Abstract:
BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. METHODS:MAb MDX-010 was added to AML PBMC cultures in the presence of GM-CSF and IL-4, a previously reported AML-DC culture method of generating anti-AML T cells. T-cell activation and proliferation were examined thereafter. RESULTS:Addition of MDX-010 to GM-CSF/IL-4-conditioned AML-DC cultures induced a mean seven-fold increase in the numbers of autologous T cells compared with cultures without MDX-010 (P < 0.007). T cells stimulated by AML-DC with CTLA-4 blockade were significantly more cytotoxic towards autologous AML cells than those without MDX-010 (42 +/- 23% vs. 26 +/- 15%, E:T ratio of 20). T cells stimulated by AML-DC with CTLA-4 blockade had significantly greater proportions of T cells producing IFN-gamma in response to autologous AML cells than those cultured with AML-DC alone (10.7 +/- 4.7% vs. 4.5 +/- 2.4% for CD4+ IFN-gamma+ CD69+ and 9.8 +/- 4.1% vs. 4 +/- 2.1% for CD8+ IFN-gamma+ CD69+ with or without MDX-010; n = 7; P < 0.007, P < 0.003, respectively). DISCUSSION:CTLA-4 blockade enhances the activity and numbers of AML-reactive T cells that can be stimulated by autologous AML-DC and may enhance the efficacy of adoptive immunotherapy of AML.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Zhong RK,Loken M,Lane TA,Ball EDdoi
10.1080/14653240500499507subject
Has Abstractpub_date
2006-01-01 00:00:00pages
3-12issue
1eissn
1465-3249issn
1477-2566pii
N0M3522V56183065journal_volume
8pub_type
杂志文章相关文献
CYTOTHERAPY文献大全abstract::Mesenchymal stromal cells (MSCs) are multipotent cells derived from various tissues that can differentiate into several cell types. MSCs are able to modulate the response of immune cells of the innate and adaptive immune system. Because of these multimodal properties, the potential use of MSCs for immunotherapies is c...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2017.03.076
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653249.2011.651529
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:DS60 is a novel buoyant density solution, whose density has been adjusted to enrich PBSC from subjects who have been mobilized with cytokines alone, or cytokines plus chemotherapy. This report describes the use of BDS60 to enrich autologous PBSC that were used for hematological reconstitution after myeloabla...
journal_title:Cytotherapy
pub_type: 临床试验,杂志文章
doi:10.1080/146532400539134
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AIMS:Specific and effective therapy for prevention or reversal of bronchiolitis obliterans (BO) is lacking. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF) gene modified mesenchymal stromal cells (MSCs) on BO. METHODS:A mouse model of experimental BO was established by s...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2015.12.006
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The aim of this study was to investigate preservation of anti-leukemic activity and protection from opportunistic infections after transplantation of allogeneic + cells in patients with hematologic malignancies and bad prognosis. Methods Thirty-three patients [median age 42 years, range 23-55 years, diagnosi...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/14653240410005375
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:In vitro primary cultures of microvascular endothelial cells from endocrine pancreas are difficult to obtain, but can be a very helpful tool for studies of islet biology, transplantation and regenerative medicine. METHODS:We applied a protocol recently described for the isolation and culture of brain microv...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.10.005
更新日期:2017-01-01 00:00:00
abstract::Not all patients with Hodgkin's disease (HD) respond to standard chemotherapy and/or radiation, and hematopoietic stem-cell transplantation has been gaining increasing acceptance in the management of HD. Phase II and, to a lesser extent, phase III studies of high-dose chemotherapy and autologous stem cell transplantat...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/146532402320219754
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND AIMS:To assess the impacts of stem cell therapy on clinical outcomes in patients with non-ischemic cardiomyopathy (NICM). The effect of stem cell therapy on prognosis is unclear and controversial. METHODS:The authors performed a systematic review and meta-analysis of the effects of autologous stem cell tran...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2020.06.006
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND AIMS:Retinal degeneration (RD) is an inherited eye disease characterized by irreversible photoreceptor loss. Conventionally, the activation of the resident microglia is secondary to the disease. Stem cell-based therapy has recently made rapid progress in treating RD. Although it has been demonstrated that th...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.03.001
更新日期:2016-06-01 00:00:00
abstract::Cell therapy products are frequently developed and produced without incorporating cost considerations into process development, contributing to prohibitively costly products. Herein we contextualize individual process development decisions within a broad framework for cost-efficient therapeutic manufacturing. This roa...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1016/j.jcyt.2017.06.009
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND AIMS:Tissue engineering technology is a promising therapeutic strategy in peripheral nerve injury. Schwann cells (SCs) are deemed to be a vital component of cell-based nerve regeneration therapies. Many methods for producing SC-like cells derived from adipose-derived stromal cells (ADSCs) have been explored,...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2019.04.061
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been used to minimize and repair radiation-induced normal tissue injury in the intestine, salivary gland, liver, skin, lungs and cardiac muscle. This study investigated the ability of adipose tissue-derived MSCs (aMSCs) to minimize and/or repair single dose radiatio...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.06.008
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND AIMS:It is important to improve the migratory ability of dendritic cells (DCs) and to increase DC potency for successful DC-based cancer immunotherapy. The intracellular Ca(2+) signaling pathway has an important role on the regulation of DC migration. Our preliminary studies revealed that sarco/endoplasmic r...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2015.06.002
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND AIMS:Intrauterine adhesion (IUA) is a common uterine cavity disease characterized by the unsatisfactory regeneration of damaged endometria. Recently, stem cell transplantation has been proposed to promote the recovery process. Here we investigated whether human amniotic mesenchymal stromal cells (hAMSCs), a ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2017.02.003
