当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > A transgenic approach for RNA interference-based genetic screening in mice.

A transgenic approach for RNA interference-based genetic screening in mice.

Abstract：

:Genetic screening is the most powerful method through which to uncover gene function. It has been applied very successfully in lower organisms but seldom attempted in mammalian species because of their long generation time. In this study, we exploit RNA interference (RNAi) for its potential use in genetic screening in mice. We show that RNAi-induced gene knockdown can be generated through introducing small hairpin RNA-expressing constructs into the mouse as transgenes via conventional pronuclear injection. The knockdown effect can be transmitted for many generations in these transgenic animals. In a small-scale screening for developmental defects in the kidney, we uncovered a potential role of Id4 in the formation of the renal medulla. Our results demonstrate the feasibility of using RNAi for genetic screening in mice.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Peng S,York JP,Zhang P

doi

10.1073/pnas.0511034103

keywords:

subject

Has Abstract

pub_date

2006-02-14 00:00:00

pages

2252-6

issue

7

eissn

0027-8424

issn

1091-6490

pii

0511034103

journal_volume

103

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Heritability of lobar brain volumes in twins supports genetic models of cerebral laterality and handedness.

    abstract：:Although the left and right human cerebral hemispheres differ both functionally and anatomically, little is known about the environmental or genetic factors that govern central nervous system asymmetry. Nevertheless, cerebral asymmetry is strongly correlated with handedness, and handedness does have a significant gene...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.052494999

    authors： Geschwind DH,Miller BL,DeCarli C,Carmelli D

    更新日期：2002-03-05 00:00:00

  • CTIP2 is a negative regulator of P-TEFb.

    abstract：:The positive transcription elongation factor b (P-TEFb) is involved in physiological and pathological events including inflammation, cancer, AIDS, and cardiac hypertrophy. The balance between its active and inactive form is tightly controlled to ensure cellular integrity. We report that the transcriptional repressor C...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1220136110

    authors： Cherrier T,Le Douce V,Eilebrecht S,Riclet R,Marban C,Dequiedt F,Goumon Y,Paillart JC,Mericskay M,Parlakian A,Bausero P,Abbas W,Herbein G,Kurdistani SK,Grana X,Van Driessche B,Schwartz C,Candolfi E,Benecke AG,Van Lin

    更新日期：2013-07-30 00:00:00

  • The derivation of ecological relationships from physical and chemical principles.

    abstract：:Organic reaction networks are formalized by representing chemical species as points in a space and representing reactions by connectivity rules. Using the generalized network, the behavior of the system is investigated under conditions of electronically exciting input and output to a thermal reservoir. Under steady-st...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.6.2335

    authors： Morowitz HJ

    更新日期：1974-06-01 00:00:00

  • Aire employs a histone-binding module to mediate immunological tolerance, linking chromatin regulation with organ-specific autoimmunity.

    abstract：:Aire induces ectopic expression of peripheral tissue antigens (PTAs) in thymic medullary epithelial cells, which promotes immunological tolerance. Beginning with a broad screen of histone peptides, we demonstrate that the mechanism by which this single factor controls the transcription of thousands of genes involves r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808470105

    authors： Koh AS,Kuo AJ,Park SY,Cheung P,Abramson J,Bua D,Carney D,Shoelson SE,Gozani O,Kingston RE,Benoist C,Mathis D

    更新日期：2008-10-14 00:00:00

  • Interaction of pigeon cytochrome c-(43-58) peptide analogs with either T cell antigen receptor or I-Ab molecule.

    abstract：:We determined that a pigeon cytochrome c-derived peptide, p43-58, possesses two anchor residues, 46 and 54, for binding with the I-Ab molecule that are compatible to the position 1 (P1) and position 9 (P9) of the core region in the major histocompatibility complex (MHC) class II binding peptides, respectively. In the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.22.12047

    authors： Itoh Y,Kajino K,Ogasawara K,Takahashi A,Namba K,Negishi I,Matsuki N,Iwabuchi K,Kakinuma M,Good RA,Onoé K

    更新日期：1997-10-28 00:00:00

  • Polymorphism of HLA-DR beta chains in DR4, -7, and -9 haplotypes: implications for the mechanisms of allelic variation.

    abstract：:We have isolated and sequenced cDNA clones corresponding to the DR beta 1 and DR beta 2 loci from two homozygous B-cell lines typed as DR7 (Burkhart) and DR9 (ISK). These nucleotide sequences were compared to beta 1 and beta 2 chains of other DR haplotypes. The first-domain sequences of beta 2 chains are identical in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.23.9149

    authors： Gregersen PK,Moriuchi T,Karr RW,Obata F,Moriuchi J,Maccari J,Goldberg D,Winchester RJ,Silver J

