Abstract：
:The gene mutated in the mouse open brain (opb) phenotype antagonizes sonic hedgehog-mediated signaling and encodes a small GTPase of the Rab family, Rab23. To date, the brain expression profile and exact mechanism of function of the Rab23 protein has remained unknown. Specific antibodies generated against Rab23 showed that the protein is highly enriched in the adult rodent brain and present in low levels in multiple tissues of the adult rodent. Rab23 is found in the cytosol as well as being associated with the plasma and endosomal membranes. In the adult mouse brain, Rab23 is found in betaIII tubulin (TuJ) positive neuronal cell bodies and are most prominent in the cortex, hypothalamus and the cerebellum. It is, however, absent from glial fibrillary acidic protein (GFAP) positive astrocytes or CNPase positive oligodendrocytes. Despite the plasma membrane/endosomal membrane localization of Rab23, neither overexpression of the GTP-restricted nor the GDP-bound mutant forms affect internalization of transferrin or epidermal growth factor. Exogenous overexpression of Rab23 or its mutants also did not affect the morphological differentiation of thalamic neurons in culture. Expression of Rab23 in the adult brain is suggestive, however, of having a postnatal function beyond its role in embryonic development.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Guo A,Wang T,Ng EL,Aulia S,Chong KH,Teng FY,Wang Y,Tang BLdoi
10.1002/jnr.20788keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
1118-27issue
6eissn
0360-4012issn
1097-4547journal_volume
83pub_type
杂志文章abstract：:Oligodendrocytes have a high rate of synthetic activity and produce vast amounts of myelin. The membrane production requires specific sorting and transport processes and structural support. In culture, oligodendrocytes extend flat membranous sheets containing an extensive cytoskeletal network of microtubules (MTs) and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/(sici)1097-4547(20000101)59:1<11::aid-jnr2
更新日期：2000-01-01 00:00:00
abstract：:The inherited deficiency of galactosylceramide beta-galactosidase (E.C. 3.2.1.46: galactocerebrosidase) activity results in globoid cell leukodystrophy in humans (Krabbe disease) and in mice (twitcher mutant). To determine whether Krabbe patients' cells complement twitcher cells to produce, in hybrid combination, grea...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270406
更新日期：1990-12-01 00:00:00
abstract：:The neonatal lesion with 6-hydroxydopamine (6-OHDA) in rodents induces juvenile hyperactivity and paradoxical hypolocomotor response to psychostimulants, in striking contrast to what is observed when similar lesions are carried out in adults. The early disruption of central dopaminergic pathways is followed by increas...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20245
更新日期：2004-10-15 00:00:00
abstract：:The cellular constituents of the blood-brain barrier (BBB) must make finely tuned, regulatory responses to maintain microvascular homeostasis. The mechanisms by which this task is accomplished are largely unknown. However, it is thought they involve a series of cross-talk mechanisms among endothelial cells (EC), peric...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19990301)55:5<578::AID-JNR
更新日期：1999-03-01 00:00:00
abstract：:Fast-scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5-hydroxytryptamine, 5-HT) and norepinephrine (NE). We report a novel DA-specific biosensor consisting of a car...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24193
更新日期：2018-05-01 00:00:00
abstract：:Molecular and behavioral timekeeping is regulated by the circadian system which includes the brain's suprachiasmatic nucleus (SCN) that translates environmental light information into neuronal and endocrine signals aligning peripheral tissue rhythms to the time of day. Despite the critical role of circadian rhythms in...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24606
更新日期：2021-01-01 00:00:00
abstract：:The rate and direction of neurite growth have been shown in a number of studies to be determined by the distribution of adhesive sites on the growth cone. Recent evidence showing that the application of extrinsic electric fields can redistribute membrane molecules and alter both the rate and direction of neurite growt...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130118
更新日期：1985-01-01 00:00:00
abstract：:Fibroblast growth factors (FGFs) are important regulators of retinal development and survival. We examined the expression and distribution of FGF9 and its preferred receptors FGFR2IIIc and FGFR3IIIc in this tissue. FGF9 transcripts in whole rat retina were detected by RT-PCR but were not present in purified cultured M...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20363
