Abstract:
:Micronucleus induction by the diarrhetic shellfish toxin okadaic acid (OA) was investigated in two intestinal models, cultured human Caco-2 cells and colon epithelial cells of mice treated in vivo. Exposure to OA for 4 and 24 h induced dose-responsive increases in the frequency of micronucleated Caco-2 cells; the minimum OA doses increasing micronucleus frequency were 20 nM for the 4 h treatment and 5 nM for the 24 h treatment. OA treatment of Caco-2 cells also resulted in dose- and time-dependent increases in mitotic arrest and multinucleated cells. Two experiments were conducted in which mice were treated with single oral gavages of 435-610 and 115-1341 microg/kg OA. In the first experiment, samples were taken 24 h after the treatment, and the frequencies of both micronucleated and mitotic gut cells were increased after treatment with 525 microg/kg OA. In the second experiment, no increases in micronucleus frequency were detected at 24, 36, or 48 h following OA doses of 230 and 115 microg/kg; however, an increase in the mitotic index was observed 36 h after a gavage with 115 microg/kg OA. In this experiment, doses higher than 230 microg/kg were rapidly lethal to the mice. Immunohistology with monoclonal OA antibodies showed that OA was distributed into the liver at all the sampling times and in the small intestine at 24 and 36 h; OA was not detected in the colon. In addition, the TUNEL assay indicated that OA induced apoptosis in mouse ileum, liver, and kidney. The results of our investigations suggest that OA is aneugenic in Caco-2 cells, whereas the in vivo data were inconclusive. Further studies should be performed in mice using intragastric doses of 230-525 microg/kg OA. Moreover, the apoptosis and cell proliferation results indicate that OA can reach organs other than colon, indicating further evaluation of the genotoxic potential of OA in these organs is warranted.
journal_name
Environ Toxicoljournal_title
Environmental toxicologyauthors
Le Hégarat L,Jacquin AG,Bazin E,Fessard Vdoi
10.1002/tox.20154keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
55-64issue
1eissn
1520-4081issn
1522-7278journal_volume
21pub_type
杂志文章abstract::The prostate is an accessory reproductive gland that is sensitive to the action of exogenous compounds known as endocrine disrupters that alter normal hormonal function. Finasteride is a widely used chemical that acts to inhibit the conversion of testosterone in its most active form, dihydrotestosterone. It is known t...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22838
更新日期:2020-01-01 00:00:00
abstract::Doxorubicin (DOX)-induced cardiomyopathy is a lethal disease. DOX-induced cardiotoxic effects are attributed towards increased redox status and apoptotic signaling. In this study, we show that genistein offers protection against DOX-induced cardio toxicity in the mice model. DOX-mediated increase in serum cardiac trop...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22730
更新日期:2019-05-01 00:00:00
abstract::Toxicity of lead on male reproductive functions has raised wide public concern as environmental lead contamination remains common worldwide. Conflicting and controversial data are available regarding effects of lead on male fertility. More importantly, our knowledge on effects of lead on sperm DNA integrity is signifi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22545
更新日期:2018-05-01 00:00:00
abstract::Metal oxide nanoparticles and carbon nanoparticles, as common nanoparticles (NPs), can cause autophagy in certain cells, which will lead to biohealth risk issues. This study determined the difference in autophagy induced by zinc oxide nanoparticles (ZnO NPs) and single-walled carbon nanotubes (SWCNTs) in respiratory e...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.23029
更新日期:2021-02-01 00:00:00
abstract::Phenols are environmentally important compounds that are widely used in agriculture and industry. Thus, phenols are of interest to environmental toxicologists, which has led to the development of several quantitative structure-activity relationship (QSAR) models. The most successful QSARs have been established for com...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10040
更新日期:2002-01-01 00:00:00
abstract::Triclosan, a commonly used antimicrobial compound, has been measured in aquatic systems worldwide. This study exposed marine species to triclosan to examine effects primarily on survival and to investigate the formation of the degradation product, methyl-triclosan, in the estuarine environment. Acute toxicity was asse...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20327
更新日期:2008-04-01 00:00:00
abstract::The chronic effect of p-nonylphenol on survival and reproduction for two generations of the freshwater cladoceran Daphnia galeata was examined by life table experiments. The effects on survival and reproduction were used as the intrinsic rate of natural increase, r, with the Euler-Lotka equation and were analyzed with...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10083
