Abstract:
:In order to develop a detection method for the rice pathogens Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli, the phylogeny of six plant-pathogenic Burkholderia species was analysed using the combined nucleotide sequences of gyrB and rpoD. B. plantarii, B. glumae and B. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. The high sequence similarity revealed a close phylogenetic relationship between B. glumae and B. plantarii. B. plantarii strains were divided into three subclusters comprising rice strains, whereas the single Vanda strain occupied a unique position in the phylogenetic tree. The gyrB and rpoD sequences of all B. glumae strains examined were highly conserved. In contrast, B. gladioli strains demonstrated a far greater sequence diversity, but this diversity did not correlate with pathovar, host plant or geographical origin of the strains. A multiplex-PCR protocol using specific primers from the gyrB sequences was designed that allowed the specific detection and identification of B. plantarii, B. glumae and B. gladioli in rice seeds infected with these pathogenic species.
journal_name
Int J Syst Evol Microbiolauthors
Maeda Y,Shinohara H,Kiba A,Ohnishi K,Furuya N,Kawamura Y,Ezaki T,Vandamme P,Tsushima S,Hikichi Ydoi
10.1099/ijs.0.64184-0subject
Has Abstractpub_date
2006-05-01 00:00:00pages
1031-1038issue
Pt 5eissn
1466-5026issn
1466-5034journal_volume
56pub_type
杂志文章abstract::The generic name Flaviaesturariibacter Kang, Chun, Seo, Kim and Jahng 2015, 2212 is malformed: being derived from the Latin noun aestuarium, …aestuarii… instead of …aesturarii… is required. Moreover, according to Appendix 9 of the Prokaryotic Code, a connecting vowel must be dropped when the following word element sta...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002256
更新日期:2017-09-01 00:00:00
abstract::The novel yeast species Candida leandrae is described based on eight isolates from decaying fruits of Leandra reversa Cogn. (Melastomataceae) in an Atlantic rainforest site in Brazil, one from a Convolvulaceae flower in Costa Rica and one from a drosophilid in Hawai'i. The strains differed in their colony morphology, ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63259-0
更新日期:2004-11-01 00:00:00
abstract::Two novel strains, J77-1T and J76-1, were isolated from farmland soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. These strains were non-sporulating, catalase-positive and oxidase-negative. J77-1T an...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002456
更新日期:2017-12-01 00:00:00
abstract::Two gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria, strains KMM 9015 and KMM 9018(T), were isolated from a sample of shallow sediment collected from the Sea of Japan. An analysis of the nearly complete 16S rRNA gene sequences showed that the isolates were very close to each other phylogenetical...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.025551-0
更新日期:2011-07-01 00:00:00
abstract::A novel thermophilic, sulfur-oxidizing Gram-negative bacterium, designated strain SS-5T, was isolated from the Calcite Hot Springs in Yellowstone National Park, USA. The cells were motile rods (1.2-2.8 microm long and 0.6-0.8 microm wide). The new isolate was a facultative heterotroph capable of using elemental sulfur...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.63708-0
更新日期:2005-11-01 00:00:00
abstract::A marine bacterial strain, designated MD2(T), was isolated from the damaged tissue of a hydrocoral, Millepora dichotoma, collected from the coral reef in the northern Red Sea, Gulf of Eilat, Israel. Strain MD2(T) was Gram-reaction-negative, rod-shaped and motile, and formed small, creamy and opaque colonies, 1-2 mm in...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.043976-0
更新日期:2013-05-01 00:00:00
abstract::A bacterial isolate, designated strain TS3(T), was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, in central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolam...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.034306-0
更新日期:2012-10-01 00:00:00
abstract::A Gram-stain-negative, rod-shaped and white-coloured bacterial strain, designated LL03(T), was isolated from hexachlorocyclohexane-contaminated soil at Spolana Neratovice, Czech Republic, where lindane was formerly produced. Strain LL03(T) was found to be a degrader of α-, γ- and δ-isomers of hexachlorocyclohexane, al...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.039834-0
