Abstract：
:Phospholipid composition of sheep blood platelets and its various plasma membrane fractions have been analyzed. Based on their flotation rates in discontinuous sucrose density gradient centrifugation, three membrane fractions were isolated. 5'-Nucelotidase and alkaline phosphatase were distributed nearly equally in all the three membrane fractions. However these membrane fractions showed differences in the distribution of phosphatidyl ethanolamine, phosphatidyl choline and phosphoinositides. Phosphatidyl ethanolamine was predominant in fraction I (11.05 micrograms PLP/mg protein) while phosphatidyl choline was predominant in fractions II and III (110.10 and 68.30 micrograms PLP/mg protein respectively). Phosphatidyl inositol (Ptd-InsP) was equally distributed in all three membrane fractions. However, both Ptd-InsP and phosphatidyl inositol 4,5-bisphosphate were about 4-fold higher in fraction II (73.55 and 89.89 micrograms PLP/mg protein respectively).
journal_name
Indian J Biochem Biophysjournal_title
Indian journal of biochemistry & biophysicsauthors
Kumar V,Srivastava N,Grover SK,Jaiswal AS,Bansal SK,Misra UKkeywords:
subject
Has Abstractpub_date
1991-04-01 00:00:00pages
133-5issue
2eissn
0301-1208issn
0975-0959journal_volume
28pub_type
杂志文章abstract：:In order to gain insight into the antioxidant effect of the cell extract from Bordetella pertussis (strain 18-323, phase I) pancreatic antioxidant enzymes, glutathione (GSH), lipid conjugated dienes, DNA strand breaks in islet cells and the in vitro ROM scavenging potential of the extract were studied in 18 hr-fasted ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Viscosity measurements have been performed on aqueous solutions of some solutes commonly used in biochemical practice, with a view to determine the B coefficients and their temperature dependence. The temperature dependence measured for some anions leads to a ranking of the latter in an order similar to that reported ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:The effect of methylglyoxal on protein -SH and -NH2 groups in cytosolic and membranous fractions of epithelial cells lining the gastrointestinal tract of rat was investigated, using isolated villus and crypt cells (enterocytes) and colonocytes. It was found that 11-12% cytosolic protein -SH and 14-17% membrane protein...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1990-02-01 00:00:00
abstract：:A transplantable, ER+ mammary carcinoma has been induced in virgin female Wistar rats by an 'air-pouch' technique by xenografting MCF7 cells. An air pouch induced by injecting sterile air subcutaneously into the mammary fat pad was primed with pristane and 17 beta estradiol (10 micrograms) in peanut oil prior to injec...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Hepcidin is an anti-microbial peptide expressed predominantly in the liver of many species. Based on the amino acid sequence deduced from buffalo (Bubalus bubalis) hepcidin cDNA (Accession no. EU399814), six peptides Hepc(1-25), Hepc(6-25), Hepc(7-25), Hepc(9-25), Hepc(11-25) and Hepc(15-25) were synthesized using sol...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：:In order to identify the complementary glycoproteins (receptors) that are recognized in bovine brain stem by endogenous 14 kDa galactose-binding lectin (BBL), probable glycoproteins were first selected by affinity chromatography of soluble tissue glycoproteins on Rinicus communis agglutinin (RCA)-Sepharose since this ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:N-Terminal analysis of purified buffalo thyroglobulin by the fluorodinitrobenzene method of Sanger yielded about 1.5 moles of DNP-glutamic acid per mole of buffalo thyroglobulin. No water-soluble DNP-amino acid was detectable as N-terminal. The presence of glutamic acid has been confirmed by Edman degradation and char...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:Drought-tolerant cultivars and their phytochemical composition, which has a role in providing drought tolerance are gaining importance. In this study, rice bran oil and semi-purified oryzanol (SPO) obtained from five rice (Oryza sativa L.) cultivars, namely P1401 and PB1 (drought-susceptible) and N22, PNR381 and APO (...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2014-02-01 00:00:00
abstract：:A relatively simple and rapid method is described for the isolation of basal cell membranes (BCM) from the human placenta at term which showed considerable improvement in the yield, purity and membrane characteristics as compared to the earlier described methods. The method is based on thorough washings of the syncyti...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Perturbative configuration interaction using localized orbital (PCILO) computations have been carried out on conformational preference of d(GpC) and d(CpG) units of Z-II DNA. By keeping the sugar pucker fixed in the crystallographic conformation, PCILO energies have been calculated as a function of torsion angles alph...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Oxidative stress is believed to play a central role in aging and age-associated diseases. It leads to oxidative changes in human red blood cells (RBCs) in vivo and in vitro. In this study, we evaluated the oxidative damage to the erythrocytes during aging in the humans using RBC as a model, by measuring the cytosolic ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2010-10-01 00:00:00
abstract：:C-reactive protein (CRP) is found to be a normal component of serum of freshwater air-breathing murrel Channa punctatus. Based on the property of binding with C-polysaccharide (CPS) of pneumococcus bacteria in presence of Ca2+, CRP was purified by phosphorylcholine-Sepharose affinity column chromatography. Molecular w...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The short-term action of thyroid hormone tri-iodothyronine (T3) was studied in vivo and in vitro on antioxidant enzyme activities in a teleost Anabas testudineus (Bloch). T3 injection in vivo (200 ng) in normal fish decreased the lipid peroxidation products and increased superoxide dismutase (SOD), catalase and glutat...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:The influence of dietary caloric intake was studied on blood pressure (BP), serum lipids, body mass index (BMI) and waist-hip-ratio (WHR) according to age and gender. The study population consisted of 400 healthy individuals as controls and 746 first time detected untreated hypertensive (HTN) subjects. The subjects we...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：:Using the isopiestic vapour pressure technique, the magnitudes of excess binding of water and NaCl per mole of twenty different poly-L-amino acid residues, respectively in the presence of different bulk molefractions (X2) of NaCl have been evaluated from the mathematical expressions for the Gibbs surface excesses. At ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:Antioxidant activity of ethanolic extract of fruits of Terminalia chebula (500 mg/kg body wt, orally for 30 days) against isoproterenol-induced oxidative stress was investigated in rats. The levels of serum lipid peroxides, iron, ascorbic acid, vitamin E, plasma iron-binding capacity, and the activities of ceruloplasm...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2005-08-01 00:00:00
