Potential of the FES-hERL PET reporter gene system -- basic evaluation for gene therapy monitoring.

Abstract:

PURPOSE:In vivo reporter genes can be powerful tools in supporting and ensuring the success of gene therapy. A careful and rational design of a reporter system is essential to realize a noninvasive in vivo reporter gene imaging system applicable for humans. We designed a new in vivo reporter gene imaging system that uses F-18-labeled estradiol (FES) and human estrogen receptor ligand (hERL) binding domain, taking advantage that FES is a radiopharmaceutical already being used for human studies with access to a wide range of tissues, including the brain, and that hERL lacking DNA binding domain can no longer work as a transcription factor, and carried out basic studies to evaluate its potential for gene therapy monitoring. METHODS:We constructed a plasmid (pTIER) to coexpress a model therapeutic gene and the reporter gene hERL and transfected Cos7 cells and examined their uptake of [(3)H]estradiol and FES in culture media. The uptake of FES by mouse calf muscle electroporated with pTIER was also tested. RESULTS:The cells transfected with pTIER took up the radioligands efficiently and specifically in culture media. Also, the mouse calf muscle electroporated with pTIER accumulated a higher amount of FES than did the control. CONCLUSION:The data indicate that our new reporter gene system seems promising for in vivo imaging of gene expression and gene therapy monitoring.

journal_name

Nucl Med Biol

authors

Furukawa T,Lohith TG,Takamatsu S,Mori T,Tanaka T,Fujibayashi Y

doi

10.1016/j.nucmedbio.2005.07.013

keywords:

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

145-51

issue

1

eissn

0969-8051

issn

1872-9614

pii

S0969-8051(05)00200-3

journal_volume

33

pub_type

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