in impaired intracellular ATP sensing and optimal metabolic regulation.
journal_name
Osteoarthritis Cartilagejournal_title
Osteoarthritis and cartilageauthors
Mobasheri A,Gent TC,Nash AI,Womack MD,Moskaluk CA,Barrett-Jolley Rdoi
10.1016/j.joca.2006.06.017subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1-8issue
1eissn
1063-4584issn
1522-9653pii
S1063-4584(06)00205-6journal_volume
15pub_type
杂志文章abstract:OBJECTIVE:To describe changes to the cancellous structure of femoral bone from patients with severe primary osteoarthritis by comparison with age and sex matched controls. METHOD:Specimens were taken from 18 male and 18 female pairs. One of each pair was a normal control, the other having severe primary osteoarthritis...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.1998.0141
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:To study the effect of 17β-estradiol (E2) and the superficial zone (SFZ) on cell death and proteoglycan degradation in articular cartilage after a single injurious compression in vitro. METHOD:Cartilage explants from the femoropatellar groove of 2 year old cows with or without the SFZ were cultured serum-fre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.07.007
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To study the effect of intra-articular injection of meloxicam (Mobic) on the development of osteoarthritis (OA) in rats and examine concomitant changes in nociceptive behavior and the expression of mitogen-activated protein kinases (MAPKs) in articular cartilage chondrocytes. METHODS:OA was induced in Wistar...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.09.005
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVES:Age-related changes in articular cartilage are likely to play a role in the aetiology of osteoarthritis (OA). One of the major age-related changes in cartilage is the accumulation of advanced-glycation-endproducts (AGEs). Since, cartilage tissue is not readily available from patients for studying AGE levels,...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,多中心研究
doi:10.1016/j.joca.2010.07.006
更新日期:2010-10-01 00:00:00
abstract:PURPOSE:This article reviews original publications related to imaging in osteoarthritis (OA) published in English from September 2011 through March 2012. In vitro data and animal studies are not covered. METHODS:To extract relevant studies, an extensive PubMed database search was performed using the query terms "osteo...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2012.07.008
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:The objective of this work was to compare the measurement properties of three categorical X-ray scoring methods for hip osteoarthritis (OA). METHODS:In data obtained from trials and cohorts, radiographs were evaluated using the Kellgren and Lawrence (KL) grading system, the Osteoarthritis Research Society In...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.06.009
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:We evaluated C-reactive protein (C-RP), a quantitative marker of the acute phase response, as a potential biomarker of prevalent and incident osteoarthritis of the knee (OAK). METHODS:Serum C-reactive protein concentrations were characterized with ultrasensitive rate nephelometry in a population-based sample...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1053/joca.2002.0800
更新日期:2002-08-01 00:00:00
abstract:OBJECTIVE:To investigate the in vivo role of the IRE1/XBP1 unfolded protein response (UPR) signaling pathway in cartilage. DESIGN:Xbp1(flox/flox).Col2a1-Cre mice (Xbp1(CartΔEx2)), in which XBP1 activity is ablated specifically from cartilage, were analyzed histomorphometrically by Alizarin red/Alcian blue skeletal pre...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2015.01.001
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVE:To compare the 2D and 3D MOCART system obtained with 9.4 T high-field magnetic resonance imaging (MRI) for the ex vivo analysis of osteochondral repair in a translational model and to correlate the data with semiquantitative histological analysis. METHODS:Osteochondral samples representing all levels of repa...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.05.027
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To examine the correlation between synovial fluid cartilage markers and degree of cartilage damage determined by arthroscopic evaluation in subjects with acute knee injury. DESIGN:Chondral damage was quantified using a validated arthroscopic scoring system in 20 subjects with effusive acute knee injuries of ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/s1063-4584(97)80046-5
更新日期:1997-11-01 00:00:00
abstract:OBJECTIVE:Until now there have been no appropriate models for metacarpophalangeal osteoarthritis (OA), even though OA in this joint is a significant medical and economic problem in horses. A good model would be useful to evaluate progression and treatment of OA, particularly in this joint. Therefore, we translated the ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2007.11.010
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVE:Although SOX9 is a key molecule for chondrogenic differentiation, little is known about the upstream signal. The present study attempted to identify transcription factors to induce SOX9 expression and examined the mechanism. METHODS:Sequences of about 1 kb of 5'-end flanking regions were compared between hum...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2009.02.003
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:To examine whether urine concentrations of type II collagen neoepitope (uTIINE) distinguish subjects with progressive radiographic and/or symptomatic knee osteoarthritis (OA) from those with stable disease. METHODS:Subjects were 120 obese middle-aged women with unilateral knee OA who participated in a 30-mon...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2006.04.009
更新日期:2006-11-01 00:00:00
abstract:OBJECTIVE:We aimed to (1) determine reference values for trochlear morphology and patellofemoral (PF) alignment in adults without magnetic resonance imaging (MRI)-defined PF full thickness cartilage damage or knee pain; and (2) evaluate dose-response patterns for these measures with prevalent MRI-defined PF structural ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2017.06.005