更新日期:2002-10-01 00:00:00
abstract::Culture-independent methods employed in fungal genetic studies using in vitro amplification (PCR) and analysis of specific genes or gene fragments have proved to be useful for detection, identification, and molecular taxonomy of various plant pathogens including Fusarium spp. This approach may be faster than culture-d...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20193
更新日期:2006-08-01 00:00:00
abstract::The aim of this study is to determine the chemical composition, and evaluate the genotoxic, and anti-growth potency of the methanol extracts of lichen species Hypogymnia physodes (L.) Nyl. (HPE). Anti-growth effect was tested in two different human breast cancer cell lines (MCF-7 and MDA-MB-231) by the MTT and ATP via...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21809
更新日期:2014-05-01 00:00:00
abstract::In recent years cyanobacteria blooms have become a severe problem in Taihu Lake, a large shallow eutrophic lake in China. Microcystins produced by certain genera of cyanobacteria can affect public health in this area because of their acute and chronic toxic effects. In this study, samples of cyanobacteria were collect...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20178
更新日期:2006-06-01 00:00:00
abstract::Environmental estrogens are major cause of endocrine disruption in vertebrates, including aquatic organisms. Teleosts are valuable and popular models for studying the effects of endocrine disrupting chemicals (EDCs) in the environment. In the present study, we investigated the changes caused by exposure to the synthet...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.21920
更新日期:2015-04-01 00:00:00
abstract::A 7 day in situ assay to assess sediment toxicity was carried out per replicate in two sites from Guadalete Estuary (Guad1 and Guad2) (South-West, Spain) using the polychaete Arenicola marina (n = 20) in benthic cages. After the exposure period, a battery of biomarkers were determined to asses the sediment toxicity. F...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20416
更新日期:2008-10-01 00:00:00
abstract::Recent interest in adverse effects of infrasound on organisms arises from health concerns. We assessed the association between infrasound exposure of 5 Hz at 130 dB and changes of cardiac ultrastructure and function in rats. Thirty-two Sprague-Dawley rats were randomized into control, 1, 7, and 14 days groups for 2 h ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20244
更新日期:2007-04-01 00:00:00
abstract::Cardiomyocyte apoptosis is the major risk factor for the development of heart failure (HF). The purpose of this study was to evaluate the effects of Gamma-aminobutyric acid (GABA) tea on hypertension-induced cardiac apoptotic pathways in spontaneously hypertensive rats (SHR). In order to reveal the mechanisms, 36 male...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22565
更新日期:2018-07-01 00:00:00
abstract::Bisphenol A (BPA) has been widely reported to exert endocrine disrupting effects, including the induction of adipogenesis in cultured preadipocytes and intact animals. Because of the potential harm to human health, BPA is being substituted by structurally related bisphenols. Whether or not such BPA analogues are safe ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22889
更新日期:2020-05-01 00:00:00
abstract::Methoxychlor (MXC), an organochloride insecticide, is a potent toxicant-targeting female reproductive system and known to cause follicular atresia by inducing apoptosis within granulosa cells. Oxidative stress plays a pivotal role in apoptosis; thus, this study focuses on the ameliorative action of N-acetyl cysteine (...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22221
更新日期:2017-01-01 00:00:00
abstract::The acute toxicity of fatty acids (C14 to C18) commonly found in wood was determined by the standard algal growth inhibition test using the freshwater green alga Selenastrum capricornutum. Toxicity, quantified as IC50 values, varied depending on the number of total carbons and double bonds. Of the tested acids, oleic ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10127
更新日期:2003-10-01 00:00:00
abstract::Melanoma is the leading cause of death from skin disease due to its propensity for metastasis. Studies have shown that integrin-mediated focal adhesion kinase (FAK) signal pathway is implicated in cell proliferation, survival and metastasis of tumor cells. Our previous results indicated that diallyl trisulfide (DATS) ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22445
更新日期:2017-11-01 00:00:00
