A preliminary analysis of platelet von Willebrand factor oligosaccharides.

Abstract:

:Little is known about the carbohydrate structure of the platelet von Willebrand factor (vWf). We have analyzed N-linked oligosaccharides from both plasma and platelet vWf by Fluorophore-Assisted-Carbohydrate Electrophoresis (FACE) and isoelectric focusing before and after digestion of the proteins with neuraminidase. We observed a greater variety of N-linked oligosaccharides derived from plasma vWf than from platelet vWf. Neuraminidase treatment reduced the oligosaccharides from both proteins to only two or three bands and produced significant increases in their isoelectric points.

journal_name

Nagoya J Med Sci

authors

Kagami K,Williams S,Horne M,Gralnick H

keywords:

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

51-6

issue

1-2

eissn

0027-7622

issn

2186-3326

journal_volume

63

pub_type

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