Abstract:
:Recent studies have shown that up to 90% of viral vectors could disseminate to normal organs following intratumoral infusion. The amount of dissemination might be dependent on the infusion conditions. Therefore, we investigated the effects of infusion rate, volume, and dose on transgene expression in liver and tumor tissues after intratumoral infusion of an adenoviral vector encoding luciferase. Luciferase expression was determined through bioluminescence intensity measurement. We observed that the luciferase expression in the liver was independent of the infusion rate but increased with the infusion dose, whereas the luciferase expression in the tumor was a bell-shaped function of the infusion rate. The latter observation was consistent with the distribution pattern of Evans blue-labeled albumin after its solution was infused into tumors at the same infusion rates. We also observed that the infusion volume could affect luciferase expression in the tumor but not in the liver. These observations implied that virus dissemination was determined mainly by the infusion dose, whereas the amount of transgene expression in the tumor depended on the distribution volume of viral vectors in the tumor as well as the infusion dose.
journal_name
Mol Cancer Therjournal_title
Molecular cancer therapeuticsauthors
Wang Y,Wang H,Li CY,Yuan Fdoi
10.1158/1535-7163.MCT-05-0266keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
362-6issue
2eissn
1535-7163issn
1538-8514pii
5/2/362journal_volume
5pub_type
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