Abstract：
:The retrograde axonal transport mechanism of motor neurons has been exploited to deliver the gene encoding Glial cell line-derived neurotrophic factor (GDNF) into the central nervous system to provide trophic support following injury. A nonviral gene delivery system, consisting of a monoclonal antibody (MC192) that binds the neurotrophic receptor, p75(NTR), coupled to poly-L-lysine, was constructed and used to deliver the gene via a receptor-mediated mechanism. The MC192-poly-l-lysine/pGDNF complex was injected into the hind limb of newborn rats to allow gene expression within motor neurons prior to sciatic nerve transection. In adult rats, the gene delivery complex was administrated in gel foam placed on a transected hypoglossal nerve. We show that the delivered construct is internalized following binding to p75(NTR) and is transported into the brain and spinal cord, bypassing the blood-brain barrier. The presence of the GDNF transgene and its transcript could be detected for up to 8 weeks in spinal cord and brain stem. Expression of the GDNF protein rescued 38% of the targeted motor neurons 1 week postinjury in newborn rats while the survival rate in control group was below 12%. In adult rats, neuronal death induced by axotomy was almost completely reversed by the introduction of the transgene (95 +/- 3%). Thus, the significant functional outcomes of this novel gene delivery system are demonstrated both in postnatal and adult motor neurons.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Barati S,Hurtado PR,Zhang SH,Tinsley R,Ferguson IA,Rush RAdoi
10.1016/j.expneurol.2006.05.027subject
Has Abstractpub_date
2006-11-01 00:00:00pages
179-88issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(06)00331-1journal_volume
202pub_type
杂志文章abstract：:The monoclonal antibody Py has previously been shown to be a useful marker for subpopulations of neurons in the rat brain. However, the distribution of Py immunoreactivity in other regions of the CNS and PNS is not known. Here, we present a light and electron microscopic investigation into the distribution of Py immun...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6509
更新日期：1997-07-01 00:00:00
abstract：:In the present study, we investigated whether cultured astrocytes derived from adult neural precursor cells (NPCs) obtained from the subventricular zone (SVZ) of wobbler mice display metabolic traits of the wobbler astrocytes in situ and in primary culture. We also utilized NPC-derived astrocytes as a tool to investig...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2010.06.008
更新日期：2010-09-01 00:00:00
abstract：:Vasoactive intestinal peptide (VIP) is one of the neuropeptides showing the strongest up-regulation in the nociceptive pathway after peripheral nerve injury and has been proposed to support neuropathic pain. Nevertheless, the story may be more complicated considering the known suppressive effects of the peptide on the...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.03.019
更新日期：2017-07-01 00:00:00
abstract：:The anatomical distribution and the content of dendrite bundles were studied in the spinal cord of adult rats. We injected retrogradely transported Cholera Toxin subunit B into muscles in order to label motoneurons and their dendrites. Twenty-one muscles in the trunk and in extremities were selected which are involved...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1996.0004
更新日期：1996-01-01 00:00:00
abstract：:With the use of four independent cell markers and Brd-U birthdating we have charted the earliest stages of neuronal differentiation and migration in the developing rat central nervous system, including the cortex, spinal cord, and retina. One of the markers, the monoclonal antibody 2G12, labeled a large subpopulation ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1995.1031
更新日期：1995-07-01 00:00:00
abstract：:The aminergic and cholinergic fibers innervating the hippocampus reach their target regions via the dorsal (dorsal fornix, fimbria, and cingulum) and ventral routes. The plasticity of this innervation after lesions of the dorsal pathway was investigated in the septal, medial, and temporal regions of the adult rat hipp...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(87)90053-7
更新日期：1987-05-01 00:00:00
abstract：:The ectopic discharges observed in uninjured dorsal root ganglion (DRG) neurons following various lesions of spinal nerves have been attributed to functional alterations of voltage-gated sodium channels (VGSCs). Such mechanisms may be important for the development of neuropathic pain. However, the pathophysiology unde...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2010.11.017
更新日期：2011-02-01 00:00:00