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Although a preponderance of pre-clinical data demonstrates the immunosuppressive potential of mesenchymal stromal cells (MSCs), significant heterogeneity and lack of critical quality attributes (CQAs) based on immunosuppressive capacity likely have contributed to inconsistent clinical outcomes. This heteroge...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2018.10.008
更新日期:2019-01-01 00:00:00
abstract::Broader application of adoptive transfer of tumor-specific T-lymphocytes is accompanied by the need for effective suicide genes to ensure the safety of this cell-based therapy. In vivo elimination of T-lymphocytes expressing the herpes simplex virus-derived thymidine kinase gene has demonstrated the feasibility of thi...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240310001497
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND AIMS:The purpose of this study was to investigate the neuroprotective effects of bone marrow bone marrow-derived and adipose tissue-derived mesenchymal stromal cells (MSCs) that were intravitreally transplanted in an experimental ocular hypertension (OHT) model. METHODS:An OHT rat model was generated by mea...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2014.12.005
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND AIMS:Cytotherapy is a promising option for neurodegenerative disease treatment. Because of the fatal prognosis and imperative need for effective treatment, amyotrophic lateral sclerosis (ALS) patients request this therapy before its effectiveness has been verified. The increase in clinics offering cytotherap...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653241003774037
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND AIMS:Dendritic cell (DC)-based therapies could be important strategies for lymphoma treatment. METHODS:AKR/J mice with Dalton's lymphoma were treated with recombinant interleukin-15 (rIL-15)-activated autologous DCs and pulsed with whole tumor cell lysates in the presence or absence of suboptimal doses of t...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2015.01.006
更新日期:2015-05-01 00:00:00
abstract:BACKGROUND:Clinical studies have shown the efficacy of combination therapy for various malignancies. In this study, the characteristics, safety and feasibility of use of cascade-primed (CAPRI) cells for the combination treatment of non-small-cell lung cancer (NSCLC) were evaluated both in vitro and in vivo. METHODS:Si...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2019.12.002
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND AIMS:The aim of this study was to evaluate the efficacy and safety of cytokine-induced killer (CIK) cell therapy for solid carcinomas. METHODS:We performed a computerized search of phase II/III clinical trial databases of CIK cell-based therapy using a combination of the terms 'cytokine-induced killer cells...
journal_title:Cytotherapy
pub_type: 杂志文章,meta分析,评审
doi:10.3109/14653249.2011.649185
更新日期:2012-04-01 00:00:00
abstract::The efficacy of cord blood (CB) transplantation is limited by the low cell dose available. Low cell doses at transplant are correlated with delayed engraftment, prolonged neutropenia and thrombocytopenia and elevated risk of graft failure. To potentially improve the efficacy of CB transplantation, approaches have been...
journal_title:Cytotherapy
pub_type: 杂志文章,评审
doi:10.1080/14653240510027172
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:Mismatched family donor and unrelated donor BM transplants are associated with a high risk of acute GvHD. White T-cell depletion is the best method to reduce risk of acute GvHD, there was a reluctance to use T-cell depletion in the mismatched setting because of increased risk of rejection and relapse. Partia...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1080/0032472031000141284
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AIMS:Apoptosis of radiosensitive cells in the bone marrow and gut is a serious, at times life-threatening, complication arising from radiation exposure. METHODS:We investigated whether adoptive transfer of allogeneic bone marrow-derived mesenchymal stromal cells (MSC) could exert cytoprotective and life-spa...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.3109/14653249.2012.684378
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND AIMS:Mesenchymal stromal cells (MSCs), derived from several tissues including bone marrow and adipose tissue, are being evaluated in clinical trials for a range of diseases. Virtually all tissues of the body contain stromal cells, yet it is unknown whether these sources are similar in phenotype and function....
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2015.07.009
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND AIMS:Traditionally, stem cell therapy for myocardial infarction (MI) has been administered as a single treatment in the acute or subacute period after MI. These time intervals coincide with marked differences in the post-infarct myocardial environment, raising the prospect that repeat cell dosing could provi...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.07.016
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND AIMS:Cell therapy is promising as an exploratory cardiovascular therapy. We have recently developed an investigational new drug named Stempeucel (bone marrow-derived allogeneic mesenchymal stromal cells) for patients with acute myocardial infarction (AMI) with ST-segment elevation. A phase I/II randomized, d...
journal_title:Cytotherapy
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.jcyt.2014.10.009
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND AIMS:The graft-healing potential of mesenchymal stromal cells (MSCs) derived from the remnants of ruptured anterior cruciate ligaments (ACLs) after ACL reconstruction may depend on the chronicity of the injury. The aim of this study was to assess the quantitative and phenotypic differences between MSCs isola...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2013.08.001
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND AIMS:Immunomodulatory properties of human umbilical cord-derived mesenchymal stromal cells (UCMSCs) can be differentially modulated by toll-like receptors (TLR) agonists. Here, the therapeutic efficacy of short TLR3 and TLR4 pre-conditioning of UCMSCs was evaluated in a dextran sulfate sodium (DSS)-induced c...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2016.02.002
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Multiple myeloma (MM) is a hematologic cancer caused by the abnormal expansion of plasma cells, but the exact mechanism underlying MM development is not completely known. Recently, multiple long noncoding RNAs (lncRNAs) were implicated in the regulation of MM development. METHODS:Samples from patients with ...
journal_title:Cytotherapy
pub_type: 杂志文章
doi:10.1016/j.jcyt.2018.10.006
更新日期:2019-01-01 00:00:00