Abstract:
:The phototrophic bacterium Rhodobacter capsulatus E1F1 assimilates nitrate under anaerobic phototrophic growth conditions. A 17 kb DNA region encoding the nitrate assimilation (nas) system of this bacterium has been cloned and sequenced. This region includes the genes coding for a putative ABC (ATP-binding cassette)-type nitrate transporter (nasFED) and the structural genes for the enzymes nitrate reductase (nasA), nitrite reductase (nasB) and hydroxylamine reductase (hcp). Three genes code for putative regulatory proteins: a nitrite-sensitive repressor (nsrR), a transcription antiterminator (nasT) and a nitrate sensor (nasS). Other genes probably involved in nitrate assimilation are also present in this region. The sequence analysis of these genes and the biochemical properties of the purified nitrate, nitrite and hydroxylamine reductases are reviewed.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Pino C,Olmo-Mira F,Cabello P,Martínez-Luque M,Castillo F,Roldán MD,Moreno-Vivián Cdoi
10.1042/BST0340127keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
127-9issue
Pt 1eissn
0300-5127issn
1470-8752pii
BST0340127journal_volume
34pub_type
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