Exploitation of genomic sequences in a systematic analysis to access how cyanobacteria sense environmental stress.


:The perception and subsequent transduction of environmental signals are primary events in the acclimation of living organisms to changes in their environment. Many of the molecular sensors and transducers of environmental stress cannot be identified by traditional and conventional methods. Therefore, the genomic information has been exploited in a systematic approach to this problem, performing systematic mutagenesis of potential sensors and transducers, namely, histidine kinases and response regulators, respectively, in combination with DNA microarray analysis, to examine the genome-wide expression of genes in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Using targeted mutagenesis, 44 out of the 47 histidine kinases and 42 out of the 45 response regulators of this organism have successfully been inactivated. The resultant mutant libraries were screened by genome-wide DNA microarray analysis and by slot-blot hybridization analysis under various stress and non-stress conditions. Histidine kinases have been identified that perceive and transduce signals of low-temperature, hyperosmotic, and salt stress, as well as manganese deficiency.


J Exp Bot


Murata N,Suzuki I





Has Abstract


2006-01-01 00:00:00














  • Evidence for the presence of photorespiration in desiccation-sensitive leaves of the C4 'resurrection' plant Sporobolus stapfianus during dehydration stress.

    abstract::The possible role of photorespiration as a general stress protection mechanism, and in C(4) plant metabolism, is controversial. In particular, the potential involvement of photorespiration in the acquisition of desiccation tolerance in 'resurrection' plants is unknown. An investigation was carried out into whether pho...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Martinelli T,Whittaker A,Masclaux-Daubresse C,Farrant JM,Brilli F,Loreto F,Vazzana C

    更新日期:2007-01-01 00:00:00

  • Unraveling the genetic architecture of grain size in einkorn wheat through linkage and homology mapping and transcriptomic profiling.

    abstract::Understanding the genetic architecture of grain size is a prerequisite to manipulating grain development and improving the potential crop yield. In this study, we conducted a whole genome-wide quantitative trait locus (QTL) mapping of grain-size-related traits by constructing a high-density genetic map using 109 recom...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Yu K,Liu D,Chen Y,Wang D,Yang W,Yang W,Yin L,Zhang C,Zhao S,Sun J,Liu C,Zhang A

    更新日期:2019-09-24 00:00:00

  • HYL1 is required for establishment of stamen architecture with four microsporangia in Arabidopsis.

    abstract::The stamen produces pollen grains for pollination in higher plants. Coordinated development of four microsporangia in the stamen is essential for normal fertility. The roles of miR165/166-directed pathways in the establishment of adaxial-abaxial polarity have been well defined in leaves. However, the molecular mechani...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lian H,Li X,Liu Z,He Y

    更新日期:2013-08-01 00:00:00

  • Protein lipid modifications and the regulation of ROP GTPase function.

    abstract::In eukaryotes, the RHO superfamily of small G-proteins is implicated in the regulation of cell polarity and growth. Rho of Plants (ROPs)/RACs are plant-specific Rho family proteins that have been shown to regulate cell polarity, auxin transport and responses, ABA signalling, and response to pathogens. A hallmark of RO...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Yalovsky S

    更新日期:2015-03-01 00:00:00

  • Zygnema circumcarinatum UTEX 1559 chloroplast and mitochondrial genomes provide insight into land plant evolution.

    abstract::The complete chloroplast and mitochondrial genomes of Charophyta have shed new light on land plant terrestrialization. Here, we report the organellar genomes of the Zygnema circumcarinatum strain UTEX 1559, and a comparative genomics investigation of 33 plastomes and 18 mitogenomes of Chlorophyta, Charophyta (includin...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Orton LM,Fitzek E,Feng X,Grayburn WS,Mower JP,Liu K,Zhang C,Duvall MR,Yin Y

    更新日期:2020-06-11 00:00:00

  • Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice.

    abstract::The indeterminate gametophyte1 (ig1) mutation was first characterized to modulate female gametophyte development in maize (Zea mays). However, the function of its rice orthologue, OsIG1, remains unknown. For this, we first analysed OsIG1 localization from differential tissues in rice. Real-time quantitative PCR (qRT-P...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zhang J,Tang W,Huang Y,Niu X,Zhao Y,Han Y,Liu Y