更新日期:2013-02-01 00:00:00
abstract::Four obligately anaerobic, thermophilic, sulfate-reducing bacterial strains, designated TGE-P1(T), TDV(T), TGL-LS1 and TSL-P1, were isolated from thermophilic (operated at 55 degrees C) methanogenic sludges from waste and wastewater treatment. The optimum temperature for growth of all the strains was in the range 55-6...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.2008/000893-0
更新日期:2008-11-01 00:00:00
abstract::A Gram-staining-negative, rod-shaped and non-motile bacterium, designated CL-SS4T, was isolated from crude-oil-contaminated seawater from the west coast of Korea and was investigated by means of a polyphasic taxonomic approach. Strain CL-SS4T grew optimally at 25-30 degrees C, at pH 6.8-7.7 and in the presence of 2-3%...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.007708-0
更新日期:2009-08-01 00:00:00
abstract::A Gram-negative bacterium, designated P2(T), was isolated from the biofilm developed on the inner surface of an ultrapure cooling water system in a Hungarian power plant and was characterized by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P2(T) was affiliated with...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.019398-0
更新日期:2010-11-01 00:00:00
abstract::A novel methane-producing archaeon, designated strain HASU(T), was isolated from a lotus field. Cells were Gram-negative, non-motile, irregular cocci, 2-3 mum in diameter, and occurred singly. Growth was observed at 15-40 degrees C (optimum, 37 degrees C) and pH 6.5-7.5 (optimum, pH 7.0). The G+C content of the genomi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.003731-0
更新日期:2009-04-01 00:00:00
abstract::Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribe...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-50-4-1629
更新日期:2000-07-01 00:00:00
abstract::The strictly anaerobic gram-negative bacterium strain Fru22T grows at 50 degrees C in media containing up to 75 g NaCl l(-1). Hexoses and pentoses are fermented to equal molar amounts of acetate, propionate and succinate, and no CO2 is formed. An orange-red pigment similar to flexirrubin is produced during stationary ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/00207713-52-1-173
更新日期:2002-01-01 00:00:00
abstract::A Gram-negative, strictly aerobic, non-spore-forming bacterium, motile by means of single polar flagellum and rod-shaped, designated strain GR12-1(T), was isolated from soil of a ginseng field in Yeongju region, Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is related to memb...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64427-0
更新日期:2007-03-01 00:00:00
abstract::A Gram-negative bacterium, designated strain EMB71(T), was isolated from activated sludge used for enhanced biological phosphorus removal in a sequencing batch reactor. The cells of the isolate were facultatively aerobic, motile rods with single polar flagella. Growth was observed to occur at 15-35 degrees C (optimall...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.64629-0
更新日期:2007-05-01 00:00:00
abstract::A novel bacterium, designated strain 5YN1-3(T), was isolated from wetland peat collected from Yongneup, Korea. The bacterium was facultatively anaerobic, Gram-negative, yellow-coloured, rod-shaped, mesophilic and motile with one polar flagellum. The strain grew optimally at 30 degrees C, at pH 6.0-9.0 and with 0-1 % N...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.002840-0
更新日期:2009-05-01 00:00:00
abstract::A novel actinobacterial strain, designated Z1027T, was isolated from a soil sample collected near the Tuotuo River, Qinghai-Tibet Plateau (China). The strain exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The taxonomic position of strain Z1027T was determined using a polyphasic ap...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.001456
更新日期:2016-12-01 00:00:00
abstract::A Gram-stain-negative, rod-shaped (1.2-2.1 μm × 0.8-0.9 μm), flagellated and motile marine bacterium, designated MEBiC05461T, was isolated from a marine sponge inhabiting Micronesia. Strain MEBiC05461T was oxidase-negative and catalase-positive. Growth was observed at 8.0-35.6 °C (optimum 30.0 °C), at pH 5.0-9.0 (opti...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.000188
更新日期:2015-06-01 00:00:00