更新日期：2005-02-01 00:00:00
abstract：:We sought to establish a mouse model of subcortical ischemic vascular dementia (SIVD) that develops predominant white matter (WM) injury and cognitive dysfunction induced by chronic cerebral hypoperfusion. Adult C57Bl/6 male (n = 48) mice were subjected to bilateral common carotid artery stenosis with external microco...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21925
更新日期：2009-04-01 00:00:00
abstract：:Cell transplantation offers a potential new treatment for stroke. Animal studies using models that produce ischemic damage in both the striatum and the frontal cortex have shown beneficial effects when hNT cells (postmitotic immature neurons) were transplanted into the ischemic striatum. In this study, we investigated...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20800
更新日期：2006-05-01 00:00:00
abstract：:Cerebrospinal fluid was collected from the lateral ventricles and cisternal space from beagle dogs under light pentobarbital anesthesia. Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglyco-sulfate (MOPEG-SO4) levels in the liquor taken from these two regions were evaluated,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490010513
更新日期：1975-01-01 00:00:00
abstract：:We have developed tendon chitosan tubes having the ability to bind peptides covalently, and the effectiveness of laminin peptides coupled to these tubular wall on nerve regeneration was examined in vivo. Bridge graft implantation (15 mm) into the sciatic nerve of SD rats was carried out using chitosan tubes having a t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10589
更新日期：2003-06-01 00:00:00
abstract：:Neural expression of constitutive hsc70 mRNA and hyperthermia-inducible hsp70 mRNA is examined using radioactive and non-radioactive in situ hybridization procedures. A strong induction of hsp70 mRNA was noted in cell populations in cerebellar layers and in the brainstem which demonstrated expression of mRNA encoding ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490410506
更新日期：1995-08-01 00:00:00
abstract：:Amyloid plaques, found in characteristically large numbers in specific brain areas of Alzheimer's disease (AD) and Down's Syndrome (DS) patients, are composed of a 41-43 amino acid peptide, A4, derived from a transmembrane glycoprotein, amyloid precursor protein (APP). In transformed cells APP has been shown to be cle...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490290115
更新日期：1991-05-01 00:00:00
abstract：:We here reconsider current theories of neural ensembles in the context of recent discoveries about neuronal dendritic physiology. The key physiological observation is that the dendritic plateau potential produces sustained depolarization of the cell body (amplitude 10-20 mV, duration 200-500 ms). Our central hypothesi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24240
更新日期：2018-09-01 00:00:00
abstract：:Calcitonin gene-related peptide (CGRP) is the major product of the calcitonin gene in brain and exerts a number of actions in the central nervous system (CNS). In particular the finding that CGRP affects dopamine (DA) release and metabolism has raised the possibility that it may play a role in several neuropsychiatric...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961101)46:3<316::AID-JNR
更新日期：1996-11-01 00:00:00
abstract：:The incubation of murine thymocytes for 24 hr in the presence of cholinergic agonists induces selective proliferation of PNA- cells as measured by thymidine incorporation. The response of PNA- cells is mediated by the activation of nicotinic receptors. This conclusion is suggested by the inability of the muscarinic ag...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490240305
更新日期：1989-11-01 00:00:00
abstract：:The mutant hypogonadal (hpg) mouse lacks a functioning gene for the neurohormone gonadotropin releasing hormone (GnRH). Previous studies from our laboratory had indicated that the initiation and maintenance of reproductive function in these mice could be brought about by the implantation of normal fetal grafts into ad...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490310121
更新日期：1992-01-01 00:00:00
abstract：:The present study examined trophic activities in normal and injured brain which affect the survival and growth of central neurons in culture. Adult rats received bilateral lesions through the angular bundle, severing projections between the entorhinal cortex and the hippocampus. Ten days later, extracts were prepared ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490180203
