Abstract:
:The turning point between apoptosis and necrosis induced by hydrogen peroxide (H2O2) have been investigated using human T-lymphoma Jurkat cells. Cells treated with 50 microM H2O2 exhibited caspase-9 and caspase-3 activation, finally leading to apoptotic cell death. Treatment with 500 microM H2O2 did not exhibit caspase activation and changed the mode of death to necrosis. On the other hand, the release of cytochrome c from the mitochondria was observed under both conditions. Treatment with 500 microM H2O2, but not with 50 microM H2O2, caused a marked decrease in the intracellular ATP level; this is essential for apoptosome formation. H2O2-reducing enzymes such as cellular glutathione peroxidase (cGPx) and catalase, which are important for the activation of caspases, were active under the 500 microM H2O2 condition. Prevention of intracellular ATP loss, which did not influence cytochrome c release, significantly activated caspases, changing the mode of cell death from necrosis to apoptosis. These results suggest that ATP-dependent apoptosome formation determines whether H2O2-induced cell death is due to apoptosis or necrosis.
journal_name
Free Radic Resjournal_title
Free radical researchauthors
Saito Y,Nishio K,Ogawa Y,Kimata J,Kinumi T,Yoshida Y,Noguchi N,Niki Edoi
10.1080/10715760600632552subject
Has Abstractpub_date
2006-06-01 00:00:00pages
619-30issue
6eissn
1071-5762issn
1029-2470pii
R76685230428T137journal_volume
40pub_type
杂志文章abstract::Weaning mice were fed a diet supplemented with beef tallow (BT) or BT plus docosahexaenoic acid (DHA) containing 100 mg alpha-tocopherol/kg (alpha-Toc100) or 500 mg alpha-tocopherol/kg (alpha-Toc500) for 4 wk to modify membrane fatty acid unsaturation, and then were administered ferric nitrilotriacetate (Fe-NTA). The ...
journal_title:Free radical research
pub_type: 杂志文章
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journal_title:Free radical research
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journal_title:Free radical research
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abstract::To define the molecular mechanism(s) of carvedilol inhibition of lipid peroxidation we have utilized model systems that allow us to study the different reactions involved in this complex process. Carvedilol inhibits the peroxidation of sonicated phosphatidylcholine liposomes triggered by FeCl2 addition whereas atenolo...
journal_title:Free radical research
pub_type: 杂志文章
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journal_title:Free radical research
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doi:10.3109/10715762.2015.1087642
更新日期:2015-01-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760000300551
更新日期:2000-06-01 00:00:00
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journal_title:Free radical research
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更新日期:2003-07-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760701581740
更新日期:2007-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760100301191
更新日期:2001-12-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760601184819
更新日期:2007-05-01 00:00:00
abstract:BACKGROUND:The objective of the present study was to determine whether single administration of the antioxidant enzyme bovine superoxide dismutase (bSOD) after radiation therapy (RT) mitigates development of pulmonary toxicity in rats. METHODS:Female F344 rats (n = 60) were divided among six experimental groups: (1) R...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2015.1066501
更新日期:2015-10-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2013.853877
更新日期:2013-12-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.3109/10715769709097827
更新日期:1997-06-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715762.2017.1418982
更新日期:2018-01-01 00:00:00
abstract::Dihydrocalcein (H2-calcein) is recommended as a superior probe for intracellular radical (ROS) detection as different to dichlorodihydrofluorescein (H2-DCF), its oxidation product calcein is thought not to leak out of cells. We determined whether H2-calcein is a useful tool to measure ROS in vascular smooth muscle cel...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/10715760400022145
更新日期:2004-12-01 00:00:00
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journal_title:Free radical research
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doi:10.1080/10715760600978807
更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/10715769609149917
更新日期:1996-08-01 00:00:00
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journal_title:Free radical research
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
abstract::We have previously reported that H2O2-induced apoptosis in HL-60 human leukemia cells takes place in the presence of chloride, requires myeloperoxidase (MPO), and occurs through oxidative reactions involving hypochlorous acid and chloramines. We now report that when chloride is replaced by the pseudohalide thiocyanate...
journal_title:Free radical research
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
abstract::The COST Action CM1201 "Biomimetic Radical Chemistry" has been active since December 2012 for 4 years, developing research topics organized into four working groups: WG1 - Radical Enzymes, WG2 - Models of DNA damage and consequences, WG3 - Membrane stress, signalling and defenses, and WG4 - Bio-inspired synthetic stra...
journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.1080/10715762.2016.1248961
更新日期:2016-11-01 00:00:00
abstract::We investigated the effect of curcumin on liver injury in diabetic rats induced by streptozotocin (STZ) through modulation of endoplasmic reticulum stress (ERS) and unfolded protein response (UPR). Experimental diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg), and curcumin was given at 100 ...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715762.2014.999674
更新日期:2015-03-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章,评审
doi:10.3109/10715762.2014.953494
更新日期:2014-11-01 00:00:00
abstract::We have here investigated possible occurrence of bicarbonate-dependent, carbonate radical anion (CO(3)(•-))-driven tocopherol-mediated human LDL peroxidation (TMP) in vitro and in vivo. CO(3)(•-), generated in vitro by the SOD1/H(2)O(2)/bicarbonate system, readily promoted TMP, which was dependent on α-tocopherol and ...
journal_title:Free radical research
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更新日期:2012-11-01 00:00:00
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journal_title:Free radical research
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doi:10.1080/10715760100300231
更新日期:2001-03-01 00:00:00
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journal_title:Free radical research
pub_type: 杂志文章
doi:10.3109/10715769609149049
更新日期:1996-09-01 00:00:00
abstract::Hyperthermia-induced apoptosis and its enhancement in the presence of a temperature-dependent free radical initiator, 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH) were examined in human uterine cervical cancer cell lines, CaSki and HeLa. When both cell lines were treated with hyperthermia at 44 degrees C for 60...
journal_title:Free radical research
pub_type: 杂志文章
doi:10.1080/1071576031000088292
更新日期:2003-06-01 00:00:00