abstract::Multilocus sequence analysis (MLSA) protocols have been developed for species circumscription for many taxa. However, at present, no studies based on MLSA have been performed within any moderately halophilic bacterial group. To test the usefulness of MLSA with these kinds of micro-organisms, the family Halomonadaceae,...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.032938-0
更新日期:2012-03-01 00:00:00
abstract::A Gram-stain-negative, strictly aerobic, motile, ivory-coloured and rod-shaped bacterium (designated Gsoil 520T) isolated from ginseng cultivation soil was characterized by using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 520T was observed to grow optimally at 30 °C and pH 7.0 on Reasoner's ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.003843
更新日期:2020-03-01 00:00:00
abstract::A Gram-stain-positive, non-motile, rod-shaped, catalase-positive and oxidase-negative bacterium, designated YIM CS25T, was isolated from a soil sample collected from Turpan desert in Xinjiang Uyghur Autonomous Region, north-western China. The isolate grew at 15-40 °C, at pH 6.0-8.0 and with 0-6 % (w/v) NaCl. The phylo...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000986
更新日期:2016-05-01 00:00:00
abstract::In the present study we investigated the taxonomic status of 20 lactic acid bacteria (LAB) originating from packaged meat. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the genus Leuconostoc with Leuconostoc gelidum, Leuconostoc inhae and Leuconostoc gasicomitatum as the clos...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.058263-0
更新日期:2014-04-01 00:00:00
abstract::An anaerobic, thermophilic bacterium, strain SET IS-9(T), was isolated from an Icelandic hot spring. Cells of strain SET IS-9(T) are short, slightly curved, motile rods. The strain grows chemolithotrophically on CO, producing equimolar quantities of H(2) and CO(2). It also grows fermentatively on lactate or pyruvate i...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.030288-0
更新日期:2011-10-01 00:00:00
abstract::An extremely halophilic archaeal strain SP28T was isolated from the Gomso solar saltern, Republic of Korea. Cells of the new strain SP28T were pleomorphic and Gram stain negative, and produced red-pigmented colonies. These grew in medium with 2.5-4.5 M NaCl (optimum 3.1 M) and 0.05-0.5 M MgCl2 (optimum 0.1 M), at 25-5...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.002313
更新日期:2017-11-01 00:00:00
abstract::This listing of names of prokaryotes published in a previous issue of the IJSEM is provided as a service to bacteriology to assist in the recognition of new names and new combinations. This procedure was proposed by the Judicial Commission [Minute 11(ii), Int J Syst Bacteriol41 (1991), p. 185]. The names given herein ...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijsem.0.000936
更新日期:2016-03-01 00:00:00
abstract::A Gram-positive, non-motile, cream-coloured, curved or straight rod-shaped bacterial strain, DS-10(T), was isolated from soil of Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-10(T) grew optimally at 25 degrees C and pH 7.0-8.0 in the presence of 0.5-1.0 % (w/v) NaC...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65072-0
更新日期:2008-06-01 00:00:00
abstract::A detailed classification of a novel bacterial strain, designated F11(T), capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This Gram-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium was isolated from a sediment sample collected f...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.65472-0
更新日期:2008-03-01 00:00:00
abstract::Two bacterial strains, Fuji 18-1(T) and Fuji 23-1(T), were isolated from the blood of the small Japanese field mouse (Apodemus argenteus) and the large Japanese field mouse (Apodemus speciosus), respectively, specimens of which were captured in the forest of Mount Fuji, Japan. Phenotypic characterization (growth condi...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.011528-0
更新日期:2010-04-01 00:00:00
abstract::Based on a list of 205 names proposed in original articles in the International Journal of Systematic and Evolutionary Microbiology or cited in Validation Lists from January 2001 that are not in accordance with Rules 27(3) and 30 of the International Code of Nomenclature of Bacteria (the Code), the Judicial Commission...
journal_title:International journal of systematic and evolutionary microbiology
pub_type: 杂志文章
doi:10.1099/ijs.0.2008/005264-0
更新日期:2008-07-01 00:00:00