abstract：:Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix (ECM) molecules that are widely expressed throughout the developing and adult CNS. In vitro studies demonstrate their potential to restrict neurite outgrowth, and it is believed that CSPGs also inhibit axonal regeneration after CNS injury in vivo. Prev...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(03)00087-6
更新日期：2003-08-01 00:00:00
abstract：:Twenty-eight-day-old (juvenile) rats were kindled with hourly stimulations to partial or fully kindled status. Adult rats were stimulated with hourly or daily stimulations. Alterations in [3H]dihydroalprenolol binding were determined 3 weeks after the last stimulation. We found that partially kindled, hourly stimulate...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(87)90006-9
更新日期：1987-01-01 00:00:00
abstract：:Vasoactive intestinal peptide (VIP) is a regulator of rodent embryogenesis during the period of neural tube closure. VIP enhanced growth in whole cultured mouse embryos; treatment with a VIP antagonist during embryogenesis inhibited growth and development. VIP antagonist treatment during embryogenesis also had permane...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2007.04.004
更新日期：2007-07-01 00:00:00
abstract：:Nitrosative stress contributes to nerve conduction slowing, thermal hypoalgesia, and impaired nitrergic innervation in animal models of Type 1 diabetes. The role for reactive nitrogen species in Type 2 diabetes-associated neuropathy remains unexplored. This study evaluated the role for nitrosative stress in functional...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2007.03.019
更新日期：2007-06-01 00:00:00
abstract：:Reactive synaptogenesis and terminal proliferation are known to occur in the dentate gyrus of the rat hippocampus following removal of specific afferents. In the present study we have examined the relation of synaptophysin immunoreactivity to the immunohistochemical staining pattern of GAP-43, a putative marker of neu...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(91)90169-d
更新日期：1991-08-01 00:00:00
abstract：:Organotypic cultures of the brain provide a unique opportunity to directly examine the regional vulnerability of specific brain regions like the hippocampus. Two well-characterized models of oxidative stress were used to examine the regional vulnerability of the hippocampus. Endogenous oxidative stress was induced by ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6673
更新日期：1998-01-01 00:00:00
abstract：:Microfilaments (MFs) play an important role in wound healing and other regenerative events. The purpose of this study was to characterize changes in the distribution of MFs in traumatized auditory epithelia and compare these changes between avian (regenerating) and mammalian (nonregenerating) ears. Chicks and guinea p...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(92)90217-e
更新日期：1992-01-01 00:00:00
abstract：:We conducted a quantitative study of the trochlear motoneurons and the myotube clusters in the corresponding superior oblique muscle of the Japanese quail, before, during, and after the period of normally occurring motoneuron degeneration. A ratio of approximately 1:1 between myotube clusters and neurons was observed ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90063-9
更新日期：1985-10-01 00:00:00
abstract：:Oxidative stress can contribute to many neurological disease processes. Because many events known to involve oxidative stress (infection, hemorrhage, brain trauma) are accompanied by hydrocephalus, the present study sought to evaluate the relationship between oxidative stress and the progression of hydrocephalus. Assa...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1998.6969
更新日期：1999-01-01 00:00:00
abstract：:Five subjects matched the force of a sustained isometric contraction with a contraction of the contralateral muscle. Estimates of the sustained constant forces increased with time despite the direction to disregard the increased effort associated with the fatiguing contraction. These instructions did not produce a not...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90278-9
更新日期：1983-08-01 00:00:00
abstract：:Decreased brain insulin signaling has been found recently in Alzheimer's disease (AD). Intranasal administration of insulin, which delivers the drug directly into the brain, improves memory and cognition in both animal studies and small clinical trials. However, the underlying mechanisms are unknown. Here, we treated ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.06.004
更新日期：2014-11-01 00:00:00
abstract：:Injection of lysosomal enzyme into cisternal or ventricular cerebrospinal fluid (CSF) has been carried out in 11 lysosomal storage disorder models, with each study demonstrating reductions in primary substrate and secondary neuropathological changes, and several reports of improved neurological function. Whilst acute ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2014.09.008