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to assess the reproducibility of meniscus T1rho measurements, and to study T1rho relaxation time in the lateral meniscus (LM) and its relationship with adjacent cartilage T1rho in knees with acute anterior cruciate ligament (ACL) injuries at 3T magnetic resonance imaging. METHOD:Qua...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2008.05.016
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:Obesity is a highly cited risk factor for knee osteoarthritis (OA), but its role in knee OA pathogenesis and progression is not as clear. Excess weight may contribute to an increased mechanical burden and altered dynamic movement and loading patterns at the knee. The objective of this study was to examine the...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2012.08.004
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVE:To resolve uncertainty regarding sex differences in osteoarthritis (OA) by performing a meta-analysis of sex differences in OA prevalence, incidence and severity. METHODS:Standard search strategies for population-based studies of OA providing sex-specific data. Random effects meta-analysis to provide pooled ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,meta分析
doi:10.1016/j.joca.2005.04.014
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the morphological changes of the lateral meniscus in end-stage lateral compartment osteoarthritis (OA) of the knee. METHODS:One hundred fifty-eight knee joints from 133 patients that subsequently underwent total knee joint arthroplasty from January 2008 to December 2009 ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2011.11.005
更新日期:2012-02-01 00:00:00
abstract:OBJECTIVE:C-C chemokine receptor type 5 (CCR5) has been implicated in rheumatoid arthritis and several inflammatory diseases, where its blockade resulted in reduced joint destruction. However, its role in modulating cartilage and bone changes in post-traumatic osteoarthritis (OA) has not yet been investigated. In this ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.12.002
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the site-dependent changes in the structure and function of articular cartilage in the lapine knee joint at a very early stage of osteoarthritis (OA), created experimentally by anterior cruciate ligament transection (ACLT). METHODS:Unilateral ACLT was performed in eig...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2014.04.010
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns. These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown. The objective was to identify differences in osteo...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2019.07.017
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:Summarize the comparative effectiveness of oral non-steroidal anti-inflammatory drugs (NSAIDs) and opioids in reducing knee osteoarthritis (OA) pain. METHODS:Two reviewers independently screened reports of randomized controlled trials (RCTs), published in English between 1982 and 2015, evaluating oral NSAIDs...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/j.joca.2016.01.135
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:To evaluate whether baseline T1ρ and T2 relaxation times of hip cartilage are associated with magnetic resonance imaging (MRI) based progression of hip osteoarthritis (OA) at 18 months. METHODS:3T MRI studies of the hip were obtained at baseline and 18-month follow-up for 54 subjects without evidence of seve...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.03.005
更新日期:2016-08-01 00:00:00
abstract::This paper reviews, in a first part, methods used for the clinical assessment of osteoarthritis (OA) with special reference, in a second part, to trials of drugs claimed to be chondromodulating agents. The agents examined include glycosaminoglycan-peptide complex (GP-C, Rumalon) and glycosaminoglycan-polysulfate (GAGP...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,评审
doi:10.1016/s1063-4584(05)80002-0
更新日期:1994-03-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare the early repair response of cartilage defects in trochlea (TR) and medial femoral condyle (MFC) at 2-3 weeks after bone marrow stimulation. DESIGN:Bilateral full-thickness cartilage defects were generated in central trochlear groove and MFC of skeletally mature rabbits. ...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2013.04.010
更新日期:2013-07-01 00:00:00
abstract:OBJECTIVE:Physical activity (PA) in the US knee osteoarthritis (OA) population is low, despite well-established health benefits. PA program implementation is often stymied by sustainability concerns. We sought to establish parameters that would make a short-term (3-year efficacy) PA program a cost-effective component o...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2020.01.017
更新日期:2020-06-01 00:00:00
abstract:OBJECTIVE:The Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) is applied extensively to patients with osteoarthritis of the hip or knee. Previous work has challenged the validity of its physical function scale however an extensive evaluation of its pain scale has not been reported. Our purpose wa...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2006.09.005
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:Exercise is vital for maintaining cartilage integrity in healthy joints. Here we examined the exercise-driven transcriptional regulation of genes in healthy rat articular cartilage to dissect the metabolic pathways responsible for the potential benefits of exercise. METHODS:Transcriptome-wide gene expression...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2016.02.004
更新日期:2016-07-01 00:00:00
abstract:PURPOSE:The number of effective knee osteoarthritis (OA) interventions, especially those tailored to specific compartmental involvement, are small. The objective of this study was to determine the efficacy of a realigning patellofemoral (PF) brace in improving pain and function among persons with symptomatic lateral PF...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.joca.2010.12.010
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVES:To determine the natural history of cartilage damage and of osteoarthritis (OA) progression using magnetic resonance imaging (MRI); to evaluate whether OA progression varies by stage of disease. METHODS:A population-based cohort with knee pain was assessed clinically, with X-ray (Kellgren-Lawrence [KL] grad...
journal_title:Osteoarthritis and cartilage
pub_type: 杂志文章
doi:10.1016/j.joca.2011.02.008
更新日期:2011-06-01 00:00:00