    更新日期:2015-01-01 00:00:00

  • Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant.

    abstract::The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Huang C,He W,Guo J,Chang X,Su P,Zhang L

    更新日期:2005-12-01 00:00:00

  • Ribonuclease J is required for chloroplast and embryo development in Arabidopsis.

    abstract::Chloroplasts perform many essential metabolic functions and their proper development is critically important in embryogenesis. However, little is known about how chloroplasts function in embryogenesis and more relevant components need to be characterized. In this study, we show that Arabidopsis Ribonuclease J (RNase J...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Chen H,Zou W,Zhao J

    更新日期:2015-04-01 00:00:00

  • ZmIBH1-1 regulates plant architecture in maize.

    abstract::Leaf angle (LA) is a critical agronomic trait in maize, with more upright leaves allowing higher planting density, leading to more efficient light capture and higher yields. A few genes responsible for variation in LA have been identified by map-based cloning. In this study, we cloned maize ZmIBH1-1, which encodes a b...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cao Y,Zeng H,Ku L,Ren Z,Han Y,Su H,Dou D,Liu H,Dong Y,Zhu F,Li T,Zhao Q,Chen Y

    更新日期:2020-05-30 00:00:00

  • Haem oxygenase-1 is involved in salicylic acid-induced alleviation of oxidative stress due to cadmium stress in Medicago sativa.

    abstract::This work examines the involvement of haem oxygenase-1 (HO-1) in salicylic acid (SA)-induced alleviation of oxidative stress as a result of cadmium (Cd) stress in alfalfa (Medicago sativa L.) seedling roots. CdCl(2) exposure caused severe growth inhibition and Cd accumulation, which were potentiated by pre-treatment w...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Cui W,Li L,Gao Z,Wu H,Xie Y,Shen W

    更新日期:2012-09-01 00:00:00

  • Darwin's foundation for investigating self-incompatibility and the progress toward a physiological model for S-RNase-based SI.

    abstract::Charles Darwin made extensive observations of the pollination biology of a wide variety of plants. He carefully documented the consequences of self-pollination and described species that were self-sterile but that could easily be crossed with other plants of the same species. He believed that compatibility was control...

    journal_title:Journal of experimental botany

    pub_type: 历史文章,杂志文章,评审


    authors: McClure B

    更新日期:2009-01-01 00:00:00

  • Small RNA activity and function in angiosperm gametophytes.

    abstract::Small non-coding RNAs are key post-transcriptional and transcriptional regulators of plant gene expression in angiosperm sporophytes. In recent years, gametophytic small RNAs have also been investigated, predominantly in Arabidopsis male gametophytes, revealing features in common with the sporophyte as well as some su...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Le Trionnaire G,Grant-Downton RT,Kourmpetli S,Dickinson HG,Twell D

    更新日期:2011-03-01 00:00:00

  • VpRFP1, a novel C4C4-type RING finger protein gene from Chinese wild Vitis pseudoreticulata, functions as a transcriptional activator in defence response of grapevine.

    abstract::RING finger proteins comprise a large family and play important roles in regulation of growth and development, hormone signalling, and responses to biotic and abiotic stresses in plants. In this study, the identification and functional characterization of a C4C4-type RING finger protein gene from the Chinese wild grap...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Yu Y,Xu W,Wang S,Xu Y,Li H,Wang Y,Li S

    更新日期:2011-11-01 00:00:00

  • Light and CO2 do not affect the mesophyll conductance to CO2 diffusion in wheat leaves.

    abstract::In C(3) plants, diffusion of CO(2) into leaves is restricted by stomata and subsequently by the intercellular airspaces and liquid phase into chloroplasts. While considerable information exists on the effect of environmental conditions on stomatal conductance (g(s)), little is known on whether the mesophyll conductanc...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Tazoe Y,von Caemmerer S,Badger MR,Evans JR