更新日期：1987-01-01 00:00:00
abstract：:To further our understanding of the functions of the major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP), and other myelin proteins, such as 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin-associated glycoprotein (MAG), bovine brain myelin was extracted with Triton X-100, and p...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10383
更新日期：2002-10-01 00:00:00
abstract：:Tungstate treatment increases the phosphorylation of glycogen synthase kinase-3beta (GSK3beta) at serine 9, which triggers its inactivation both in cultured neural cells and in vivo. GSK3 phosphorylation is dependent on the activation of extracellular signal-regulated kinases 1/2 (ERK1/2) induced by tungstate. As a co...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20726
更新日期：2006-02-01 00:00:00
abstract：:The purpose of this study was to determine if calpain-induced proteolysis was associated with retinal degeneration or dysfunction in the rat acute ocular hypertensive model. Acute glaucoma was produced by elevation of IOP to 120 mm Hg for 1 hr. Retinal degeneration was evaluated by H&E staining and apoptosis was deter...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20827
更新日期：2006-05-15 00:00:00
abstract：:Calpain, an intracellular cysteine protease, has been widely reported to be involved in neuronal cell death. The purpose of this study is to investigate the role of calpain activation in axonal damage-induced retinal ganglion cell (RGC) death. Twelve-week-old male calpstatin (an endogenous calpain inhibitor) knockout ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22800
更新日期：2012-04-01 00:00:00
abstract：:The present study analyzed the in vitro effects induced by sodium L-lactate on human astrocytes and the SH-SY5Y cell line, when added at concentrations of 5, 10, and 25 mmol/liter. Expression of brain-derived neurotrophic factor (BDNF), inducible nitric oxide synthase (iNOS), and heat shock protein 70 kDa (HSP70) was ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23154
更新日期：2013-02-01 00:00:00
abstract：:Miniature endplate potentials (MEPPs) with slow rising phase can be attributed either to burst of transmitter releases or to distortion of conduction from remote releasing sites. The spontaneous activity of neuromuscular junctions recorded extracellularly at mouse diaphragms using sharp electrodes was analyzed to test...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490310313
更新日期：1992-03-01 00:00:00
abstract：:The 14-3-3 proteins exist predominantly in the brain and may play regulatory roles in cellular processes of growth, differentiation, survival, and apoptosis. The biological functions, however, of the various 14-3-3 isoforms (beta, epsilon, eta, gamma, and zeta) in the brain remain unclear. We have reported previously ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20323
更新日期：2005-01-01 00:00:00
abstract：:The presence of acetylcholinesterase has been detected in the thymus of several species both biochemically and histochemically. In this study we have investigated the molecular forms and the level of this enzyme in separate compartments of the murine thymus and in different thymocyte subpopulations. Similar levels of ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490290210
更新日期：1991-06-01 00:00:00
abstract：:After hemisection of the spinal cord in rats, alterations in amino acid incorporation into protein have been observed in brain and spinal cord. Proteins mediating these changes have been studied in the present experiment. Male, Long-Evans hooded rats were given either a laminectomy and dura incision (sham) at spinal s...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490050108
更新日期：1980-01-01 00:00:00
abstract：:Although the expression of hepatocyte growth factor (HGF) and its receptor, proto-oncogene c-met, has been demonstrated in the central nervous system (CNS), the function of HGF in the CNS was not fully understood. In the present studies, we determined the effects of HGF on neuronal development in neocortical explant a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960301)43:5<554::AID-JNR
更新日期：1996-03-01 00:00:00
abstract：:The remarkable quantities of myelin membrane produced by oligodendrocytes has led us to examine the mechanisms involved in the sorting and transport of proteins and lipids during myelinogenesis. Noting that it has been proposed that proteins destined for the apical surface of polarized epithelial cells co-cluster with...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490420316
更新日期：1995-10-15 00:00:00
