Targeted disruption of growth hormone receptor interferes with the beneficial actions of calorie restriction.

abstract：:The discovery of a steadily growing number of tumor antigens (TAs) has made generic, cell-free, peptide-based cancer vaccines a possible alternative to cytokine-transfected autologous cellular cancer vaccines. The major drawback of peptide vaccines, however, is the poor immunogenicity of peptides. It is commonly thoug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buschle M,Schmidt W,Zauner W,Mechtler K,Trska B,Kirlappos H,Birnstiel ML

更新日期：1997-04-01 00:00:00

abstract：:Most B lymphocytes in normal individuals express two classes of cell-surface immunoglobulins, IgM and IgD. The specificity of the two antigen receptors is identical since they are produced by transcription and differential splicing of the same variable region gene segment to the heavy-chain constant region gene segmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irsch J,Irlenbusch S,Radl J,Burrows PD,Cooper MD,Radbruch AH

更新日期：1994-02-15 00:00:00

abstract：:In the primary visual cortex (V1) of many mammalian species, neurons are spatially organized according to their preferred orientation into a highly ordered map. However, whether and how the various presynaptic inputs to V1 neurons are organized relative to the neuronal orientation map remain unclear. To address this i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang QF,Li H,Chen M,Guo A,Wen Y,Poo MM

更新日期：2018-05-29 00:00:00

abstract：:We have used a semiselective Hahn spin-echo sequence of the form (1331)-tau-(2662)-tau-AQ, delivered by a surface coil to obtain high-resolution 1H NMR spectra from the brains of intact dead rats. This sequence gave suppression of the tissue water resonance by a factor of 80,000 when tau = 68 ms. Delivery of a frequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hetherington HP,Avison MJ,Shulman RG

更新日期：1985-05-01 00:00:00

abstract：:Leukemia inhibitory factor (LIF) receptor is a cell surface receptor that mediates the actions of LIF and other IL-6 type cytokines through the formation of high-affinity signaling complexes with gp130. Here we present the crystal structure of a complex of mouse LIF receptor with human LIF at 4.0 A resolution. The str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huyton T,Zhang JG,Luo CS,Lou MZ,Hilton DJ,Nicola NA,Garrett TP

更新日期：2007-07-31 00:00:00

abstract：:In humans, the connection between sleep and mood has long been recognized, although direct molecular evidence is lacking. We identified two rare variants in the circadian clock gene PERIOD3 (PER3-P415A/H417R) in humans with familial advanced sleep phase accompanied by higher Beck Depression Inventory and seasonality s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang L,Hirano A,Hsu PK,Jones CR,Sakai N,Okuro M,McMahon T,Yamazaki M,Xu Y,Saigoh N,Saigoh K,Lin ST,Kaasik K,Nishino S,Ptáček LJ,Fu YH

更新日期：2016-03-15 00:00:00

abstract：:The cingulate gyri in 37 subjects with and without early dementia of the Alzheimer type (DAT) were studied by using MRI at 1.0 mm3 isotropic resolution. Groups were segregated into young controls (n = 10), age-matched normal controls (n = 10), very mild DAT (n = 8), and mild DAT (n = 9). By using automated Bayesian se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller MI,Hosakere M,Barker AR,Priebe CE,Lee N,Ratnanather JT,Wang L,Gado M,Morris JC,Csernansky JG

更新日期：2003-12-09 00:00:00

abstract：:The Arabidopsis HYL1 gene encodes a nuclear double-stranded RNA-binding protein. A knockout mutation of the hyl1 gene is recessive and pleiotropic, causing developmental abnormalities, increasing sensitivity to abscisic acid, and reducing sensitivity to auxin and cytokinin. We report that levels of several microRNAs (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han MH,Goud S,Song L,Fedoroff N

更新日期：2004-01-27 00:00:00

abstract：:Increased understanding of the precise molecular mechanisms involved in cell survival and cell death signaling pathways offers the promise of harnessing these molecules to eliminate cancer cells without damaging normal cells. Tyrosine kinase oncoproteins promote the genesis of leukemias through both increased cell pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurokawa M,Ito T,Yang CS,Zhao C,Macintyre AN,Rizzieri DA,Rathmell JC,Deininger MW,Reya T,Kornbluth S