    更新日期:2009-01-01 00:00:00

  • K+ starvation inhibits water-stress-induced stomatal closure via ethylene synthesis in sunflower plants.

    abstract::The effect of water stress on stomatal closure in sunflower plants has been found to be dependent on K(+) nutrient status. When plants with different internal K(+) content were subjected to a water-stress period, stomatal conductance was reduced more markedly in plants with an adequate K(+) supply than in K(+)-starved...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Benlloch-González M,Romera J,Cristescu S,Harren F,Fournier JM,Benlloch M

    更新日期:2010-02-01 00:00:00

  • Metabolic characterization of loci affecting sensory attributes in tomato allows an assessment of the influence of the levels of primary metabolites and volatile organic contents.

    abstract::Numerous studies have revealed the extent of genetic and phenotypic variation between both species and cultivars of tomato. Using a series of tomato lines resulting from crosses between a cherry tomato and three independent large fruit cultivar (Levovil, VilB, and VilD), extensive profiling of both central primary met...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zanor MI,Rambla JL,Chaïb J,Steppa A,Medina A,Granell A,Fernie AR,Causse M

    更新日期:2009-01-01 00:00:00

  • An Arabidopsis gene encoding a C2H2-domain protein with alternatively spliced transcripts is essential for endosperm development.

    abstract::The endosperm is an essential part of the seed which sustains embryo development and contains storage reserves. Endosperm development involves a series of nuclear divisions in the absence of cytokinesis. In this study, it is shown that a mutant Arabidopsis plant carrying the disrupted At4g24900 gene exhibits severe de...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Lu X,Li Y,Su Y,Liang Q,Meng H,Li S,Shen S,Fan Y,Zhang C

    更新日期:2012-10-01 00:00:00

  • β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.

    abstract::Carboxysomes are bacterial microcompartments (BMCs) that enhance CO2 fixation in all cyanobacteria. Structurally, carboxysome shell proteins are classified according to the type of oligomer formed: hexameric (BMC-H), trimeric (BMC-T) and pentameric (BMC-P) proteins. To understand the forces driving the evolution of th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sommer M,Cai F,Melnicki M,Kerfeld CA

    更新日期:2017-06-01 00:00:00

  • COP1 regulates plant growth and development in response to light at the post-translational level.

    abstract::Photoreceptors perceive different wavelengths of light and transduce light signals downstream via a range of proteins. COP1, an E3 ubiquitin ligase, regulates light signaling by mediating the ubiquitination and subsequent proteasomal degradation of photoreceptors such as phytochromes and cryptochromes, as well as vari...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Kim JY,Song JT,Seo HS

    更新日期:2017-10-13 00:00:00

  • alpha4-Fucosyltransferase is regulated during flower development: increases in activity are targeted to pollen maturation and pollen tube elongation.

    abstract::alpha4-Fucosylation represents a final step of protein N- glycosylation. alpha4-fucosylated N-glycans are thought to be involved in cell-to-cell communication and recognition in primates and plants. Nevertheless, in the plant life cycle, the function of alpha4-fucosylation remains largely unknown. To gain an insight i...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Joly C,Léonard R,Maftah A,Riou-Khamlichi C

    更新日期:2002-06-01 00:00:00

  • Integrating the dynamics of yield traits in rice in response to environmental changes.

    abstract::Reductions in crop yields as a consequence of global climate change threaten worldwide food security. It is therefore imperative to develop high-yielding crop plants that show sustainable production under stress conditions. In order to achieve this aim through breeding or genetic engineering, it is crucial to have a c...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章,评审


    authors: Nutan KK,Rathore RS,Tripathi AK,Mishra M,Pareek A,Singla-Pareek SL

    更新日期:2020-01-07 00:00:00

  • Evidence for long-distance xylem transport of signal peptide activity from tomato roots.

    abstract::Several types of small, endogenous signal peptides are now known to induce a wide range of local and systemic responses in plants, but how such signal peptide activity is transported over long distances remains unclear. In particular, the possible occurrence and root-to-shoot transport of signal peptide activity in th...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Neumann PM

