Effects of Terminalia arjuna bark extract on apoptosis of human hepatoma cell line HepG2.

Abstract:

AIM:To investigate the effects of Terminalia arjuna (T. arjuna) extract on human hepatoma cell line (HepG2) and its possible role in induction of apoptosis. METHODS:Human hepatoma cells were treated with different concentrations of ethanolic extract of T. arjuna and its cytotoxicity effect was measured by trypan blue exclusion method and lactate dehydrogenase leakage assay. Apoptosis was analyzed by light and fluorescence microscopic methods, and DNA fragmentation. The mechanism of apoptosis was studied with expression of p53 and caspase-3 proteins. Glutathione (GSH) content was also measured in HepG2 cells after T. arjuna treatment. RESULTS:T. arjuna inhibited the proliferation of HepG2 cells in a concentration-dependent manner. Apoptotic morphology was observed in HepG2 cells treated with T. arjuna at the concentrations of 60 and 100 mg/L. DNA fragmentation, accumulation of p53 and cleavage of procaspase-3 protein were observed in HepG2 cells after the treatment with T. arjuna. The depletion of GSH was observed in HepG2 cells treated with T. arjuna. CONCLUSION:T. arjuna induced cytotoxicity in HepG2 cells in vitro. Apoptosis of HepG2 cells may be due to the DNA damage and expression of apoptotic proteins. Depletion of GSH may be involved in the induction of apoptosis of HepG2 cells.

journal_name

World J Gastroenterol

authors

Sivalokanathan S,Vijayababu MR,Balasubramanian MP

doi

10.3748/wjg.v12.i7.1018

keywords:

subject

Has Abstract

pub_date

2006-02-21 00:00:00

pages

1018-24

issue

7

eissn

1007-9327

issn

2219-2840

journal_volume

12

pub_type

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