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Application of nonlinear analysis methods for identifying relationships between microbial community structure and groundwater geochemistry.

Abstract：

:The relationship between groundwater geochemistry and microbial community structure can be complex and difficult to assess. We applied nonlinear and generalized linear data analysis methods to relate microbial biomarkers (phospholipids fatty acids, PLFA) to groundwater geochemical characteristics at the Shiprock uranium mill tailings disposal site that is primarily contaminated by uranium, sulfate, and nitrate. First, predictive models were constructed using feedforward artificial neural networks (NN) to predict PLFA classes from geochemistry. To reduce the danger of overfitting, parsimonious NN architectures were selected based on pruning of hidden nodes and elimination of redundant predictor (geochemical) variables. The resulting NN models greatly outperformed the generalized linear models. Sensitivity analysis indicated that tritium, which was indicative of riverine influences, and uranium were important in predicting the distributions of the PLFA classes. In contrast, nitrate concentration and inorganic carbon were least important, and total ionic strength was of intermediate importance. Second, nonlinear principal components (NPC) were extracted from the PLFA data using a variant of the feedforward NN. The NPC grouped the samples according to similar geochemistry. PLFA indicators of Gram-negative bacteria and eukaryotes were associated with the groups of wells with lower levels of contamination. The more contaminated samples contained microbial communities that were predominated by terminally branched saturates and branched monounsaturates that are indicative of metal reducers, actinomycetes, and Gram-positive bacteria. These results indicate that the microbial community at the site is coupled to the geochemistry and knowledge of the geochemistry allows prediction of the community composition.

journal_name

Microb Ecol

journal_title

Microbial ecology

authors

Schryver JC,Brandt CC,Pfiffner SM,Palumbo AV,Peacock AD,White DC,McKinley JP,Long PE

doi

10.1007/s00248-004-0137-0

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

177-88

issue

eissn

0095-3628

issn

1432-184X

journal_volume

pub_type

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Application of nonlinear analysis methods for identifying relationships between microbial community structure and groundwater geochemistry.