    更新日期:2007-01-01 00:00:00

  • Reporter gene expression reveals precise auxin synthesis sites during fruit and root development in wild strawberry.

    abstract::The critical role of auxin in strawberry fruit set and receptacle enlargement was demonstrated previously. While fertilization is known to trigger auxin biosynthesis, the specific tissue source of fertilization-induced auxin is not well understood. Here, the auxin reporter DR5ver2::GUS was introduced into wild strawbe...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Feng J,Dai C,Luo H,Han Y,Liu Z,Kang C

    更新日期:2019-01-07 00:00:00

  • Discharge of surplus phloem water may be required for normal grape ripening.

    abstract::At the onset of ripening, some fleshy fruits shift the dominant water import pathway from the xylem to the phloem, but the cause for the decline in xylem inflow remains obscure. This study found that xylem-mobile dye movement into grape berries decreased despite transient increases in berry growth and transpiration du...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Zhang Y,Keller M

    更新日期:2017-01-01 00:00:00

  • A bioassay-guided fractionation system to identify endogenous small molecules that activate plasma membrane H+-ATPase activity in Arabidopsis.

    abstract::Plasma membrane (PM) H+-ATPase is essential for plant growth and development. Various environmental stimuli regulate its activity, a process that involves many protein cofactors. However, whether endogenous small molecules play a role in this regulation remains unknown. Here, we describe a bio-guided isolation method ...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Han X,Yang Y,Wu Y,Liu X,Lei X,Guo Y

    更新日期:2017-05-17 00:00:00

  • Viscoelastic properties of cell walls of single living plant cells determined by dynamic nanoindentation.

    abstract::Plant development results from controlled cell divisions, structural modifications, and reorganizations of the cell wall. Thereby, regulation of cell wall behaviour takes place at multiple length scales involving compositional and architectural aspects in addition to various developmental and/or environmental factors....

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Hayot CM,Forouzesh E,Goel A,Avramova Z,Turner JA

    更新日期:2012-04-01 00:00:00

  • Comprehensive QTL mapping survey dissects the complex fruit texture physiology in apple (Malus x domestica Borkh.).

    abstract::Fruit ripening is a complex physiological process in plants whereby cell wall programmed changes occur mainly to promote seed dispersal. Cell wall modification also directly regulates the textural properties, a fundamental aspect of fruit quality. In this study, two full-sib populations of apple, with 'Fuji' as the co...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Longhi S,Moretto M,Viola R,Velasco R,Costa F

    更新日期:2012-02-01 00:00:00

  • PbrMYB169 positively regulates lignification of stone cells in pear fruit.

    abstract::Stone cells negatively affect fruit quality because of their firm and lignified cell walls, so are targets for reduction in pear breeding programmes. However, there is only limited knowledge of the molecular mechanisms underlying the formation of stone cells. Here, we show that PbrMYB169, an R2R3 MYB transcription fac...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Xue C,Yao JL,Xue YS,Su GQ,Wang L,Lin LK,Allan AC,Zhang SL,Wu J

    更新日期:2019-03-27 00:00:00

  • Functional relationship between mTERF4 and GUN1 in retrograde signaling.

    abstract::Plastid-to-nucleus retrograde signaling plays an important role in regulating the expression of photosynthesis-associated nuclear genes (PhANGs) in accordance with physiological demands on chloroplast biogenesis and function. Despite its fundamental importance, little is known about the molecular nature of the plastid...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Sun X,Xu D,Liu Z,Kleine T,Leister D

    更新日期:2016-06-01 00:00:00

  • Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato.

    abstract::Heavy metal pollution often occurs together with organic contaminants. Brassinosteroids (BRs) induce plant tolerance to several abiotic stresses, including phenanthrene (PHE) and cadmium (Cd) stress. However, the role of BRs in PHE+Cd co-contamination-induced stress amelioration is unknown. Here, the interactive effec...

    journal_title:Journal of experimental botany

    pub_type: 杂志文章


    authors: Ahammed GJ,Choudhary SP,Chen S,Xia X,Shi K,Zhou Y,Yu J

    更新日期:2013-01-01 00:00:00