Abstract:
:Mechanism of interaction of non-steroidal anti-inflammatory drugs, celecoxib and valdecoxib with human serum albumin has been studied using fluorescence spectroscopic technique. There was only one high affinity site on serum albumin for both the drugs with association constants of the order of 10(4) in the case of celecoxib and 10(5) in the case of valdecoxib. Thermodynamic parameters for the binding indicated that hydrogen bonding interactions are predominantly involved in the binding of these drugs to human serum albumin. Binding studies in the presence of hydrophobic probe, 1-anilinonaphthalene-8-sulfonate (ANS) suggested that the mode of interaction of drugs and ANS with HSA is different and hydrophobic interactions are not primarily involved in the binding. Studies carried out in the presence of site-specific probe showed that drugs are bound at site II and phenolic oxygen of (411)Tyr is involved in binding. Stern-Volmer analysis of the quenching data indicated that predominantly static quenching mechanism is operative and the tryptophan residues of albumin are fully accessible to celecoxib and only partially accessible to valdecoxib. The presence of salt caused a decrease in the association constant and significant increase in the concentration of free drug.
journal_name
Pharmacol Resjournal_title
Pharmacological researchauthors
Seedher N,Bhatia Sdoi
10.1016/j.phrs.2006.02.008subject
Has Abstractpub_date
2006-08-01 00:00:00pages
77-84issue
2eissn
1043-6618issn
1096-1186pii
S1043-6618(06)00035-1journal_volume
54pub_type
杂志文章abstract::Bleomycin (BLM) is a drug used to treat different types of neoplasms. BLM's most severe adverse effect is lung toxicity, which induces remodeling of lung architecture and loss of pulmonary function, rapidly leading to death. While its clinical role as an anticancer agent is limited, its use in experimental settings is...
journal_title:Pharmacological research
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Pharmacological research
pub_type: 杂志文章
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journal_title:Pharmacological research
pub_type: 杂志文章,评审
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更新日期:2019-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.phrs.2016.09.008
更新日期:2016-11-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2004.02.008
更新日期:2004-09-01 00:00:00
abstract::Isoxanthohumol (IXN), a prenylated flavonoid from hops, exhibits diverse biological activities, e.g. antitumor, antiinflammatory, antioxidant and antiangiogenic. In this study, the effect of IXN is evaluated on two melanoma cell lines with dissimilar molecular background, B16 and A375. The treatment of both cell lines...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.01.011
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Thymidylate synthase (TS) expression levels appear to be related to response to 5-fluorouracil-(5-FU)-based chemotherapy in colorectal cancer (CRC) patients. Three polymorphisms have been proposed as modulators of TS expression: a tandemly repeated sequence (2R/3R) in the 5' UTR, a SNP (G>C) w...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2011.04.006
更新日期:2011-09-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2010.11.003
更新日期:2011-02-01 00:00:00
abstract::Curcumin, a bioactive polyphenol, is a yellow pigment of the Curcuma longa (turmeric) plant. Curcumin has many pharmacologic effects including antioxidant, anti-carcinogenic, anti-obesity, anti-angiogenic and anti-inflammatory properties. Recently, it has been found that curcumin affects lipid metabolism, and subseque...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2017.02.008
更新日期:2017-05-01 00:00:00
abstract::The molecular complexity of human breast cancer (BC) renders the clinical management of the disease challenging. Long non-coding RNAs (lncRNAs) are promising biomarkers for BC patient stratification, early detection, and disease monitoring. Here, we identified the involvement of the long intergenic non-coding RNA 0108...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2020.105249
更新日期:2020-11-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(89)80005-2
更新日期:1989-05-01 00:00:00
abstract::Most cancer cells generate energy through aerobic glycolysis to enable their rapid growth and proliferation, which is a phenomenon known as Warburg effect. Inhibition of aerobic glycolysis reduces lactate and ATP generation in cancer cells, and ultimately kills tumor cells. Increasing evidence suggests that wogonin, a...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2018.07.011
