Abstract：
:Real-time RT-PCR has been frequently used in quantitative research in molecular biology and bioinformatics. It provides remarkably useful technology to assess expression of genes. Although mathematical models for gene amplification process have been studied, statistical models and methods for data analysis in real-time RT-PCR have received little attention. In this paper, we briefly introduce current mathematical models, and study statistical models for real-time RT-PCR data. We propose a generalized estimation equations (GEE) model that properly reflects the structure of repeated data in RT-PCR experiments for both cross-sectional and longitudinal data. The GEE model takes the correlation between observations within the same subjects into consideration, and prevents from producing false positives or false negatives. We further demonstrate with a set of actual real-time RT-PCR data that different statistical models yield different estimations of fold change and confidence interval. The SAS program for data analysis using the GEE model is provided to facilitate easy computation for non-statistical professionals.
journal_name
Comput Biol Chemjournal_title
Computational biology and chemistryauthors
Fu WJ,Hu J,Spencer T,Carroll R,Wu Gdoi
10.1016/j.compbiolchem.2005.10.005keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
21-6issue
1eissn
1476-9271issn
1476-928Xpii
S1476-9271(05)00106-4journal_volume
30pub_type
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