abstract：:Wild progenitors of rice (Oryza) are an invaluable resource for restoring genetic diversity and incorporating useful traits back into cultivars. Studies were conducted to characterize the biochemical changes, including SDS-PAGE banding pattern of storage proteins in seeds of six wild species (Oryza alta, O. grandiglum...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：:Beta-adrenoceptors (β-AR), members of the G protein-coupled receptors play important roles in the regulation of heart function. A positive inotropic action of catecholamines is mediated through their interaction with β-AR, located on the sarcolemma, while they can also mediate some deleterious effects, such as cardiac...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2014-12-01 00:00:00
abstract：:R-spondin (Rspo)s proteins are a new group of Wnt/beta-catenin signaling agonists. These signaling molecules are known to be involved in the developmental stages of skeletal system. Recent studies in various murine osteoblast models have proposed that Rspo 1 may interact with Wnt signaling pathway to induce differenti...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2013-02-01 00:00:00
abstract：:Gold nanoparticles have diverse applications and are being used in food and cosmetic industry, for drug delivery and in the diagnosis and treatment of cancer. However there is a need to study their biochemical mode of action. In this study, in vivo effect of gold nanoparticles on the activities of the two antioxidant ...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:The rhizomes of Nardostachysjatamansi, the plant commonly known as Jatamansi have been described in Ayurveda for their soothing and sedative action on the central nervous system. In the present study, the anti-stress effect of hydroethanolic extract (70%) of N. jatamansi (NJE) was evaluated in reference to its antioxi...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:In vitro mutagenesis was used to produce two photosystem I mutants of the cyanobacterium Synechocystis sp. PCC 6803. The mutant HK and HL contained hexahistidyl tags at the C-termini of the PsaK1 and PsaL subunits, respectively. The HK mutant contained wild-type amounts of trimeric PS I complexes, but the level of hex...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:A simple kinetic model for the membrane-bound enzyme, 'Na+, K(+)-ATPase', is presented. An equation for the efflux of Na+ and influx of K+ is derived. This also explains multiple ligand binding of homospecies of these cations and competition between heterospecies of these cations. The equation is computed by a small B...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Several decades of research in biochemistry and molecular biology have been devoted for studies on isolated enzymes and proteins. Recent high throughput technologies in genomics and proteomics have resulted in avalanche of information about several genes, proteins and enzymes in variety of living systems. Though these...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2007-06-01 00:00:00
abstract：:In the Zajdela Ascitic Hepatoma (ZAH), a rat tumor, high levels of cell surface sialic acid residues are present which masked the immunogenicity of the cells. We have shown here that cell surface sialic acid level goes down rapidly when ZAH cells are put in culture. The reduction in surface sialic acid levels is due t...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1995-06-01 00:00:00
abstract：:Crystallins are a diverse group of proteins that constitute nearly 90% of the total soluble proteins of the vertebrate eye lens and these tightly packed crystallins are responsible for transparency of the lens. These proteins have been studied in different model and non-model species for understanding the modification...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2011-02-01 00:00:00
abstract：:Quantitative structure-activity relationship (QSAR) analysis was performed on a series of 5, 6-diarylthiazolo [3, 2-b]-1, 2, 4-triazoles to explore their possible interaction with the active amino acid residues of cyclooxygenase-2 (COX-2) enzyme. The significance of orientation and conformational rigidity of 5, 6-diar...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2004-08-01 00:00:00
abstract：:The report describes results of separation of sialyltransferase isoenzymes by electrofocusing plasma from healthy volunteers and patients having different types of malignant tumour. Extensive modification of the technique was adopted in determining enzyme activity, such as elution of gel strips with the buffer pH corr...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Therapeutic efficacy of liposomal Hamycin has been evaluated in an animal model system for aspergillosis in Balb/c mice. Hamycin was intercalated into soya phosphatidyl choline (SPC), SPC: choline (1:1, vol./vol.) and DMPC liposomes. A single dose of either 0.1 mg/kg, 0.25 mg/kg or 0.5 mg/kg of liposomal Hamycin and 0...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:The renal protective effect of an active principle isolated from the aqueous extract of fruit pulp of Eugenia jambolana was investigated in streptozotocin (45 mg/kg body weight)-induced severely diabetic rats (FBG > or = 300 mg/dl). For isolation of active principle, crude aqueous extract of E. jambolana fruit pulp wa...
journal_title：Indian journal of biochemistry & biophysics
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
