Repair of mandible defect with tissue engineering bone in rabbits.

Abstract:

BACKGROUND:The aim of the present study was to investigate the effect of tissue engineering bone composed of bone marrow-derived osteoblasts and demineralized bone in repairing mandible defect. METHODS:Bone marrow-derived osteoblasts of 20 rabbits were cultured and seeded into scaffold of allogeneic demineralized bone to construct tissue engineering bone graft in vitro, which was used to repair the 10 x 5-mm bone defect made in the same rabbit mandible edge. Implant of demineralized bone alone was as the control. Rabbits were killed according to the schedule: five after 2 weeks, five after 4 weeks, five after 8 weeks, five after 12 weeks, and the implants were harvested for gross, radiographic, and histological observation. RESULTS:New bone formation at the margin region of defect and osteogenesis at the centre were observed in the implant of tissue engineering bone, and the bone formation pattern included osteogenesis, osteoconduction, and osteoinduction. In the implant of demineralized bone alone, the major bone formation pattern was 'creeping substitute'. CONCLUSIONS:The tissue engineering bone graft constructed by autogenous bone marrow-derived osteoblasts and allogeneic demineralized bone was better than demineralized bone alone in bone formation capability, which might be an ideal graft for bone defect repair.

journal_name

ANZ J Surg

journal_title

ANZ journal of surgery

authors

Li Z,Li ZB

doi

10.1111/j.1445-2197.2005.03586.x

keywords:

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

1017-21

issue

11

eissn

1445-1433

issn

1445-2197

pii

ANS3586

journal_volume

75

pub_type

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