    更新日期：1986-12-01 00:00:00

  • Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis.

    abstract：:Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated by various neuropeptides. We cloned the gene and analyzed the expression of the prothoracicostatic peptide, a unique neuropeptide shown to suppress the production of ecdysteroids in the prothoracic gland ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0907471107

    authors： Yamanaka N,Hua YJ,Roller L,Spalovská-Valachová I,Mizoguchi A,Kataoka H,Tanaka Y

    更新日期：2010-02-02 00:00:00

  • Skeletal troponin C reduces contractile sensitivity to acidosis in cardiac myocytes from transgenic mice.

    abstract：:Depressed contractile function plays a primary role in the pathophysiology of acute myocardial ischemia. Intracellular acidification is an important factor underlying the inhibition of force production in the ischemic myocardium. The effect of acidosis to depress contractility is markedly greater in cardiac as compare...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.19.9036

    authors： Metzger JM,Parmacek MS,Barr E,Pasyk K,Lin WI,Cochrane KL,Field LJ,Leiden JM

    更新日期：1993-10-01 00:00:00

  • Capturing microRNA targets using an RNA-induced silencing complex (RISC)-trap approach.

    abstract：:Identifying targets is critical for understanding the biological effects of microRNA (miRNA) expression. The challenge lies in characterizing the cohort of targets for a specific miRNA, especially when targets are being actively down-regulated in miRNA- RNA-induced silencing complex (RISC)-messengerRNA (mRNA) complexe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1218887109

    authors： Cambronne XA,Shen R,Auer PL,Goodman RH

    更新日期：2012-12-11 00:00:00

  • Impact and cost-effectiveness of new tuberculosis vaccines in low- and middle-income countries.

    abstract：:To help reach the target of tuberculosis (TB) disease elimination by 2050, vaccine development needs to occur now. We estimated the impact and cost-effectiveness of potential TB vaccines in low- and middle-income countries using an age-structured transmission model. New vaccines were assumed to be available in 2024, t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1404386111

    authors： Knight GM,Griffiths UK,Sumner T,Laurence YV,Gheorghe A,Vassall A,Glaziou P,White RG

    更新日期：2014-10-28 00:00:00

  • Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach.

    abstract：:Expression of the breast and ovarian cancer susceptibility gene BRCA1 is down-regulated in sporadic breast and ovarian cancer cases. Therefore, the identification of genes involved in the regulation of BRCA1 expression might lead to new insights into the pathogenesis and treatment of these tumors. In the present study...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.98.1.130

    authors： Beger C,Pierce LN,Kruger M,Marcusson EG,Robbins JM,Welcsh P,Welch PJ,Welte K,King MC,Barber JR,Wong-Staal F

    更新日期：2001-01-02 00:00:00

  • Reversible, coenzyme-A-mediated inactivation of biosynthetic condensing enzymes in yeast: a possible regulatory mechanism.

    abstract：:alpha-Isopropylmalate synthase [3-hydroxy-4-methyl-3-carboxyvalerate 2-oxo-3-methylbutyrate-lyase (CoA-acetylating); EC 4.1.3.12], the enzyme catalyzing the first committed step in leucine biosynthesis, and homocitrate synthase [3-hydroxy-3-carboxyadipate 2-oxoglutarate-lyase (CoA-acetylating); EC 4.1.3.21], the first...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.5.1802

    authors： Tracy JW,Kohlhaw GB

    更新日期：1975-05-01 00:00:00

  • Exit from G0 and entry into the cell cycle of cells expressing p21Sdi1 antisense RNA.

    abstract：:p21Sdi1 (also known as Cip1 and Waf1), an inhibitor of DNA synthesis cloned from senescent human fibroblasts, is an inhibitor of G1 cyclin-dependent kinases (Cdks) in vitro and is transcriptionally regulated by wild-type p53. In addition, p21Sdi1 has been found to inhibit DNA replication by direct interaction with pro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.10.4352

    authors： Nakanishi M,Adami GR,Robetorye RS,Noda A,Venable SF,Dimitrov D,Pereira-Smith OM,Smith JR

    更新日期：1995-05-09 00:00:00

  • Stable nematic droplets with handles.