更新日期：2013-02-05 00:00:00

abstract：:The linear ubiquitin assembly complex (LUBAC) comprises 3 components: HOIP, HOIL-1, and Sharpin, of which HOIP and HOIL-1 are both members of the RBR subfamily of E3 ubiquitin ligases. HOIP catalyses the formation of Met1-linked ubiquitin oligomers (also called linear ubiquitin), but the function of the E3 ligase acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelsall IR,Zhang J,Knebel A,Arthur JSC,Cohen P

更新日期：2019-07-02 00:00:00

abstract：:Thyrotropin-releasing hormone (TRH) binds to specific receptors on GH4C1 pituitary tumor cells. A fluorescently labeled analog of TRH was synthesized by coupling pGlu-His-ProNH(CH2)6NH2 to fluorescein isothiocyanate. The fluorescein-labeled peptide (FL-TRH) stimulated prolactin synthesis and release by GH4C1 cultures ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halpern J,Hinkle PM

更新日期：1981-01-01 00:00:00

abstract：:Il4 and Il13 encode the canonical T helper 2 (TH2) cytokines responsible both for promoting immune responses against extracellular pathogens and, when misregulated, causing allergic and autoimmune disease. The expression potential of these genes undergoes developmentally programmed repression and enhancement during co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baguet A,Bix M

更新日期：2004-08-03 00:00:00

abstract：:The biconcave disk shape and deformability of mammalian RBCs rely on the membrane skeleton, a viscoelastic network of short, membrane-associated actin filaments (F-actin) cross-linked by long, flexible spectrin tetramers. Nonmuscle myosin II (NMII) motors exert force on diverse F-actin networks to control cell shapes,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith AS,Nowak RB,Zhou S,Giannetto M,Gokhin DS,Papoin J,Ghiran IC,Blanc L,Wan J,Fowler VM

更新日期：2018-05-08 00:00:00

abstract：:The effect of combining hormonal treatment consisting of long-acting microcapsules of the agonist [D-Trp6]LH-RH (the D-tryptophan-6 analog of luteinizing hormone-releasing hormone) with the chemotherapeutic agent cyclophosphamide was investigated in the Dunning R-3327H rat prostate cancer model. Microcapsules of [D-Tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schally AV,Redding TW

更新日期：1985-04-01 00:00:00

abstract：:It has been controversial whether the cytochrome oxidase (CO)-dense blobs in primate primary visual cortex (V1) and CO-dense thin stripes in visual area 2 (V2) are parts of a cortical color-processing stream that is segregated from other functional streams. One of the key pieces of evidence for the segregated color st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Y,Felleman DJ

更新日期：2004-05-04 00:00:00

abstract：:Recessive N-ethyl-N-nitrosourea (ENU)-induced mutations recovered at the fitness-1 (fit1) locus in mouse chromosome 7 cause hematopoietic abnormalities, growth retardation, and shortened life span, with varying severity of the defects in different alleles. Abnormal iron distribution and metabolism and frequent scolios...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klebig ML,Wall MD,Potter MD,Rowe EL,Carpenter DA,Rinchik EM

更新日期：2003-07-08 00:00:00

abstract：:A proton NMR method is described for determining the orientation of a porphyrin within the heme pocket of a hemoprotein. The pattern of the hyperfine-shifted heme methyl resonances in low-spin ferric model compounds is demonstrated to characteristically reflect the position of a localized low-symmetry perturbation on ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： La Mar GN,Budd DL,Viscio DB,Smith KM,Langry KC

更新日期：1978-12-01 00:00:00

abstract：:We have studied ten phenotypically similar patients with complete androgen insensitivity. All of the patients tested had significantly elevated serum luteinizing hormone and plasma androgens within or above the normal adult male range. On the basis of specific dihydrotestosterone binding by skin fibroblasts, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amrhein JA,Meyer WJ 3rd,Jones HW Jr,Migeon CJ

