Abstract:
:WAVE1--the Wiskott-Aldrich syndrome protein (WASP)--family verprolin homologous protein 1--is a key regulator of actin-dependent morphological processes in mammals, through its ability to activate the actin-related protein (Arp2/3) complex. Here we show that WAVE1 is phosphorylated at multiple sites by cyclin-dependent kinase 5 (Cdk5) both in vitro and in intact mouse neurons. Phosphorylation of WAVE1 by Cdk5 inhibits its ability to regulate Arp2/3 complex-dependent actin polymerization. Loss of WAVE1 function in vivo or in cultured neurons results in a decrease in mature dendritic spines. Expression of a dephosphorylation-mimic mutant of WAVE1 reverses this loss of WAVE1 function in spine morphology, but expression of a phosphorylation-mimic mutant does not. Cyclic AMP (cAMP) signalling reduces phosphorylation of the Cdk5 sites in WAVE1, and increases spine density in a WAVE1-dependent manner. Our data suggest that phosphorylation/dephosphorylation of WAVE1 in neurons has an important role in the formation of the filamentous actin cytoskeleton, and thus in the regulation of dendritic spine morphology.
journal_name
Naturejournal_title
Natureauthors
Kim Y,Sung JY,Ceglia I,Lee KW,Ahn JH,Halford JM,Kim AM,Kwak SP,Park JB,Ho Ryu S,Schenck A,Bardoni B,Scott JD,Nairn AC,Greengard Pdoi
10.1038/nature04976subject
Has Abstractpub_date
2006-08-17 00:00:00pages
814-7issue
7104eissn
0028-0836issn
1476-4687pii
nature04976journal_volume
442pub_type
杂志文章相关文献
NATURE文献大全abstract::Cyclin degradation is the key step governing exit from mitosis and progress into the next cell cycle. When a region in the N terminus of cyclin is fused to a foreign protein, it produces a hybrid protein susceptible to proteolysis at mitosis. During the course of degradation, both cyclin and the hybrid form conjugates...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/349132a0
更新日期:1991-01-10 00:00:00
abstract::Brood parasitism, where a brood of the parasitic species is fostered by the parents of another species, is well known among birds. In most cases, such offspring show a complete reliance upon their host parents for food, protection and warmth until their independence. In other vertebrate groups, however, such total dep...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/323058a0
更新日期:1986-09-04 00:00:00
abstract::Since the discovery of insulin nearly 70 years ago, there has been no problem more fundamental to diabetes research than understanding how insulin works at the cellular level. Insulin binds to the alpha subunit of the insulin receptor which activates the tyrosine kinase in the beta subunit, but the molecular events li...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/352073a0
更新日期:1991-07-04 00:00:00
abstract::gamma-Aminobutyric acid (GABA) neurotransmission is widespread in vertebrate and invertebrate nervous systems. Here we use a genetic approach to identify molecules specific to GABA function. On the basis of the known in vivo roles of GABAergic neurons in controlling behaviour of the nematode Caenorhabditis elegans, we...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/364334a0
更新日期:1993-07-22 00:00:00
abstract::The nucleotide sequence of the RNA polymerase binding site at a promotor on fd RF DNA has been determined in comparison with the starting sequence of RNA initiated at this promoter. The RNA polymerase binding site contained the startpoint of transcription in the centre and a region with twofold symmetry in the non-tra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/253410a0
更新日期:1975-02-06 00:00:00
abstract::The mammalian central nervous system (CNS) contains a remarkable array of neural cells, each with a complex pattern of connections that together generate perceptions and higher brain functions. Here we describe a large-scale screen to create an atlas of CNS gene expression at the cellular level, and to provide a libra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02033
更新日期:2003-10-30 00:00:00
abstract::The development of cancer is contingent on the emergence of at least one clone of transformed cells. One method used to investigate whether human tumours are monoclonal depends on the mosaicism in the normal tissues of women heterozygous for the two forms of the enzyme glucose-6-phosphate dehydrogenase (G-6-PD). This ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/299822a0
更新日期:1982-10-28 00:00:00
abstract::Zika virus (ZIKV) is a flavivirus that is responsible for the current epidemic in Brazil and the Americas. ZIKV has been causally associated with fetal microcephaly, intrauterine growth restriction, and other birth defects in both humans and mice. The rapid development of a safe and effective ZIKV vaccine is a global ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature18952
更新日期:2016-08-25 00:00:00
abstract::Mitochondrial genomes (mitochondrial DNA, mtDNA) encode essential oxidative phosphorylation (OXPHOS) components. Because hundreds of mtDNAs exist per cell, a deletion in a single mtDNA has little impact. However, if the deletion genome is enriched, OXPHOS declines, resulting in cellular dysfunction. For example, Kearn...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature17989
更新日期:2016-05-19 00:00:00
abstract::The clinical success of the ABL tyrosine kinase inhibitor imatinib in chronic myeloid leukaemia (CML) serves as a model for molecularly targeted therapy of cancer, but at least two critical questions remain. Can imatinib eradicate leukaemic stem cells? What are the dynamics of relapse due to imatinib resistance, which...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03669
更新日期:2005-06-30 00:00:00
abstract::The origins of the building blocks of the Solar System can be studied using the isotopic composition of early planetary and meteoritic material. Oxygen isotopes in planetary materials show variations at the per cent level that are not related to the mass of the isotopes; rather, they result from the mixture of compone...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03947
更新日期:2005-09-15 00:00:00
abstract::Generalization is a cognitive ability that allows similar stimuli along a given dimension to be treated as equivalents. Insects exhibit high levels of visual generalization. Honeybees trained to recognize complex visual stimuli on the basis of a single feature generalize their choice to novel stimuli sharing that comm...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02594
更新日期:2004-06-17 00:00:00
abstract::Thermodynamics predicts that transmembrane voltage modulates membrane tension and that this will cause movement. The magnitude and polarity of movement is governed by cell stiffness and surface potentials. Here we confirm these predictions using the atomic force microscope to dynamically follow the movement of voltage...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35096578