    abstract：:We stabilize nematic droplets with handles against surface tension-driven instabilities, using a yield-stress material as outer fluid, and study the complex nematic textures and defect structures that result from the competition between topological constraints and the elasticity of the nematic liquid crystal. We uncov...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1221380110

    authors： Pairam E,Vallamkondu J,Koning V,van Zuiden BC,Ellis PW,Bates MA,Vitelli V,Fernandez-Nieves A

    更新日期：2013-06-04 00:00:00

  • Modeling civil violence: an agent-based computational approach.

    abstract：:This article presents an agent-based computational model of civil violence. Two variants of the civil violence model are presented. In the first a central authority seeks to suppress decentralized rebellion. In the second a central authority seeks to suppress communal violence between two warring ethnic groups. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.092080199

    authors： Epstein JM

    更新日期：2002-05-14 00:00:00

  • 11q23 translocations split the "AT-hook" cruciform DNA-binding region and the transcriptional repression domain from the activation domain of the mixed-lineage leukemia (MLL) gene.

    abstract：:Translocations involving chromosome band 11q23, found in acute lymphoid and myeloid leukemias, disrupt the MLL gene. This gene encodes a putative transcription factor with homology to the zinc fingers and other domains of the Drosophila trithorax gene product and to the "AT-hook" motif of high mobility group proteins....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.22.10610

    authors： Zeleznik-Le NJ,Harden AM,Rowley JD

    更新日期：1994-10-25 00:00:00

  • The novel PII-interactor PirC identifies phosphoglycerate mutase as key control point of carbon storage metabolism in cyanobacteria.

    abstract：:Nitrogen limitation imposes a major transition in the lifestyle of nondiazotrophic cyanobacteria that is controlled by a complex interplay of regulatory factors involving the pervasive signal processor PII Immediately upon nitrogen limitation, newly fixed carbon is redirected toward glycogen synthesis. How the metabol...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2019988118

    authors： Orthwein T,Scholl J,Spät P,Lucius S,Koch M,Macek B,Hagemann M,Forchhammer K

    更新日期：2021-02-09 00:00:00

  • Persistence of deeply sourced iron in the Pacific Ocean.

    abstract：:Biological carbon fixation is limited by the supply of Fe in vast regions of the global ocean. Dissolved Fe in seawater is primarily sourced from continental mineral dust, submarine hydrothermalism, and sediment dissolution along continental margins. However, the relative contributions of these three sources to the Fe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1420188112

    authors： Horner TJ,Williams HM,Hein JR,Saito MA,Burton KW,Halliday AN,Nielsen SG

    更新日期：2015-02-03 00:00:00

  • Human brain glial cells synthesize thrombospondin.

    abstract：:Thrombospondin, a 450-kDa multinodular glycoprotein with lectin-type activity, is found in human platelets, endothelial cells, fibroblasts, smooth muscle cells, monocytes, and granular pneumocytes. Thrombospondin interacts with heparin, fibrinogen, fibronectin, collagen, histidine-rich glycoprotein, and plasminogen. R...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.9.2904

    authors： Asch AS,Leung LL,Shapiro J,Nachman RL

    更新日期：1986-05-01 00:00:00

  • Evolutionary profiles from the QR factorization of multiple sequence alignments.

    abstract：:We present an algorithm to generate complete evolutionary profiles that represent the topology of the molecular phylogenetic tree of the homologous group. The method, based on the multidimensional QR factorization of numerically encoded multiple sequence alignments, removes redundancy from the alignments and orders th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0409715102

    authors： Sethi A,O'Donoghue P,Luthey-Schulten Z

    更新日期：2005-03-15 00:00:00

  • Neural agrin controls acetylcholine receptor stability in skeletal muscle fibers.

    abstract：:At mammalian neuromuscular junctions (NMJs), innervation induces and maintains the metabolic stability of acetylcholine receptors (AChRs). To explore whether neural agrin may cause similar receptor stabilization, we injected neural agrin cDNA of increasing transfection efficiencies into denervated adult rat soleus (SO...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.171539698

    authors： Bezakova G,Rabben I,Sefland I,Fumagalli G,Lømo T

    更新日期：2001-08-14 00:00:00

  • RNA-directed DNA polymerase and virus-induced leukemia in mice.

    abstract：:Rifamycin antibiotics, which inhibit RNA-directed DNA polymerase of Rauscher leuckemia virus, prevent the leukemogenic activity of the virus, and the effect of leukemogenesis correlates with the magnitude of inhibition of the purified enzyme. This inhibition of enzyme activity by rifamycin SV derivatives is due to a r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.5.1298

    authors： Wu AM,Ting RC,Gallo RC

    更新日期：1973-05-01 00:00:00

  • Expression cloning of dSR-CI, a class C macrophage-specific scavenger receptor from Drosophila melanogaster.