更新日期：2015-01-01 00:00:00
abstract：:Our previous study demonstrates that delayed (initiated 24h post injury) erythropoietin (EPO) therapy for traumatic brain injury (TBI) significantly improves spatial learning. In this study, we investigated the impact of inhibition of EPO treatment-mediated neurogenesis on spatial learning after experimental TBI. Youn...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2012.02.015
更新日期：2012-05-01 00:00:00
abstract：:Transplantation of genetically engineered cells can provide sustained focal delivery of naturally occurring molecules, including neurotransmitters and growth factors. We have engineered immortalized mouse cortical neurons and glia to deliver GABA by driving GAD(65) expression. Engineered cell lines showed GAD(65) mRNA...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7305
更新日期：2000-02-01 00:00:00
abstract：:Myasthenia gravis (MG) with antibodies against the acetylcholine receptor (AChR) is characterized by a chronic, fatigable weakness of voluntary muscles. The production of autoantibodies involves the dysregulation of T cells which provide the environment for the development of autoreactive B cells. The symptoms are cau...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2015.03.010
更新日期：2015-08-01 00:00:00
abstract：:Axons of retinal ganglion cells (RGCs) of adult cats were transected and regenerated by the transplantation of peripheral nerves. Two months after transplantation dendritic arbors of RGCs with regenerated axons (r-RGCs) were visualized with intracellular injections of horseradish peroxidase (HRP). The HRP-filled r-RGC...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1996.6345
更新日期：1997-01-01 00:00:00
abstract：:Locally generating new proteins in subcellular regions provide means to spatially and temporally modify protein content in polarized cells. Recent years have seen resurgence of the concept that axonal processes of neurons can locally synthesize proteins. Experiments from a number of groups have now shown that axonal p...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2009.08.011
更新日期：2010-05-01 00:00:00
abstract：:Ocular movements of alert cats were recorded by classical electronystagmography techniques during (a) vestibular stimulation (sinusoidal rotation of the cat in complete darkness), (b) optokinetic stimulation (sinusoidal rotation of the visual surroundings around the stationary cat), (c) additive visual-vestibular stim...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(83)90005-5
更新日期：1983-04-01 00:00:00
abstract：:Many reports indicate that GM1 ganglioside is effective in reducing CNS ischemic injury in animal models. These models employ invasive surgery to induce ischemic damage in otherwise healthy animals. The purpose of this study was to determine if the beneficial effects of GM1 could be generalized to Spontaneously Hypert...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1045
更新日期：1994-03-01 00:00:00
abstract：:GAP-43 is a neuronal protein whose synthesis is elevated during developmental and regenerative axon growth. We propose that one consequence of this increased synthesis may be the delivery of calmodulin-like proteins to the distal portions of the growing axon at an increased velocity; this is because calmodulin, which ...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/0014-4886(92)90243-j
更新日期：1992-01-01 00:00:00
abstract：:Olfactory ensheathing cells (OECs) are unique glia that support axon outgrowth in the olfactory system, and when used as cellular therapy after spinal cord injury, improve recovery and axon regeneration. Here we assessed the effects of combining OEC transplantation with another promising therapy, epidural electrical s...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.07.015
更新日期：2018-11-01 00:00:00
abstract：:Extracellular deposits of fibrillar beta-amyloid are a characteristic neuropathology of Alzheimer's disease (AD). We have developed a novel antibody to a hypothesized "older isomer" of the amyloid protein. This antibody, raised against a synthetic beta-amyloid peptide containing isoaspartic acid at position 7 (isoaspa...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1999.7058
更新日期：1999-06-01 00:00:00
abstract：:The study investigates if alpha-lipoic acid is neuroprotective against chemotherapy induced neurotoxicity, if mitochondrial damage plays a critical role in toxic neurodegenerative cascade, and if neuroprotective effects of alpha-lipoic acid depend on mitochondria protection. We used an in vitro model of chemotherapy i...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.08.013
更新日期：2008-12-01 00:00:00