更新日期:2001-09-27 00:00:00
abstract::Class-V myosin proceeds along actin filaments with large ( approximately 36 nm) steps. Myosin-V has two heads, each of which consists of a motor domain and a long (23 nm) neck domain. In accordance with the widely accepted lever-arm model, it was suggested that myosin-V steps to successive (36 nm) target zones along t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/415192a
更新日期:2002-01-10 00:00:00
abstract::Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons. Several groups have attributed this apparent plasticity to 'transdifferentiation'. Others, however, have suggested that cell fusion could explain these results...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02069
更新日期:2003-10-30 00:00:00
abstract::When an individual molecule, nanocrystal, nanotube or lithographically defined quantum dot is attached to metallic electrodes via tunnel barriers, electron transport is dominated by single-electron charging and energy-level quantization. As the coupling to the electrodes increases, higher-order tunnelling and correlat...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature00790
更新日期:2002-06-13 00:00:00
abstract::Design-specific control over excited-state dynamics is necessary for any application seeking to convert light into chemical potential. Such control is especially desirable in iron(II)-based chromophores, which are an Earth-abundant option for a wide range of photo-induced electron-transfer applications including solar...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2353-2
更新日期:2020-06-01 00:00:00
abstract::In fission yeast, meiosis is initiated by transcriptional activation of the mei3+ gene, under the combined influence of the four mating-type genes. The product of the mei3+ gene acts as a critical meiotic inducer by binding non-covalently to a newly identified protein kinase encoded by the ran1+ gene and inhibiting it...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/332509a0
更新日期:1988-04-07 00:00:00
abstract::The neurotrophin receptor TrkB is essential for normal function of the mammalian brain. It is expressed in three splice variants. Full-length receptors (TrkB(FL)) possess an intracellular tyrosine kinase domain and are considered as those TrkB receptors that mediate the crucial effects of brain-derived neurotrophic fa...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01983
更新日期:2003-11-06 00:00:00
abstract::The enzyme cytochrome c nitrite reductase catalyses the six-electron reduction of nitrite to ammonia as one of the key steps in the biological nitrogen cycle, where it participates in the anaerobic energy metabolism of dissimilatory nitrate ammonification. Here we report on the crystal structure of this enzyme from th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/22802
更新日期:1999-07-29 00:00:00
abstract::High biodiversity has been shown to enhance ecological stability on small spatial scales and over intervals of weeks to decades. It remains unclear, however, whether this diversity-stability relationship can be scaled up to regional scales, or to longer timescales. Without empirical validation at larger scales, the im...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature03152
更新日期:2005-01-27 00:00:00
abstract::T cells recognize foreign proteins as peptides bound to self molecules encoded by the major histocompatibility complex (MHC). The kinetics of interaction between purified class II MHC molecules and peptides is unusual, in that the rate of association is very slow, but once formed, the complexes are extremely stable. T...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/342800a0
更新日期:1989-12-14 00:00:00
abstract::A large impact by a comet or meteorite releases an enormous amount of energy, which evaporates, melts and fractures the surrounding rocks. Distinctive features of such impacts are 'shatter cones', deformed rocks characterized by hierarchical striated features. Although such features have been used for decades as unequ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature00903
更新日期:2002-07-18 00:00:00
abstract::An alphabeta T-cell response depends on the recognition of antigen plus major histocompatibility complex (MHC) proteins by its antigen receptor (TCR). The ability of peripheral alphabeta T cells to recognize MHC is at least partly determined by MHC-dependent thymic selection, by which an immature T cell survives only ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07812
更新日期:2009-04-23 00:00:00
abstract::The realization of superfluidity in a dilute gas of fermionic atoms, analogous to superconductivity in metals, represents a long-standing goal of ultracold gas research. In such a fermionic superfluid, it should be possible to adjust the interaction strength and tune the system continuously between two limits: a Barde...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02199
更新日期:2003-12-04 00:00:00
abstract::Diseases of the vascular system result from a complex mixture of genetic and environmental factors. Data sets, technologies and strategies emanating from the human genome programme have been applied to the analysis of both rare single-gene and common multigenic vascular disorders. Genomic approaches including inter- a...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/35025236
更新日期:2000-09-14 00:00:00
abstract::Data from a rocket experiment show details which were not known from ground-based observations. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/226526a0
更新日期:1970-05-09 00:00:00
abstract::During the evolution of the beta-globin family gene in vertebrates, different globin genes acquired different developmental patterns of expression. In mammals, specific 'embryonic' beta-like globins are synthesized in the earliest erythroid cells, which differentiate in the yolk sac of the embryo. In most mammals the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/319685a0
更新日期:1986-02-20 00:00:00
abstract::Human-mediated translocation of species to areas beyond their natural distribution (which results in 'alien' populations1) is a key signature of the Anthropocene2, and is a primary global driver of biodiversity loss and environmental change3. Stemming the tide of invasions requires understanding why some species fail ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-019-1292-2
更新日期:2019-07-01 00:00:00
abstract::The innate immune defence of multicellular organisms against microbial pathogens requires cellular collaboration. Information exchange allowing immune cells to collaborate is generally attributed to soluble protein factors secreted by pathogen-sensing cells. Cytokines, such as type I interferons (IFNs), serve to alert...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12640
更新日期:2013-11-28 00:00:00