abstract::Structure-activity relationships for oestrogenicity were developed based on 120 aromatic chemicals evaluated in the Saccharomyces cerevisiae-based Lac-Z reporter assay. Relative gene activation was compared to 17 beta-estradiol and varied over eight orders of magnitude. Analysis of the data compared to 17 beta-estradi...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.10027
更新日期:2002-02-01 00:00:00
abstract::Ouabain, a cardiotonic steroid, was used for the treatment of heart failure and atrial fibrillation and induces cancer cell apoptosis in many human cancer cells including human leukemia cells. However, there are no reports to show the effects on immune responses in a leukemia mouse model. In this study, WEHI-3 cell ge...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22732
更新日期:2019-05-01 00:00:00
abstract::Widespread occupational and environmental exposure to benzene is unavoidable and poses a public health threat. Studies of potential interventions to prevent or relieve benzene toxicity are, thus, essential. Research has shown l-carnitine (LC) has beneficial effects against various pathological processes and diseases. ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22939
更新日期:2020-10-01 00:00:00
abstract::Particulate matter the environmental toxicant, with a diameter less than or equal to 2.5 μm (PM2.5 ) is a common cause of several respiratory diseases. In recent years, several studies have suggested that PM2.5 can influence diverse diseases, such as respiratory diseases, cardiovascular diseases, metabolic diseases, d...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22591
更新日期:2018-11-01 00:00:00
abstract::Tributyltin (TBT), a well-known endocrine disruptor, is an omnipresent environmental pollutant and is explicitly used in many industrial applications. Previously we have shown its neurotoxic potential on cerebral cortex of male Wistar rats. As the effect of TBT on other brain regions is not known, we planned this stud...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22009
更新日期:2015-12-01 00:00:00
abstract::So far, little is known on the distribution of hepatotoxic microcystin (MC) in various organs of bivalves, and there is no study on MC accumulation in bivalves from Chinese waters. Distribution pattern and seasonal dynamics of MC-LR, -YR and -RR in various organs (hepatopancreas, intestine, visceral mass, gill, foot, ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20146
更新日期:2005-12-01 00:00:00
abstract::Seasonal changes in developmental stages, condition factor (CF), gonadosomatic index, and plasma vitellogenin (Vtg) concentrations in male and female hornyhead turbot were examined at the wastewater outfall (T1) of the Orange County Sanitation District, and two farfield sites T11 (7.7 km northwest of the outfall) and ...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20287
更新日期:2007-10-01 00:00:00
abstract::Smoking increases the risk of cardiovascular disorders and leads to damage caused by inflammation and oxidative stress. The actin cytoskeleton is a key player in the response to inflammatory stimuli and is an early target of cellular oxidative stress. The purpose of this study was to investigate the changes in actin c...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22133
更新日期:2016-11-01 00:00:00
abstract::Polychlorinated biphenyls (PCBs) are a class of organic pollutants that have been linked to pancreatic disease. However, their role in affecting the exocrine function of pancreas and the underlying mechanism remains elusive. In the present study, male C57 mice were treated with Aroclor 1254, a commercially available P...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22079
更新日期:2016-06-01 00:00:00
abstract::Acute toxicities of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) were tested on four freshwater species and three plant species. PFOS was more toxic than PFOA for all species tested in this study. Similar time-response patterns of PFOS and PFOA toxicity were observed for each tested species. Valu...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.20396
更新日期:2009-02-01 00:00:00
abstract::Chronic inhalation bioassays in rodents are used to assess pulmonary carcinogenicity for purposes of hazard identification and potentially for risk characterization. Numerous studies have been confirmed that exposure to titanium dioxide nanoparticles (TiO2 NPs) may result in chronic pulmonary inflammation in both mice...
journal_title:Environmental toxicology
pub_type: 杂志文章,收录出版
doi:10.1002/tox.22393
更新日期:2017-05-01 00:00:00
abstract::Although triphenyltin (TPT) compounds are ubiquitous pollutants in urbanised coastal environments in Asian regions, their toxicities to marine organisms are still poorly known. This study was designed to investigate the toxicity of triphenyltin chloride (TPTCl) on the rotifer Brachionus koreanus across different level...
journal_title:Environmental toxicology
pub_type: 杂志文章
doi:10.1002/tox.22018
更新日期:2016-01-01 00:00:00