Abstract：
:The yellow fever mosquito Aedes aegypti has been the subject of extensive genetic research due to its medical importance and the ease with which it can be manipulated in the laboratory. A molecular genetic linkage map was constructed using 148 amplified fragment length polymorphism (AFLP) and six single-strand conformation polymorphism (SSCP) markers. Eighteen AFLP primer combinations were used to genotype two reciprocal F2 segregating populations. Each primer combination generated an average of 8.2 AFLP markers eligible for linkage mapping. The length of the integrated map was 180.9 cM, giving an average marker resolution of 1.2 cM. Composite interval mapping revealed a total of six QTL significantly affecting Plasmodium susceptibility in the two reciprocal crosses of Ae. aegypti. Two common QTL on linkage group 2 were identified in both crosses that had similar effects on the phenotype, and four QTL were unique to each cross. In one cross, the four main QTL accounted for 64% of the total phenotypic variance, and digenic epistasis explained 11.8% of the variance. In the second cross, the four main QTL explained 66% of the variance, and digenic epistasis accounted for 16% of the variance. The actions of these QTL were either dominance or underdominance. Our results indicated that at least three new QTL were mapped on chromosomes 1 and 3. The polygenic nature of susceptibility to P. gallinaceum and epistasis are important factors for significant variation within or among mosquito strains. The new map provides additional information useful for further genetic investigation, such as identification of new genes and positional cloning.
journal_name
Geneticsjournal_title
Geneticsauthors
Zhong D,Menge DM,Temu EA,Chen H,Yan Gdoi
10.1534/genetics.105.055178subject
Has Abstractpub_date
2006-07-01 00:00:00pages
1337-45issue
3eissn
0016-6731issn
1943-2631pii
genetics.105.055178journal_volume
173pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The vacuolar hydrolase protease B in Saccharomyces cerevisiae is synthesized as an inactive precursor (Prb1p). The precursor undergoes post-translational modifications while transiting the secretory pathway. In addition to N- and O-linked glycosylations, four proteolytic cleavages occur during the maturation of Prb1p....
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:The sequentially Markov coalescent is a simplified genealogical process that aims to capture the essential features of the full coalescent model with recombination, while being scalable in the number of loci. In this article, the sequentially Markov framework is applied to the conditional sampling distribution (CSD), ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.125534
更新日期：2011-04-01 00:00:00
abstract：:The parasitoid wasp Nasonia represents a genus of four species that is emerging as a powerful genetic model system that has made and will continue to make important contributions to our understanding of evolutionary biology, development, ecology, and behavior. Particularly powerful are the haplodiploid genetics of the...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.112.147512
更新日期：2015-04-01 00:00:00
abstract：:The availability of the complete DNA sequence of the Chlamydomonas reinhardtii genome and advanced computational biology tools has allowed elucidation and study of the small ubiquitin-like modifier (SUMO) system in this unicellular photosynthetic alga and model eukaryotic cell system. SUMO is a member of a ubiquitin-l...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.089128
更新日期：2008-05-01 00:00:00
abstract：:We analyzed the dynamics of transposable elements (TEs) according to Wright's island and continent-island models, assuming that selection tends to counter the deleterious effects of TEs. We showed that migration between host populations has no impact on either the existence or the stability of the TE copy number equil...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032243
更新日期：2005-01-01 00:00:00
abstract：:The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:We present here a molecular analysis of the region surrounding the structural gene encoding alcohol dehydrogenase (Adh) in 47 lines of Drosophila melanogaster that have each accumulated mutations for 300 generations. While these lines show a significant increase in variation of alcohol dehydrogenase enzyme activity co...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:The Sh2/A1 orthologous region of maize, rice, and sorghum contains five genes in the order Sh2, X1, X2, and two A1 homologs in tandem duplication. The Sh2 and A1 homologs are separated by approximately 20 kb in rice and sorghum and by approximately 140 kb in maize. We analyzed the fate of the Sh2/A1 region in large-ge...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Transcriptional silencing involves the formation of specialized repressive chromatin structures. Previous studies have shown that the histone H3-H4 chaperone known as chromatin assembly factor 1 (CAF-1) contributes to transcriptional silencing in yeast, although the molecular basis for this was unknown. In this work w...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.054783
更新日期：2006-06-01 00:00:00
abstract：:Genetic adaptation to external stimuli occurs through the combined action of mutation and selection. A central problem in genetics is to identify loci responsive to specific selective constraints. Many tests have been proposed to identify the genomic signatures of natural selection by quantifying the skew in the site ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.152587
更新日期：2013-09-01 00:00:00
abstract：:Selection of Gl-Sb coupling heterozygotes was carried out for more than one hundred generations commencing with six independent lines drawn from a common base population. Population sizes were eight, sixteen and forty-eight parents per generation. The effect of natural selection on recombination value was measured by ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-03-01 00:00:00
abstract：:G-protein-coupled receptors (GPCRs) are integral membrane proteins that initiate stimulus-dependent activation of cognate heterotrimeric G-proteins, triggering ensuing downstream cellular responses. Tight regulation of GPCR-evoked pathways is required because prolonged stimulation can be detrimental to an organism. St...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.186122
更新日期：2016-05-01 00:00:00