    abstract：:Mammalian class A macrophage-specific scavenger receptors (SR-A) exhibit unusually broad binding specificity for a wide variety of polyanionic ligands. The properties of these receptors suggest that they may be involved in atherosclerosis and host defense. We have previously observed a similar receptor activity in Dro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.9.4056

    authors： Pearson A,Lux A,Krieger M

    更新日期：1995-04-25 00:00:00

  • In vitro nonsense suppression in [psi+] and [psi-] cell-free lysates of Saccharomyces cerevisiae.

    abstract：:An homologous in vitro assay for yeast nonsense suppressors was used to examine the effect of the cytoplasmically inherited genetic determinant [psi] on the efficiency of in vitro nonsense suppression. The efficiency of all three types of yeast tRNA-mediated nonsense suppressor (ochre, amber, and UGA) is much greater ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.10.2824

    authors： Tuite MF,Cox BS,McLaughlin CS

    更新日期：1983-05-01 00:00:00

  • Hypoxia-activated cytotoxic agent tirapazamine enhances hepatic artery ligation-induced killing of liver tumor in HBx transgenic mice.

    abstract：:Transarterial chemoembolization (TACE) is the main treatment for intermediate stage hepatocellular carcinoma (HCC) with Barcelona Clinic Liver Cancer classification because of its exclusive arterial blood supply. Although TACE achieves substantial necrosis of the tumor, complete tumor necrosis is uncommon, and the res...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1613466113

    authors： Lin WH,Yeh SH,Yeh KH,Chen KW,Cheng YW,Su TH,Jao P,Ni LC,Chen PJ,Chen DS

    更新日期：2016-10-18 00:00:00

  • Canonical Wnt signaling negatively regulates platelet function.

    abstract：:Wnts regulate important intracellular signaling events, and dysregulation of the Wnt pathway has been linked to human disease. Here, we uncover numerous Wnt canonical effectors in human platelets where Wnts, their receptors, and downstream signaling components have not been previously described. We demonstrate that th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0906268106

    authors： Steele BM,Harper MT,Macaulay IC,Morrell CN,Perez-Tamayo A,Foy M,Habas R,Poole AW,Fitzgerald DJ,Maguire PB

    更新日期：2009-11-24 00:00:00

  • Evolutionary and molecular foundations of multiple contemporary functions of the nitroreductase superfamily.

    abstract：:Insight regarding how diverse enzymatic functions and reactions have evolved from ancestral scaffolds is fundamental to understanding chemical and evolutionary biology, and for the exploitation of enzymes for biotechnology. We undertook an extensive computational analysis using a unique and comprehensive combination o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706849114

    authors： Akiva E,Copp JN,Tokuriki N,Babbitt PC

    更新日期：2017-11-07 00:00:00

  • Neisseria gonorrhoeae-derived heptose elicits an innate immune response and drives HIV-1 expression.

    abstract：:Clinical and epidemiological synergy exists between the globally important sexually transmitted infections, gonorrhea and HIV. Neisseria gonorrhoeae, which causes gonorrhea, is particularly adept at driving HIV-1 expression, but the molecular determinants of this relationship remain undefined. N. gonorrhoeae liberates...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1303738110

    authors： Malott RJ,Keller BO,Gaudet RG,McCaw SE,Lai CC,Dobson-Belaire WN,Hobbs JL,St Michael F,Cox AD,Moraes TF,Gray-Owen SD

    更新日期：2013-06-18 00:00:00

  • Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice.

    abstract：:We have expressed the recombinant squid kinesin head domain in Escherichia coli and studied its interaction with microtubules. The head is active as a microtubule-stimulated ATPase and binds to microtubules, but it does not support microtubule gliding by itself. The head binds to both microtubules and depolymerized tu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.5.1671

    authors： Song YH,Mandelkow E

    更新日期：1993-03-01 00:00:00

  • Switch from Myc/Max to Mad1/Max binding and decrease in histone acetylation at the telomerase reverse transcriptase promoter during differentiation of HL60 cells.

    abstract：:Recent evidence suggests that the Myc and Mad1 proteins are implicated in the regulation of the gene encoding the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. We have analyzed the in vivo interaction between endogenous c-Myc and Mad1 proteins and the hTERT promoter in HL60 cells...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.071043198

    authors： Xu D,Popov N,Hou M,Wang Q,Björkholm M,Gruber A,Menkel AR,Henriksson M

    更新日期：2001-03-27 00:00:00