更新日期：1976-03-01 00:00:00

abstract：:High-resolution mapping of quantitative trait loci (QTL) in animals has proved to be difficult because the large effect sizes detected in crosses between inbred strains are often caused by numerous linked QTLs, each of small effect. In a study of fearfulness in mice, we have shown it is possible to fine map small-effe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mott R,Talbot CJ,Turri MG,Collins AC,Flint J

更新日期：2000-11-07 00:00:00

abstract：:Small ubiquitin-like modifier (SUMO) modification is emerging as an important control in transcription regulation. Here, we show that CREB-binding protein (CBP), a versatile transcriptional coactivator for numerous transcription factors in response to diverse signaling events, can be modified by SUMO-1 at lysine resid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuo HY,Chang CC,Jeng JC,Hu HM,Lin DY,Maul GG,Kwok RP,Shih HM

更新日期：2005-11-22 00:00:00

abstract：:Transfer RNA is one of the most richly modified biological molecules. Biosynthetic pathways that introduce these modifications are underexplored, largely because their absence does not lead to obvious phenotypes under normal growth conditions. Queuosine (Q) is a hypermodified base found in the wobble positions of tRNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles ZD,McCarty RM,Molnar G,Bandarian V

更新日期：2011-05-03 00:00:00

abstract：:Cytochromes P450 play key roles in drug metabolism and disease by oxidizing a wide variety of natural and xenobiotic compounds. High-resolution crystal structures of P450cam bound to ruthenium sensitizer-linked substrates reveal an open conformation of the enzyme that allows substrates to access the active center via ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunn AR,Dmochowski IJ,Bilwes AM,Gray HB,Crane BR

更新日期：2001-10-23 00:00:00

abstract：:Recent studies have shown that genes involved in oxidative phosphorylation (OXPHOS) exhibit reduced expression in skeletal muscle of diabetic and prediabetic humans. Moreover, these changes may be mediated by the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mootha VK,Handschin C,Arlow D,Xie X,St Pierre J,Sihag S,Yang W,Altshuler D,Puigserver P,Patterson N,Willy PJ,Schulman IG,Heyman RA,Lander ES,Spiegelman BM

更新日期：2004-04-27 00:00:00

abstract：:The 23S twisted circular form of ColE(1) DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximately 24S. Treatment of this complex with pronase, trypsin, sodium dodecyl sulfate, Sarkosyl, or heat results in a conversion to a slower sediment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clewell DB,Helinski DR

更新日期：1969-04-01 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00

abstract：:We present evidence of rapid and reliable associative learning by the terrestrial mollusc, Limax maximus. Slugs were exposed once to a pairing of a highly attractive food odor (potato or carrot) and a saturated solution of quinidine sulfate, a bitter-tasting plant substance. In comparison with control slugs, the expos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sahley C,Gelperin A,Rudy JW

更新日期：1981-01-01 00:00:00

abstract：:Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (SAH) or 5'-deoxyadenosine (5'-dAdo) and l-methionine (l-Met). Viperin c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenwick MK,Li Y,Cresswell P,Modis Y,Ealick SE

更新日期：2017-06-27 00:00:00

abstract：:The brain continuously influences and perceives the physiological condition of the body. Related cortical representations have been proposed to shape emotional experience and guide behavior. Although previous studies have identified brain regions recruited during autonomic processing, neurological lesion studies have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo CC,Sturm VE,Zhou J,Gennatas ED,Trujillo AJ,Hua AY,Crawford R,Stables L,Kramer JH,Rankin K,Levenson RW,Rosen HJ,Miller BL,Seeley WW

更新日期：2016-04-26 00:00:00

abstract：:Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes. Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity. Here, an approximately 20-kDa caveolin-related protein, caveolin-2, was ide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scherer PE,Okamoto T,Chun M,Nishimoto I,Lodish HF,Lisanti MP

更新日期：1996-01-09 00:00:00

abstract：:We identified a viral IL-10 homolog encoded by an ORF (UL111a) within the human cytomegalovirus (CMV) genome, which we designated cmvIL-10. cmvIL-10 can bind to the human IL-10 receptor and can compete with human IL-10 for binding sites, despite the fact that these two proteins are only 27% identical. cmvIL-10 require...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kotenko SV,Saccani S,Izotova LS,Mirochnitchenko OV,Pestka S

更新日期：2000-02-15 00:00:00