更新日期:2018-09-01 00:00:00
abstract::The most frequent cause of mortality and morbidity in industrialized countries is coronary artery disease (CAD), which in Europe alone is responsible for around two million deaths per year. In 2001 it accounted for about 260,000 hospital discharges in Italy. The costs of CAD treatment in Italy--which were borne by the...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2006.04.002
更新日期:2006-08-01 00:00:00
abstract::Inflammation plays a key role in tumor promotion and development. Indeed, cyclooxygenase-2 (COX-2) expression is strongly associated with different types of cancer. An emerging class of compounds with significant anti-inflammatory properties is the hydrogen sulfide-releasing non-steroidal anti-inflammatory drugs (H2S-...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2016.10.019
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journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1006/phrs.1997.0227
更新日期:1997-09-01 00:00:00
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journal_title:Pharmacological research
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doi:10.1016/j.phrs.2017.08.018
更新日期:2017-11-01 00:00:00
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journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2020.105252
更新日期:2020-11-01 00:00:00
abstract::The antiepileptic drug vigabatrin (GVG) is known to decrease significantly the serum concentration of concurrently administered phenytoin (PHT) in epileptic patients. To assess a possible mechanism for this interaction, the effect of GVG on the intestinal absorption of PHT was investigated by means of circulation expe...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/s1043-6618(05)80133-1
更新日期:1992-09-01 00:00:00
abstract::The aqueous extract from aerial parts of Artemisia copa Phil. (Compositae), was evaluated for antinociceptive activity using writhing, formalin, and hot-plate tests in mice. A dose-related antinociceptive response was obtained in the writhing test at doses of 500 and 1000 mg/kg p.o. (percentage of inhibition 23.3 and ...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2003.12.016
更新日期:2004-07-01 00:00:00
abstract::The metabolism of arachidonic acid by cytochrome P450 (CYP) epoxygenases generates epoxyeicosatrienoic acids (EETs) which affect numerous cellular process including Ca(2+) signaling and the activity of Ca(2+)-dependent K(+) channels. The expression of the CYP epoxygenase(s) that generate EETs in endothelial cells is n...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2003.11.016
更新日期:2004-06-01 00:00:00
abstract::Combination antiretroviral therapy reduces morbidity and mortality in HIV infected patients. However, the cure of HIV infection is hindered by the persistence of the latent HIV reservoir. Latency reversing agents (LRAs) are developed to target the HIV latently infected cells for HIV reactivation. In addition to revers...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2018.10.023
更新日期:2019-01-01 00:00:00
abstract::In the present study the effect of ketamine on the contractions caused by propoxur and phoxim on the isolated guinea pig ileum was investigated. Ketamine was found able to inhibit in a concentration-dependent manner the contractile responses of the ileum to propoxur and phoxim, while it did not significantly modify th...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/1043-6618(94)80003-0
更新日期:1994-08-01 00:00:00
abstract::Regulatory T cells (Tregs) modulate ongoing immune responses to prevent autoimmunity in healthy bodies and inhibit effective anti-tumor immunity responses in tumor patients, leading to tumor progression. The function of Tregs in tumor immunity suggests that elimination of Tregs in the host may enhance the anti-tumor i...
journal_title:Pharmacological research
pub_type: 杂志文章,评审
doi:10.1016/j.phrs.2020.104659
更新日期:2020-03-01 00:00:00
abstract::Heparin-binding protein (HBP; CAP37/azurocidin) is secreted from neutrophil leukocytes early during inflammation and plays a central role in early capillary leakage and extravasation of neutrophils. Furthermore, HBP is chemotactic towards monocytes and lymphocytes and protects against stress-induced apoptosis, e.g. in...
journal_title:Pharmacological research
pub_type: 杂志文章
doi:10.1016/j.phrs.2005.01.004
更新日期:2005-06-01 00:00:00