abstract：:An analysis of the effects of spontaneous mutations affecting age-specific mortality was conducted using 29 lines of Drosophila melanogaster that had accumulated spontaneous mutations for 19 generations. Divergence among the lines was used to estimate the mutational variance for weekly mortality rates and the covarian...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Scans of the human genome have identified many loci as potential targets of recent selection, but exploration of these candidates is required to verify the accuracy of genomewide scans and clarify the importance of adaptive evolution in recent human history. We present analyses of one such candidate, enamelin, whose p...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.077123
更新日期：2008-03-01 00:00:00
abstract：:Differential gene expression across cell types underlies development and cell physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates to the difficulty to obtain comprehensive tissue-specific gene transcri...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303774
更新日期：2020-12-01 00:00:00
abstract：:Meiotically driven sex chromosomes can quickly spread to fixation and cause population extinction unless balanced by selection or suppressed by genetic modifiers. We report results of genetic analyses that demonstrate that extreme female-biased sex ratios in two sister species of stalk-eyed flies, Cyrtodiopsis dalmann...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Retrotransposons are mobile genetic elements that colonize eukaryotic genomes by replicating through an RNA intermediate. As retrotransposons can move within the host genome, defense mechanisms have evolved to repress their potential mutagenic activities. In the fission yeast Schizosaccharomyces pombe, the mRNA of Tf2...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.193870
更新日期：2017-02-01 00:00:00
abstract：:We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of the canonical P sequence. Elements belonging to this repressor class were isolated from P strains and were made in vitr...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:The neutral rate of allelic substitution is analyzed for a class-structured population subject to a stationary stochastic demographic process. The substitution rate is shown to be generally equal to the effective mutation rate, and under overlapping generations it can be expressed as the effective mutation rate in new...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.163345
更新日期：2014-05-01 00:00:00
abstract：:Forty-eight new mutations at the am locus of Neurospora crassa have been characterized. Nineteen mutations were induced by UV; of these, eight were missense, two were frameshifts, two were nonsense, three were deletions and four were unidentified. Twenty-nine mutations were induced with nitrous acid; of these, twenty-...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:In the absence of a vg+ gene, extensive cell death occurs in third instar imaginal discs, which results in a complete loss of adult wing margin structures. Essentially all molecularly characterized vg alleles are associated with deletions or insertions of DNA into the vg locus. These alterations reduce or eliminate a ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Ten mutations are described that transform genotypic hermaphrodites of the nematode Caenorhabditis elegans into phenotypic males. These fall into three autosomal complementation groups, termed tra-1, tra-2, and tra-3. Two alleles of tra-1 produce almost complete transformation, to a fertile male phenotype; such transf...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:Whereas nonlinear relationships between genes are acknowledged, there exist only a few methods for estimating nonlinear gene coexpression networks or gene regulatory networks (GCNs/GRNs) with common deficiencies. These methods often consider only pairwise associations between genes, and are, therefore, poorly capable ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303186
更新日期：2020-07-01 00:00:00
abstract：:bld2-1 mutant Chlamydomonas reinhardtii strains assemble basal bodies with singlet microtubules; bld2-1 cells display flagellar assembly defects as well as positioning defects of the mitotic spindle and cleavage furrow. To further understand the role of the BLD2 gene, we have isolated three new bld2 alleles and three ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Biology is characterized by complex interactions between phenotypes, such as recursive and simultaneous relationships between substrates and enzymes in biochemical systems. Structural equation models (SEMs) can be used to study such relationships in multivariate analyses, e.g., with multiple traits in a quantitative g...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.112979
更新日期：2010-06-01 00:00:00
abstract：:Thirteen of 14 temperature-sensitive mutants deficient in successive steps of mitotic chromosome transmission (cdc2, 4, 5, 6, 7, 8, 9, 13, 14, 15, 16, 17 and 20) from spindle pole body separation to a late stage of nuclear division exhibited a dramatic increase in the frequency of chromosome loss and/or mitotic recomb...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmen...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.051144
更新日期：2006-02-01 00:00:00
abstract：:We report the results of a genetic screen designed to identify transcriptional coregulators of yeast heat-shock factor (HSF). This sequence-specific activator is required to stimulate both basal and induced transcription; however, the identity of factors that collaborate with HSF in governing noninduced heat-shock gen...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.052738
更新日期：2006-04-01 00:00:00
abstract：:Microsatellites have been widely used as tools for population studies. However, inference about population processes relies on the specification of mutation parameters that are largely unknown and likely to differ across loci. Here, we use data on somatic mutations to investigate the mutation process at 14 tetranucleo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
abstract：:Progress in understanding the evolution of variation at the MHC has been slowed by an inability to assess the relative roles of mutation vs. intragenic recombination in contributing to observed polymorphism. Recent theoretical advances now permit a quantitative treatment of the problem, with the result that the amount...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
